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EMISI DAN ABSORPSI GAS METANA PADA SISTEM PENANAMAN PADI DI AREA TANAH SAWAH Marniati Salim; Hiyal Faizah; I Made Sudiana
Jurnal Riset Kimia Vol. 4 No. 1 (2010): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v4i1.74

Abstract

 To know there are emission and methane absorption, research was conduted by measuring directly gas methane at the rice field and absorption with metanotroph bactery using chromatography gas. From the research, we know that there is methane gas emission at the rice field. Watery land condition, emit methane gas (2,309 mg/L) larger than not watery land (0,059 mg/L). At the same time and paddy age is 2 month, it also emit larger methane gas (1,809 mg/L) than 1 month age paddy (1,758 mg/L) and without paddy (0,697 mg/L), whereas for methane absorption at land sample  T0, T1, T2, T3, shows that with fertilizer given can increase the reduction of injected methane gas. Keywords: Absorption, Metanotroph bacteria, Methane gas emission   
KESIAGAAN PAKAN PADA TERNAK SAPI SKALA KECIL SEBAGAI STRATEGI ADAPTASI TERHADAP PERUBAHAN IKLIM MELALUI PEMANFAATAN BIODIVERSITAS FLORA LOKAL Maman Rahmansyah; Arwan Sugiharto; Atit Kanti; I Made Sudiana
Buletin Peternakan Vol 37, No 2 (2013): BULETIN PETERNAKAN VOL. 37 (2) JUNI 2013
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v37i2.2427

Abstract

The presence of local floral resources as forage is essential, especially for the farm survival in anticipaty the impact of the global climate change. The availability of forage in the dry land ecosystems depends on water supply. However, the climate change has caused the change on rainfall pattern including the initiation of the dry season. The problem should be anticipated by the local farmers’ readiness in providing sufficient feed and their ability in performing the excellent management during all seasons. In some areas of East Bali and Nusa Tenggara (West andEast), dry climate crisis 2004 led to feed scarcity. Therefore, this study was conducted in order to identify the potential forage resources in the arid land. The discussion is focused on the results of survey and secondary data collection. Based on the results, there were 22 plants that could be used as forage which were abundant during rainy season, whereas 15 others were able to be forage and silage during the transitional season and along the dry season. The results also showed that the introduction of silage fermentation technique was required as the way to preserve feed for the supply during the dry season. In this study the role of polymer compounds hydrolyzing microbes and the fermentation process by the GRAS (Generally Recognized as Safe) microbes preceeded the role of Lactobacillus plantarum were observed. The results of this study may become a reference in anticipating the global climate change impacts in dry land areas by the application of functional microbial technology in small local farms’ feed management activities.(Key words: Local floral resources, Dry land climate, Fermented feed, Lactobacillus plantarum)
Isolasi Bakteri Pengakumulasi Poliposfat I Made Sudiana
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2004): June 2004
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i2.2898

Abstract

Polyphosphate accumulating bacteria play  important key role on biological phosphorous removal. Activated sludge originated from wastewater treatment plant for drinking water production acclimated with anaerobic-aerobic phase in sequential batch reactor continuously succeed to establish microbial community that are able to adsorb glucose and acetate under anaerobic condition.  Acinetobacter calcoaceticus S1 was an isolated bacterium from community performing organic substrate absorption. S1 is able to adsorb glucose and acetate under anaerobic condition. During substrate absorption an increase of orthophosphate in bulk solution indicating phosphate released out of cell. Reduction of glycogen and polyhydroxybutirate (PHB) took place under anaerobic phase. S1 is a community member of anaerobic-aerobic system for removal of phosphorous in influent wastewater.
Integrasi Kearifan Lokal Bali dalam Buku Ajar Sekolah Dasar I Made Sudiana; I Gede Sudirgayasa
Jurnal Kajian Bali (Journal of Bali Studies) Vol 5 No 1 (2015): AIR DAN PERUBAHAN SOSIAL DI BALI
Publisher : Pusat Kajian Bali Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (649.066 KB)

Abstract

AbstractIntegration of Balinese local wisdom required to maintainthe noble values of Balinese culture through early education.One of them is to do a primary school textbook developmentthat is integrated with the Balinese local wisdom as analternative. Therefore, this study was conducted to examinea wide range of Balinese local wisdom with regard toprimary school education. This is a development research.Initial data collection is done with the study of documents,interviews, and questionnaires. The elementary school isused as a source document and the respondent amountedto six elementary schools representing urban, suburban,and rural. Based on the preliminary data analysis, prepareda textbook draft. Assessment is done with a texbook draftfeasibility assessment instruments adopted from the bookassessment instruments by National Education StandardsAgency. Analysis of results of the assessment carried outby qualitative descriptive with the help of Microsoft OfficeExcel 2007. Based on the results of research and discussion,it can be concluded, that there are a lot of Balinese localwisdom with values that can be integrated in primary schooleducation. The results also show that the integrated Balineselocal wisdom textbook draft classified as good and feasibleto implement.
Studi Etnobotanik Tanaman Upacara Hindu Bali sebagai Upaya Pelestarian Kearifan Lokal I Ketut Surata; I Wayan Gata; I Made Sudiana
Jurnal Kajian Bali (Journal of Bali Studies) Vol 5 No 2 (2015): BALI DIASPORA DAN KEARIFAN LOKAL
Publisher : Pusat Kajian Bali Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (690.411 KB)

Abstract

AbstractPreservation  the local wisdom of Balinese culture need to continue to do. To begin, the initial knowledge is needed that is able to answer the following questions: How does the local wisdom is obtained? How is this done during scanning to the younger generation? This article tries to answer the above questions with a focus on the study of local wisdom pertaining to ethnobotany of ceremonial  plants on Balinese Hindu communities. Research using exploratory approach. Location of the study includes five Desa Pakraman (Balinese village) in the province of Bali. Inventory data is done through the analysis of documents, interviews, and observations. The resource person is determined by snow ball sampling techniques. Data analysis was done through qualitative critical. The results showed that ethnobotany documentation of Balinese Hindu ceremonial plant, still classified as minimal. Identification is done on the basis of morphological characteristics of the plant. Scanning of knowledge belonging to the younger generation to be done through oral techniques and provides hands-on experience through time of preparation, implementation, and post implementation activities of religious rituals. It takes an attempt of documenting through the media, so that available anytime, anywhere.
Membaca Karya-karya IDK Raka Kusuma dalam Bégal I Made Sudiana
Jurnal Kajian Bali (Journal of Bali Studies) Vol 2 No 2 (2012): BAHASA BALI DALAM PELANGI ILMU
Publisher : Pusat Kajian Bali Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (804.654 KB)

Abstract

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POTENCY OF RHIZOSPHERE BACTERIA TO PROMOTE RICE GROWTH UNDER SALINE CONDITION Sri Widawati; I Made Sudiana
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 23 No. 2 (2016)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (528.765 KB) | DOI: 10.11598/btb.2016.23.2.511

Abstract

Saline soil is a common problem in coastal paddy field, especially in Indonesia. Salinity affects rice growth and the activities of soil functional microbes, including functional bacteria, which play roles in plant growth. Some of these microbes are associated with rice plants and are able to survive under saline condition. The presence of functional microbes is also important to improve soil quality. Nitrogen and phosphate are essential soil nutrients and is available in soil due to the activities of nitrogen-fixing bacteria and free-living plant-associated bacteria.  The objective of the present study was to obtain nitrogen-fixing, phosphate solubilizing and Indole Acetic Acid  (IAA)-producing bacteria that are able to survive and promote the growth of rice under saline conditions. From rice and peanut rhizosphere, Ca-phosphate (Ca-P) solubilizing and nitrogen-fixing bacteria were isolated separately using specific media. Then, the Ca-P solubilizing ability, phosphomonoesterase activity and IAA-producing ability were quantitatively examined. Based on the abilities, 20 strains were selected and identified as Burkholderia cepacia-complex, Burkholderia anthina, Burkholderia cenocepacia, Bacillus cereus-complex (three strains), Achromobacter spanius, Azospirillum sp. (four strains), Azotobacter sp. (three strains), Rhizobium leguminosarum, Rhizobium sp. (two strains), and Pseudomonas sp. (three strains). The inoculation of several single strains or the mixture of the selected strains promoted the growth of rice under saline conditions. These inoculants could be potential as biofertilizer in saline paddy fields.
CHARACTERIZATION OF RHIZOBACTERIA ISOLATES FROM SOIL AND NODULES sri widawati; SULIASIH SULIASIH; Dwi Ningsih Susilowati; Yuki Muramatsu; I Made Sudiana
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 27 No. 3 (2020): BIOTROPIA Vol. 27 No. 3 December 2020
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (290.948 KB) | DOI: 10.11598/btb.0.0.0.1241

Abstract

The plant growth promoting rhizobacteria (PGPR) is a group of bacteria capable of colonizing plants roots, thereby developing a system and improving plants growth and yield. The objectives of the study is to characterize the PGPR activities of several bacterial isolates {in-vitro screening), to examine their activities in stimulating soybean growth (in-vivo screening), and to identify the bacterial species. These were isolated from nodules and soil samples collected from Mount Pancar in Bogor, West Java Province as well as from Bangkirai Hill and Wain River in East Kalimantan, Indonesia. The in-vitro PGPR activity characterization includes the N-fixing ability, ACC-deaminase, indole acetic acid (IAA) production, cellulolytic activity, P-solubilization, Phosphomonoesterase (PME-ase), and nifH-gene detection. The in-vivo PGPR activity with the greenhouse assay was conducted on soybean plant {Glycine max L.). All bacterial isolates were identified using molecular methods based on nucleotide sequence generated from 16S rRNA gene. Three isolates of soil and nodule bacteria with 7 characteristics of PGPR (N2 fixation, ACC-deaminase, cellulolytic activity, IAA production, solubilization index, P available, and PMEase activity) were successfully identified. These isolates were B045 {Klebsiella variicola InaCC B827), B116 (Klebsiella sp. InaCC B833), and B210 {Mangrovibacterplantisponsorlaa.CC B841). The greenhouse assay showed that the plant height, plant dry weight and number of flowers in soybean seedlings significantly increased with Bradyrhispbium sp. strain 4167, then with Klebsiella sp. InaCC B833 and Mangrovibacterplantisponsor InaCC B841. These bacterial isolates which were characterized and screened in-vitro for PGPR potentials and their representative isolates which were identified by 16S rRNA sequence analysis are key factors for selecting PGPR isolates to be commercialized later as bio-stimulant.
DIVERSITY AND ECOLOGICAL PERSPECTIVE OF SOIL YEAST IN GUNUNG HALIMUN NATIONAL PARK*[Khamir Tanah Taman Nasional Gunung Halimun: Keragaman dan Perspektif] Atit Kanti; I Made Sudiana
BERITA BIOLOGI Vol 6, No 1 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i1.1167

Abstract

Taman Nasional Gunung Halimun merupakan hutan hujan tropika yang berada di pulau Jawa. Penelitian ini ditujukan untuk mengetahui populasi dari khamir tanah pada berbagai ketinggian tempat (600 m, 1000 m, dan 1500 m) di atas permukaan laut, dan selanjutnya mempelajari peran ekologi dari khamir, dilakukan dengan mengisolasi dan menganalisa karakter fisiologi terutama dalam mendekomposisi selulosa dan hidrolisa fosfat. Khamir yang diisolasi dari tanah dan daerah perakaran dimurnikan dan ditumbuhkan pada media yang mengandung carboxymethylcellulose (CMC) sebagai sumber karbon utama, dan juga ditumbuhkan pada media pivoskaya untuk mengetahui kemampuan pelarutan Ca3(PO4)2. Populasi khamir pada daerah yang atas sedikit lebih tinggi .Sebanyak 23 isolat khamir yang termasuk dalam marga Debaryomyces, Pichia, Rhodotorula, dan Candida diisolasi dari tanah.15 isolat mempunyai kemampuan menghidrolisa selulosa dan 9 isolat mampu melarutkan Ca3(PO4)2. Khamir ditemukan di tanah dan daerah perakaran dan memegang peran penting dalam degradasi senyawa organik dan mineralisasi fosfat di dalam tanah.
IDENTIFICATION OF YEASTS ISOLATED FROM GUNUNG HALIMUN NATIONAL PARK*[Identifikasi Khamir pada Taman Nasional Gunung Halimun] Atit Kanti; Heddy Julistiono; I Made Sudiana
BERITA BIOLOGI Vol 6, No 1 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i1.1177

Abstract

Dua puluh sembilan isolat khamir diisolasi dari tanah Taman Nasional Gunung Halimun. Sumber isolat berasal dari batang pohon lapuk, akar lapuk yang diambil dari Gunung Botol, Cikaniki, dan Cipta Rasa untuk dipelajari aspek taksonominya. Berdasarkan atas karakter morfologi dan fisiologi, isolat-isolat tersebut digolongkan kepada kelompok ascomyceteous, basidiomyceteous dan imperfect khamir. Selanjutnya ketiga golongan tersebut dimasukkan ke dalam sepuluh kelompok (Kelompok 1 sampai X). Dari 29 isolat tersebut, 7 isolat dimasukkan ke dalam kelompok I diindentifikasi sebagai Debaryomyces hansenii, 6 dalam kelompok II sebagai Candida sp, 2 dalam kelompok III sebagai Pichia membranafaciens, 5 isolat dalam kelompok IV sebagai Candida galacta, 1 dalam kelompok V sebagai Candida sake, 4 dalam kelompok VI sebagai Cryptococcus humicolus, 1 dalam kelompok VII sebagai Rhodotorula minuta, 1 dalam kelompok VIII sebagai Candida sp, 1 dalam kelompok Candida sp, dan 1 dalam kelompok X dalam Candida sp. Macam sampel tampaknya tidak berpengaruh kepada keragaman jenis khamir seperti ditunjukkan oleh jenis yang sama diisolasi dari berbagai jenis sampel. Dari banyaknya jenis khamir yang diisolasi menunjukkan bahwa keragaman jenis khamir di Taman Nasional Gunung Halimun tergolong tinggi.