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AKTIVITAS CMC-ase KHAMIR Candida sp. YANG DIISOLASI DARI TANAH KEBUN BIOLOGI WAMENA, PAPUA Atit Kanti; I Made Sudiana
BERITA BIOLOGI Vol 6, No 5 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i5.1190

Abstract

Cellulolytic Candida sp.was isolated from Wamena, Papua.The strain was able to grow in media with carboxymethyl cellulose as a sole carbon source implying that the isolate produced 1-3 oc endo-gluconase.To study the effect of glucose and osmotic pressure on 0.1% glucose and 0.1% NaCl were augmented to CMC-contained media.Glucose augmentation affects cellulolytic activity of culture,probably due to higher biomass production in media. NaCl addition appear not to affect cellulolytic ability. Profile of pH also varied depend on the cultivation media. Maximum growth rate was achieved when glucose was added which was implying that glucose stimulate cell growth.
DIVERSITY AND ECOLOGICAL PERSPECTIVE OF SOIL YEAST IN GUNUNG HALIMUN NATIONAL PARK*[Khamir Tanah Taman Nasional Gunung Halimun: Keragaman dan Perspektif] Atit Kanti; I Made Sudiana
BERITA BIOLOGI Vol 6, No 1 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i1.1167

Abstract

Taman Nasional Gunung Halimun merupakan hutan hujan tropika yang berada di pulau Jawa. Penelitian ini ditujukan untuk mengetahui populasi dari khamir tanah pada berbagai ketinggian tempat (600 m, 1000 m, dan 1500 m) di atas permukaan laut, dan selanjutnya mempelajari peran ekologi dari khamir, dilakukan dengan mengisolasi dan menganalisa karakter fisiologi terutama dalam mendekomposisi selulosa dan hidrolisa fosfat. Khamir yang diisolasi dari tanah dan daerah perakaran dimurnikan dan ditumbuhkan pada media yang mengandung carboxymethylcellulose (CMC) sebagai sumber karbon utama, dan juga ditumbuhkan pada media pivoskaya untuk mengetahui kemampuan pelarutan Ca3(PO4)2. Populasi khamir pada daerah yang atas sedikit lebih tinggi .Sebanyak 23 isolat khamir yang termasuk dalam marga Debaryomyces, Pichia, Rhodotorula, dan Candida diisolasi dari tanah.15 isolat mempunyai kemampuan menghidrolisa selulosa dan 9 isolat mampu melarutkan Ca3(PO4)2. Khamir ditemukan di tanah dan daerah perakaran dan memegang peran penting dalam degradasi senyawa organik dan mineralisasi fosfat di dalam tanah.
CELLULOLYTIC YEAST ISOLATED FROM SOIL GUNUNG HALIMUN NATIONAL PARK* [Khamir Selulotik yang Diisolasi dari Tanah Taman Nasional Gunung Halimun] Atit Kanti; I Made Sudiana
BERITA BIOLOGI Vol 6, No 1 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i1.1173

Abstract

Beberapa khamir tanah yang lermasuk dalam marga Debaryomyces dan Candida diisolasi dari tanah Taman Nasional Gunung Halimun.Kemampuan selulotiknya diuji dcngan menumbuhkannya pada media yang mengandung carboxymethyl cellulose (CMC) sebagai sumber karbon utama. Pertumbuhan biomassa dan pH diikuti selama kultivasi. Pengamh penambahan glukosa terhadap aktivitas selulolitik juga dipelajari.Dari 23 isolat yang diuji, 19 termasuk khamir selulolitik, dan 1 isolat yang mempunyai kemampuan tertinggi dipelajari karakterisktik ezymatik sclulasenya. Penambahan glukosa menstimulasi pertumbuhan sel dan menyebabkan kebutuhan sel akan glukosa bertambah, dan sclanjutnya memacu sintesa ensim. Dari isolat-isolat yang diuji, isolat Debaryomyces S-6 mempunyai aktifitas paling tinggi yaitu sebesar 12 UI pada 96 jam inkubasi. Selama waktu kultivasi pH meningkat dari 6,5 menjadi 7. Adanya khamir yang menghidrolisa selulosa mengindikasikan khamir memegang peranan penting dalam transformasi bahan organik dalam tanah.
POTENCY OF RHIZOSPHERE BACTERIA TO PROMOTE RICE GROWTH UNDER SALINE CONDITION Sri Widawati; I Made Sudiana
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 23 No. 2 (2016)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (528.765 KB) | DOI: 10.11598/btb.2016.23.2.511

Abstract

Saline soil is a common problem in coastal paddy field, especially in Indonesia. Salinity affects rice growth and the activities of soil functional microbes, including functional bacteria, which play roles in plant growth. Some of these microbes are associated with rice plants and are able to survive under saline condition. The presence of functional microbes is also important to improve soil quality. Nitrogen and phosphate are essential soil nutrients and is available in soil due to the activities of nitrogen-fixing bacteria and free-living plant-associated bacteria.  The objective of the present study was to obtain nitrogen-fixing, phosphate solubilizing and Indole Acetic Acid  (IAA)-producing bacteria that are able to survive and promote the growth of rice under saline conditions. From rice and peanut rhizosphere, Ca-phosphate (Ca-P) solubilizing and nitrogen-fixing bacteria were isolated separately using specific media. Then, the Ca-P solubilizing ability, phosphomonoesterase activity and IAA-producing ability were quantitatively examined. Based on the abilities, 20 strains were selected and identified as Burkholderia cepacia-complex, Burkholderia anthina, Burkholderia cenocepacia, Bacillus cereus-complex (three strains), Achromobacter spanius, Azospirillum sp. (four strains), Azotobacter sp. (three strains), Rhizobium leguminosarum, Rhizobium sp. (two strains), and Pseudomonas sp. (three strains). The inoculation of several single strains or the mixture of the selected strains promoted the growth of rice under saline conditions. These inoculants could be potential as biofertilizer in saline paddy fields.
CHARACTERIZATION OF RHIZOBACTERIA ISOLATES FROM SOIL AND NODULES sri widawati; SULIASIH SULIASIH; Dwi Ningsih Susilowati; Yuki Muramatsu; I Made Sudiana
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 27 No. 3 (2020): BIOTROPIA Vol. 27 No. 3 December 2020
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (290.948 KB) | DOI: 10.11598/btb.0.0.0.1241

Abstract

The plant growth promoting rhizobacteria (PGPR) is a group of bacteria capable of colonizing plants roots, thereby developing a system and improving plants growth and yield. The objectives of the study is to characterize the PGPR activities of several bacterial isolates {in-vitro screening), to examine their activities in stimulating soybean growth (in-vivo screening), and to identify the bacterial species. These were isolated from nodules and soil samples collected from Mount Pancar in Bogor, West Java Province as well as from Bangkirai Hill and Wain River in East Kalimantan, Indonesia. The in-vitro PGPR activity characterization includes the N-fixing ability, ACC-deaminase, indole acetic acid (IAA) production, cellulolytic activity, P-solubilization, Phosphomonoesterase (PME-ase), and nifH-gene detection. The in-vivo PGPR activity with the greenhouse assay was conducted on soybean plant {Glycine max L.). All bacterial isolates were identified using molecular methods based on nucleotide sequence generated from 16S rRNA gene. Three isolates of soil and nodule bacteria with 7 characteristics of PGPR (N2 fixation, ACC-deaminase, cellulolytic activity, IAA production, solubilization index, P available, and PMEase activity) were successfully identified. These isolates were B045 {Klebsiella variicola InaCC B827), B116 (Klebsiella sp. InaCC B833), and B210 {Mangrovibacterplantisponsorlaa.CC B841). The greenhouse assay showed that the plant height, plant dry weight and number of flowers in soybean seedlings significantly increased with Bradyrhispbium sp. strain 4167, then with Klebsiella sp. InaCC B833 and Mangrovibacterplantisponsor InaCC B841. These bacterial isolates which were characterized and screened in-vitro for PGPR potentials and their representative isolates which were identified by 16S rRNA sequence analysis are key factors for selecting PGPR isolates to be commercialized later as bio-stimulant.
IDENTIFICATION OF YEASTS ISOLATED FROM GUNUNG HALIMUN NATIONAL PARK*[Identifikasi Khamir pada Taman Nasional Gunung Halimun] Atit Kanti; Heddy Julistiono; I Made Sudiana
BERITA BIOLOGI Vol 6, No 1 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i1.1177

Abstract

Dua puluh sembilan isolat khamir diisolasi dari tanah Taman Nasional Gunung Halimun. Sumber isolat berasal dari batang pohon lapuk, akar lapuk yang diambil dari Gunung Botol, Cikaniki, dan Cipta Rasa untuk dipelajari aspek taksonominya. Berdasarkan atas karakter morfologi dan fisiologi, isolat-isolat tersebut digolongkan kepada kelompok ascomyceteous, basidiomyceteous dan imperfect khamir. Selanjutnya ketiga golongan tersebut dimasukkan ke dalam sepuluh kelompok (Kelompok 1 sampai X). Dari 29 isolat tersebut, 7 isolat dimasukkan ke dalam kelompok I diindentifikasi sebagai Debaryomyces hansenii, 6 dalam kelompok II sebagai Candida sp, 2 dalam kelompok III sebagai Pichia membranafaciens, 5 isolat dalam kelompok IV sebagai Candida galacta, 1 dalam kelompok V sebagai Candida sake, 4 dalam kelompok VI sebagai Cryptococcus humicolus, 1 dalam kelompok VII sebagai Rhodotorula minuta, 1 dalam kelompok VIII sebagai Candida sp, 1 dalam kelompok Candida sp, dan 1 dalam kelompok X dalam Candida sp. Macam sampel tampaknya tidak berpengaruh kepada keragaman jenis khamir seperti ditunjukkan oleh jenis yang sama diisolasi dari berbagai jenis sampel. Dari banyaknya jenis khamir yang diisolasi menunjukkan bahwa keragaman jenis khamir di Taman Nasional Gunung Halimun tergolong tinggi.
Program Kewirausahaan Pembuatan Hiasan Penjor: Pemberdayaan Ibu PKK Berbasis Kearifan Lokal Ni Wayan Rustiarini; I Nyoman Kusuma Adnyana Mahaputra; I Made Sudiana; Ni Putu Nita Anggraini
Jurnal SOLMA Vol. 9 No. 2 (2020)
Publisher : Universitas Muhammadiyah Prof. DR. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/solma.v9i2.5520

Abstract

Pemerintah Indonesia telah memfokuskan perhatian pada upaya pengentasan kemiskinan di daerah pedesaan. Kegiatan pengabdian ini memprioritaskan pembangunan sumber daya manusia bernilai ekonomi melalui program kewirausahaan di Desa Kaliakah dan Desa Baluk. Terdapat beberapa permasalahan yang dihadapi, seperti 1) rendahnya pengetahuan masyarakat mengenai wirausaha, 2) kurangnya motivasi untuk melakukan wirausaha, serta 3) rendahnya kreativitas untuk memulai wirausaha. Bertitiktolak dari permasalahan tersebut, program kewirausahaan ini direalisasikan dalam bentuk penyuluhan dan pelatihan “mejejahitan” hiasan penjor. Program ini dilatarbelakangi pemikiran bahwa hiasan penjor merupakan kerajinan yang sering digunakan dalam kegiatan upacara agama dan adat di Bali. Hasil evaluasi kegiatan menunjukkan bahwa mayoritas peserta menyatakan pelatihan menarik dan bermanfaat. Selain itu, peserta juga mampu memahami materi yang diberikan sehingga memiliki keinginan untuk menjadi wirausaha, khususnya berkaitan dengan pembuatan hiasan penjor.
PERSOALAN ORTOGRAFI PENYERAPAN KOSAKATA SANSKERTA DALAM BAHASA INDONESIA I Made Sudiana
Kadera Bahasa Vol 2, No 2 (2011): Kadera Bahasa Vol.2 no.2
Publisher : Balai Bahasa Sulawesi Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6357.652 KB) | DOI: 10.47541/kaba.v2i2.54

Abstract

Sanskrit has a different writing system with the Indonesian language. Sanskrit uses Devanagari characters, while the Indonesian uses Latin script. Indonesian absorbs much vocabulary from other languages; one of them is from Sanskrit. Differences in sound system and writing system cause problems in absorption. The issue that arises is how to write words that absorbed it. The writing system in the absorption is often problematic when a language does not recognize the sound of the source language. Sanskrit recognizes certain sounds that do not exist in Indonesian. Differences writing system would also cause problems in the orthography. Orthographic problems in Sanskrit vocabulary absorption into the Indonesian language includes writing fricative sounds, retroflex, consonant aspire. labiodental /v/ and bilabial /w/, schwa (pepet), and anusuara.
KERENTANAN EKOLOGI DAN STRATEGI PENGHIDUPAN PERTANIAN MASYARAKAT DESA PERSAWAHAN TADAH HUJAN DI PANTURA INDRAMAYU Ali Yansyah Abdurrahim; Arya Hadi Dharmawan; Satyawan Sunito; I Made Sudiana
Jurnal Kependudukan Indonesia Vol 9, No 1 (2014)
Publisher : Research Centre for Population, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jki.v9i1.109

Abstract

Rain fed paddy village in Indramayu North Coasthasa very high ecological vulnerability. Location at the end of the irrigation and flood discharge channel makes this village every year drought in the dry season and floods in the rainy season. Later, the frequency and intensity increase as climate change. This condition is the pressure that shook the liveli hoods of rural communities. To maintain and continue their livelihood, households from various social strata living doing various activities that are grouped into three livelihood strategies, namely (1) agriculture, (2) non-agricultural livelihood diversification, and (3) migration. Livelihood strategies carried out by combining its livelihood assets and livelihood assets belonging to other households who successfully accessible through existing social institutions in rural communities. With a wide range of social institutions that, in general, every household in the village Karangmulya trying to generate sustainable livelihood out comes for the household. Income, well-being (welfare), adaptation of livelihoods, food security, and sustainability of natural resources resulting in a comprehensive and coKeywords: vulnerability, floods, droughts, livelihood strategies, sustainable livelihoods
Integrasi Kearifan Lokal Bali dalam Buku Ajar Sekolah Dasar I Made Sudiana; I Gede Sudirgayasa
Jurnal Kajian Bali (Journal of Bali Studies) Vol 5 No 1 (2015): AIR DAN PERUBAHAN SOSIAL DI BALI
Publisher : Pusat Kajian Bali Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (649.066 KB)

Abstract

AbstractIntegration of Balinese local wisdom required to maintainthe noble values of Balinese culture through early education.One of them is to do a primary school textbook developmentthat is integrated with the Balinese local wisdom as analternative. Therefore, this study was conducted to examinea wide range of Balinese local wisdom with regard toprimary school education. This is a development research.Initial data collection is done with the study of documents,interviews, and questionnaires. The elementary school isused as a source document and the respondent amountedto six elementary schools representing urban, suburban,and rural. Based on the preliminary data analysis, prepareda textbook draft. Assessment is done with a texbook draftfeasibility assessment instruments adopted from the bookassessment instruments by National Education StandardsAgency. Analysis of results of the assessment carried outby qualitative descriptive with the help of Microsoft OfficeExcel 2007. Based on the results of research and discussion,it can be concluded, that there are a lot of Balinese localwisdom with values that can be integrated in primary schooleducation. The results also show that the integrated Balineselocal wisdom textbook draft classified as good and feasibleto implement.