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All Journal Tropical Medicine Journal ASPIRATOR Kesmas: Jurnal Kesehatan Masyarakat Nasional SEL Jurnal Penelitian Kesehatan Kesmas Indonesia: Jurnal Ilmiah Kesehatan Masyarakat BALABA (JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA)

Dyah Widiastuti

Unknown Affiliation

Author-ID : 468114

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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SPOT SURVEI LEPTOSPIROSIS DI KECAMATAN NGEMPLAK DAN NOGOSARI, KABUPATEN BOYOLALI, PROVINSI JAWA TENGAH Nova Pramestuti; Bina Ikawati; Dyah Widiastuti
Kesmas Indonesia Vol 7 No 1 (2014): Jurnal Kesmas Indonesia
Publisher : Jurusan Kesehatan Masyarakat dan Fakultas Ilmu-Ilmu Kesehatan Universitas Jenderal Soedirman

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ABSTRACTIn 2013 there were three leptospirosis cases reported in Boyolali. This study aimed to describe the epidemiology of leptospirosis including cases overview aboutperson, place and time, transmission history, the trap success in catching mice and rats, and species of Leptospira infected rodent in Ngemplak and Nogosari district, Boyolali. This study was a cross sectional study. Rodent traping conducted at three locations with leptospirosis cases in Ngemplak and Nogosari District, Boyolali, in April 2013. A total of 300 traps wereinstalled, 2 traps inside and 2 traps outside for each house during three days. The trapped mice and rats were identified, counted their population density and their kidneys were taken for Leptospira examainationusingPCR (Polymerase Chain Reaction) assay. Data were processed and analyzed descriptively, presented in a frequency distribution form. The result showed that transmission of leptospirosis may occured around the case’ house, the river and rice fields. The highest rodentsuccess trapwas found in KismoyosoVillage (14 %). Rat species which were found consisted of R.tanezumi, and R. indica Bandicota argentiventer, there was no rat kidney samples infected with Leptospira bacteria according to PCR assay. Leptospirosis transmission in Boyolali should be noteddue to the high rat trap success and the location which was closed to the river . Key words: Survey,leptospirosis, rats Kesmasindo, Volume 7( 1 )Juli 2014, Hal 63-70

Mikroorganisme Patogen pada Feses Tikus Dyah Widiastuti; Nova Pramestuti; Endang Setiyani; Harjianti Fajar Rahayu
Jurnal Kesehatan Masyarakat Nasional Vol. 8 No. 4 November 2013
Publisher : Faculty of Public Health Universitas Indonesia

Tikus liar dan domestikasi mempunyai potensi faktor risiko bagi kesehatan masyarakat. Mereka dapat membawa mikroorganisme yang dapat ditransmisi melalui kontak dengan urine maupun feses atau melalui ektoparasit yang ada di tubuh mereka. Pada penelitian ini, diamati prevalensi mikroorganisme zoonotik pada tikus yang tertangkap di Pasar Kota Kabupaten Banjarnegara. Bakteri Salmonella paratyphi B dan Salmonella paratyphi C menunjukkan prevalensi yang paling tinggi (masing-masing 20%). Spesies bakteri lain yang ditemukan antara lain Salmonella typhimurium, Citrotobacter, Citrotobacter frendii, Enterotobacter cloacae, Escherichia coli (E. coli) dan Proteus miriabilis. Hasil penelitian ini mengindikasikan bahwa tikus yang berada di pasar dapat berperan sebagai reservoir untuk berbagai bakteri zoonotik seperti Salmonella dan E.coli.Commensal as well as wild rats and mice may present a potential risk to public health. They may harbour microorganisms that can be transmitted either through contact with infected rodent urine or faeces, or through ectoparasites. Prevalence of zoonotic microorganism in house rat (Rattus tanezumi) was studied in City Market of Banjarnegara Regency. Salmonella paratyphi B and Salmonella paratyphi C showed the highest prevalence in faecal material of Rattus tanezumi (20%). The other species of bacteria such as Salmonella typhimurium, Citrotobacter, Citrotobacter frendii, Enterotobacter cloacae, Eschericia coli (E. coli) and Proteus miriabilis were also found in this study. These results indicated that house rat may act as reservoir for zoonotic bacteria such as Salmonella and E.coli.

Variations in Dilution of DSSE 10 Antibody in Immunocytochemistry Technique to Detect Dengue-3 Virus in Aedes aegypti Mosquitoes Dyah Widiastuti; Sitti Rahmah Umniyati
Tropical Medicine Journal Vol 1, No 1 (2011): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/tmj.4566

Introduction: Dengue viruses, globally the most prevalent arboviruses, are transmitted to humans by persistently infected Aedes mosquitoes. The most important vector of Dengue virus is the mosquito Ae. aegypti, which should be the main target of surveillance and control activities. Virologic surveillance for Dengue viruses in its vector has been used as an early warning system to predict outbreaks. Detection of Dengue virus antigen in mosquito head squash using immunocytochemical streptavidin biotin peroxidase complex (SBPC) assay is an alternative method for Dengue vector surveillance.Objectives: The study was aimed to compare several variations of MAb DDSE10 dilutions used in immunocytochemical SBPC assay to detect Dengue virus infection in head squash of Ae. aegypti.Methods: The study design was experimental. Artifi cially-infected adult Ae. aegypti mosquitoes of DENV 3 were used as infectious samples and uninfected adult Ae. aegypti mosquitoes were used as normal ones. The immunocytochemical SBPC assay using monoclonal antibody DSSE10 with 4 variations of dilution (1:5, 1:10, 1:20, and 1:50) was applied on mosquito head squash to detect Dengue virus antigen. The results were analyzed descriptively.Results: All variants of MAb DSSE10 dilutions in immunocytochemical SBPC assay showed positive imunoreaction in infected mosquito head squash. All variants of MAb DSSE10 dilutions in immunocytochemicalSBPC assay showed negative immunoreaction in uninfected mosquito head squash.Conclusion: Monoclonal antibody DSSE10 could be used in immunochemistry technique to detect Dengue-3 virus antigen in Aedes aegypti infected intrathoracally, with 1:50 dilution.Keywords: Aedes aegypti, Dengue virus, Immunocytochemical, SPBC, Monoclonal Antibody DSSE-10

Temephos Resistance in Culex quinquefasciatus Population from Pabean Subdistrict Pekalongan Dyah Widiastuti; Sunaryo Sunaryo; Siwi Pramatama Mars Wijayanti
ASPIRATOR - Journal of Vector-borne Disease Studies Vol 11 No 2 (2019): Jurnal Aspirator Volume 11 Nomor 2 2019
Publisher : Loka Litbang Kesehatan Pangandaran

Abstrak. Pekalongan adalah satu di antara daerah dengan kejadian filariasis limfatik sejak beberapa tahunyang lalu dan Culex quinquefasciatus merupakan vektor utama penyakit ini di Pekalongan. Penelitian inibertujuan untuk mengetahui status kerentanan terhadap temephos dan menggambarkan aktivitas esterasepada populasi Cx. quinquefasciatus di Pekalongan. Penelitian dilakukan pada bulan April-Juli 2018. Ujikerentanan insektisida dilakukan pada larva tangkapan liar Cx. quinquefasciatus dari Pekalongan dengandosis temephos yang diskriminatif (0,02 ppm). Aktivitas Esterase larva ini diuji secara biokimia untukmempelajari mekanisme resistensi. Pada semua ulangan, angka kematian akibat temephos bervariasi dari70% sampai 75%, menunjukkan bahwa Cx. quinquefasciatus di tempat penelitian resisten terhadaptemephos. Hasil uji biokimia menunjukkan peningkatan aktivitas alfa dan beta esterase pada populasi Cx.quinquefasciatus. Hasil yang disajikan di sini memberikan laporan dan informasi dasar tentang statusresistensi terhadap temephos pada Cx. quinquefasciatus di Pekalongan. Hal ini seharusnya menjadiperhatian bagi dinas kesehatan dalam manajemen pengendalian vektor.

UJI ANTIMIKROBA EKSTRAK JAHE MERAH (ZINGIBER OFFICINALE) TERHADAP STAPHYLOCOCCUS AUREUS Dyah Widiastuti; Nova Pramestuti
Sel Jurnal Penelitian Kesehatan Vol 5 No 2 (2018): SEL Jurnal Penelitian Kesehatan
Publisher : Balai Penelitian dan Pengembangan Kesehatan Aceh

Infeksi Staphylococcus aureus menjadi masalah yang serius saat ini karena meningkatnya resistensi bakteri terhadap berbagai jenis antibiotik (Multi Drug Resistance/ MDR). Meluasnya resistensi bakteri terhadap obat-obatan yang ada, mendorong pentingnya upaya untuk menemukan langkah alternatif dengan pemberian obat-obatan pencegah penyakit infeksi dari bahan alam. Ekstrak jahe merah dikaji aktivitas anti mikroba terhadap S. aureus. Penelitian ini menggunakan uji coba kontrol yang sepenuhnya acak dengan lima ulangan. Dalam penelitian ini hanya terdiri dari satu faktor saja, yaitu pemberikan ekstrak jahe merah dengan menggunakan taraf/ level: 20%, 40%, 60%, 80% dan 100%. Hasil penelitian menunjukkan bahwa ekstrak jahe merah (Zingiber officinale var. Rubrum) pada konsentrasi 100% memberikan penghambatan tertinggi terhadap pertumbuhan S. aureus (12,54 ± 0,76 mm). Aktivitas antibakteri ekstrak rimpang jahe dikategorikan lemah dalam menghambat pertumbuhan S. aureus. Nowadays Staphylococcus aureus infection becomes a serious problem due to increased bacterial resistance to various types of antibiotics (Multi Drug Resistance/ MDR). Widespread bacterial resistance to existing drugs, prompting the importance of efforts to find alternative measures by administering medicines to prevent infection from natural substances. Red ginger extract was investigated for the antimicrobial activities against Staphylococcus aureus. This study used completely randommized control trial with three replications. The first factor was the tested microbe and the second was the fresh extract of the ginger rhizome. The result showed that fresh extract of red ginger (Zingiber officinale var. Rubrum) at concentration 100% gave the highest inhibition to the growth of S. aureus (12.54± mm). The antibacterial activity of ginger rhizome extract is categorized as weak in inhibiting the growth of S. aureus.

UJI EFIKASI EKSTRAK DAUN BABADOTAN SEBAGAI INSEKTISIDA NABATI TERHADAP LALAT RUMAH (Musca domestica) DI LABORATORIUM Dyah Widiastuti; Shinta Shinta
BALABA: JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA Edisi 007 Nomor 02/Tahun IV Desember 2008
Publisher : Balai Penelitian dan Pengembangan Kesehatan Banjarnegara Badan Litbangkes Kemenkes RI

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A study to evaluate the efficacy of babadotan leaves extract against adult Musca domestica was conducted in Loka Litbang P2B2 Banjarnegara Laboratory at Mei 2008. The study used 5 doses of extract (10,30, 50,70 dan 90%, as well as control treatment). Each doze used 3 replicates. The results showed that babadotan extract was not effective tocontrol more than 70% flies. A nova analysis showed that there was different count of death of flies between control and treated cages.

KEANEKARAGAMAN JENIS NYAMUK Anopheles DI DAERAH DENGAN ATAU TANPA KEBUN SALAK DI KABUPATEN BANJARNEGARA Dyah Widiastuti; Bambang Yunianto; Bina Ikawati
BALABA: JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA Edisi 002 Nomor 01/Tahun II Juni 2006
Publisher : Balai Penelitian dan Pengembangan Kesehatan Banjarnegara Badan Litbangkes Kemenkes RI

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Anopheles has been known as vectors of malaria and filaria. A research which was aimed to evaluate the diversity of Anopheles mosquitoes at Salak and Non Salak Areas was conducted. This Research located in Banjarnegara Regency which lay in a mountainous in the middle of Central Java (7'12"- 7'31 N and 109'20"-109'45"W). The location was divided into two groups i.e. (1) Kendaga Village (Banjarmangu Subdistrict) representing of Salak area, (2) Badakarya Village (Punggelan Subdistrict) representing Non Salak area. Mosquitoes were collected by landing and resting collection methods. All mosquitoes were anaesthetized with chloroform and identified under microscope. Shanon-Weaver Index and Eveness Index were measured to evaluate the diversity of Anopheles mosquitoes. The results showed there were 6 species of Anopheles from both areas i.e. A. aconitus. A. balabacensis. A. barbirostris. A. kochi. A. vagus and A. maculatus. Result of examination by Independent Sample T-Test indicated that the diversity index value between two areas were not significantly different.

LEPTOSPIROSIS PADA MANUSIA DI KABUPATEN BANYUMAS, PROVINSI JAWA TENGAH Bina Ikawati; Sunaryo Sunaryo; Dyah Widiastuti
BALABA: JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA Volume 9 Nomor 1 Juni 2013
Publisher : Balai Penelitian dan Pengembangan Kesehatan Banjarnegara Badan Litbangkes Kemenkes RI

ABSTRACT. Leptospirosis is one of infected diseases that caused by bacteria pathogen called Leptospira Sp,transmitted direct or indirect from infected animal to human, also known as zoonotic deseases. Indonesia includein middle category for leptospirosis incidence with 1-10 per 100.000 population.Leptospirosis in Central Java at2011 reported in Demak, Purworejo, Klaten, Pati, Wonogiri, Cilacap, Jepara District, Semarang District andSemarang City. Clinical leptospirosis in Banyumas District found but underreported. Aimed of this research wasto confirm leptospirosis at human in Banyumas district. Cross sectional design by case screening in 3 hospital and2 Primary Health Care at Juni-November 2012. Sera from patient examine by leptotec IgG IgM and MAT(Microscopic Aglutination Test), risk factor tracer had been done in patient from Banyumas. As much 25 clinicalleptospirosis examinated with laboratory test showed 6 serra positive by leptotec IgG IgM and with MAT(Microscopic Aglutination Test). MAT test showed 4 sera clinical leptospirosis that reactive with Leptospira Spand 1 negative but reactive at low titre 1:80. Risk factor of leptospirosis was flood and poor housing, bad hygienesanitation, bad waste management. Leptospirosis case confirmed in Banyumas

PENYELIDIKAN EPIDEMIOLODI MALARIA DI KECAMATAN PAGEDONGAN KABUPATEN BANJARNEGARA TAHUN 2006 Dyah Widiastuti; Tri Ramadhani; Asyhar Tunissea
BALABA: JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA Edisi 003 Nomor 02/Tahun II Desember 2006
Publisher : Balai Penelitian dan Pengembangan Kesehatan Banjarnegara Badan Litbangkes Kemenkes RI

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Kecamatan Pagedongan merupakan salah satu Kecamatan HCI pada tahun 2002 dan 2003, namun pada tahun 2004 mengalami penurunan, API mencapai 2,95%o tahun 2004 dan 0,4 %o pada tahun 2005. Akan tetapi pada bulan Pebruari 2006 di wilayah Puskesmas Pagedongan terjadi peningkatan kasus malaria berawal dari adanya kasus impor (luar Jawa). Dengan didukung oleh kemudahan transportasi dan komunikasi membuat malaria dapat berpindah serta tidak mengenal batas wilayah administrasi. Pada awalnya kasus malaria hanya terfokus pada Desa Gunung jati, akan tetapi hanya dalam waktu singkat penyakit ini sudah merambah ke Desa lain antara lain Pagedongan, Lebakwangi, Kebutuhduwur, Duren dan Gentansari. Untuk mendapatkan gambaran epidemiologi kejadian peningkatan malaria di Kecamatan Pagedongan, Tim Loka Litbang P2B2 Banjarnegara berkolaborasi dengan pihak Puskesmas Pagedongan dan Dinas Kesehatan Kabupaten Banjarnegara mengadakan kegiatan Penyelidikan Epidemiologi (PE) Malaria di Kecamatan tersebut.

Trichuris trichiura Endang Setiyani; Dyah Widiastuti
BALABA: JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA Edisi 007 Nomor 02/Tahun IV Desember 2008
Publisher : Balai Penelitian dan Pengembangan Kesehatan Banjarnegara Badan Litbangkes Kemenkes RI

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Trichuris trichiura

Co-Authors Anggun Paramita Djati Asyhar Tunissea Bambang Yunianto Bina Ikawati Endang Setiyani Harjianti Fajar Rahayu I Gusti Agung Komang Diafari Djuni Hartawan Nastiti Wijayanti Nova Pramestuti Nova Pramestuti Novia Tri Astuti Nur Ika Hariastuti Puji Astuti Shinta Shinta Sitti Rahmah Umniyati Siwi Pramatama Mars Wijayanti Siwi Pramatama Mars Wijayanti Sunaryo Sunaryo Sunaryo Sunaryo Tri Isnani Tri Ramadhani Tri Wijayanti

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