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Hubungan Ekspresi Matriks Metaloproteinase-9 dengan Jenis Histopatologi Karsinoma Serviks dan Invasi Limfovaskular Meta Oktora; Salmiah Agus; Pelsi Sulaini
Majalah Patologi Indonesia Vol 28 No 3 (2019): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

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ABSTRAKLatar belakangMetriks metaloproteinase 9 (MMP-9) merupakan enzim proteolitik yang diduga berperan penting dalamprogresivitas suatu keganasan khususnya pada serviks. Adanya invasi limfovaskular (LVI) dan ekspresi MMP-9yang tinggi akan menyebabkan proses degradasi jaringan serviks menjadi lebih cepat dan mempermudahproses metastasis. Jenis adenokarsinoma diduga memiliki prognostik yang buruk dibanding karsinoma selskuamosa (KSS). Penelitian ini bertujuan untuk melihat persentase ekspresi MMP-9 dan hubungannya denganjenis histopatologik karsinoma serviks dan invasi tumor limfovaskular.MetodePenelitian ini menggunakan metode analitik potong lintang. Sampel pada penelitian ini sebanyak 36 kasus yangterdiri atas 18 sampel KSS dan 18 sampel adenokarsinoma. Sampel diperoleh dari blok parafin yang berasaldari tindakan operasi atau biopsi di laboratorium Patologi Anatomik yang berada di Sumatera Barat. Sediaan direview dan dinilai invasi tumor limfovaskular. Pewarnaan imunohistokimia dilakukan dengan antibodi primerMMP-9, kemudian ekspresinya dinilai pada stroma dan sitoplasma sel.HasilEkspresi MMP-9 ditemukan positif sebanyak 80,6% pada semua sampel, di mana 41,1% pada KSS dan 58,6%pada adenokarsinoma serviks. Rerata ekspresi MMP-9 pada KSS 17,4 dan pada adenokarsinoma 19,56.Ekspresi MMP-9 positif pada semua sampel dengan LVI.KesimpulanTidak terdapat hubungan ekspresi MMP-9 dengan jenis histopatologi karsinoma serviks (p=0,088) dan LVI(p=0,566). Rata-rata ekspresi MMP-9 tinggi pada jenis adenokarsinoma, tetapi secara statistik tidak terdapatperbedaan yang bermakna (p=0,563).

Hubungan Ekspresi Vascular Endothelial Growth Factor (VEGF) dengan Derajat Diferensiasi dan Invasi Limfovaskular pada Karsinoma Urotelial Infiltratif Kandung Kemih Dini Andri Utami; Salmiah Agus; Yenita; Husna Yetti
Majalah Patologi Indonesia Vol 31 No 2 (2022): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

Background Urothelial carcinoma is the most common malignancy in the bladder with an incidence up to 95% and 70-85% of them are bladder infiltrating urothelial carcinoma. To improve the management of bladder urothelial carcinoma, prognostic factors are required. One such factor is angiogenesis, which affects growth, development and metastasis. Vascular endothelial growth factor (VEGF) is the main pro-angiogenic factor to control angiogenesis. Expression of VEGF is correlated with progression of bladder infiltrating urothelial carcinoma, such as grade and lymphovascular invasion. The aim of this study was to determine the correlation of VEGF expression with grade of differentiation and lymphovascular invasion in bladder infiltrating urothelial carcinoma. Methods This research was a retrospective observational cross sectional study with 48 cases of bladder infiltrating urothelial carcinoma in four Anatomical Pathology Laboratory in West Sumatera 2018 and 2019. Samples were reevaluated of HE slide for grade of differentiation and lymphovascular invasion. VEGF expressions in tumor cell s were analyzed using immunohistochemistry staining. Bivariate statistical analysis used Fisher's Exact test and value p<0.05 was considered significant. Results Bladder infiltrating urothelial carcinoma high grade had more positive VEGF expression (91.7%), while low grade had more negative VEGF expression (58.3%). Lymphovascular invasion positive was mostly found with positive VEGF expression (75%). Statistical analysis showed significant correlation between VEGF expression with grade of differentiation (p=0.001) and lymphovascular invasion (p=0.004). Conclusion The conclusion was VEGF expression had significant correlation with grade of differentiation and lymphovascular invasion of bladder infiltrating urothelial carcinoma.

Pengaruh Cairan Cultur Filtrate Fibroblast (CFF) Terhadap Penyembuhan Luka; Penelitian eksperimental pada Rattus Norvegicus Galur Wistar Oky Masir; Menkher Manjas; Andani Eka Putra; Salmiah Agus
Jurnal Kesehatan Andalas Vol 1, No 3 (2012)
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v1i3.78

AbstrakLatar belakang:Metode penyembuhan luka telah mengalami perkembangan, baik berupa suatu produk atau stimulan terhadap proses biologis tubuh dalam menkompensasi luka. Fibroblas merupakan salah satu komponen penyembuhan yang berperan penting dalam proses fibroplasia. Culture Filtrate Fibroblast (CFF) merupakan hasil kultur fibroblas yang akan dibuktikan efeknya terhadap proses percepatan penyembuhan luka pada penelitian ini. Metode. Penelitian ini menggunakan desain eksperimental dengan metode post test only control group design dan rancangan acak kelompok (RAK) dengan menggunakan tikus putih wistar. Hewan coba dibagi menjadi 4 kelompok, yaitu 2 kelompok perlakuan yang diberikan CFF ke area eksisi luka dan kelompok kontrol yang diberikan larutan NaCl 0,9% ke area eksisi luka. Data diolah dengan SPSS 16.0. Data Kategori dianalisa dengan Chi-squared dan data numerik dengan Independent T-test. Hasil. Dari tingkat penyembuhan tidak ditemukan perbedaan pada kedua kelompok, namun perubahan restriksi jaringan lebih besar pada kelompok perlakuan. Pada skor pembentukan kolagen, derajat epitelisasi serta jumlah pembentukan pembuluh darah baru pada hari ke-3 tidak ditemukan perbedaan antara kedua kelompok. Namun pada pengamatan hari ke-7 memperlihatkan pembentukan kolagen, derajat epitelisasi serta jumlah pembentukan pembuluh darah baru lebih banyak pada kelompok perlakuan. Pada fibrosis hari ke-3 dan hari ke-7 memperlihatkan terjadinya fibrosis lebih banyak pada kelompok perlakuan dibanding kontrol. Pada pengamatan terjadinya infeksi hari ke-3 memperlihatkan infeksi lebih sedikit pada kelompok perlakuan dan terjadinya infeksi sama pada hari ke-7. Kesimpulan. CFF memberikan tingkat penyembuhan luka yang lebih baik dibanding NaCl.Kata kunci: CFF, NaCl 0,9 %, tingkat penyembuhan luka.Abstract Background: Wound healing methods have been developed, either a product or a stimulant to the body's biological processes in wound compensation. Fibroblasts is one component that plays an important role in the healing process of fibroplasia. Culture filtrat Fibroblast (CFF) is a result of fibroblast culture to be proven effect on the acceleration of wound healing in this study.Methods. This study used an experimental design method post test only control group design and randomized block design (RBD) by using wistar mice. Experimental animals were divided into 4 groups, the two groups of treatment given to the area of excision wound CFF and the control group were given 0.9% NaCl solution to the excision wound area. Data processed with SPSS 16.0. Data were analyzed with categories Chi squared and numerical data by the Independent T-test.Result. From degree of wound healing is not found differences in both groups, but the changes in the network restriction greater in the treatment group. The score formation of collagen, the degree of epithelialization and the amount of neovascularisation formation at 3rd day there was no difference between the two groups. However, the observation of 7th day shows the formation of collagen, the degree of epithelialization and the amount of neovascularisation formation more in the treatment group. On the 3rd day fibrosis and 7th day showed more fibrosis in the treatment group compared to controls. In observation of the 3rd day infection showed fewer infections in the treatment group and the same infection between the two group at 7th day.Conclusion. CFF give wound healing better than NaCl.Keywords:CFF, NaCl 0,9 %, degree of wound healing.

Efek Pemberian Suntikan Subkutan Vitamin C Terhadap Luka Insisi Dermal Surya Darma; Menkher Manjas; Deddy Saputra; Salmiah Agus; Erkadius .
Jurnal Kesehatan Andalas Vol 2, No 3 (2013)
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v2i3.247

Abstrak Vitamin C berfungsi sebagai kofaktor enzyme prolil dan lysil hydroxilase. Enzym tersebut berfungsi dalam proses hidroksilasi yang membentuk ikatan hidroksiprolin dan hidroksilisin pada fibroblast dalam membentuk kolagen. Selain itu Vitaimin C juga berfungsi meregulasi dan menstabilkan trankripsi gen mRNA prokolagen pada proses pembentukan kolagen di dermis. Berdasarkan hal tersebut diatas, peneliti tertarik untuk membuktikan apakah pemberian vitamin C subkutan disekitar luka insisi dermal berefek pada pembentukan kolagen yang lebih padat dalam proses penyembuhan luka. Metode: Penelitian eksperimental ini menggunakan tikus Wistar sebanyak 32 ekor, yang dibagi menjadi 2 kelompok yaitu 16 ekor sebagai kontrol dan 16 ekor lagi sebagai perlakuan. Pada kedua kelompok dilakukan insisi di punggung sepanjang 2 cm. Kelompok perlakuan diberi suntikan vitamin C subkutan disekitar luka insisi dermal sebanyak 9 mg (0,09ml), sedangkan kelompokkontrol tidak diberikan.Pada hari kelima dilakukan pengambilan jaringan luka pada kedua sampel untuk pemeriksaan kepadatan kolagen secara mikroskopik. Hasil:Kepadatan kolagen pada hari kelimamenunjukkan perbedaan yang bermakna dari efek penyuntikan vitamin C subkutan terhadap kepadatan kolagen (χ2 = 5,833; P

Efek Pemberian Suntikan Subkutan Vitamin C Terhadap Luka Insisi Dermal Surya Darma; Menker Manjas; Deddy Saputra; Salmiah Agus; Erkadius Erkadius
Jurnal Kesehatan Andalas Vol 3, No 3 (2014)
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v3i3.161

Abstrak Vitamin C berfungsi sebagai kofaktor enzyme prolil dan lysil hydroxilase. Enzym tersebut berfungsi dalam proses hidroksilasi yang membentuk ikatan hidroksiprolin dan hidroksilisin pada fibroblast dalam membentuk kolagen. Selain itu Vitaimin C juga berfungsi meregulasi dan menstabilkan trankripsi gen mRNA prokolagen pada proses pembentukan kolagen di dermis. Berdasarkan hal tersebut diatas, peneliti tertarik untuk membuktikan apakah pemberian vitamin C subkutan disekitar luka insisi dermal berefek pada pembentukan kolagen yang lebih padat dalam proses penyembuhan luka. Metode: Penelitian eksperimental ini menggunakan tikus Wistar sebanyak 32 ekor, yang dibagi menjadi 2 kelompok yaitu 16 ekor sebagai kontrol dan 16 ekor lagi sebagai perlakuan. Pada kedua kelompok dilakukan insisi di punggung sepanjang 2 cm. Kelompok perlakuan diberi suntikan vitamin C subkutan disekitar luka insisi dermal sebanyak 9 mg (0,09ml), sedangkan kelompokkontrol tidak diberikan.Pada hari kelima dilakukan pengambilan jaringan luka pada kedua sampel untuk pemeriksaan kepadatan kolagen secara mikroskopik. Hasil:Kepadatan kolagen pada hari kelimamenunjukkan perbedaan yang bermakna dari efek penyuntikan vitamin C subkutan terhadap kepadatan kolagen (χ2 = 5,833; P<0,05). Kesimpulan: Penyuntikan vitamin C subkutan disekitar luka insisi dermal efektif dalam meeningkatan kepadatan kolagen. Kata kunci: suntikan vitamin C subkutan, kepadatan kolagen.Abstract Vitamin C functions as enzyme co-factor for prolyl and hidroxylase lysil. The enzyme functions in hydroxylase process that builds hydroxyproline and hydroxylysine bondsin fibroblast in the synthesis of collagen. Besides that, vitamin C also functions in regulating and stabilizing procollagen mRNA gen transcription in dermal collagen synthesis. Based on the facts above, researchers are interested to prove whether subcutaneous injection of vitamin C around dermal insisional wound would result in more compact collagen synthesis in wound healing. Method:This experimental study used32 Wistar rats, divided into two group that is 16 rats as control and 16rats as experimental group. All groups underwent 2 cm long incision at the back. Experimental group were given 9mg (0.09ml) subcutaneous injection of vitamin c around the wound, while the control group were not. On the fifth day, wound tissue are taken on both sample to check the collagen density microscopically. Result:Collagen density on the fifth day showed significant difference between the two groups(χ2 = 5,833; P<0,05). Discussion:Subcutaneous vitamin C injection around the dermal incision wound is effective in increasing collagen density. Keywords: subcutaneous vitamin C injection,collagen density

PENERAPAN TEKNIK PERIODIC ACID SCHIFF (PAS) PADA DIAGNOSIS HISTOPATOLOGI Fajriza Yona; Salmiah Agus; Tofrizal Tofrizal
Collaborative Medical Journal Vol 5 No 1 (2022): Januari
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar

Histopathological tissue examination with routine staining sometimes cannot provide a clear morphology of certain cells or tissues, therefore special staining techniques based on histochemical reactions are needed. Periodic Acid-Schiff's (PAS) is a histochemical staining method specifically used to detect the presence of polysaccharide carbohydrates, neutral mucins and other glycoproteins. This article was written with the aim of increasing knowledge for practitionersor clinicians regarding the PAS technique procedure and how it is used in diagnosis. The method of writing scientific papers is a literature review sourced from various journals, books and scientific articles that are relevant and in accordance with the topics discussed. The PAS technique procedure begins with fixation in 10% neutral buffer formalin, preparation of materials and reagents, PAS staining with counterstain and interpretation. The addition of diastase was performed before PAS stain for PAS/D, and Alcian Blue staining was performed before PAS stain for the AB/PAS technique. Errors at each stage will affect the results of PAS staining. The use of combined PAS and PAS techniques in pathological diagnosis is to exclude the differential diagnosis of various tumors containing glycogen and mucin, examine the thickness of the basement membrane in various diseases, and identify Jamur and amoeba in tissue infections.

Hubungan Ekspresi Vascular Endothelial Growth Factor (VEGF) dengan Derajat Diferensiasi dan Invasi Limfovaskular pada Karsinoma Urotelial Infiltratif Kandung Kemih Dini Andri Utami; Salmiah Agus; Yenita; Husna Yetti
Majalah Patologi Indonesia Vol. 31 No. 2 (2022): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55816/mpi.v31i2.499

Background Urothelial carcinoma is the most common malignancy in the bladder with an incidence up to 95% and 70-85% of them are bladder infiltrating urothelial carcinoma. To improve the management of bladder urothelial carcinoma, prognostic factors are required. One such factor is angiogenesis, which affects growth, development and metastasis. Vascular endothelial growth factor (VEGF) is the main pro-angiogenic factor to control angiogenesis. Expression of VEGF is correlated with progression of bladder infiltrating urothelial carcinoma, such as grade and lymphovascular invasion. The aim of this study was to determine the correlation of VEGF expression with grade of differentiation and lymphovascular invasion in bladder infiltrating urothelial carcinoma. Methods This research was a retrospective observational cross sectional study with 48 cases of bladder infiltrating urothelial carcinoma in four Anatomical Pathology Laboratory in West Sumatera 2018 and 2019. Samples were reevaluated of HE slide for grade of differentiation and lymphovascular invasion. VEGF expressions in tumor cell s were analyzed using immunohistochemistry staining. Bivariate statistical analysis used Fisher's Exact test and value p<0.05 was considered significant. Results Bladder infiltrating urothelial carcinoma high grade had more positive VEGF expression (91.7%), while low grade had more negative VEGF expression (58.3%). Lymphovascular invasion positive was mostly found with positive VEGF expression (75%). Statistical analysis showed significant correlation between VEGF expression with grade of differentiation (p=0.001) and lymphovascular invasion (p=0.004). Conclusion The conclusion was VEGF expression had significant correlation with grade of differentiation and lymphovascular invasion of bladder infiltrating urothelial carcinoma.

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