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Journal : Indonesian Biodiversity Journal

ANTAGONIST, ANTIBACTERIAL ACTIVITY OF Stapylocossus aureus AND ISOLATE OF ORAL BACTERIA FROM THE ENDOGENOUS FUNGUS Apis dorsata BINGHAMI NEST Dina Rombot; Ellen Adil; Mario Walean; Virjinia Bastian
Indonesian Biodiversity Journal Vol. 2 No. 2 (2021): Agustus 2021
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v2i2.4934

Abstract

Apis dorsata Binghami is a honey bee endemic to Indonesia, living naturally in the forests of Sulawesi. This study aimed to obtain isolates and characteristics of endogenous fungi antibiotic activity from Apis dorsata Binghami nest. The study consisted of isolation of fungi from beehives using potato dextro agar, pure culture of fungi, antagonist test and antibiotic test using disc diffusion method. Antibiotic test was performed on oral bacteria and Staphylococcus aureus. The results obtained six fungal isolates from Apis dorsata Binghami's nest, namely isolates FAB1, FAB2, FAB3, FAB4, FAB5 and FAB6. The results of the antagonist test showed that the isolates FAB1, FAB2, FAB3, FAB4 and FAB5 were antagonistic. The results of the antibacterial test showed that all isolates produced an average bacterial growth inhibition of 17-18 mm on Staphylococcus aureus and isolates of oral bacteria. The inhibition zone lasts up to 3 x 24 hours so that the activity of the bacteria is bactericidal. From the results of this study, it can be concluded that the endogenous fungus Apis dorsata Binghami is a potential source of antibacterial bioactives.
PHYTOCHEMICAL ANALYSIS OF BITUNG TREE SEEDS (Baringtonia asiatica L.) Hendra Pratama Maliangkay; Rolef Rumondor; Mario Walean
Indonesian Biodiversity Journal Vol. 2 No. 3 (2021): Desember 2021
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v2i3.4989

Abstract

This study aims to obtain data on the phytochemical content of the methanol extract of Baringtonia asiatica. Obtain data on the phytochemical content of the B. asiatica clorform extract. This research was carried out in the Biology laboratory and Chemistry Laboratory, State University of Manado. The study consisted of extraction and phytochemical analysis using the Harborne method, the results showed that from the extraction process the highest yield was obtained in extracts with n-hexane solvent, then chloroform and methanol. The results of phytochemical analysis show that saponins are the phytochemical group with the strongest intensity found compared to other phytochemical groups. Furthermore, flavonoids were found in high intensity also in the analyzed samples.