Iswari S Dewi
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. (0251) 8337975

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KONSERVASI PAMELO {Citrus maxima (Burm.) Merr.} DENGAN PENURUNAN KONSENTRASI MEDIUM DAN SUKROSA Tyas, Kartika Ning; Susanto, Slamet; Dewi, Iswari S; Khumaida, Nurul
Buletin Kebun Raya Vol 15, No 2 (2012): Buletin Kebun Raya Vol. 15 (2) Juli 2012
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

Indonesia has high variety of pummelo [Citrus maxima (Burm.) Merr.], but only several cultivar are cultivated extensively. Others became vurnerable and the  germplasm has to be conserved to prevent their extinction. One of the conservation method is in vitro conservation  using a slow growth technique. Factorial experiment was used in designing the experiment, the first factor was MS medium concentration, i.e. 1/2MS and MS. The second factor was sucrose concentration, i.e. 0; 1; 2; and 3%. The results showed that low concentration of MS medium and sucrose reducing the leaf number and shoot length but increasing the root number and length.  Based on inhibition of growth,  the most reducing growth was planlet on MS without sucrose.
KONSERVASI PAMELO {Citrus maxima (Burm.) Merr.} DENGAN PENURUNAN KONSENTRASI MEDIUM DAN SUKROSA Tyas, Kartika Ning; Susanto, Slamet; Dewi, Iswari S; Khumaida, Nurul
Buletin Kebun Raya Vol 15, No 2 (2012): Buletin Kebun Raya Vol. 15 (2) July 2012
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Indonesia has high variety of pummelo [Citrus maxima (Burm.) Merr.], but only several cultivar are cultivated extensively. Others became vurnerable and the  germplasm has to be conserved to prevent their extinction. One of the conservation method is in vitro conservation  using a slow growth technique. Factorial experiment was used in designing the experiment, the first factor was MS medium concentration, i.e. 1/2MS and MS. The second factor was sucrose concentration, i.e. 0; 1; 2; and 3%. The results showed that low concentration of MS medium and sucrose reducing the leaf number and shoot length but increasing the root number and length.  Based on inhibition of growth,  the most reducing growth was planlet on MS without sucrose.
REGENERASI TUNAS DARI KALUS YANG TELAH DIBERI PERLAKUAN IRADIASI PADA PADI VARIETAS FATMAWATI [Shoot Regeneration of the Fatmawati Rice Variant Radiated Callie] Yunita, Rossa; Lestari, Endang G; Dewi, Iswari S
BERITA BIOLOGI Vol 11, No 3 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (154.311 KB) | DOI: 10.14203/beritabiologi.v11i3.506

Abstract

Gamma ray mutative induction for increasing genetic variation has been applied for plant prime variety engineering. The materials are derrived from seed organ, shoot and calli. Calli is a group of actively dividing cell and have not been organized to form plant. The benefit of using calli explant is that the gamma ray could directly shot to DNA in the nuclear cell in such a way that there is higher opportunity for genetic change to occur. The problema of using calli explant are the difficulties in regenerating the calli into shoots, due to the deformation as a result of radiation process. Therefore, this research is aimed at obtaining the appropriate media formulation for shoot regeneration from Fatmawati-rice calli which has been irradiated with gamma ray. The reseach was conducted in BB-Biogen laboratory consisting of three experiments, such as : (1) calli iradiation with the dosage of 0; 5; 10; 15; 20; 25; 30; 35; 40; 45; 50; 55 and 60 Gy, (2) shoot regeneration at the MS + BA (0, 1, dan 3 mg/l) + IAA (0 dan 0,8 mg/l) media, and BA (0, 1, and 3 mg/l) + zeatin ( 0; 0,1; 0,2 and 0,3 mg/l) + IAA 0,8 mg/l and (3) Shoot induction at MS + IBA (0, 1, 2 and 3 mg/l) media. The result shows that the range of LD50 was obtained at the dosage of 30 Gy, the most apropriate media for shoot regeneration is MS + BA 3 mg/l + IAA 0,8 + zeatin 0,1 mg/l and media for root induction is IBA 1 mg/l.