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International Journal of Biosciences and Biotechnology Vol 6 No 1 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (212.165 KB) | DOI: 10.24843/IJBB.2018.v06.i01.p01


To date, there are very few studies have been carried out on the effect of parasite infection on Bali cattle, and more importantly, there is no published data on the epidemiology of worm infections on Bali cattle in the island of Sumbawa, Indonesia. This research aimed to undertake a preliminary study on gastrointestinal (GI) parasitic infection on Bali cattle raised under semi-intensive farming system in five different sites in district of Dompu, Sumbawa island. Fecal samples of 76 Bali cattle, from three farmer groups namely site number 1, 4 and 5, were collected, processed and examined under a microscope, with 100x magnification, for egg fecal counting or oocyst fecal counting using the modified McMaster technique. The number of eggs/oocysts observed was then determined and identified. The results show that the eggs/oocysts ranged from 50-14800. The types of GI parasites identified were Strongyloides sp, Strongyle sp, Toxocara sp, Trichuris sp, and Coccidia. Coccidia oocyst is a type of parasite which was found with the highest percentage of 23.68% followed by Strongyle sp. (18.42%) and Toxocara sp. (10.52%). The overall prevalence of GI parasitic infection in cattle accounted for 43.42%. Young calves (0-3 months old) was more susceptible to GI parasitic infection among other age groups. This preliminary data could be used as a basic data on gastrointestinal parasitic infection in Dompu district. These results may also indicate the need to develop control strategies aiming at lowering the gastrointestinal parasitism.
Potensi Air Liur Sebagai Perantara dalam Pemeriksaan Noninvasive pada Hewan Piaraan (POTENTIAL OF SALIVA AS A MEDIATOR FOR THE NONINVASIVE EXAMINATION OF DOMESTIC ANIMALS) Depamede, Sulaiman Ngongu; Rosyidi, Anwar; Sriasih, Made; ., Dahlanuddin; Yulianti, Enny; ., Suparman
Jurnal Veteriner Vol 15 No 4 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Saliva as unique biological components of the oral cavity has potential as a mediator for noninvasivebiological test. For future livestock industry the application of noninvasive test is strongly necessary dueto animal welfare porpuse. Until now the research and development of the using saliva as a mediator forinvasive test is predominant for humans, while for livestock or domestic animals is still limited. Techniquesand methods that are commonly used are related to proteomics analysis. With this method a lot of thingsthat can be brought about proteomics of saliva are beneficial to the development of saliva-based biomarkers.Although this method is expensive, researches on the use of saliva in the field of livestock industries areurgently needed. It is expected that noninvasive biological test methods based on saliva as a mediator canbe performed immediately.
Studi Pendahuluan Cemaran Air Limbah Rumah Potong Hewan di Kota Mataram Aini, Aini; Sriasih, Made; Kisworo, Djoko
Jurnal Ilmu Lingkungan Vol 15, No 1 (2017): April 2017
Publisher : School of Postgraduate Studies, Diponegoro Univer

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (772.078 KB) | DOI: 10.14710/jil.15.1.42-48


ABSTRAKTujuan penelitian ini adalah untuk mengetahui cemaran baku mutu air limbah Rumah Potong Hewan (RPH) Sapi dan Ayam di Kota Mataram. Semua hasil melebihi baku mutu kecuali nilai pH. Kandungan ALT rerata air limbah RPH sapi 284004 x 104 koloni/ml dan mengandung mikroba jenis E.coli dan Salmonella. Sedangkan air limbah RPH ayam ALT rerata 101596 x 105 koloni/ml dan mengandung kuman jenis E. coli, Pseudomonas aeruginosa dan Klebsiella pneumoniae. Tingginya kandungan BOD, COD, TSS, ammonia, pH, minyak dan lemak maupun cemaran mikroba diduga disebabkan tidak difungsikannya Instalasi Pengolahan Limbah (IPAL) pada RPH sapi dan tidak optimalnya fungsi IPAL di RPH ayam.Kata kunci: RPH sapi, RPH ayam, Baku mutu limbah RPH, Cemaran mikroba, kota Mataram yABSTRACTThe purpose of this study was to determine the contamination of waste water quality standard and microbiologic contamination ofcattle and chicken slaughterhousein Mataram city. TPC average content of waste water of cattle slaughterhouse was 284004 x 104 colonies/ ml, and contained microbial species of E. coli and Salmonella. However, the TPC avarange of wastewater of chicken slaughterhouse accounted for 101596 x 105 colonies/ml and contained microbial species of E. coli, Pseudomonas aeruginosa and Klebsiella pneumoniae. High content of BOD, COD, TSS, ammonia, pH, oil and fats as well as microbial contamination may relate to disfunctionality of Waste Water Treatment Installation (IPAL) in cattle slaughterhouse and too less optimum function of the IPAL in chicken slaughterhouse.Keywords: cattle slaughter house, chicken slaughter house, slaughterhouse waste quality standard, microbial contamination, Mataram cityCitation: Aini, A., Sriasih, M, dan Kisworo, D. (2017). Studi Pendahuluan Cemaran Air Limbah Rumah Potong Hewan di Kota Mataram. Jurnal Ilmu Lingkungan, 15(1), 42-48, doi:10.14710/jil.15.1.42-48
Improvements of PCR Amplification of Guanine plus Cytosine-Rich Constructs of Mycobacterium tuberculosis Gene using DMSO Sabrina, Yunita; Lestarini, Ima Arum; Ekawanti, Ardiana; Sriasih, Made; Depamede, Sulaiman N.; Ali, Muhamad
Jurnal Kedokteran Vol 1 No 1 (2012)
Publisher : Faculty of Medicine Universitas Mataram

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Vaccine research entered a new era when several useful molecular research tools were established. Instead of attenuated virulent microorganisms or killed virulent microorganisms, effective subunit vaccines were developed using recombinant DNA technology. By using the technology, selected genes of the virulent microorganisms can be amplified, cloned, expressed, and evaluated as vaccine components in challenge studies. However, a major bottleneck with the amplification of functional genes from Mycobacterium tubeculosis containing guanine plus cytosin-rich templates is often hampered by the formation of secondary structures like hairpins and higher melting temperatures. To solve this problem in this research, the amplification reaction was modified by addition of dimethyl sulfoxide (DMSO) into amplification reaction mixtures. It was found that 10% (v/v) of DMSO in the reaction mixture improved the amplification of GC-rich template of M. tuberculosis gene. This result indicating that amplification of unbalanced content of G and C deoxyribonucleotides genome could be improved using low-cost organic molecule, DMSO. Therefore, the DMSO should be widely useful as an enhancer to improve the amplification of GC rich construct from other genome. Keywords: Mycobacterium tubeculosis,vaccine, dimethyl sulfoxide, Guanine-Cytosine
Ekstrak Daun Jati (Tectona grandis) Alternatif Pewarna pada Penghitungan Jumlah dan Viabilitas Sel Kultur Dibandingkan dengan Pewarna Tryphan Blue Sri Andayani, I Gusti Ayu; Sulastri, Sri; Hananto, Dwi Ampera; Sriasih, Made
Bioscientist : Jurnal Ilmiah Biologi Vol 8, No 2 (2020): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bjib.v8i2.3015


Tryphan blue comes from toluidine which is one of the bases of toluene isomer which has a natural pigment content of anthsiasin. Tryphan blue is commonly used for cell counting and for the calculation of tissue viability in laboratory mice. Basically the use of tryphan blue is dangerous for laboratory workers because it has a carcinogenic effect, mutagenesis, so other materials are needed especially safer and harmless natural materials. This study aims to compare the quality of teak leaf dye extract as a natural dye on the calculation of the number and viability of culture cells compared to tryphan blue dye. The study used the Complete Randomized Design (RAL) method with three treatment factors (96% ethanol, 14% citric acid, and Phospat Buffer Saline (PBS)) and observations of 5 replays be used for the calculation of the number and viability of spleen cells in the 1st, 10th, 20th, and 30th minutes with tryphan blue as a positive control. The results showed that the quality of teak leaf dye with ethanol solvent 96% viability does not differ materially, provides good color quality and obtained the same viability time as tryphan blue.
Pemeriksaan Kesehatan untuk Deteksi dan Pengelolaan Diabetes Mellitus dan Hiperkolestrol Aini, Aini; Nuratikah, Nuratikah; Ustiawaty, Jumari; Sriasih, Made
Jurnal Pengabdian Magister Pendidikan IPA Vol 4 No 2 (2021)
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v4i2.670


Diabetes Melitus (DM) merupakan penyakit yang berhubungan dengan sindrom yang memiliki ciri meningkatnya kadar glukosa darah atau dikenal dengan istilah hiperglikemia.Kondisi hiperglikemia ini dapat terjadi akibat gangguan sekresi, kerja insulin, atau oleh keduanya. Penyakit kronis seperti DM sangat rentan terhadap gangguan fungsi  yang bisa menyebabkan kegagalan padaorgan mata,ginjal,saraf, jantung dan pembuluh darah. Kolestrol adalah suatu molekul  atau komponen lemak di dalam sel. Lemak merupakan slah satu zat gizi yang sangat diperlukan oleh tubuh.lemak merupakan sumber energi yang memberikan kalori paling tinggi tetapi bila  terdapat kadar kolestrol yang tinggi (hiperkolestrol) dapat menimbulkan resiko yang tinggi. Pemeriksaan kesehatan (screning) kesehatan) belum menjadi kebiasaan masayakat pada umumnya, hal ini disebabkan karena kurangnya pengetahuan masyakat tentang screening kesehatan ataupun penyakit degeneratif dan kurangnya biaya dalam masyakat untuk melakukan pemeriksaan kesehatan secara rutin. Dapat disimpulkan bahwa pada  saat pemeriksaan   dilakukan di Bawaq Bageq Utara   kelurahan Dasan Agung kecamatan Selaparang  kota Mataram Jumlah responden yang datang sebanyak 37 orang warga.  Hasil pemeriksaan diperoleh  hasil warga yang hipertensi 56,7%     (21 orang) dan 43.2% (16 orang ) mempunyai kadar kolesterol di atas normal dan membutuhkan pengobatan.
Sosialisasi Perilaku Hidup Bersih dan Sehat (PHBS) di Tempat Kerja Sebagai Upaya Pencegahan Penularan Covid 19 Aini, Aini; Sriasih, Made
Jurnal Pengabdian Magister Pendidikan IPA Vol 3 No 2 (2020): .
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v3i2.466


Pemerintah provinsi NTB pada bulan Maret 2020 menyatakan bahwa penyebaran virus Corona telah sampai ke wilayah NTB. Salah satu upaya dalam pencegahan penyebaran virus Corona masuk ke dalam tubuh kita adalah dengan menjaga kebersihan diri dengan menerapkan Perilaku Hidup Bersih dan Sehat (PHBS) dan dengan mencuci tangan dengan baik  menggunakan sabun maupun cairan antiseptik.  Praktik PHBS terutama di tempat kerja yang melibatkan banyak orang berkumpul sangat perlu untuk diterapkan. Tujuan dari pengabdian ini adalah untuk membentuk pemahaman masyarakat terutama para karyawan di tempat kerja yaitu di PT. Narmada Awet Muda sebagai salah satu industri Air Minum Dalam Kemasan (AMDK) terhadap penyebaran virus dan pencegahannya terutama virus Corona. Kegiatan pengabdian dilakukan dengan metoda ceramah, diskusi serta demonstrasi dan diikuti oleh sekitar 200 karyawan pabrik PT. Narmada Awet Muda. Hasil kegiatan pengabdian menunjukkan bahwa tingkat pengetahuan dan pemahaman karyawan meningkat (80.8%) terhadap PHBS dan cara cuci tangan yang baik sebagai upaya pencegahan penularan virus Corona.
EVALUASI SIFAT FISIK DAN SKRINING FITOKIMIA TEH BIJI PEPAYA (Carica papaya L.) Maulidia Umami; Pratama, Iman Surya; Sriasih, Made
Jurnal Kedokteran Vol 10 No 2 (2021): Jurnal Kedokteran Juni 2021
Publisher : Faculty of Medicine Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jku.v10i2.522


Latar Belakang: Biji pepaya telah digunakan sebagai antelmintik pada ternak. Teh biji pepaya memiliki aktivitas antiparamphistomum secara in vitro melalui metode adult mortality assay. Karakterisasi teh biji pepaya perlu dilakukan untuk menjamin mutu produk teh yang dihasilkan. Penelitian ini bertujuan untuk menentukan karakteristik fisik dan metabolit sekunder teh biji pepaya (Carica papaya L.). Metode: Kandungan senyawa teh biji pepaya dikarakteristisasi melalui skrining fitokimia. Evaluasi fisik teh biji pepaya meliputi organoleptik, pengukuran pH, penentuan bobot jenis, variasi bobot, dan kadar air. Hasil Penelitian: Teh biji pepaya memiliki rasa pahit, aroma khas, dan berwarna kuning bening, dengan pH 5.61, kadar air 6,876%, bobot jenis 1,000431887 g/ml, dan pada variasi bobo tidak ada bobot yang melebihi 12% limit variasi. Teh biji pepaya mengandung flavonoid dan steroid/triterpenoid. Kesimpulan: Teh biji pepaya memenuhi standar mutu teh. Teh biji pepaya mengandung flavonoid dan steroid/triterpenoid.