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All Journal Jurnal Ilmu Lingkungan Jurnal Kedokteran
Made Sriasih, Made
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Environmental Science Health Professions Medicine & Pharmacology

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Improvements of PCR Amplification of Guanine plus Cytosine-Rich Constructs of Mycobacterium tuberculosis Gene using DMSO Sabrina, Yunita; Lestarini, Ima Arum; Ekawanti, Ardiana; Sriasih, Made; Depamede, Sulaiman N.; Ali, Muhamad
Jurnal Kedokteran Vol 1 No 1 (2012)
Publisher : Faculty of Medicine Universitas Mataram

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Abstract

Vaccine research entered a new era when several useful molecular research tools were established. Instead of attenuated virulent microorganisms or killed virulent microorganisms, effective subunit vaccines were developed using recombinant DNA technology. By using the technology, selected genes of the virulent microorganisms can be amplified, cloned, expressed, and evaluated as vaccine components in challenge studies. However, a major bottleneck with the amplification of functional genes from Mycobacterium tubeculosis containing guanine plus cytosin-rich templates is often hampered by the formation of secondary structures like hairpins and higher melting temperatures. To solve this problem in this research, the amplification reaction was modified by addition of dimethyl sulfoxide (DMSO) into amplification reaction mixtures. It was found that 10% (v/v) of DMSO in the reaction mixture improved the amplification of GC-rich template of M. tuberculosis gene. This result indicating that amplification of unbalanced content of G and C deoxyribonucleotides genome could be improved using low-cost organic molecule, DMSO. Therefore, the DMSO should be widely useful as an enhancer to improve the amplification of GC rich construct from other genome. Keywords: Mycobacterium tubeculosis,vaccine, dimethyl sulfoxide, Guanine-Cytosine
Studi Pendahuluan Cemaran Air Limbah Rumah Potong Hewan di Kota Mataram Aini, Aini; Sriasih, Made; Kisworo, Djoko
Jurnal Ilmu Lingkungan Vol 15, No 1 (2017): April 2017
Publisher : School of Postgraduate Studies, Diponegoro Univer

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (772.078 KB) | DOI: 10.14710/jil.15.1.42-48

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ABSTRAKTujuan penelitian ini adalah untuk mengetahui cemaran baku mutu air limbah Rumah Potong Hewan (RPH) Sapi dan Ayam di Kota Mataram. Semua hasil melebihi baku mutu kecuali nilai pH. Kandungan ALT rerata air limbah RPH sapi 284004 x 104 koloni/ml dan mengandung mikroba jenis E.coli dan Salmonella. Sedangkan air limbah RPH ayam ALT rerata 101596 x 105 koloni/ml dan mengandung kuman jenis E. coli, Pseudomonas aeruginosa dan Klebsiella pneumoniae. Tingginya kandungan BOD, COD, TSS, ammonia, pH, minyak dan lemak maupun cemaran mikroba diduga disebabkan tidak difungsikannya Instalasi Pengolahan Limbah (IPAL) pada RPH sapi dan tidak optimalnya fungsi IPAL di RPH ayam.Kata kunci: RPH sapi, RPH ayam, Baku mutu limbah RPH, Cemaran mikroba, kota Mataram yABSTRACTThe purpose of this study was to determine the contamination of waste water quality standard and microbiologic contamination ofcattle and chicken slaughterhousein Mataram city. TPC average content of waste water of cattle slaughterhouse was 284004 x 104 colonies/ ml, and contained microbial species of E. coli and Salmonella. However, the TPC avarange of wastewater of chicken slaughterhouse accounted for 101596 x 105 colonies/ml and contained microbial species of E. coli, Pseudomonas aeruginosa and Klebsiella pneumoniae. High content of BOD, COD, TSS, ammonia, pH, oil and fats as well as microbial contamination may relate to disfunctionality of Waste Water Treatment Installation (IPAL) in cattle slaughterhouse and too less optimum function of the IPAL in chicken slaughterhouse.Keywords: cattle slaughter house, chicken slaughter house, slaughterhouse waste quality standard, microbial contamination, Mataram cityCitation: Aini, A., Sriasih, M, dan Kisworo, D. (2017). Studi Pendahuluan Cemaran Air Limbah Rumah Potong Hewan di Kota Mataram. Jurnal Ilmu Lingkungan, 15(1), 42-48, doi:10.14710/jil.15.1.42-48
EVALUASI SIFAT FISIK DAN SKRINING FITOKIMIA TEH BIJI PEPAYA (Carica papaya L.) Maulidia Umami; Pratama, Iman Surya; Sriasih, Made
Jurnal Kedokteran Vol 10 No 2 (2021): Jurnal Kedokteran Juni 2021
Publisher : Faculty of Medicine Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jku.v10i2.522

Abstract

Latar Belakang: Biji pepaya telah digunakan sebagai antelmintik pada ternak. Teh biji pepaya memiliki aktivitas antiparamphistomum secara in vitro melalui metode adult mortality assay. Karakterisasi teh biji pepaya perlu dilakukan untuk menjamin mutu produk teh yang dihasilkan. Penelitian ini bertujuan untuk menentukan karakteristik fisik dan metabolit sekunder teh biji pepaya (Carica papaya L.). Metode: Kandungan senyawa teh biji pepaya dikarakteristisasi melalui skrining fitokimia. Evaluasi fisik teh biji pepaya meliputi organoleptik, pengukuran pH, penentuan bobot jenis, variasi bobot, dan kadar air. Hasil Penelitian: Teh biji pepaya memiliki rasa pahit, aroma khas, dan berwarna kuning bening, dengan pH 5.61, kadar air 6,876%, bobot jenis 1,000431887 g/ml, dan pada variasi bobo tidak ada bobot yang melebihi 12% limit variasi. Teh biji pepaya mengandung flavonoid dan steroid/triterpenoid. Kesimpulan: Teh biji pepaya memenuhi standar mutu teh. Teh biji pepaya mengandung flavonoid dan steroid/triterpenoid.
Co-Authors Aini, Aini Ardiana Ekawanti Djoko Kisworo Ima Arum Lestarini Maulidia Umami Muhamad Ali Pratama, Iman Surya Sulaiman N. Depamede, Sulaiman N. Yunita Sabrina, Yunita