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BIOLARVASIDA EKSTRAK ETANOL KULIT NANAS (Ananas comosus L. Merr) TERHADAP LARVA NYAMUK Culex Sp. Juariah, Siti; Irawan, Mega Pratiwi
Unnes Journal of Public Health Vol 6 No 4 (2017): Unnes Journal of Public Health
Publisher : Universitas Negeri Semarang in cooperation with Association of Indonesian Public Health Experts (Ikatan Ahli Kesehatan Masyarakat Indonesia (IAKMI))

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (207.049 KB) | DOI: 10.15294/ujph.v6i4.15842

Abstract

ABSTRAK   Nanas merupakan salah satu komoditi asal Provinsi Riau. Setiap tahun nanas mengalami peningkatan dengan semakin meningkatnya produksi nanas maka limbah yang dihasilkan akan semakin meningkat. Pada limbah kulit nanas diduga terdapat senyawa alkaloid yang dapat membunuh larva nyamuk Culex Sp. Nyamuk yang termasuk dalam genus Culex dikenal sebagai vektor penular arbovirus, demam kaki gajah, dan malaria pada unggas. Pengendalian serangga umumnya dilakukan menggunakan pestisida sintetik. Penggunaan senyawa kimia yang bersifat sintetik sangat berbahaya bagi pengguna yang terpajan. Tujuan dari penelitian ini adalah untuk menentukan efektifitas ekstrak etanol kulit nanas dan mengetahui jumlah larva yang mati dengan menentukan konsentrasi terbaik dari ekstrak etanol kulit nanas (AnanascomosusL.) terhadap larva nyamuk culex Sp. Penelitian ini bersifat Eksperimen Laboratory, yaitu meneliti tentang uji mortalitas larva nyamuk Culex Sp setelah pemberian ekstrak etanol kulit nanas (AnanascomosusL.). Hasil penelitian yang didapatkan bahwa ekstrak etanol kulit nanas dapat dibuktikan pada tingkat konsentrasi 1% angka kematian larva nyamuk telah mencapai 72,5%. Sedangkan pada konsentrasi 4% telah mampu membunuh larva sebesar 97,5%. Kata Kunci        : Ekstraketanol, Kulit nanas, Culex Sp.   ABSTRACT                Pineapple is one of the commodities origin of Riau Province. Each year the pineapple increases with the increasing production of pineapple so the waste generated will increase. In pineapple leaf waste is suspected of alkaloid compounds that can kill the mosquito larvae Culex Sp. The mosquitoes belonging to the genus Culex are known as arbovirus-transmitting vectors, elephant leg fever, and malaria in poultry. Insect control is generally done using synthetic pesticides. The use of synthetic chemicals is very dangerous for exposed users. The purpose of this study was to determine the effectiveness of pineapple ethanol extract and to determine the number of dead larvae by determining the best concentration of pineapple ethanol extract (Ananascomosus L.) on culex mosquito larvae Sp. This research is experimental laboratory, which is examining about mortality test of Culex sp. Mosquito larvae after administration of pineapple ethanol extract (Ananascomosus L.). The results showed that pineapple ethanol extract can be proven at concentration level of 1% mosquito larvae mortality rate has reached 72,5%. While at 4% concentration has been able to kill larvae of 97.5%.   Keywords:Ethanol Extract, Pineapple Skin, Culex Sp.
Isolation and Characterization of Phytase from Chicken Manure Bacteria Irawan, Mega Pratiwi; Nurachman, Zeily
Jurnal Natur Indonesia Vol 15, No 2 (2013)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (339.527 KB) | DOI: 10.31258/jnat.15.2.99-105

Abstract

Cereals in animal feed contain anti-nutrients of phytic acid that has capability of chelating proteins and cations. Phytasecan be employed to reduce phytic acid through hydrolyzing phytic acid into free phosphate group and lower derivate ofinositol phosphate. The aim of the study was to isolate and characterize phytase obtained from chicken manure bacteria.The study included the screening of phytase-producing bacteria from chicken manure, the homology analysis of bacterium,and the determination of phytase activity. Phytase activity was measured from concentration of free phosphate. The resultsshowed that one phytase-producing isolate obtained from chicken manure grew in the medium containing 5% rice branextract at 37°C for 5 d. Based on a phylogeny is tree analysis of the genes related to 16S rRNA, the isolate was identified asAcinetobacter sp. TZ1. The extracellular phytase expressed by Acinetobacter sp. TZ1 exhibited optimum reactions at pH5 and 50°C. The enzyme showed activity of 64,6 nmol mL–1min–1 and specific activity of 236 nmol min–1mg–1. Relativemolecular mass of phytase TZ1 was ~35 kDa. Phytase obtained is potential to improve animal feed quality by hydrolyzingphytic acid.
Identifikasi Jamur Pathogen pada Air Bak Toilet SPBU di Kecamatan Rumbai Kota Pekanbaru Mega Pratiwi Irawan; Siti Juariah; Syarifah Rukmaini
Health Information : Jurnal Penelitian Vol 11 No 2 (2019): Juli-Desember
Publisher : Poltekkes Kemenkes Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (421.2 KB) | DOI: 10.36990/hijp.v11i2.128

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Water is a source of disease transmission. Many water-borne diseases are caused by contamination by bacteria, viruses, parasites and fungi. Fungal contamination of water comes from water sources that are not properly maintained. Lacto Phenol Cotton Blue (LPCB) solution serves to color the mushrooms blue. The purpose of this study was to determine the pathogenic fungi found in the water of gas stations in Rumbai District, Pekanbaru City. This research was conducted using experimental methods in the laboratory. Laboratory tests include planting on PDA (Potato Dextrose Agar) media using the pour plate method. The identification was carried out macroscopically by looking at the shape and color of the colony, and microscopically using the LPCB solution. The results obtained from this study were mostly found Candida fungi and there are also other fungi such as Aspergillus, Trichophyton, Penicillium. From the results obtained, it was concluded that in the toilet water of gas stations in Rumbai District, Pekanbaru City, found pathogenic fungi.
PENGENALAN, PEMANTAUAN DAN PENYULUHAN PENTINGNYA PERSONAL HIGIENE DI SMAN X PEKANBARU Siti Juariah; Eli Yusrita; Darmadi Darmadi; Mega Pratiwi Irawan; Ilham Kurniati
Jurnal Pengabdian Masyarakat Multidisiplin Vol 1 No 3 (2018): Juni
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (42.604 KB) | DOI: 10.36341/jpm.v1i3.424

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Hygiene diartikan sebagai ilmu yg berkenaan dengan masalah kesehatan dan berbagai usaha untuk mempertahankan atau memperbaiki kesehatan.kebersihan lingkungan merupakan salah satu faktor yang sangat mempengaruhi tingkat kesehatan bagi lingkungan dan individu. Pemahaman tentang hygiene masih dikategorikan cukup, hal ini dapat terlihat dari kebiasaan anak-anak yang selalu membuang sampai sembarangan. Tujuan kegiatan ini ialah untuk memberikan pemahaman terhadap pentingnya menjaga kesehatan dan mendeteksi tingkat kebersihan terhadap siswa-sekolah menengah atas. Metode yang digunakan ialah berupa pemantauan kebersihan lingkungan dan penyuluhan. Hasil yang diperoleh menunjukkan bahwa lingkungan sekolah menengah atas sudah masuk dalam kategori cukup bersih namun ada beberapa hal yang harus dibenahi yakni pengetahuan terhadap pentingnya menjaga kesehatan serta dampak yang akan diperoleh jika tidak bisa mengendalikan kebersihan secara berkesinambungan.
Kadar Asam Urat Penderita Tuberkulosis Sebelum dan Sesudah Mengkonsumsi Obat Anti Tuberkulosis di Rumah Sakit X Mega Pratiwi Irawan
Jurnal Sains dan Teknologi Laboratorium Medik Vol 6 No 1 (2021): April (2021)
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v6i1.75

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Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis. Treatment of tuberculosis contained at least four types of drugs such as Rifampicin, Ethambutol, Streptomycin and Pyrazinamide. Ethambutol and Pyrazinamide can cause an increase in uric acid levels. This study aims to describe the uric acid levels of tuberculosis patients of Rumah Sakit X. This type of research was Cross Sectional by taking secondary data on the patient's medical record at Rumah Sakit X. The results of laboratory tests from 20 tuberculosis patients who consumed anti tuberculosis drugs showed that uric acid levels above normal after taking the drug for 2 months was suffered by 5 male patients (25%) and 7 female patients (35%). Uric acid levels above normal was suffered by 4 male patients (20%) and 7 female patients (35%) after taking the drug for 4 months. Uric acid levels above the normal was suffered by 4 male patients (20%) and 6 female patients (30%) after taking the drug for 6 months. By using ANOVA test, get t count 0,000 <0,005. The conclusion of this study is that there is a difference between uric acid levels before treatment and after treatment.
EFEKTIFITAS EKSTRAK ETANOL KULIT NANAS (Ananas Comosus L. Merr) terhadap Trichophyton mentaghrophytes Siti Juariah; Mega Pratiwi Irawan; Yuliana Yuliana
JURNAL FARMASI DAN MAKANAN Vol 1 No 2 (2018): Journal Of Pharmacy and Science
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/jops.v1i2.486

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Ringworm of the skin caused by a Trichophyton mentaghrophytes, Drugs that are commonly used are synthetic drugs, synthetic drugs can have a negative impact. By because it, research on natural antifungal substances continue to do that with the use of pineapple skin.A pineapple skin waste processed pineapple industry, namely the remainder of the meat and fruit contains secondary metabolites are flavonoids, phenolics, saponins, triterpenoids, steroids and alkaloids. The purpose of this study to determine the content of secondary metabolites contained in the pineapple skin and for the inhibition of Trichophyton mentaghrophytes using experimental laboratoryin vitro. Pineapple bark extract prepared in various concentrations of 10%, 15%, 20% and 25%, then test the inhibition ofTrichophyton mentaghrophytesand incubated temperature 25o C for 5day. Based on the results obtained percentage pineapple skin extractfungal inhibition test Trichophyton mentaghrophytesthe concentration 10%, 15%, 20% and 25%, respectively, are22%, 37%, 46% and 56% compared to the positive control.Based on this research, pineapple bark extract containing flavonoids, phenolics, saponins, triterpenoids, steroids and alkaloids. The results of this study can be concluded that the extract of pineapple skin Trichophyton mentaghrophyteswhich is characterized by the formation of inhibit zone.
TOKSISITAS EKSTRAK ETANOL KULIT JENGKOL (Pithecelleobium jiringa) TERHADAP LARVA UDANG (Artemia salinia Leach) DENGAN METODE Brine Shrimp Lethality Test (BSLT) alfin surya; Mega Pratiwi Irawan; Yolanda Yolanda; Zaiyar zaiyar
Klinikal Sains : Jurnal Analis Kesehatan Vol 10 No 1 (2022): Juni
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v10i1.2499

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Tanaman jengkol merupakan salah satu jenis tumbuhan yang tersebar dimasyarakat memiliki manfaat sebagai senyawa aktif yang mampu dimanfaatkan sebagai obat tradisional. Kulit memiliki beragam manfaat yaitu dapat menghentikan pendarahan, obat luka bakar, dan sebagai antiseptik. Pada kulit jengkol mengandung senyawa alkaloid, flavonoid, saponin, tanin dan glikosida. Senyawa yang terdapat pada tanaman kulit jengkol tersebut dapat berperan sebagai antikanker. Tujuan penelitian ini adalah untuk mengetahui toksisitas ekstrak etanol kulit jengkol (Pithecelleobium jiringa) terhadap larva udang (Artemia salina Leach), Metode yang digunakan adalah Brine Shrimp Lethality Test (BSLT). Jenis penelitian yang digunakan adalah kuantitatif. Hasil penelitian ini menunjukkan bahwa ekstrak etanol kulit jengkol (Pithecelleobium jiringa) bersifat toksik sehingga berpotensi untuk antikanker, dibuktikan dengan menghitung log konsentrasi didapatkan nilai LC50 yaitu 251 ppm. Hal ini menunjukkan bahwa ekstrak toksik terhadap larva udang (Artemia salina Leach).
GAMBARAN KADAR KOLESTEROL PADA PEMINUM KOPI DI KELURAHAN TELUK AIR KECAMATAN KARIMUN Eli Yusrita; Mega Pratiwi Irawan; Dwinur Aini
Klinikal Sains : Jurnal Analis Kesehatan Vol 10 No 1 (2022): Juni
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v10i1.2507

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Kopi merupakan minuman dengan kandungan polifenol yang sangat tinggi. Polifenol di dalam kopi sangat kaya dengan caffeoylquinic acids, feruloyquinic acids, dan dicaffeoylquinic acids. Senyawa polifenol yang paling banyak terdapat dalam kopi adalah cholorogenic acid. Berdasarkan hasil penelitian yang telah dilakukan responden yang konsumsi kopi dalam jangka Panjang dapat meningkatkan kadar kolesterol dalam tubuh. Tujuan penelitian ini adalah untuk mengetahui kadar kolesterol pada peminum kopi di kelurahan teluk air kecamatan karimun. Jenis penelitian ini adalah menggunakan metode deskriptif yang memberikan suatu gambaran secara objektif. Hasil penelitian menunjukkan bahwa dari 30 responden, responden yang berjenis kelamin perempuan hasil tinggi berjumlah 8 responden dan normal berjumlah 7 responden, responden yang berjenis kelamin laki laki dengan hasil tinggi berjumlah 7 responden dan hasil normal berjumlah 8 responden.
Edukasi Kanker Serviks dan Efektivitas Vaksin HPV Sejak Dini di SMAN 2 Pekanbaru Muhammad Faisal; Alief Dhuha; Mega Pratiwi Irawan; Sellia Juwita; Putri Wulandini
Jurnal Abdimas Kesehatan (JAK) Vol 4, No 2 (2022): Juni
Publisher : Sekolah Tinggi Ilmu Kesehatan Baiturrahim Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1086.242 KB) | DOI: 10.36565/jak.v4i2.359

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In 2013, the number of cervical cancer cases in Riau Province reached 894 cases. In 2020, RSUD Arifin Achmad Riau Province reported the cervical cancer was the third rank below breast and ovarian cancer. Cervical cancer has major risk factor named by HPV virus. HPV vaccine has been reported to adequate to eliminate HPV viruses. The Indonesian government has provided the health facilities to decrease cervical cancer sufferers by providing HPV vaccines. However, vulnerable women are not ready to be vaccinated.  Education level is one of the factors. Hence, we try to intervene the mind of SMAN 2 Pekanbaru female students by offer early knowledge regarding the risk factors of cervical cancer, how to prevent as well as socialize HPV vaccine effectivity and safety. Educational and outreach activities were carried out by material presentation method by a direct meeting which interactive and educative. Feedback data collection from the activities was given through the Google Form platform. This activity was held on Monday, 22nd November 2021 at 09.00 – 10.35 West Indonesian Time, joined by 20 female students of SMAN 2 Pekanbaru which included three grade levels, X, XI, and XII class. According to the results of the questionnaire, we obtained the improvement of cervical cancer and HPV vaccine information. Moreover, this activity upgraded the awareness of cervical cancer prevention, by as much as 25% (N=12). We may conclude that education on cervical cancer is tremendously important to young women as our effort to decrease cervical cancer cases in the future.
Gambaran Leukosit pada Sedimen Urine Ibu Hamil Hotmauli Hotmauli; Imelda Fitri; Mega Pratiwi Irawan; Sheryn Fildzah Azhari
Jurnal Penelitian Perawat Profesional Vol 3 No 3 (2021): Agustus 2021, Jurnal Penelitian Perawat Profesional
Publisher : Global Health Science Group

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/jppp.v3i3.544

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Kejadian infeksi saluran kemih tertinggi pada kehamilan trimester III yaitu 36,9 %. Leukosit urine merupakan salah satu pemeriksaan penunjang pada penyakit ginjal dan infeksi saluran kemih. Tujuan penelitian ini untuk mengetahui gambaran leukosit pada sedimen urine Ibu hamil di Kelurahan Babussalam Kota Duri. Penelitian ini menggunakan desain penelitian deskriptif dengan sampel penelitian ibu hamil yang memenuhi kriteria inklusi dan eksklusi berjumlah 27 responden. Gambaran leukosit urine dengan menggunakan metode mikroskopis urine dengan meggunakan mikroskop. Analisa data hasil pemeriksaan leukosit pada sedimen urine ibu hamil disajikan dalam bentuk deskriptif berupa gambar dan tabel. Hasil penelitian didapat kansebanyak 8 responden (30%) memiliki jumlah leukosit ˃5/LPB dan 19 responden (70%) memiliki jumlah leukosit 0–5/LPB. Dari 8 responden (30%) yang leukosit urine ˃5/LPB yaitu 1 responden dari trimester I, 1 responden dari trimesrter II dan 6 responden dari Trimester III. Ibu hamil trimester III mengalami leukosit urine tidak normal terbanyak yang disebabkan oleh perubahan yang terjadi selama kehamilan, jumlah leukosit yang tidak normal dapat menyebabkan infeksi saluran kemih bahkan beresiko terjadinya persalinan prematur dan gangguan pertumbuhan janin.