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All Journal Jurnal Sains Materi Indonesia Folia Medica Indonesiana Indonesian Journal of Tropical and Infectious Disease MEDISAINS Dental Journal (Majalah Kedokteran Gigi) Indonesian Journal of Chemistry Jurnal Layanan Masyarakat (Journal of Public Service) Indian Journal of Forensic Medicine & Toxicology I-Com : Indonesian Community Journal Jurnal Rekayasa Kimia & Lingkungan Prosiding Seminar Nasional Unimus

Prihartini Widiyanti

Biomedical Engineering Study Program, Department Of Physics, Faculty Of Science And Technology, Universitas Airlangga Institute Of Tropical Disease, Universitas Airlangga

Author-ID : 508655

Agriculture, Biological Sciences & Forestry Humanities Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Engineering Environmental Science Health Professions Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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EFFECT OF FREE ALKALOID AND NON-FREE ALKALOID ETHANOL 70% EXTRACT OF JUSTICIA GENDARUSSA BURM F. LEAVES AGAINST REVERSE TRANSCRIPTASE HIV ENZYME IN VITRO AND CHEMICAL COMPOUND ANALYSIS Prajogo, Bambang; Widiyanti, Prihartini; Riza, Hafrizal
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 7 (2015)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v5i7.1203

HIV-AIDS is a global problem and the deadliest disease in the world. One of HIV and AIDS prevention strategy can be done with traditional medicine research program from natural resource that has anti-HIV AIDS activity. It has been found that 70% ethanol extract of Justicia gendarussa Burm.f leaves, alkaloid free and alkaloid non-free, has a strong inhibitory activity against HIV reverse transcriptase enzyme, as an effort to find a solution in the face of HIV AIDS prevalence that is still high with problem of HIV-AIDS treatment such as side effects and resistances. Justicia gendarussa had already known for having an effect anti-HIV and therefore we were looking at the mechanism of inhibition of HIV Reverse Transcriptase enzyme. Both types of extracts were tested in vitro using ELISA technique and analysed chemical content of Gendarusin A as anti-HIV using high performance liquid chromatography. ELISA test results obtained percent inhibition, respectively for 254.2, 254.2, 235.6, and 279.7 for the concentration of 5 ppm, 10 ppm, 15 ppm and 20 ppm of free alkaloid extract and 169.0, 164.0, 130.5 and 369.5 for the concentration of 5 ppm, 10 ppm, 15 ppm and 20 ppm of non-free-alkaloid extract. The results of high performance liquid chromatography obtained Gendarusin A in the free-alkaloid extract at retention time 8.402 minutes and non-free alkaloid extract at retention time 8.381. Therefore, these results concluded that the Justicia gendarussa Burm.f can be a useful resource for the isolation and development of new anti-HIV.

CYTOTOXICITY OF JUSTICIA GENDARUSSA BURM F. LEAF EXTRACTS ON MOLT-4 CELL Widiyanti, Prihartini; Prajogo, Bambang; Hikmawati, Ni Putu Ermi
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 7 (2015)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v5i7.1207

Justicia gendarussa Burm f. (Acanthaceae) is known for its activity as a male contraceptive and anti-HIV properties. The present study was designed to evaluate extracts of J. gendarussa for cytotoxicity activity against MOLT-4 cells. The cytotoxic activity of the fractionated-extract and 70% ethanol extracts of J. gendarussa leaves on MOLT-4 cells were evaluated using a WST-1 assay. The treatment cells, control cells without treatment and control media were also tested in duplicate. The absorbance was measured at a wavelength of 450 nm using a microplate absorbance reader (Bio-Rad). The average absorbance measures formazan produced by viable cells that metabolize the WST-1 reagent. Then the data was analyzed with regression analysis Microsoft Excel 2007 program to determine the concentration with 50% cell viability (50% Cytotoxicity Concentration, CC50). The CC50 values of the fractionated-extract and 70% ethanol extract of J. gendarussa leaves were 94 Î¼g/ml and 78 Î¼g/ml, respectively. The cytotoxicity of fractionated-extract and 70% ethanol extract of J. gendarussa leaves were not significantly different (p > 0.05). It can be concluded that the fractionated-extract and 70% ethanol extract of J. gendarussa leaves are not toxic to MOLT-4 cells.

Evaluation of surface modified PLLA-collagen hollow fiber as vascular graft Mayasari, Dita Ayu; Widiyanti, Prihartini; Rudyardjo, Djony Izak
MEDISAINS Vol 17, No 2 (2019)
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/medisains.v17i2.5259

Background: Cardiovascular disease as the first silent killer in the world is related to atherosclerosis plaque. Coronary artery bypass grafting as a gold standard for severe atherosclerosis needs alternative materials to replace the narrowed artery due to plaque. PLLA is one of the biodegradable polymers which is suitable for the vascular graft, but some of its weaknesses should be addressed.Objective: The aim of this research is to improve PLLA characteristics for a vascular graft candidate by using collagen and chitosan.Methods: This study was an experimental study that consisted of two steps, preparation of hollow fiber and evaluation of its characteristic. This research evaluated the effect of chitosan concentration against cytotoxicity and hemocompatibility using MTT assay and hemolytic test.Results: The result showed that chitosan could improve the biological characteristics of hollow fiber PLLA-collagen, non-toxic, and non-hemolytic.Conclusions: There is a good effect on the biological characteristics of PLLA-Collagen hollow fiber by adding chitosan on the surface of PLLA-Collagen hollow fiber.

VARIASI KOMPOSISI NANO HIDROKSIAPATIT PADA POLY(1,8-OCTANEDIOL-COCITRATE) (POC) SEBAGAI BIODEGRADABLE BONE SCREW Fitriyatul Qulub; Prihartini Widiyanti; Jan Ady
Jurnal Sains Materi Indonesia Vol 16, No 4: JULI 2015
Publisher : Center for Science & Technology of Advanced Materials - National Nuclear Energy Agency

VARIASI KOMPOSISI NANO HIDROKSIAPATIT PADA POLY(1,8-OCTANEDIOL-COCITRATE) (POC) SEBAGAI BIODEGRADABLE BONE SCREW . Tingginya kasus fraktur tulang sekitar 300-400 ditangani dengan metode fiksasi interna menggunakan bone screw. Diperlukan inovasi biomaterial yang memiliki sifat utama biodegradabel agar tidak memerlukan reoperation dan biokompatibel terhadap jaringan tubuh. Telah dilakukan penelitian mengenai variasi komposisi nano hidroksiapatit (HA) berasal dari ekstrak sisik ikan kakap pada Poly (1,8-octanediol-Co-Citrate) (POC) sebagai Biodegradable Bone Screw. Penelitian ini bertujuan mensintesis POC dan mengkarakterisasi pengaruh komposisi HA terhadap sifat mekanik dan biokompatibilitas komposit POC-HA. Sintesis POC dilakukan dengan metode polimerisasi kondensasi, terbentuk ikatan ester gugus C=O stretch pada 1731 cm-1 melalui uji gugus fungsi dari POC pra polimer. Pra polimer POC dikompositkan dengan nano-HA pada variasi komposisi 62%,65%,68%dan 71%, dilanjutkan perlakuan postpolymerization. Komposit POC-HA dilakukan karakterisasi uji kekerasan dan uji degradasi. Hasil pengujian menunjukkan komposisi nanopartikel HA memberikan pengaruh sifat mekanik dan biokompatibilitas. Seluruh sampel memiliki kuat tekan berkisar 3,64-9,56 MPa sesuai dengan kuat tekan tulang cancellous manusia (2-12 MPa). Melalui analisis Energy Dispersive X-Ray Spectroscopy (EDX), uji degradasi sampel terbukti kebioaktifitasan mineral apatit sebesar 7,5% (At%) dan terputusnya rantai C-X dari polimer POC sebesar 5,95% (At%) selama 4 minggu inkubasi dalam Simulated Body Fluid (SBF). Berdasarkan hasil karakteristik penelitian ini, komposit POC-HAberpotensi sebagai kandidat biodegradable bone screw.

Physical characteristic of brown algae (Phaeophyta) from madura strait as irreversible hydrocolloid impression material Prihartini Widiyanti; Siswanto Siswanto
Dental Journal (Majalah Kedokteran Gigi) Vol. 45 No. 3 (2012): September 2012
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Brown algae is a raw material for producing natrium alginates. One type of brown algae is Sargassum sp, a member of Phaeophyta division. Sargassum sp could be found in Madura strait Indonesia. Natrium alginate can be extracted from Sargassum sp. The demand of alginate in Indonesia is mainly fulfilled from abroad, meanwhile Sargassum sp is abundantly available. Purpose: The purpose of study were to explore the potency of brown alga Sargassum sp from Madura strait as hydrocolloid impression material and to examine its physical characteristic. Methods: The methods of research including extraction natrium alginate from Sargassum sp, synthesis of dental impression material and the test of porosity, density, viscosity, and water content of impression material which fulfilled the standard of material used in clinical application in dentistry. Results: Extraction result of Sargassum sp was natrium alginate powder with cream colour, odorless, and water soluble. The water content of natrium alginate was 21.64% and the viscosity was 0.7 cPs. The best porosity result in the sample with the addition of trinatrium phosphate 4% was 3.61%. Density value of impression material was 3 gr/cm3. Conclusion: The research suggested that brown algae Sargassum sp from Madura strait is potential as hydrocolloid impression material, due to its physical properties which close to dental impression material, but still need further research to optimize the physical characteristic.Latar belakang: Alga coklat adalah sumber bahan baku material natrium alginat. Salah satu jenis alga coklat adalah Sargassum sp yang merupakan anggota divisi Phaeophyta. Sargassum sp dapat ditemukan di Selat Madura Indonesia. Natrium alginat dapat diekstraksi dari Sargassum sp. Kebutuhan akan bahan ini di Indonesia sebagian besar dipenuhi dari impor, padahal ketersediaan Sargassum sp di Indonesia sangat melimpah. Tujuan: Penelitian ini bertujuan untuk mengeksplorasi potensi alga coklat Sargassum sp dari Selat Madura sebagai bahan cetak hidrokoloid dan meneliti karakteristik fisiknya. Metode: Tahap pertama adalah ekstraksi natrium alginat dari Sargassum sp, tahap kedua yaitu sintesis bahan cetak gigi dan menguji karakteristik bahan seperti porositas, densitas, viskositas, kadar air, bahan cetak yang memenuhi standar bahan yang digunakan dalam aplikasi klinis bidang Kedokteran Gigi. Hasil: Hasil ekstraksi berupa natrium alginat bubuk dengan warna krem, tidak berbau, dan dapat larut dalam air. Kadar air natrium alginat sebesar 21,64% dengan viskositas 0,7 cPs. Porositas terbaik dalam sampel dengan penambahan trinatrium fosfat 4% yaitu 3,61%. Nilai densitas bahan cetak 3 gr/cm3. Kesimpulan: Penelitian ini menunjukkan bahwa alga coklat Sargassum sp dari Selat Madura memiliki potensi sebagai bahan cetak hydrocolloid kedokteran gigi karena memiliki karakter fisik yang mirip dengan bahan cetak kedokteran gigi, namun masih memerlukan penelitian lebih lanjut untuk mengoptimalkan karakter fisiknya.

EFFECT OF DEACETYLATION DEGREES VARIATION ON CHITOSAN NERVE CONDUIT FOR PERIPHERAL NERVE REGENERATION Fitriyatul Qulub; Prihartini Widiyanti; Hendita Nur Maulida; Ludita Woro Indrio; Tia Rahayu Wijayanti
Folia Medica Indonesiana Vol. 53 No. 2 (2017): JUNE 2017
Publisher : Faculty of Medicine, Universitas Airlangga

Broken nerves could regenerate when exposed to simple injuries by using a nerve conduit that has appropriate physiological and mechanical ability to support the nerves regeneration around the fissure of trauma. One of the biopolymer for the conduit composition is chitosan because it is biocompatible, biodegradable, non-toxic, and has similarity structure as natural glycosaminoglycans. The aim of research is to synthesize chitosan with variation of Degrees of Deacetylation (DD) and characterize the DD influence on mechanical properties and biocompatibility. Research design is prospective observational. Chitosan was treated with a decrease in the DD method and an increase in the temperature with the strength of alkaline solution, which was NaOH solution with concentrations of 5%, 20%, 35%, and 50% within 2 hours with a heating temperature of 95°C. The results of each DD variation were 23.24, 46.55, 53.48, and 55.06. It was characterized by tensile test with tensile strength values of 0.25 - 1.18 MPa. The degradation test results tend to decrease with the increasing concentration of NaOH proving that samples are biodegradable. The surface morphology of samples shows a pore range of 61.52 μm - 220.3 μm. The best result is the chitosan sample with 35% NaOH because due to the tensile characteristic and a pore in accordance with normal standard. Tensile strength is around 0.41 MPa - 3.69 MPa and pore size around 40 μm – 250 μm to accelerate nerve regeneration. The results are expected to provide alternative solution of nerve conduit development for peripheral nerve defects.

In Vitro Characterization of Poly-Glycolyc Lactic-Co Acid (PLGA) –Collagen Based on Red Snapper Fish Scales (Lutjanus Sp.) Coating Chitosan as Duramater Artificial Candidate Hajria Jabbar; Prihartini Widiyanti; Adanti Wido Paramadini; Dina Kartika Putri; Andini Isfandiary
Folia Medica Indonesiana Vol. 56 No. 3 (2020): September
Publisher : Universitas Airlangga

Head trauma was the third cause of deaths that have a high rank that can make serious head injury for 25.5%-54.9%. This study has been conducted by making a replacement layer of the brain (dura) to overcome the impact of dural defect by utilizing waste fish scales red snapper (Lutjanus sp.). Synthesis brain membranes lining processed by casting method with each various concentrations of chitosan coating of 1%, 1.5%, and 2% then dried using vacuum dry. The samples then were characterized by tensile test, FTIR, SEM and MTT Assay. FTIR test results showed that red snipperscales can produce collagen powder at amide A group with stretching of –NH functional group, amide B group has stretching of CH2 assymetry, amide I area, amide II and amide III area which show –NH bonding. Tensile test results showed that the combination between PLGA-Collagen Chitosan Coating 2% produced the highest tensile strength is 4.8 MPa which meet the standards of human duramater strength. MTT Assay results showed that the dural membrane produced no toxic seen from living cells reached 98.32%. Poly - Glycolyc Lactic - Co Acid (PLGA) - collagen coating chitosan based on red snapper fish scales (Lutjanus sp.) composites has potency as duramater artificial candidate due to the chemistry, biological and physical characteristics.

BASIC MECHANISM OF HYPERBARIC OXYGEN IN INFECTIOUS DISEASE Prihartini Widiyanti
Indonesian Journal of Tropical and Infectious Disease Vol. 2 No. 1 (2011)
Publisher : Institute of Topical Disease Universitas Airlangga

Hyperbaric oxygen therapy (HBOT) is the inhalation of 100 percent oxygen inside a hyperbaric chamber that is pressurized to greater than 1 atmosphere (atm). HBOT causes both mechanical and physiologic effects by inducing a state of increased pressure and hyperoxia. HBOT is typically administered at 1 to 3 atm. While the duration of an HBOT session is typically 90 to 120 minutes, the duration, frequency, and cumulative number of sessions have not been standardized. HBO has been use widely in treating gangrene diabetic, stroke, osteomyelitis and accelerating wound healing. The use of HBO in infectious disease is wide, so the mechanism of hyperbaric oxygen in infectious disease should be well-understand. This understanding could bring the proper and wise management of infectious disease and to prevent the side effect of each therapy.

USING LEARNING VECTOR QUANTIZATION METHOD FOR AUTOMATED IDENTIFICATION OF MYCOBACTERIUM TUBERCULOSIS Endah Purwanti; Prihartini Widiyanti
Indonesian Journal of Tropical and Infectious Disease Vol. 3 No. 1 (2012)
Publisher : Institute of Topical Disease Universitas Airlangga

In this paper, we are developing an automated method for the detection of tubercle bacilli in clinical specimens, principally the sputum. This investigation is the first attempt to automatically identify TB bacilli in sputum using image processing and learning vector quantization (LVQ) techniques. The evaluation of the learning vector quantization (LVQ) was carried out on Tuberculosis dataset show that average of accuracy is 91,33%.

DIGITAL DETECTION SYSTEM DESIGN OF MYCOBACTERIUM TUBERCULOSIS THROUGH EXTRACTION OF SPUTUM IMAGE USING NEURAL NETWORK METHOD Franky Arisgraha; Prihartini Widiyanti; Retna Apsari
Indonesian Journal of Tropical and Infectious Disease Vol. 3 No. 1 (2012)
Publisher : Institute of Topical Disease Universitas Airlangga

Tuberculosis (TBC) is an dangerous disease and many people has been infected. One of many important steps to control TBC effectively and efficiently is by increasing case finding using right method and accurate diagnostic. One of them is to detect Mycobacterium Tuberculosis inside sputum. Conventional detection of Mycobacterium Tuberculosis inside sputum can need a lot of time, so digitallydetection method of Mycobacterium Tuberculosis was designed as an effort to get better result of detection. This method was designed by using combination between digital image processing method and Neural Network method. From testing report that was done, Mycobacterium can be detected with successful value reach 77.5% and training error less than 5%.

Co-Authors - Aminatun - Siswanto Achmad Ardianto Achmad Ardianto, Achmad Adanti Wido Paramadini Adiana Mutamsari Witaningrum Adri Supardi Agung Prabowo Ahmad Fadli Akfiny Hasdi Aimon Akhmad Jayadi Akhmad Jayadi Alfian Pramudita Putra Andhi Baskoro Andini Isfandiary Anggraini Barlian Arie Wibowo Bambang Prajogo Bambang Prajogo Eko Wardoyo Bella Wahyu Sukma Wati Bimo Aksono Bimo Aksono Bimo Dwi Lukito Bimo Dwi Lukito, Bimo Dwi Bramantono - Budi Utomo Budi Utomo Cindy Narita Kusuma Astuti DENI NOVIANA Dina Kartika Putri Dio Nurdin Setiawan Dio Nurdin Setiawan, Dio Nurdin Dita Ayu Mayasari, Dita Ayu Djony Izak Rudyardjo Dyar Sasmito Dyar Sasmito, Dyar Eko Budi Kuntjoro Eko Budi Kuntjoro, Eko Budi Endah Purwanti Fathan Aditya Sanjaya Fatkhunisa Rahmawati Fatkhunisa Rahmawati Fatkhunisa Rahmawati, Fatkhunisa Fa’ilal Chusna Fendy Purwanda Fendy Purwanda, Fendy Ferdiansyah Ferdiansyah Ferdiansyah Ferdiansyah Fitriyatul Qulub Fitriyatul Qulub Fitriyatul Qulub Fitriyatul Qulub Fitriyatul Qulub Franky Arisgraha Franky Arisgraha, Franky Gadis Meinar Sari Gadis Meinar Sari Ganis Tjahjono Hafrizal Riza Hafrizal Riza, Hafrizal Hajria Jabbar Helmi Majid Ar Rasyid Hendita Nur Maulida Heni Ismawati Heri Purnobasuki Heri Purnobasuki, Heri Herry Wibowo Jan Ady Jan Ady Julieta Kartikasari Juniastuti Juniastuti Juniastuti Juniastuti Kholifatul Wanda Putri Kholifatul Wanda Putri, Kholifatul Wanda Kirana Anugerah Putri Ludita Woro Indrio M. Qushay M. Qushay, M. Maria Inge Lusida Maria Inge Lusida Mirzaq Hussein Anwar Mirzaq Hussein Anwar, Mirzaq Hussein Muhammad Bagas Ananda Muhammad Vitanata Arfijanto Nabila Safira Khairina Nasronudin Nasronudin Nasronudin Nasronudin Nasronudin Nasronudin Nasronudin Nasronudin Nasronudin Nasronudin Ni Putu Ermi Hikmawati Ni Putu Ermi Hikmawati, Ni Putu Ermi Nilna Faizah Fiddarain Nilna Faizah Fiddarain, Nilna Faizah Radicha Arnanda Putri Indrawati Retna Apsari Retno Indrawati Roestamadji Retno Pudji Rahayu Satrio Adhitioso Satrio Adhitioso Sherina Pradita Eka Wahyudi Siswanto Siswanto Siswanto Siti Irma Mashitah Siti Qamariyah Khairunisa Siti Qamariyah Khairunisa, Siti Qamariyah Siti Qomariyah Khairunisa Siti Qomariyah Khairunisa, Siti Qomariyah Tami Bachrurozy Tarissa Diandra Putri Wibowo Tia Rahayu Wijayanti Tomohiro Kotaki Tomohiro Kotaki Tomohiro Kotaki, Tomohiro Tri Arif Sardjono Tri Arif Sardjono, Tri Arif Tria Refi Indriani Veren Audia Nurmansya Wulandari Prima Devi Yufan Fibriawan Yufan Fibriawan, Yufan

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