The rapid epidemic growth of HIV is continuing in Indonesia. There are some factors which have influenced the spreading of thisÂ epidemic in Indonesia, such as the poor awareness to avoid unsafe free sex attitude and the sharing of needles and syringes amongÂ intravenous drug users (IDUs). The sexual transmission of HIV has also apparently increased in Tulungagung. Commercial sex workersÂ play a significant role in the spread of HIV in Tulungagung. People in Tulungagung have worked at other countries as IndonesianÂ migrants. This condition can cause the increase number of HIV-1 case and the possibility of genetic variation (subtype) HIV-1 inÂ Tulungagung. This research is aimed to analyze the subtype and to determine estimation of transmission mode on infected patient ofÂ HIV-1 and AIDS who came to Seruni clinic Dr. Iskak hospital in Tulungagung. 40 HIV?AIDSpatients were interviewed to determineÂ the subtype and the transmission mode. The results showed that 14 of 40 plasma samples (35%) were successfully to amplified andÂ sequenced. OverallCRF01-AE wereidentified as predominant subtype among HIV/AIDS patients in Tulungagung. Based on individualÂ information, 31 of 40 subjects (77%) were heterosexual transmission.