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Ratna Yuliani
Universitas Muhammadiyah Surakarta
Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Environmental Science Health Professions Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health Social Sciences Other

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AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAN FRAKSI KULIT BATANG BELIMBING WULUH (Averrhoa bilimbi Linn.) TERHADAP BAKTERI Klebsiella pneumoniae DAN Staphylococcus epidermidis BESERTA BIOAUTOGRAFINYA , MSi., Dr. Muhtadi; Ambarwati, Ria; Yuliani, Ratna
Biomedika Vol 4, No 2 (2012): Biomedika Agustus 2012
Publisher : Universitas Muhamadiyah Surakarta

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Abstract

Belimbing wuluh (Averrhoa bilimbi Linn.) is a tropical plant that has antibacterial properties. The purpose of this study was to test the antibacterial activity of bark Belimbing wuluh against Klebsiella pneumoniae and Staphylococcus epidermidis and their bioautography. Extraction methods used to research is method maceration with a solvent ethanol 96 %. Fractinations done by method partition liquid-liquid with a separating funnel. Test performed in this research covering identi� cation bacteria, the sensitivity bacteria, antibacterial activity, thin layer chromatography, bioautography. The result of antibacterial activity ethanol extract of disk diffusion method with concentrations 400 μg/disk, 800 μg/disk, 1600 μg/disk is 8±0,5; 10,34±0,58; 12,17±0,76 on Klebsiella pneumoniae, 10,17±0,29; 11±0; 11.5±0 on Staphylococcus epidermidis, n-hexane fraction with concentration 400 μg/disk, 800 μg/disk, 1600 μg/disk is 8,34±0,29; 9,34±0,29; 10,84±0,76 on Klebsialla pneumoniae, 8,5±0,5; 9,34±0,29; 10,67±0,29 on Staphylococcus epidermidis, ethyl acetate fraction with concentration 400 μg/disk, 800 μg/disk, 1600 μg/disk is 9,17±0,29; 10,34±0,29; 11,17±0,29 on Klebsiella pneumoniae and 9,5±0,5; 10,67±0,29; 12,67±1,26 on Staphylococcus epidermidis, ethanol-water fractions with concentration 400 μg/disk, 800 μg/ disk, 1600 μg/disk is 8,17±0,29; 9,17±0,29; 10±0 on Klebsiella pneumoniae, 9±0; 9,67±0,29; 10,34±0,29 on Staphylococcus epidermidis. The TLC show chemical compounds contained in the ethanol extract, n-heksan fraction, ethyl acetate fraction, and ethanol-water fraction is a compound of the saponins, alkaloids, � avonoids and phenolic. Bioautography showed that ethanol extracts, n-heksan faction, ethyl acetate fraction, and etanol-airfaction Belimbing wuluh (Averrhoa bilimbi Linn.) bark have not antibacterial activity because there is no clear area around on plate TLC.Keywords: Belimbing wuluh (Averrhoa bilimbi Linn.), ethanol extract, fractination, antibacterial, bioautogra� .
EFEK ANTIINFLAMASI INFUSA RIMPANG TEMU PUTIH (Curcuma zedoaria (Berg) Roscoe) PADA TIKUS YANG DIINDUKSI KARAGENIN Sujono, Tanti Azizah; Patimah, Raudatul; Yuliani, Ratna
Biomedika Vol 4, No 2 (2012): Biomedika Agustus 2012
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

In-flammation is the body’s reaction to foreign substances that enter the body with signs of redness, heat, swelling, pain, and impaired organ functions. Curcuma rhizome including medicinal plants that have properties relieve the pain and inflammation of the skin. The purpose of this research was to examine the anti-iflammation effect of Curcuma zedoaria (Berg) Roscoe Rhizome infusion on carrageenan induced paw edema in rats. This research used experimental method with completely randomized design. Twenty five healthy Wistar male rats, 2-3 months old and 150-250 g divided into five groups and each group consist of 5 rats. Rats in group I that served as negative control were given 2.5 mL/200gbw of aquadest. Rats in group II as positive control were given sodium diclofenac with dose of 6.75 mg/kgbw. Group III, IV, and V were given Curcuma zedoaria (Berg) Roscoe Rhizome infusion with dose of 0.625, 1.250 and 2.500 g/kgbw, respectively. Treatments were given per oral 1 hour before injection of 0.1 ml carrageenan 1% subplantar. Rat paw edema volume was measured before and after carrageenan injection. The measurement was done every 0.5 hour for 6 hours observation. Area Under the Curve (AUC) that is calculated from paw edema volume data, was use to calculate percentage of anti-inflammatory effect. Data were analyzed with one way Anova and Least Signifi cant Difference with confi dence level 95%. Infusion of Curcuma zedoaria Rhizome with doses of 0,625, 1,250, and 2,500 g/kgbw had anti-inflammatory effect in Wistar male rats which were induced by Carrageenan 1%. Percentage of anti-inflammation effect (44,16+5,11)%, (48,70+7,05)%, (59,09+9,61)% respectively and the effects were equivalent with positive control.Key words: Anti-inflammation, Curcuma zedoaria (Berg) Roscoe, infusion, Carrageenan.
AKTIVITAS ANTIBAKTERI MINYAK ATSIRI DAUN JERUK PURUT (Citrus hystrix) TERHADAP Staphylococcus aureus DAN Escherichia coli Yuliani, Ratna
Pharmacon Vol 12, No 2 (2011)
Publisher : Pharmacon

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Abstract

Jeruk purut (Citrus hystrix) merupakan tanaman dari suku Rutaceae yang telah lama dikenal masyarakat sebagai bahan cita rasa. Hasil penelitian sebelumnya melaporkan bahwa minyak atsiri jeruk nipis (Citrus aurantifolia) memiliki aktivitas antibakteri terhadap Staphylococcus aureus dan Escherichia coli. Penelitian ini bertujuan untuk mengukur aktivitas antibakteri minyak atsiri daun jeruk purut terhadap  Staphylococcus aureus dan Escherichia coli dan mendeteksi kandungan minyak atsiri yang mempunyai aktivitas antibakteri. Minyak atsiri daun jeruk purut yang diperoleh dengan cara destilasi uap dan air diuji aktivitas antibakterinya menggunakan metode dilusi cair. Kromatografi Lapis Tipis (KLT) dilakukan menggunakan silika gel GF254 dan heksan-etil asetat (9:1). Bercak hasil KLT dideteksi dengan UV254nm, UV366nm, anisaldehid-asam sulfat, dan vanilin-asam sulfat. Bioautografi dilakukan dengan metode kontak. Hasil uji aktivitas antibakteri menunjukkan bahwa minyak atsiri daun jeruk purut mempunyai aktivitas antibakteri terhadap Staphylococcus aureus dengan nilai Konsentrasi Hambat Minimum (KHM) dan Konsentrasi Bunuh Minimum (KBM) berturut-turut sebesar 1  dan 2%. Minyak atsiri juga mampu menghambat dan membunuh Escherichia coli dengan nilai KHM dan KBM £ 0,0625%. Hasil KLT menunjukkan bahwa minyak atsiri daun jeruk purut mengandung beberapa senyawa golongan terpen dengan Rf yang berbeda. Berdasarkan hasil bioautografi, salah satu senyawa golongan terpen tersebut mempunyai aktivitas antibakteri terhadap Staphylococcus aureus dan Escherichia coli.             Kata kunci : antibakteri, Citrus hystrix, E. coli, minyak atsiri, S. aureus
Skrining Aktivitas Antibakteri Ekstrak Etanol 70% Sepuluh Daun Tanaman Terhadap Klebsiella Pneumoniae Qolbi, Nurul; Yuliani, Ratna
Pharmacon: Jurnal Farmasi Indonesia Vol 15, No 1 (2018)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/pharmacon.v15i1.6169

Abstract

Klebsiella pneumoniae causes several diseases such as chronic obstructive lung disease and community pneumonia. The antibiotics used for treating the infectious diseases cause several side effects. Therefore, alternative medicines which have minimal side effects are needed. This research aimed to investigate antibacterial activity of ten ethanolic extract of leaves against K. pneumoniae, identify compounds and active compounds in extract which has the highest antibacterial activity. Compounds in leaves were extracted using 70% ethanol with maceration method. The disk diffusion method was used for screening antibacterial activity against K. pneumoniae. The identification of group of compounds in extract with the largest inhibitory zone diameter was done using Thin Layer Chromatography (TLC) and bioautographic test was performed to determine the active compounds. The results of antibacterial activity screening tests showed that all extracts have antibacterial activity except bamboo leaf extract. Breadfruit extract (Artocarpus altilis) has the highest antibacterial activity with diameter of inhibition zone of 18,33 ± 5,57 mm. Breadfruit extract contains tannin, polyphenol, alkaloid, terpenoid, saponin, and the active antibacterial compounds were terpenoid and saponin.
Uji Aktivitas Antibakteri Ekstrak Etanol Bawang Dayak (Eleutherine palmifolia (L.) Merr) Terhadap Bakteri Staphylococcus aureus Warsiti, Warsiti; Wardani, Sisca Dwikusuma; Ramadhan, Ardea Achmad; Yuliani, Ratna
Pharmacon: Jurnal Farmasi Indonesia Vol 15, No 2 (2018)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/pharmacon.v15i2.6526

Abstract

Staphylococcus aureus is an infectious bacteria with pus because of tissue damage. Infectious diseases caused by S. aureus include wound infections, ulcers, pimples, and impetigo. Dayak onions have the benefit of being antibacterial. This research aims to study the antibacterial activity of ethanolic extract of Dayak onions against  Staphylococcus aureus. The antibacterial study was carried out using the disk diffusion method. Identification of bacteria by Gram staining showed Gram-positive bacteria which can ferment mannitol with the mannitol test. A bacterial sensitivity test was conducted using three antibiotics i.e. cefoxitin 30μg, vancomycin 30μg, and linezolid 30μg. The bacteria was sensitive to all of the three antibiotics, with the inhibitory zone of 23.5 mm; 17 mm and 27.5 mm, respectively. Vancomycin 30μg was used as a positive control, resulted in average diameter of the inhibitory zone of 17.83  2.25 mm, whereas a control solvent DMSO (Dimethyl sulphoxide) did not produce zones. The ethanolic extract of Dayak onion in the concentration of 25%; 50%; 75% and 100% showed the average diameter of inhibitory zones of 8.17  0.29  mm; 10.67  1.44 mm; 10.33  1.53 mm; and 12.33  1.61  mm, respectively
AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAN FRAKSI KULIT BATANG BELIMBING WULUH (Averrhoa bilimbi Linn.) TERHADAP BAKTERI Klebsiella pneumoniae DAN Staphylococcus epidermidis BESERTA BIOAUTOGRAFINYA , MSi., Dr. Muhtadi; Ambarwati, Ria; Yuliani, Ratna
Biomedika Vol 4, No 2 (2012): Biomedika Agustus 2012
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v4i2.252

Abstract

Belimbing wuluh (Averrhoa bilimbi Linn.) is a tropical plant that has antibacterial properties. The purpose of this study was to test the antibacterial activity of bark Belimbing wuluh against Klebsiella pneumoniae and Staphylococcus epidermidis and their bioautography. Extraction methods used to research is method maceration with a solvent ethanol 96 %. Fractinations done by method partition liquid-liquid with a separating funnel. Test performed in this research covering identi? cation bacteria, the sensitivity bacteria, antibacterial activity, thin layer chromatography, bioautography. The result of antibacterial activity ethanol extract of disk diffusion method with concentrations 400 ?g/disk, 800 ?g/disk, 1600 ?g/disk is 8±0,5; 10,34±0,58; 12,17±0,76 on Klebsiella pneumoniae, 10,17±0,29; 11±0; 11.5±0 on Staphylococcus epidermidis, n-hexane fraction with concentration 400 ?g/disk, 800 ?g/disk, 1600 ?g/disk is 8,34±0,29; 9,34±0,29; 10,84±0,76 on Klebsialla pneumoniae, 8,5±0,5; 9,34±0,29; 10,67±0,29 on Staphylococcus epidermidis, ethyl acetate fraction with concentration 400 ?g/disk, 800 ?g/disk, 1600 ?g/disk is 9,17±0,29; 10,34±0,29; 11,17±0,29 on Klebsiella pneumoniae and 9,5±0,5; 10,67±0,29; 12,67±1,26 on Staphylococcus epidermidis, ethanol-water fractions with concentration 400 ?g/disk, 800 ?g/ disk, 1600 ?g/disk is 8,17±0,29; 9,17±0,29; 10±0 on Klebsiella pneumoniae, 9±0; 9,67±0,29; 10,34±0,29 on Staphylococcus epidermidis. The TLC show chemical compounds contained in the ethanol extract, n-heksan fraction, ethyl acetate fraction, and ethanol-water fraction is a compound of the saponins, alkaloids, ? avonoids and phenolic. Bioautography showed that ethanol extracts, n-heksan faction, ethyl acetate fraction, and etanol-airfaction Belimbing wuluh (Averrhoa bilimbi Linn.) bark have not antibacterial activity because there is no clear area around on plate TLC.Keywords: Belimbing wuluh (Averrhoa bilimbi Linn.), ethanol extract, fractination, antibacterial, bioautogra? .
EFEK ANTIINFLAMASI INFUSA RIMPANG TEMU PUTIH (Curcuma zedoaria (Berg) Roscoe) PADA TIKUS YANG DIINDUKSI KARAGENIN Sujono, Tanti Azizah; Patimah, Raudatul; Yuliani, Ratna
Biomedika Vol 4, No 2 (2012): Biomedika Agustus 2012
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v4i2.253

Abstract

In-flammation is the body’s reaction to foreign substances that enter the body with signs of redness, heat, swelling, pain, and impaired organ functions. Curcuma rhizome including medicinal plants that have properties relieve the pain and inflammation of the skin. The purpose of this research was to examine the anti-iflammation effect of Curcuma zedoaria (Berg) Roscoe Rhizome infusion on carrageenan induced paw edema in rats. This research used experimental method with completely randomized design. Twenty five healthy Wistar male rats, 2-3 months old and 150-250 g divided into five groups and each group consist of 5 rats. Rats in group I that served as negative control were given 2.5 mL/200gbw of aquadest. Rats in group II as positive control were given sodium diclofenac with dose of 6.75 mg/kgbw. Group III, IV, and V were given Curcuma zedoaria (Berg) Roscoe Rhizome infusion with dose of 0.625, 1.250 and 2.500 g/kgbw, respectively. Treatments were given per oral 1 hour before injection of 0.1 ml carrageenan 1% subplantar. Rat paw edema volume was measured before and after carrageenan injection. The measurement was done every 0.5 hour for 6 hours observation. Area Under the Curve (AUC) that is calculated from paw edema volume data, was use to calculate percentage of anti-inflammatory effect. Data were analyzed with one way Anova and Least Signifi cant Difference with confi dence level 95%. Infusion of Curcuma zedoaria Rhizome with doses of 0,625, 1,250, and 2,500 g/kgbw had anti-inflammatory effect in Wistar male rats which were induced by Carrageenan 1%. Percentage of anti-inflammation effect (44,16+5,11)%, (48,70+7,05)%, (59,09+9,61)% respectively and the effects were equivalent with positive control.Key words: Anti-inflammation, Curcuma zedoaria (Berg) Roscoe, infusion, Carrageenan.
AKTIVITAS ANTIBAKTERI MINYAK ATSIRI DAUN JERUK PURUT (Citrus hystrix) TERHADAP Staphylococcus aureus DAN Escherichia coli Yuliani, Ratna
Pharmacon: Jurnal Farmasi Indonesia Vol 12, No 2 (2011)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/pharmacon.v12i2.31

Abstract

Jeruk purut (Citrus hystrix) merupakan tanaman dari suku Rutaceae yang telah lama dikenal masyarakat sebagai bahan cita rasa. Hasil penelitian sebelumnya melaporkan bahwa minyak atsiri jeruk nipis (Citrus aurantifolia) memiliki aktivitas antibakteri terhadap Staphylococcus aureus dan Escherichia coli. Penelitian ini bertujuan untuk mengukur aktivitas antibakteri minyak atsiri daun jeruk purut terhadap  Staphylococcus aureus dan Escherichia coli dan mendeteksi kandungan minyak atsiri yang mempunyai aktivitas antibakteri. Minyak atsiri daun jeruk purut yang diperoleh dengan cara destilasi uap dan air diuji aktivitas antibakterinya menggunakan metode dilusi cair. Kromatografi Lapis Tipis (KLT) dilakukan menggunakan silika gel GF254 dan heksan-etil asetat (9:1). Bercak hasil KLT dideteksi dengan UV254nm, UV366nm, anisaldehid-asam sulfat, dan vanilin-asam sulfat. Bioautografi dilakukan dengan metode kontak. Hasil uji aktivitas antibakteri menunjukkan bahwa minyak atsiri daun jeruk purut mempunyai aktivitas antibakteri terhadap Staphylococcus aureus dengan nilai Konsentrasi Hambat Minimum (KHM) dan Konsentrasi Bunuh Minimum (KBM) berturut-turut sebesar 1  dan 2%. Minyak atsiri juga mampu menghambat dan membunuh Escherichia coli dengan nilai KHM dan KBM £ 0,0625%. Hasil KLT menunjukkan bahwa minyak atsiri daun jeruk purut mengandung beberapa senyawa golongan terpen dengan Rf yang berbeda. Berdasarkan hasil bioautografi, salah satu senyawa golongan terpen tersebut mempunyai aktivitas antibakteri terhadap Staphylococcus aureus dan Escherichia coli.             Kata kunci : antibakteri, Citrus hystrix, E. coli, minyak atsiri, S. aureus
HASIL SKRINING AKTIVITAS SITOTOKSIK EKSTRAK ETANOL DAUN KELENGKENG (DIMOCARPUS LONGAN), DAUN KERSEN (MUNTINGIA CALABURA), DAN DAUN ALPUKAT (PERSEA AMERICANA) TERHADAP SEL T47D DAN WIDR Yuliani, Ratna; Santoso, Broto; Permatasani, Bella; Sari, Diah Mukti
Pharmacon: Jurnal Farmasi Indonesia Vol 16, No 2 (2019)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/pharmacon.v16i2.9050

Abstract

Cancer treatments usually cause adverse drug reactions. Therefore, safe anticancer drugs are needed in the treatment of cancer. One source of medicine that can be explored is plant. Extracts of longan leaves (Dimocarpus longan), jamaican cherry leaves (Muntingia calabura), and avocado leaves (Persea americana) have been tested for cytotoxic activity against several cancer cell lines. This study aims to determine the cytotoxic activity of ethanolic extract of longan leaves, jamaican cherry leaves, and avocado leaves against T47D and WiDr cells and to identify secondary metabolites in the extracts which have the highest activity. Ethanolic extract of longan leaves, jamaican cherry leaves, and avocado leaves were tested for their cytotoxic activity using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. Identification of secondary metabolites in the ethanolic extract of avocado leaves was carried out by thin layer chromatography method using silica gel GF254 as the stationary phase and a mixture of n-hexane and acetone (6:4) as the mobile phase. Cytotoxic test results show that ethanolic extract of longan leaves and cherry leaves with concentration of up to 1600 ?g/mL do not reduce the T47D and WiDr living cells to 50%. Avocado leaf extract decreases the percentage of living T47D cells and WiDr with IC50 values of  790.679 µg/mL and 1072.2 µg/mL, respectively. The ethanolic extract of avocado leaves contains flavonoid, phenolic, and terpenoid. Ethanolic extract of longan leaves, cherry leaves and avocado leaves do not have cytotoxic activity against T47D and WiDr cells.
ETHANOLIC EXTRACT OF PAPAYA LEAVES (CARICA PAPAYA) AND ITS FRACTIONS HAVE NO POTENTIAL CYTOTOXICITY ON T47D CELLS Yuliani, Ratna; Syahdeni, Faisal
Pharmacon: Jurnal Farmasi Indonesia Vol 17, No 1 (2020)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/pharmacon.v17i1.10760

Abstract

Breast cancer is one type of cancer that causes high mortality rate in women. Plants produce secondary metabolites with a wide range of activity, one of which is as an anticancer. This research was conducted to investigate the cytotoxic effect of ethanolic extract of papaya leaves (Carica papaya) and its fractions on T47D cell line. Extraction of the natural compounds from papaya leaves was carried out by soaking the leaf powder in 96% ethanol followed by filtration and solvent evaporation. Liquid partition was done to obtain hexane, ethyl acetate, and water fractions. The cytotoxicity of the extract and the fractions was tested using MTT assay. Identification of chemical compounds in the ethyl acetate fraction was conducted using a thin layer chromatography method that used silica gel GF 254 as the stationary phase and acetone and hexane (4:6) as the mobile phase. The MTT assay result shows that the extract does not inhibit the growth of T47D cells. The viability of T47D cells can be reduced by the hexane, ethyl acetate, and water fractions with IC50 values of 2,231.30, 557.33, and 2,112.81 ?g/mL, respectively. The ethyl acetate fraction contains flavonoids, alkaloids, phenolics, and terpenoids. The ethanolic extract of papaya leaves and the fractions has no potential cytotoxicity on T47D cells due to high IC50 values.
Co-Authors Annisa Wifa Rizqi Madjid Ardea Achmad Ramadhan Bella Permatasani Broto Santoso Cut Zahri Harun Diah Mukti Sari Dimas Satrio Utomo Diva Ratna Shabrina Dr. Muhtadi , MSi., Dr. Muhtadi Faisal Syahdeni Monarita Puspita Dewi Mustaqim Alfarizky Naufal Farras Nurul Qolbi Permatasani, Bella Qolbi, Nurul Ramadhan, Ardea Achmad Raudatul Patimah, Raudatul Ria Ambarwati Rizkiananda Wardani Sari, Diah Mukti Sisca Dwikusuma Wardani Syahdeni, Faisal Tanti Azizah Sujono Wardani, Sisca Dwikusuma Warsiti Warsiti Warsiti Warsiti Y Yuliansah Yusrizal Yusrizal