Wagiyana Wagiyana, Wagiyana
Unknown Affiliation

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

THE UTILIZATION OF ZEOLITE AS MATERIAL PRODUCTION OF BIOPESTICIDE ACTIVE NEMATODA STEINERNEMA SPP. WITH GRANULAR SHAPED Setyobudi, Bambang; Wagiyana, Wagiyana
Jurnal Zeolit Indonesia Vol 7, No 2 (2008)
Publisher : Jurnal Zeolit Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (48.502 KB)

Abstract

The study about biopesticide granular shaped has been conducted by utilizing zeolite and vertisol. Both materials are used as media that functions to storage Steinernema. The purpose of this study was to find method and formulas to produce biopesticide active nematodes with granular shaped that have high viability and effectiveness and can last long live. The result showed that drop method on combination of zeolite and vertisol material produce granule with ± 8 mm diameter and weight of ± 0.3 g. Biopesticide on granule shape that mixture result of zeolite, vertisol, and Steinernema spp. able to maintain viability of Steinernema spp. until nine weeks. The formulas to produce granule biopesticide an effective until nine weeks is a combinations of mixture 50% up to 75% zeolite on vertisol.
MASS PRODUCTION OF ENTOMOPATHOGENIC NEMATODES OF LOCAL ISOLATES AS BIOLOGICAL CONTROL AGENTS OF COFFEE BERRY BORER (HYPOTHENEMUS HAMPEI FERR.) Wagiyana, Wagiyana; Sulistyanto, Didik; Waluyo, Joko
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 19, No 1 (2019): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j.hptt.1198-14

Abstract

Entomopathogenic Nematodes (EpNs) that serve as biological control agents include Steinernema spp and  Heterorhabditis spp. EPNs Heterorhabditis indicus (Bromo Isolate) has a high toxicity against larvae Lepidoptera and Coleoptera and was successfully developed for mass production in Biological Control Laboratory of Jember University. These nematodes will be formulated as solid and liquid biopesticides. This research aims: to find EPNs local isolates from smallholder coffee plantations; to identify local and in vivo and in vitro cultured EPNs isolates; to determine the pathogenicity of EpNs local isolate against Coffee Berry Borrer (CBB) Hypothenemus hampei Ferr and to formulate EpNs in vitro culture as a liquid and solid formula. These formula were tested for the patogenicity and viability of EpNs to the larvae of H. hampei, Tenebrio molitor and Galleria melonella. The results showed that the pathogenecity of EpNs isolate to the larvae of CBB in Silo was 30% after 24 hours and 90% after 48 hours of in vivo inoculation. However, the mortality of CBB larvae was only 10% by liquid spraying on the coffee berry. The viability was 524 IJ (Infective Juvenile) on liquid formula packed on polyurethane sponge, and this was higher than that on solid formula (330 IJ).