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All Journal Jurnal Sain Veteriner Jurnal Veteriner Jurnal Ilmiah Perikanan dan Kelautan Indonesian Journal of Tropical and Infectious Disease Aceh Journal of Animal Science Biosaintifika: Journal of Biology & Biology Education Jurnal Medik Veteriner Journal of Aquaculture and Fish Health Veterinary Biomedical and Clinical Journal Journal of Parasite Science journal of Basic Medical Veterinary Jurnal Kedokteran Hewan

Setiawan Koesdarto

Departemen Parasitologi Veteriner, Universitas Airlangga, Surabaya

Author-ID : 515120

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Evaluation of Salmonella sp Contamination and its Antibiotics Resistance Patterns Isolated from Broiler Meat Sold at Wet Market in Center of Surabaya Aprillian, Risky; Rahardjo, Dadik; Koesdarto, Setiawan
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 6 (2015)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar

Antibiotic resistance now days become a main issue to the medical researches as found many positive result of antibiotic resistance test. One of the causes of antibiotic resistance is using antibiotic as a feed additive to animal. Bacteria that are resistant to antibiotics can be a danger to humans, in this case the resistant bacteria as a result of treatment errors animals, especially chickens that uses low-dose antibiotics as growth promoters. This study aimed to determine the contamination of Salmonella sp and its antibiotics resistance patterns of Salmonella sp isolated from broiler meat sold at wet market in the Center of Surabaya: (a) Pasar Kembang, (b) Pasar Kupang, (c) Pasar Dukuh Kupang, (d) Pasar Kedungsari, (e) Pasar Kedungdoro and (f) Pasar Keputran. The method that used in this study was bacteriological isolation and identification method. The method started with pre-enrichment using Buffered Pepton Water, selective enrichment using Tetrathionate Broth and Selenite Cysteine broth, selective media using Salmonella-Shigella Agar, Biochemical test using Triple Sugar Iron Agar, Simon Citrate, Methyl Red â Voges Proskauer, and Sulfide Indol Motility, and followed with susceptibility test according to Kirby-Bauer method using Mueller-Hinton Agar. The antibiotics that used in susceptibility test were: (a) Meropenem, (b) Ampicillin Sulbactam, (c) Amikacin, (d) Ofloxacin and (e) Nalidixic Acid. The results of this study were found 90% or 27 of 30 samples positive contaminated with Salmonella sp. The results of antibiotics resistance from 27 isolates 0% were resistant to Meropenem, 0% were resistant to Amikacin; 3.7% were resistant to Ampicillin-Sulbactam; 11.1% were resistant to Ofloxacin and 44.4% were resistant to Nalidixic Acid.

Study on the Morphology of Fasciola gigantica and Economic Losses due to Fasciolosis in Berau, East Kalimantan Prasetya, Muhammad Rofi'; Koesdarto, Setiawan; Lastuti, Nunuk Dyah Retno; Suwanti, Lucia Tri; Kusnoto, Kusnoto; Yunus, Muchammad
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Fasciolosis is one of the endemic parasitic diseases in Indonesia and causes significant economic losses. Fasciola gigantica is the main species found to infect livestock. This research aimed to describe the morphology of F. gigantica histologically using hematoxylin and eosin staining as well as ultrastructure morphology using scanning electron microscopy (SEM). Besides, the study was also carried out to determine the prevalence and estimation of economic losses due to fasciolosis in Berau Regency. The histological identification of flukes morphology found several organs including oral sucker (OS), ventral sucker (VS), cirrus (C), genital pore (GP), uterus (U), ovary (O), tegument (Te), testis (T), and caecum (C). In this study, ultrastructure morphology was divided into anterior and midbody region. In the anterior region, there are an oral sucker (OS) with a diameter of 304.5 µm and a ventral sucker (VS) with a diameter of 571 µm. It was also found the anterior spine with a width of 25.4 µm and a height of 22.8 µm. In the midbody, the spine has a width of ± 26.7 µm and a length of ± 38.9 µm leading posteriorly. Postmortem examination results showed that the prevalence of fasciolosis in Berau regency was 21.65% or 34 of 157 cows which were positive with an estimated loss of 228,656,475.00 IDR/16,450 USD (1 USD=13,900 IDR). The amount of loss due to fasciolosis must be a particular concern in carrying out control and prevention measures. Histology and ultrastructure morphology can become a reference in the development of anthelmintic testing that has been used so far in Indonesia.

KORELASI ANTARA PADAT TEBAR DENGAN INFESTASI EKTOPARASIT PADA UDANG VANAME (Litopenaeus vannamei) DI TAMBAK SUPER INTENSIF Adiacahya, Eren; Koesdarto, Setiawan; Mahasri, Gunanti
Jurnal Sain Veteriner Vol 38, No 2 (2020): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.54894

Teknologi budidaya udang yang sudah banyak digunakan di pertambakan di Indonesia, adalah teknologi budidaya yang menggunakan pola super intensif, dengan menggunakan padat tebar yang tinggi, yaitu lebih dari 150 ekor/meter persegi. Padat tebar yang tinggi tersebut dapat mengakibatkan kondisi air sebagai media mengalami penurunan, sehingga menyebabkan udang mengalami stres dan penurunan pertahanan tubuh, sehingga udang akan mudah terserang penyakit. Tujuan dari penelitian ini adalah untuk mengetahui korelasi antara padat tebar dengan infestasi ektoparasit protozoa pada udang vaname (Litopenaeus vannamei) pada tambak super intensif. Penelitian ini merupakan penelitian Survei, dengan Rancangan Penelitian Cross Sectional Study, pengambilan sampel menggunakan metode proporsive sampling, yang dilakukan di daerah pertambakan di Kabupaten Tuban. Sampel udang yang diambil masing-masing sebanyak 50 ekor dari 3 petak tambak dengan padat tebar 150, 200 dan 300 ekor/m2. Hasil penelitian menunjukkan bahwa ektoparasit yang ditemukan pada udang vaname yang dipelihara pada tambak dengan padat tebar tinggi (super intensif) adalah Zoothamnium, Epistylis dan Vorticella, dengan intensitas berturut-turut sebesar 278,32 ; 391,34 dan 466,02 dengan derajat infestasi berat, pada semua udang yang dipelihara baik pada padat tebar 150, 200 dan 300 ekor/m2. Tidak ada korelasi antara padat tebar dengan infestasi ektoparasit pada udang vaname pada tambak super intensif, dengan nilai R = 0,394, adanya peningkatan padat tebar tidak diiringi dengan infestasi ketiga genus ektoparasit tersebut.

Karakterisasi Protein VirB4 Brucella abortus Isolat Lokal dengan Teknik Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis Ratih Novita Praja; Didik Handijatno; Setiawan Koesdarto; Aditya Yudhana
Jurnal Veteriner Vol 18 No 3 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Brucellosis is a zoonotic disease cause by pathogenic bacteria of the genus Brucella. The disease infects livestock mammals such as cows, goats, pigs, and including humans. Brucella abortus has several potential virulence factors, i.e. Proteins VirB. Type IV secretion system (T4SS) which is a combination of 12 proteins from VirB1-VirB11 and VirD4. Brucella can survive for long periods in the environment despite the limitations of nutrients and oxygen. This study aims to characterize the protein VirB4 of local isolate of B. abortus using SDS PAGE (Sodium Dodecly sulfate-polyacrylamide gel electrophoresis). The results showed that the protein contained 10 protein bands with a molecular weight of 158.93; 110.89; 99.931; 70.60; 64.61; 59.25; 45.32; 42.35; 23.63; and 16.70 kDa, respectively. Protein VirB4 of the local isolate of B. abortus have a molecular weight of 59.25 kDa. ABSTRAK Brucellosis merupakan salah satu penyakit zoonosis yang disebabkan oleh bakteri patogen genus Brucella. Penyakit ini menyerang hewan ternak mamalia seperti sapi, kambing, babi, dan dapat menular ke manusia. Bakteri Brucella abortus memiliki faktor virulensi potensial yaitu protein VirB. Type IV Secretion System (T4SS) merupakan gabungan dari 12 protein yaitu VirB1–VirB11 dan VirD4. Brucella dapat bertahan hidup lama di dalam lingkungan meskipun memiliki keterbatasan nutrisi dan oksigen. Penelitian ini dilakukan untuk karakterisasi protein VirB4 B. abortus isolat lokal dengan metode Sodium Dodecly Sulfate-Polyacrylamide Gel Electrophoresis (SDS PAGE). Hasil karakterisasi protein B. abortus isolat lokal dengan teknik SDS-PAGE terdapat 10 pita protein dengan bobot molekul 158,93; 110,89; 99,931; 70,60; 64,61; 59,25; 45,32; 42,35; 23,63; dan 16,70 kDa. Simpulan penelitian ini adalah terdapat protein VirB4 B. abortus isolat lokal yang mempunyai bobot molekul 59,25 kDa.

Perbedaan Larva Stadium Kedua dan L2 Toxocara canis pada Jaringan Mencit Menggunakan Scanning Electron Microscopy (THE DIFFERENCES BETWEEN SECOND STAGE LARVAE AND L2 TOXOCARA CANIS ON MICE TISSUE BY USING SCANNING ELECTRON MICROSCOPY) Vindo Rossy Pertiwi; Kusnoto Kusnoto; Setiawan Koesdarto; Nunuk Dyah Retno Lastuti; Lucia Tri Suwanti; Mufasirin Mufasirin
Jurnal Veteriner Vol 20 No 3 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Toxocariasis is one of zoonosis diseases that caused by Toxocara spp. that is Toxocara canis. Toxocara canis has several stages until it can infect animals and humans, namely the egg stage, larvae first stage (L1), larvae second stage (L2), larvae third stage (L3) to adult worms. Studies about the L2 and L2 tissue of T. canis found in paratenic hosts using Scanning Electron Microscopy (SEM) have not been widely performed. Some of the causes include L2 being not easily to found and identified, so research rarely raises the ultrastructural morphology of L2 and L2 tissues. Knowledge about the ultrastructural morphology of L2 and L2 tissue of T. canis worms is very important to determining the diagnosis, especially the etiological diagnosis. The purpose of this study is to detected morphology of L2 and L2 tissues of T. canis using SEM. Samples from this study is faeces of dogs that infected with toxocariasis and the digestive tract of dogs obtained from dog slaughter houses. The sample is an adult worm of T. canis; the female worm is dissected and taken uterus to collect worm eggs. The results of this study on microscopic and optilab examination showed a difference between L2 and L2 tissue that the length of L2 hatched from embryonic eggs was 390 ìm and with a width of 23.4 ìm at the midpoint of the body. Larvae second stage length from the infected somatic tissue is 410 ìm and the width is 22.5 ìm at the midpoint, and then difference in dorsal lip, cuticles, body ring, cervical alae, buccal capsul, tail.

Morfometri dan Ultrastruktur Cacing Fasciola gigantica pada Sapi Donggala dan Sapi Bali di Kabupaten Berau, Kalimantan Timur (MORPHOMETRY AND ULTRASTRUCTURAL OF FASCIOLA GIGANTICA WORM IN DONGGALA AND BALI CATTLE IN BERAU DISTRICT, EAST KALIMANTAN) Muhammad Rofi' Prasetya; Nunuk Dyah Retno Lastuti; Lucia Tri Suwanti; Setiawan Koesdarto; Kusnoto Kusnoto; Muchammad Yunus
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Fasciolosis is one of the endemic parasitic diseases in Indonesia. Fasciola gigantica is the main species found to infect livestock, especially beef cattle. The purpose of this study was to analyze the morphometry and identify the ultrastructure morphology of the F. gigantica worm isolated from beef cattle in Berau abattoir. The F. gigantica worms were isolated from two cattle breeds, namely Donggala cattle and Bali cattle. Worm was stained by using carmine methode to analyze the morphometric of the worm and scanning electron microscopy to identify ultrastructure morphology. Morphometric of the worm was analysed with the one sample t-test and multidimensional scaling statistical methods. The results of the morphometric analysis showed that F. gigantica from both breeds had significant differences (P<0.05) between the parameters and based on multidimensional scaling analysis had similarities with F. intermedia from Iran. Based on scanning electron microscopy examination showed that F. gigantica from the Donggala cattle breed was infected with type 1 of F. gigantica while F. gigantica from the Bali cattle breed was infected with type 2 of F. gigantica based on ventral sucker, oral sucker, and spine. It can be concluded that F. gigantica found in beef cattle in Berau was type 1 F. gigantica (Donggala cattlebreed) and type 2 F. gigantica (Bali cattle breed) and similar with F. intermedia from Iran.

Blastocystis sp. : Evaluation of polyclonal antibody prepared from crude protein for serological diagnosis using Rabbit serum Septian Hakim Susantoputro; Lucia Tri Suwanti; Rahadju Ernawati; Mufasirin Mufasirin; Setiawan Koesdarto; Wiwiek Tyasningsih; Heni Puspitasari
Aceh Journal of Animal Science Vol 5, No 2 (2020): Desember 2020
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/ajas.5.2.16780

The diagnosis of Blastocystis infection is still based on the clinical sign which is not specific and there is no available serologic test for it. This study aimed to evaluate the polyclonal antibody prepared form crude protein of Blastocystis for the development of the Blastocystis serological test. Crude protein was extracted from the yeast of Blastocystis sp, then inoculated into rabbits to produce the antibody of crude protein. The serum of rabbits would be collected before and after immunization to compare the antibody titer. The profile of crude protein was analyzed using SDS-Page. The rabbit serum was analyzed using ELISA and Western Blot. The SDS-Page result showed bands in 100 kDa, 90 kDa, 70 kDa, 60 kDa, 58 kDa, 50 kDa, 40 kDa, 35 kDa, 30 kDa and 27 kDa. The ELISA assay showed that there was an increase in antibody titer of crude protein after immunization. Western Blot showed that three proteins (30 kDa, 40 kDa and 50 kDa) having immunogenicity characteristic. It is concluded that protein 30 kDa, 40 kDa and 50 kDa prepared from the crude protein of Blastocystis sp. can be used for developing a serologic test for Blastocystis infection. Keywords: Blastocystis sp, Crude Protein, Polyclonal Antibody .

Perubahan Patologi Kulit Ikan Gurami (Osphronemu gouramy) Akibat Infestasi Lernaea cyprinacea Pada Derajat Infestasi Yang Berbeda [The Pathology Alteration Of Gouramy (Osphronemus gouramy) Integument Infested By Lernaea cyprinacea At Different Degress Of Infestation] Gunanti Mahasri; Putri Desi Wulan Sari; Setiawan Koesdarto
Jurnal Ilmiah Perikanan dan Kelautan Vol. 2 No. 2 (2010): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Abstract The development of fish consumption in Indonesia was promising increasingly and growing very rapidly. One of the priority commodities in the development of aquaculture subsector was the gourami (Osphronemus gouramy). Many factors must be faced in the cultivation of gouramy, including the problem of fish diseases caused by Lernaea cyprinacea. The aim of this research is to find out the description of anatomic pathology and histopathology of gouramy which infested by L. cyprinacea at different degrees of infestation. This research uses descriptive method. The research showed that the gouramy integument which infested by L. cyprinacea at low, moderate and heavy infestation cause moderate until heavy defect with 2.48; 2.42 and 2.32 in reaverage scoring. Hence, accordance with pathological anatomy, L. cyprinacea infestation caused necrotic nodules, lesion which covered by exudate and haemorrhage. While the histopathology of the integument changes found were inflammatory cell infiltration, congestion and haemorrhage in the low, moderate and heavy infestation.

EVALUATION OF Salmonella sp CONTAMINATION AND ITS ANTIBIOTICS RESISTANCE PATTERNS ISOLATED FROM BROILER MEAT SOLD AT WET MARKET IN CENTER OF SURABAYA Risky Aprillian; Dadik Rahardjo; Setiawan Koesdarto
Indonesian Journal of Tropical and Infectious Disease Vol. 5 No. 6 (2015)
Publisher : Institute of Topical Disease Universitas Airlangga

Antibiotic resistance now days become a main issue to the medical researches as found many positive result of antibiotic resistance test. One of the causes of antibiotic resistance is using antibiotic as a feed additive to animal. Bacteria that are resistant to antibiotics can be a danger to humans, in this case the resistant bacteria as a result of treatment errors animals, especially chickens that uses low-dose antibiotics as growth promoters. This study aimed to determine the contamination of Salmonella sp and its antibiotics resistance patterns of Salmonella sp isolated from broiler meat sold at wet market in the Center of Surabaya: (a) Pasar Kembang, (b) Pasar Kupang, (c) Pasar Dukuh Kupang, (d) Pasar Kedungsari, (e) Pasar Kedungdoro and (f) Pasar Keputran. The method that used in this study was bacteriological isolation and identification method. The method started with pre-enrichment using Buffered Pepton Water, selective enrichment using Tetrathionate Broth and Selenite Cysteine broth, selective media using Salmonella-Shigella Agar, Biochemical test using Triple Sugar Iron Agar, Simon Citrate, Methyl Red – Voges Proskauer, and Sulfide Indol Motility, and followed with susceptibility test according to Kirby-Bauer method using Mueller-Hinton Agar. The antibiotics that used in susceptibility test were: (a) Meropenem, (b) Ampicillin Sulbactam, (c) Amikacin, (d) Ofloxacin and (e) Nalidixic Acid. The results of this study were found 90% or 27 of 30 samples positive contaminated with Salmonella sp. The results of antibiotics resistance from 27 isolates 0% were resistant to Meropenem, 0% were resistant to Amikacin; 3.7% were resistant to Ampicillin-Sulbactam; 11.1% were resistant to Ofloxacin and 44.4% were resistant to Nalidixic Acid.

THE IMMUNOSTIMULAN POTENTIAL OF TENGGULUN (PROTIUM JAVANICUM) LEAVES TOWARDS T CELL CD4+ AND IFNγ SECRETION ON PBMC CHICKEN Andi Jayawardhana; Heni Puspitasari; Dewa Ketut Meles; Setiawan Koesdarto
Indonesian Journal of Tropical and Infectious Disease Vol. 6 No. 3 (2016)
Publisher : Institute of Topical Disease Universitas Airlangga

One of the plants with immunostimulant activity is Tenggulun leaves which contain of flavonoid, like terpenoid. The aim of this research is to find the potential of Tenggulun’s leaves extract to have the immunostimulant activities. The potential of immunostimulant activity is identified by the increasing the amount of T-cell CD4+ expression and IFNγ secretion. The research method is conducted through cultured chicken PBMC which is infected by ND virus; it is then treated with Tenggulun’s leaves extract with immunostimulant. The result of immunocitochemistry examination CD4+ secretion on PBMC cultures shows how tenggulun is significantly different from the control in the secretion CD4+. The 10μg of tenggulun extract can modulate the T cell CD4+ secretion 68.8±0.83. It is significantly different from K (control) (p<0,05) and treatment group K+, P0, and P1. The examination of IFNγ level using ELISA from tenggulun leaves extract of 10μg doses were inoculated after being infected by ND virus contained immonostimulant potential in increasing the secretion of IFNγ 120.91±6.44. It is significantly different from K-, K+, and P1, yet not significantly different from P0. The content of terpenoid can increase IFNγ secretion on the macrofag cells culture and limfosit cells.

Co-Authors Adiacahya, Eren Adiana Mutamsari Witaningrum Aditya Yudhana Agnes Theresia Soelih Estoepangestie Agus Sunarso Agus Wijaya Akbar Haryo Widhi Trismiharto Akyun Rozaqi Syah Putra Aldi Hamdani Amelia Dwita Safitri Ana Amaliah Andi Jayawardhana Anindita Khairunnisa Annisa Novia Damayanti Arif Pratiwi Arum Puspitasari Bambang Sektiari Lukiswanto Boedi Setiawan Boedi Setiawan Bryan Ahmad Affan Lubis Chairul Anwar Nidom Dadik Rahardjo Dadik Rahardjo, Dadik Dewi Mariyam Dhandy Koesoemo Wardhana Dhimar Maulud Dyahningrum Didik Handijatno Didik Handijatno Djoko Poetranto Dyah Ayu Kurniawati E Djoko Poetranto Eka Pramyrtha Hestinah Emy Koestanti Sabdoningrum Endang Suprihati Eny Coolfina Simarmata Felita Widyaningsih Firman Hadi Fanani Gunanti Mahasri Hana Eliyani Hani Plumeriastuti Heni Puspitasari Heni Puspitasari Herry Agoes Hermadi Ida Yuniarti Indah Norma Triana Inggarsetya Syah Audini Ira Sari Yudaniayanti Iwan Sahrial Hamid Iwan Sahrial Hamid Jessica Giovani Khalissa Farah Alifia Kismiyati , Koesnoto Supranianondo Koesnoto Supriandono Kuncoro Puguh Santoso Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Lutfiah Annisa Billa Mas’ud Hariadi Melani Anggraini Melanie Aulia Ashfiyah Meles, Dewa Ketut Mirny Lamid Mochamad Lazuardi Mohamad Safri Sauqi Muchammad Yunus Mufasirin Muhammad Rofi' Prasetya Mustofa Helmi Effendi Nenny Harijani Ngakan Made Rai Widjaja Nila Murodah Nisa’ Rachmaningtyas Putri Novia Intan Kurnia Nunuk Dyah Retno Lastuti Nunuk Dyah Retno Lastuti Ogen Sea Poedji Hastutiek Prasetya, Muhammad Rofi' Primarizky, Hardany Rahadju Ernawati Rahaju Ernawati Rahmi Sugihartuti Rahmi Sugihartuti Rari Noviana Ratih Novita Praja Ratih Prajnya Paramitha Rimayanti Rimayanti Risky Aprillian Risky Aprillian, Risky Rochmah Kurniasanti Sari, Putri Desi Wulan Sarmanu Sarmanu Septian Hakim Susantoputro Sesa Puput Febriyanti Setiawati Sigit Soeharsono Soeharsono Sri Agus Sudjarwo Sri Mumpuni Sosiawati Sri Mumpuni Sosiawati Sunarso, Agus Suryo Kuncorojakti Suwarno Suwarno Suzanita Utama Tjuk Imam Restiadi Trifena Pristi Anindyta Tyasningsih, Wiwiek Vanna Lidya Kharisma Vindo Rossy Pertiwi Wiwik Tyasningsih Yeni Dhamayanti Yulianna Puspitasari Yunus, Muchammad Zhaza Afililla Zhaza Afililla

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