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All Journal Medical Journal of Indonesia Acta Biochimica Indonesiana Jurnal Biomedika dan Kesehatan Majalah Kedokteran Neurosains World Nutrition Journal Muhammadiyah Journal of Geriatric Indonesian Journal of Medical Chemistry and Bioinformatics
Ninik Mudjihartini
Department Of Biochemistry And Molecular Biology, Faculty Of Medicine, Universitas Indonesia, Jakarta, Indonesia
Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Additional benefit of higher dose green tea in lowering postprandial blood glucose Lahirin, Rita; Permadhi, Inge; Mudjihatini, Ninik; Ridwan, Rahmawaty; Sugianto, Ray
Medical Journal of Indonesia Vol 24, No 2 (2015): June
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (414.177 KB) | DOI: 10.13181/mji.v24i2.1167

Abstract

Background: Green tea contains catechins that have inhibitory effects on amylase, sucrase, and sodium-dependent glucose transporter (SGLT) which result in lowering of postprandial blood glucose (PBG). This beneficial effect has been widely demonstrated using the usual dose (UD) of green tea preparation. Our study was aimed to explore futher lowering of PBG using high dose (HD) of green tea in healthy adolescents.Methods: 24 subjects received 100 mL infusion of either 0.67 or 3.33 grams of green tea with test meal. Fasting, PBG at 30, 60, 120 minutes were measured. Subjects were cross-overed after wash out. PBG and its incremental area under the curve (IAUC) difference between groups were analyzed with paired T-test. Cathecin contents of tea were measured using high-performance liquid chromatography (HPLC).Results: The PBG of HD group was lower compared to UD (at 60 minutes =113.70 ± 13.20 vs 124.16 ± 8.17 mg/dL, p = 0.005; at 120 minutes = 88.95 ± 6.13 vs 105.25 ± 13.85 mg/dL, p < 0.001). The IAUC of HD was also found to be lower compared to UD (2055.0 vs 3411.9 min.mg/dL, p < 0.001).Conclusion: Additional benefit of lowering PBG can be achieved by using higher dose of green tea. This study recommends preparing higher dose of green tea drinks for better control of PBG.
Altered expressions of endothelial junction protein of placental capillaries in premature infants with intraventricular hemorrhage Ekawati, Maria; Mujihartini, Ninik; Jusuf, Ahmad A.; Dharmasetiawani, Nani; Jusman, Sri W.A.; Sadikin, Mohamad
Medical Journal of Indonesia Vol 25, No 3 (2016): September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (691.713 KB) | DOI: 10.13181/mji.v25i3.1287

Abstract

Background: Placental hypoxia may lead to oxidative stress, which inflicts damage to capillary protein junction. The aim of this study was to evaluate altered expression of endothelial junction protein of capillaries in hypoxia condition and to observe its correlation with the incidence of  intraventricular hemorrhage in premature infants.Methods: A cross-sectional study was conducted by using placental tissues of premature infants as amodel of capillary integrity (29 hypoxic and 29 non-hypoxic). Hypoxia inducible factor (HIF)-1α was measured to define placental tissue response to hypoxia; malondialdehyde (MDA) and glutathione (GSH) served as markers of oxidative stress. The expressions of junctional proteins, N-cadherin and occludin were analyzed by immunohistochemistry. Intraventricular hemorrhage (IVH) was detected by cranial ultrasound at the third day. Unpaired t test, Mann-Whitney, and Chi-square tests were used to analyze the data.Results: The HIF-1α and MDA levels were slightly, but not significantly, higher in hypoxia group {13.64±8.70 pg/mg protein and 10.31 pmol/mg tissue (ranged 1.92–93.61), respectively}  compared to non- hypoxia group {10.65±5.35 pg/mg protein and 9.77 pmol/mg tissue (ranged 2.42–93.31)}. GSH levels were not different in both groups (38.14 (ranged 9.44–118.91) and  38.47(ranged 16.49–126.76) ng/mg protein, respectively. mRNA expression of N-cadherin (0.13) and occludin (0.096) were significantly lower in hypoxia comparedto non-hypoxia group (p=0,001), while protein expression of  N-cadherin (3.4; 75.9; 6.9; 13.8%) and occludin  (20.7; 3.4; 69.0; 3.4; 6.9%)  in hypoxia group was not associated with IVH (p=0.783 and p=0.743).Conclusion: Hypoxia altered expression of endothelial junction protein in placental capillaries, but no association with intraventricular hemorrhage was observed.
The combination of Acalypha indica–Centella asiatica extracts decreases the neuronal damage in hypoxia-induced hippocampal injury animal model Lisnawati, Lisnawati; Farida, Siti; Krisnamurti, Desak G.B.; Mudjihartini, Ninik; Purwaningsih, Erni H.; Sianipar, Imelda M.
Medical Journal of Indonesia Vol 27, No 3 (2018): September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (590.235 KB) | DOI: 10.13181/mji.v27i3.1697

Abstract

Background: Approximately 80–85% of strokes are ischemic and lead to alterations in neuronal cell morphology and cell death. There is a lack of studies on the effect of the combination of Acalypha indica L. (AI) and Centella asiatica L. (CA) in terms of its neurotherapy property. This study was conducted to investigate the neurotherapeutic effect of the combination of AI–CA extracts in improving rat’s hippocampal neuron injury post-hypoxia.Methods: A total of 36 Sprague-Dawley rats were categorized into six groups and placed in a hypoxia chamber for 7 consecutive days. Then, they were moved to normoxia cages and treated for 7 consecutive days as follows: control group without treatment as a negative control; treatment groups were administered citicoline 50 mg/kgBW as a positive control; three different dose combinations of AI150–CA150, AI200–CA150, and AI250–CA150 mg/kgBW, respectively. Histological analyses were performed to assess the improvement in nerve cell damage in the hippocampus.Results: Treatment with citicoline significantly decreased the damage of nerve cells (30.8%); the combination of the AI–CA extracts of AI150–CA150, AI200–CA150, and AI250–CA150 also significantly decreased the damage of nerve cells (36%, 36.4%, and 30.4%, respectively) compared to the control rats (15.4%).Conclusion: The combination of AI–CA extracts decreased the neuronal damage in the hypoxia-induced hippocampal injury animal model. The improvement effect of the combination of AI–CA extracts was not significantly different to citicoline.
STRES OKSDATIF OTAK TIKUS PADA INDUKSI HIPOKSIA SISTEMIK KRONIK Ninik Mudjihartini,* Sri Widia,* Fransiscus Suyatna,** Mohamad Sadikin*
NEURONA Vol. 34 No. 3 Juni 2017
Publisher : Neurona Majalah Kedokteran Neuro Sains

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

INTRODUCTION BRAIN IS A METABOLICALLY ACTIVE ORGAN WHICH CONSUMES 20 OF TOTAL OXYGEN AND GLUCOSE ENTERING THE HUMAN BODY AS A RESULT OF HIGH ENERGY METABOLISM LEVEL BRAIN BECOME MORE SENSITIVE TO OXIDATIVE STRESS
Peran reseptor nuklear pada implantasi dan perkembangan blastokista Xavierees, Endrico; Mudjihartini, Ninik
Jurnal Biomedika dan Kesehatan Vol 4 No 1 (2021)
Publisher : Fakultas Kedokteran Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/JBiomedKes.2021.v4.37-46

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Syarat penting untuk terjadinya kehamilan, yaitu blastokista pada fase tertentu siap melakukan implantasi dan proliferasi endometrium, sehingga menjadi reseptif terhadap embrio untuk melakukan implantasi. Pada tahap selanjutnya, masih terdapat proses molekular yang berkesinambungan sampai pada akhirnya terjadi hubungan langsung antara blastokista dan dinding endometrium dengan membentuk plasenta. Implantasi yang tidak efisien tentunya akan menyebabkan kegagalan implantasi, sehingga muncul masalah infertilitas. Sebanyak 30% kasus kegagalan kehamilan disebabkan oleh masalah kesehatan embrio. Pemahaman yang lebih mendalam mengenai sinyal-sinyal yang berperan pada kedua proses berkesinambungan tersebut diharapkan dapat memberikan metode terapi yang baru pada kasus infertilitas, sehingga meningkatkan jumlah kehamilan.
Brain-derived neurotrophic factor (BDNF) dan proses penuaan: sebuah tinjauan Mudjihartini, Ninik
Jurnal Biomedika dan Kesehatan Vol 4 No 3 (2021)
Publisher : Fakultas Kedokteran Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/JBiomedKes.2021.v4.120-129

Abstract

Brain-derived neurotrophic factor (BDNF) merupakan faktor neurotrofin yang berpengaruh dalam mendukung pembentukan, perkembangan neuron, dan mempertahankan keberadaan neuron. BDNF dapat ditemukan dan aktif pada hipokampus, korteks, dan otak depan. Penuaan merupakan proses multifaktorial yang ditentukan oleh faktor genetik dan faktor epigenetik. Penurunan kadar BDNF menurunkan kemampuan belajar dan mengingat, terjadi pada orang lanjut usia atau yang mengalami penyakit neurodegeneratif. Penelitian membuktikan bahwa kadar BDNF yang tinggi mampu meningkatkan plastisitas neuron. BDNF memiliki reseptor TrkB dan p75 yang menentukan suatu neuron dapat bertahan hidup atau sebaliknya terjadi apoptosis. BDNF-TrkB mendukung pembentukan short term memory dan perkembangan neuron khususnya dendrit. BDNF-p57 mengaktifkan NFkB yang berperan dalam maturasi sistem saraf pusat. Peran BDNF sangat dipengaruhi oleh reseptornya. Nutrisi, metabolisme, perilaku, dan stres memengaruhi ekspresi BDNF. Faktor lingkungan yang meningkatkan kadar BDNF dapat menunda penuaan dan meningkatkan kemampuan kognitif.
Serum Lactate Dehydrogenase Activity and Its Corellation with Carbohydrate Intake in Advanced Lung Cancer Patients claresta diella; Ninik Mudjihartini; Diana Sunardi; Dian Novita Chandra; Yulhasri Yulhasri; Achmad Mulawarman Jayusman
World Nutrition Journal Vol. 2 No. 2 (2019)
Publisher : Indonesian Nutrition Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25220/WNJ.V02.i2.0002

Abstract

Introduction : The aim of this study was to determine serum lactate dehydrogenase activity and its correlation with carbohydrate intake in advanced lung cancer patients at Dharmais National Cancer Hospital Jakarta. Cancer Cells are characterized by increase anaerobic glycolysis termed the Warburg effect with lactate as an end product, catalyzed by LDH enzyme. Activities of lactate in cancer influences on tumor growth initiation, survival, proliferation, angiogenesis and metastasis. Serum LDH activity can be used as a diagnostic, prognostic, and predictive marker for tumor sensitivity and resistancy to therapy.Methods :56 subjects of advanced lung cancer from Dharmais National Cancer Hospital Jakarta were included in this cross sectional study. Subjects were recruited by consecutive sampling. Food intake of total carbohydrate was obtained by utilizing 24 hours food recall method. The activity of  serum LDH (IU/L) was measured by using enzymatic spectrophotometry method on automated analyzer.Results :The mean of age subjects was 56,98 ± 10,36 years old and 55,4% were male. Carbohydrate intake based on 24 hours food recall was 57,64 ± 10,85%. The median of LDH activity was 541,5 (164–6539) IU/L, 60,7% was increase.Conclusion :This study showed medium negative significant correlation (p = 0,017, r = - 0,317) between total carbohydrate intake per day in grams with LDH serum activity. If carbohydrate source is reduced, the LDH enzyme will increase to keep the glycolysis process going. The results of this study indicate that an adequate carbohydrate needed in patients with cancer.
Correlation Between Docosahexaenoic Acid Intake and It’s Content in Breast Milk of Lactating Mothers in Jakarta Raphael Kosasih; Ninik Ninik Mudjihartini; Saptawati Bardosono
World Nutrition Journal Vol. 3 No. 1 (2019): Volume 03 Issue 1 Include Supplements Oral Presentation Abstracts of 14th Sympo
Publisher : Indonesian Nutrition Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25220/WNJ.V03.i1.0005

Abstract

Objective: Docosahexaenoic acid (DHA) is the predominant structural fatty acid in the brain and one of the most important nutrient for cognitive development in early life. During early life, there is very limited metabolic capability to convert omega-3 fatty acids to DHA. Therefore, newborn intake of DHA completely depends on preformed DHA in mother’s breast milk. This study aims to determine DHA intake among lactating mothers and their association with breast milk’s DHA.Method: This cross-sectional study was conducted in Grogol Petamburan and Cilincing Public Health Centre in Jakarta. Eighty healthy lactating mothers aged 20–35 years old in 1–6 months postpartum was taken using consecutive sampling method. Mother’s DHA intake was assessed with a semiquantitative food frequency questionnaire. Post-feed breast milk specimens were collected in the morning, transported with cool boxes, and stored in the laboratory at -70° C before the analyses. Breast milk DHA content was analysed using Gas Chromatography with Mass Spectrometry. Descriptive analyses and Spearman rho test was used with a 95% confidence level.Result: This study showed the median of subjects’ DHA intake was 158.5(13.9–719.7) mg/day, i.e. 67.5% of the subjects was below Food and Agriculture Organization (FAO) recommendation (200 mg/day). The median of breast milk DHA was 51.7(19–184.7) mg/day, only 42.5 % of the subjects had breast milk DHA to meet the minimal requirement of their infant based on FAO recommendation (0.1% of total energy requirement). There was a moderate correlation between subject DHA intake with breast milk’s DHA content (r = 0.478, p < 0.001).  Conclusion:  More than half of the subjects had DHA intake below FAO recommendation. Our finding showed a positive moderate correlation between DHA intake and breast milk DHA among lactating mothers.
Correlation between Serum Hugh Sensitivity C-Reactive Protein With Dietary Intake of Indonesian Lactating Mothers Karin Wiradarma; Diana Sunardi; Ninik Mudjihartini
World Nutrition Journal Vol. 3 No. 1 (2019): Volume 03 Issue 1 Include Supplements Oral Presentation Abstracts of 14th Sympo
Publisher : Indonesian Nutrition Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25220/WNJ.V03.i1.0008

Abstract

Background and Objectives:Chronic low-grade inflammation has emerged as important pathophysiology of non-communicable diseases, which can cause negative effects to mother and baby. Dietary intake has been known as important factor to affect inflammation, which can be measured by high-sensitivity C-reactive protein (hs-CRP). This study aimed to examine the correlation between serum hs-CRP with dietary intake in lactating mothers.Methods:A cross-sectional study was conducted by consecutively enrolling 71 lactating mothers, 3-6 months post-partum, age 20-35 years old, visiting Grogol Petamburan and Cilincing community health center between February and April 2019. Dietary assessment was conducted using semi-quantitative food frequency questionnaire. Anthropometric measurements included were pre-pregnancy weight, post-partum weight, and body height. Serum hs-CRP was measured by immunoturbidimetry method. Spearman correlation was used, with p<0.05 considered significant.Results:Correlation was found between serum hs-CRP and energy (r = 0.372, p = 0.001), carbohydrate (r = 0.295, p = 0.013), and vitamin B6 (r = -0.285, p = 0.016) intake. We also found that serum hs-CRP was correlated with pre-pregnancy (r = 0.296, p = 0.012) and post-partum BMI (r = 0.430, p<0.001).Conclusion:Energy, carbohydrate, and vitamin B6 intakes are positively correlated with serum hs-CRP level.
Vitamin E and Vitamin C Intake Among Lactating Mothers In Jakarta Sheira Taflah Putri Handana; Diyah Eka Andayani; Ninik Mudjihartini
World Nutrition Journal Vol. 3 No. 1 (2019): Volume 03 Issue 1 Include Supplements Oral Presentation Abstracts of 14th Sympo
Publisher : Indonesian Nutrition Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25220/WNJ.V03.i1.0009

Abstract

Background and ObjectivesVitamin E is a lipid soluble vitamin which obtain only through diet. Vitamin E has many functions including antioxidant and well known to prevent lipid peroxidation in membrane cell. Vitamin C helps to regenerate vitamin E back into non radical. Vitamin E and C intake among vulnerable group such as lactating mother still uncleared especially in Jakarta. The aim of this study to determine level of vitamin E and C intake among lactating mothers in JakartaMethods60 lactating mothers aged 20–40 years old in 1–6 months postpartum were recruited on Grogol Petamburan and Cilincing Public Health Centre in Jakarta. This cross sectional study held from March 2019 until April 2019. Vitamin E and C dietary intake were collected with semi quantitative food frequency questionnaire. Descriptive analysis was used in this study.ResultsThis study showed that median value  of vitamin E intake was 6.50 (1.20-43.10) gram/day with 91.7% subjects does not meet vitamin E recommended daily intake (RDA) recommendation (19 gram/day), otherwise 70% vitamin C intake was above RDA recommendation (100 gram/day) with median value is 120.05 (23.0-479.2) gram/day.ConclusionVitamin E intake among lactating mothers in Jakarta was not adequate and far below RDA recommendation but vitamin C intake among lactating mothers in Jakarta was adequate and met RDA recommendation.
Co-Authors Abd. Rasyid Syamsuri Achmad Mulawarman Jayusman Ahmad A. Jusuf Almira Devina Gunawan Andriani Andriani Ani Retno Prijanti Astrid Mariam Khairani Siregar claresta diella Dian Novita Chandra Diana Sunardi Diyah Eka Andayani Diyah Eka Andayani Ekawati, Maria Erni Purwaningsih Estiana Filzadiyanti Hadiyati Fudla Helena Fabiani Ika Superti Daruningrum Inge Permadhi Jusman, Sri Widia Azraki Karin Wiradarma Khusun, Helda Krisnamurti, Desak G.B. Livia Kurniati Saputra Mohamad Sadikin Mohamad Sadikin Mohamad Sadikin Mohamad Saekhu Nani Dharmasetiawani Novi Dewi Tanjung Pungguri Ayu Nega Sarsanti Purwaningsih, Erni H. Rahmawaty Ridwan, Rahmawaty Raphael Kosasih Ray Sugianto, Ray Reni Paramita Rita Lahirin, Rita Saptawati Bardosono Saptawati Bardosono Sheira Taflah Putri Handana Sheira Taflah Putri Handana Sianipar, Imelda M. Siti Farida Sri W.A. Jusman Sri Widia A Jusman Sri Widia A. Jusman Wiji Lestari Xavierees, Endrico Yulhasri Yulhasri