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Jumlah Koloni Bakteri Selulolitik pada Sekum Ayam Kampung (Gallus domesticus) raudhatul jannah jannah; Safika Safika; Muhammad Jalaluddin
JURNAL ILMIAH MAHASISWA VETERINER Vol 1, No 3 (2017): MEI - JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (504.5 KB) | DOI: 10.21157/jim vet..v1i3.4023

Abstract

Penelitian ini bertujuan menghitung jumlah koloni bakteri selulolitik pada sekum ayam kampung. Sampel yang digunakan yaitu isi sekum ayam kampung yang diambil di Tempat Pemotongan Unggas (TPU) Lambaro Aceh Besar sebanyak 5 ekor ayam kampung. Sampel dilakukan pengenceran 10-2 sampai 10-5, kemudian diinokulasi ke dalam media BHM-CMC agar dengan metode tuang (pour plate). Selanjutnya, diinkubasi dengan suhu 37˚C selama 96 jam. Koloni bakteri selulolitik yang tumbuh kemudian dilakukan pengamatan morfologi koloni (warna, posisi, pinggiran, dan diameter koloni), diameter zona bening, penghitungan jumlah total bakteri, dan pewarnaan Gram. Hasil penelitian menunjukkan bahwa koloni bakteri berwarna putih, berada di dalam dan di permukaan agar, memiliki pinggiran rata, diameter koloni 2-3 mm dan diameter zona bening 18 mm dan 29 mm. Bakteri berbentuk basil bereaksi negatif terhadap pewarnaan Gram, sedangkan bakteri berbentuk kokus bereaksi positif dan negatif terhadap pewarnaan Gram. Jumlah total bakteri pada isi sekum ayam kampung yaitu 5.3 x 105 cfu/g.Kata kunci: Ayam kampung, bakteri selulolitik, sekum, Carboxy Methyl Cellulase.                The aim of this study was to count the total count of cellulolytic bacteria colony in caecum of native chicken. The sample used was caecum contents of native chicken which taken from the poultry slaughterplace at Lambaro Aceh Besar. Sample were diluted from 10-2 to 10-5, then inoculated to agar BHM-CMC media with pour plate method. Then, incubated with temperature 37oC about 96 hours. A colony of cellulolytic bacteria which were grown then were observed by using colony bacteria criteria (colour, position, edge, and diameter of the colony), diameter of the bacterial zone, counting a total of bacteria which was grown, and Gram stain. The results showed that there is white bacterial colony, located inside and surface agar, has flat periphery, diameters of colony 2-3 mm and diameters of bacterial zone 18 and 29 mm. Bacill-shaped bacteria react negative to Gram staining, whereas coccus-shaped bacteria react positive and negative to Gram staining. Number of total bacteria in the native chicken’s caecum was 5.3 x 105 cfu/g Keywords: Native chicken, cellulolytic bacteria, caecum, Carboxy Methyl Cellulose.
MULTIDRUG RESISTANCE OF Klebsiella pneumoniae IN CATS IN BOGOR, INDONESIA Juliadi Ramadhan; Safika Safika; Ni Luh Putu Ika Mayasari
Jurnal Kedokteran Hewan Vol 15, No 2 (2021): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i2.17882

Abstract

 This study aims to measure the level of antibiotic resistance to Klebsiella pneumoniae isolated from clinical cats in Bogor. Samples were isolated and identified macroscopically, microscopically, and biochemically. Positive isolates were tested for antibiotic sensitivity using the Kirby-Bauer disk diffusion method. The results showed that Klebsiella pneumoniae isolated from sputum and laryngeal swabs of clinic cats in Bogor had experienced Multidrug Resistance (MDR). The highest level of resistance to Klebsiella pneumoniae occurred in the β-lactam group (amphicillin 76%) followed by the tetracycline group (oxytetracycline 72% and tetracycline 68%), then the quinolone group (enrofloxacin 52%), and finally the aminoglycoside group (gentamicin 44%). The results of this study are expected to be taken into consideration in the use of antibiotics for the treatment of cases related to the Klebsiella pneumoniae bacteria.
C/N ratio and pH during composting of cattle manure Safika, S; Madayanti, F; Aditiawati, P; Akhmaloka, A
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Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

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Abstract

Abstract
Uji Resistensi Terhadap Beberapa Antibiotika pada Escherichia coli yang Diisolasi dari Kucing di Klinik Hewan Kota Bogor Yamin Yaddi; Safika Safika; Fachriyan Hasmi Pasaribu
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 7, No 3 (2020): JITRO, September
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (109.938 KB) | DOI: 10.33772/jitro.v7i3.13442

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ABSTRAKPermasalahan resistensi Antibiotika pada hewan kesayangan menjadi kendala kesehatan hewan di seluruh dunia. World Health Organisation (WHO) menyebutkan bahwa pada masa mendatang resistensi antibiotika akan menjadi tantangan yang terbesar dalam dunia kesehatan. Penelitian ini bertujuan untuk mengukur tingkat resistensi antibiotika terhadap Escherichia coli yang diisolasi dari kucing pada klinik hewan di Kota Bogor. Hasil penelitian menunjukan bahwa resistensi Escherichia coli tertinggi terjadi pada golongan β-laktam (ampisilin 66% dan amoksisilin 60%) yang diikuti oleh golongan tetrasiklin (oksitetrasiklin 54% dan dosisiklin 24%), serta golongan kuinolon (enrofloksasin 38% dan ciprofloksasin 28%). Hasil dari penelitian ini diharapkan dapat menjadi pertimbangan medis bagi praktisi hewan kesayangan dalam penggunaan antibiotika.Kata Kunci: Escherichia coli, klinik hewan, kucing, resistensi antibiotikaABSTRACTThe problem of antibiotic resistance in pets is obstacles to animal health throughout the world. World Health Organization (WHO) states that in the future, antibiotic resistance will become the biggest challenge in the health concern. This study aims to measure the level of Escherichia coli resistance to antibiotics which is isolated from cats on veterinary clinics in Bogor City. The results showed that the highest resistance of Escherichia coli occurred in the β-lactam group (ampicillin 66% and amoxicillin 60%) followed by tetracycline (oxytetracycline 54% and doxycycline 24%), and quinolone group (enrofloxacin 38% and ciprofloxacin 28%). This study is expected to become medical considerations for pet practitioners in the use of antibiotics.Keywords: animal clinic, antibiotic resistance, cats, Escherichia coli
19. The Effect of AKBIS Probiotic Adding that Fermented in Fodder toward the Total of Aspergillus niger of Broiler’s Gastrointestinal Tract Cut Lathifa Nurrady; Nurliana Nurliana; Safika Safika; T. Reza Ferasyi; Ismail Ismail; Muttaqien Muttaqien
Jurnal Medika Veterinaria Vol 12, No 2 (2018): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (313.507 KB) | DOI: 10.21157/j.med.vet..v12i2.4051

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This study aimed to determine the total of A. niger found in the gastrointestinal tract of broilers after the addition of soybean dregs and fermented palm kernel meal by A.niger as probiotics (AKBIS Prob) in the fodder. This study used a complete randomized factorial design, which consists of two factors, namely factor AKBIS concentration and duration of AKBIS Prob. The treatment was divided into the group as a control P0, P1, P2 and P3 which consisted of five broilers, respectively given feed supplemented with AKBIS Prob 2, 4 and 6%. The provision of AKBIS Prob has done for 21 days and 35 days. On the 22nd and 36th day the contents of the caecum were taken. The total of A. niger was obtained using Total Plate Count (TPC) method. The data analysis used a factorial analysis of variance. The results showed that the concentration of 2, 4 and 6% did not affect (P 0.05) the total of A. niger in the caecum of broilers, while the provision of AKBIS prob in fodder for 21 and 35 days had a significant effect (P 0.05) to the total A. niger. It can be concluded that the provision of the concentration of AKBIS Prob up to 6% in the fodder decreases the total of A. Niger. The provision of AKBIS Prob for 35 days can increase the total of A. niger in the digestive tract of broilers.
JUMLAH KOLONI BAKTERI SELULOLITIK PADA ILIUM AYAM KAMPUNG (Gallus domesticus) nurjannaini nurjannaini; safika safika; M. Jalaluddin M. Jalaluddin
JURNAL ILMIAH MAHASISWA VETERINER Vol 1, No 3 (2017): MEI - JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (536.039 KB) | DOI: 10.21157/jim vet..v1i3.4042

Abstract

Penelitian ini bertujuan menghitung jumlah koloni bakteri selulolitik pada ilium ayam kampung. Sampel yang digunakan yaitu isi ilium 5 ekor ayam kampung yang diambil di Tempat Pemotongan Unggas (TPU) Lambaro Aceh Besar. Sampel yang diperoleh dilakukan pengenceran 10-2 sampai 10-5, kemudian diinokulasi ke dalam media BHM-CMC agar dengan metode tuang (pour plate). Selanjutnya, diinkubasi dengan suhu 37˚C selama 96 jam. Koloni bakteri selulolitik yang tumbuh kemudian dilakukan pengamatan morfologi koloni (warna, posisi, pinggiran, dan diameter koloni), diameter zona bening, penghitungan jumlah total bakteri dan pewarnaan Gram. Hasil penelitian menunjukkan bahwa terdapat morfologi koloni berwarna putih bening, posisi di dalam dan di permukaan agar, pinggiran bergerigi, diameter koloni 2-4 mm dan zona bening 19 mm sampai 26 mm. Bakteri selulolitik berbentuk basil Gram negatif dan kokus Gram positif. Rata-rata jumlah total bakteri pada isi ilium ayam kampung yaitu 3,3 x 105 cfu/g. The aim of this study was to count the total sum of cellulolytic bacteria colony in the illium of native chicken. The sample used was illium contents of native chicken whic taken from the poultry slaughterhouse at Lambaro Aceh Besar. Sample were diluted from 10-2 to 10-5, then inoculated to agar BHM-CMC media with pour plate method. After that, incubated with temperature 37oC about 96 hours. A colony of cellulolytic bacteria which were grown then were observed by using colony bacteria criteria (colour, position, edge, and diameter of the colony), diameter of the bacterial zone, counting a total of bacteria which was grown, and Gram stain. The results showed that there is white translucent colony morphology, position inside and surface agar, cogged periphery, diameters of colony 2-4 mm and bacterial zone 19 to 26 mm. Cellulolytic bacteria Gram-negatif bacilli, Gram-negative cocci and Gram-positive cocci. The average number bacteria of total bacteria in the native chicken’s illium was 3.3 x 105 cfu/g.
Deteksi Gen Penyandi Resistansi blaTEM, blaSHV, dan blaCTXM pada Pseudomonas aeruginosa Ayam Petelur di Kabupaten Cianjur, Jawa Barat Safika Safika; Fauzan Arisandi; Fachriyan Hasmi Pasaribu; Yamin Yaddi
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 1 (2022): JITRO, Januari 2022
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (414.859 KB) | DOI: 10.33772/jitro.v9i1.20448

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ABSTRAKPseudomonas aeruginosa merupakan bakteri oportunistik patogen yang mampu meninfeksi bagi hewan dan manusia. Resistansi terhadap banyak antibiotik memberikan tantangan yang cukup besar dalam pengobatan infeksi Pseudomonas aeruginosa. Penelitian ini bertujuan mendeteksi adanya resistansi antibiotik dan gen penyandi resistansi pada isolat bakteri Pseudomonas aeruginosa yang diisolasi dari peternakan ayam petelur di Kabupaten Cianjur, Jawa Barat. Sampel diisolasi dan identifikasi sebanyak enam puluh enam melalui usap kloaka. Sampel yang dikoleksi dilakukan kultur pada media selektif (MacConkey agar), dilanjutkan dengan uji mikroskopik, uji biokimia, dan dikonfirmasi dengan secara molekuler dengan polymerase chain reaction (PCR). Sampel yang positif diuji kepekaan terhadap antibiotik menggunakan metode Kirby-Bauer disk diffusion dan mendeteksi gen penyandi resistansi. Hasil penelitian 8 sampel bakteri Pseudomonas aeruginosa dilakukan uji kepekaan antibiotik menunjukkan tingkat resistansi terhadap golongan antibiotik beta laktam (ampisilin 75%) dan aminoglikosida (gentamisin 0%). Dekteksi gen penyandi resistansi secara berturut-turut menunjukkan gen blaTEM (100%), blaCTXM (100%) terdeteksi, sedangkan gen blaSHV tidak terdeteksi pada isolat yang diuji. Perlunya dilakukan penelitian lanjutan untuk mendeteksi sampel dari lingkungan, tempat air minum, pakan maupun karyawan di peternakan tersebut. Sehingga memeberikan informasi dan kajian ilmiah untuk pengaturan regulasi penggunaan antibiotik di peternakan.Kata Kunci: antibiotik, ayam petelur, gen resisten, Pseudomonas aeruginosaDetection of blaTEM, blaSHV, and blaCTXM Resistance Coding Genes in Pseudomonas aeruginosa Layer Chickens in Cianjur Regency, West JavaABSTRACTPseudomonas aeruginosa is a pathogenic opportunistic bacteria capable of infecting animals and humans. Resistance to many antibiotics presents considerable challenges in the treatment of Pseudomonas aeruginosa infections. This study aims to detect the presence of antibiotic resistance and genes encoding resistance in isolates of Pseudomonas aeruginosa isolated from laying hens in Cianjur Regency, West Java. Sixty-six samples were isolated and identified through cloacal swab. The collected samples were cultured on selective media (MacConkey agar), followed by microscopic tests, biochemical tests, and confirmed molecularly by polymerase chain reaction (PCR). Positive samples were tested for susceptibility to antibiotics using the Kirby-Bauer disk diffusion method and detected genes encoding resistance by PCR. The results of the study of 8 samples of Pseudomonas aeruginosa bacteria were tested for antibiotic sensitivity showing the level of resistance to beta-lactam antibiotics (ampicillin 75%) and aminoglycosides (gentamicin 0%). The detection of resistance coding genes, respectively, showed that blaTEM (100%), blaCTXM (100%) genes were detected, while the blaSHV gene was not detected in the tested isolates. Further research is needed to detect samples from the environment, drinking water, feed and employees on the farm. So that it provides information and scientific studies to regulate the regulation of the use of antibiotics in livestock.Keywords: antibiotic, laying hens, Pseudomonas aeruginosa, resistant genes
KOSAKONIA SP. PROTEOLYTIC BACTERIA ISOLATED FROM RUMEN AND RETICULUM OF ACEH CATTLE Safika Safika; Wenny Novita Sari; Gressha Vionalle Ademi; Ulfi Widi Arsih; Darmawi Darmawi
Jurnal Kedokteran Hewan Vol 14, No 2 (2020): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i2.16426

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The aim of this study was to identify proteolytic bacteria from the ruminal and reticulum fluids of aceh cattle based on the 16S rRNA gene. Samples used were ruminal and reticulum fluids of aceh cattle slaughtered in Abattoir of Aceh Besar. Samples were diluted and cultured into Skim Milk Agar medium at 39 C for 48 hours. The morphology of bacterial colonies growth in the medium was observed. Colonies resulted in the largest clear zone were isolated and used for Deoxyribonucleic Acid (DNA) isolation, 16S rRNA gene amplification and sequencing. Theresults showed that morphology of dominant colonies was yellowish white color, round shape, position on the agar surface. The results ofphylogenetic analysis of RS1 and ReS2 isolates isolated from rumen and reticulum fluids of aceh cattle respectively had a close familialrelationship and belonged to the bacterial group of Kosakonia. Sequence homology showed isolate RS1 and ReS2 are probably either newEnterobacteriaceae species or unconfirmed species. Halo zone produced by ruminal bacteria had a wider diameter (25 mm vs 20 mm) than thatcaused by reticulum bacteria). Based on the results, RS1 (bacterium in the rumen) and ReS2 (bacterium in the reticulum) belong to similar type, namely Kosakonia sp. with a proteolytic activity. Presumably, these bacteria originate from the rumen that enters the reticulum with degraded feed.
Correlation of Aspergillus flavus with the Concentration of Aflatoxin B1 in Ikan Kayu Safika Safika
Jurnal Kedokteran Hewan Vol 2, No 2 (2008): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v2i2.9784

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The objective of this research was to know the contamination of Aspergillus flavus in ikan kayu and the number of aflatoxin B1 (AFB1) produced. Also the corelation the number of A. flavus with AFB1. Samples were taken from traditional markets in Banda Aceh and Lhokseumawe. The sample were used to isolation and identify A. flavus also detect the present of AFB1 by ELISA (Enzym Linked ImmunosorbantAssay). The result shows that all samples show the present of A. flavus with the concentration 1 x 102-61 x 102 Cfu/g and the concentration of AFB1 between 0.9-38.7 ppb. In conclusion the present A. flavus in ikan kayu has a corelation with the production of AFB1.
PENENTUAN AFLATOKSIN B1 PADA MAKANAN OLAHAN KACANG TANAH DENGAN MENGGUNAKAN ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) Siti Aisyah; Safika S; Faisal Jamin
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i1.2785

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Penelitian ini bertujuan mengetahui kontaminasi Aspergillus flavus dan konsentrasi aflatoksin B1 pada jenis makanan berbahan baku kacang tanah yang dipasarkan di Banda Aceh. Pengambilan sampel dilakukan di pasar tradisional dan swalayan di Banda Aceh. Sampel diisolasi dan diidentifikasi A. flavus serta dideteksi AFB1 dengan menggunakan metode enzym-linked immunosorbent assay (ELISA). Berdasarkan hasil penelitian yang dilakukan dari 15 sampel makanan berbahan baku kacang tanah, 73,33% (11 sampel) terkontaminasi aflatoksin B 1 dengan konsentrasi yang bervariasi. Walaupun terkontaminasi aflatoksin B1, namun sebanyak 86,67% sampel makanan berbahan baku kacang tanah masih aman dikomsumsi sesuai dengan persyaratan yang ditetapkan pemerintah yaitu maksimal 20 ppb.