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Phenotypic and Genotypic Study of Antibiotics Resistance Profile in Escherichia coli Isolated from Broilers in Cianjur, Indonesia Aprilia Hardiati; Safika; I Wayan Teguh Wibawan; Fachriyan Hasmi Pasaribu
Acta VETERINARIA Indonesiana Vol. 9 No. 2 (2021): Juli 2021
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.9.2.97-104

Abstract

This study aimed to investigate the phenotypic and genotypic of antibiotics resistance profile in Escherichia coli. The 30 samples come from cloacal swab of broilers in Cianjur, Indonesia. Isolation and identification of E. coli was performed by culture in McConkey agar, eosin methylene blue agar, Gram staining and five essential biochemical tests (IMViC). In this study, 10 isolates (33.3%) were confirmed E. coli positive. Phenotypic profile was performed by screening all isolates with 8 antibiotics of 6 antibiotic groups. The screening was carried by Kirby-Bauer disk diffusion method based on the standard of CLSI. For genotypic profile, each resistant isolate was detected antibiotic resistance-encoding gene. The result showed all isolates (100%) resistant against tetracyclin, oxytetracycline and erythromycin. Nine isolates (90%) detected nalidixic acid and enrofloxacin-resistant. The ciprofloxacin and gentamicin-resistant isolates were 70% and 40%, respectively. There was no resistant isolate for chloramphenicol. Multi drug-resistant was detected on 90% isolates. Only gyrA (100%) and tetA (80%) genes were detected. This study showed high rate of occurrence of antibiotic resistance in E. coli. Not all resistant isolates were detected in the antibiotic resistance-encoding gene in this study. Future research to detect resistance genes should use more varied target genes.
MP-5 Antibiotic Resistance of Klebsiella Species Isolated from Broiler Chickens in Sukabumi and Bogor Areas Zumala Nilasari; . Safika; Fachriyan Hasmi Pasaribu
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (724.185 KB)

Abstract

Animal protein needs in Indonesia increase every year. One source of animal protein that is affordable for all levels of society is poultry. Poultry mostly farmed in several parts of Indonesia. The region that has the largest poultry population in Indonesia is the province of West Java. The regions in West Java that contribute the highest poultry populations are Sukabumi and Bogor. The largest number of poultry populations is broiler chicken population, which is 108,304,978 tails.Antibiotics often used in livestock and poultry as a prevention of disease transmission and as a growth promoter. Routine use of antibiotics in the livestock industry, especially chickens, has a negative impact. One of them is the emergence of antibiotic resistance. One of the bacteria that has experienced antibiotic resistance is the Klebsiella species.Klebsiella bacteria are Gram-negative bacteria are normal flora in the oral cavity, skin and intestines, but can become pathogens in animals and humans under certain conditions. This bacterium is one of the causes of bacterial diseases that attack large livestock and poultry.  Many reports say that Klebsiella species have experienced resistance to antibiotics. Klebsiella had experienced resistance to several antibiotics, namely ampicillin as much as 66.7%, Nalidixic Acid 61.8%, tetracycline 59.8% and trimethropin 50%. Klebsiella bacteria that are resistant to antibiotics are very dangerous for animal and human health. Diseases caused by Klebsiella bacteria that are resistant to antibiotics will be difficult to cure. Akova (2016) states that Gram-negative and Gram-positive bacteria that experience resistance will produce infections that are difficult to treat or cannot even be treated using antimicrobials.
MP-14 Antibiotics Resistance Patterns of Escherichia coli Isolated from Poultry in West Java Aprilia Hardiati; . Safika; Fachriyan Hasmi Pasaribu
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (521.213 KB)

Abstract

Most of poultry industries use antibiotics for health management program. They use them as bacterial infection treatment and disease prevention, known as antibiotic growth promotor (AGP) [1]. Now, worldwide concern is about antibiotics resistance. Monitoring programs are done by countries in the world to protect human and animal health [2]. The monitoring programs usually use indicator bacteria such as Escherichia coli [3].Many researchers studied about antibiotics resistance in Indonesia. Escherichia coli isolated from poultry has been resistance to doxycycline (25%) and gentamycin (12.5%) [4]. Seven E. coli isolated from fecal samples shown that resistance to methicillin (85.7%), penicillin G (71.4%) and 42,9% were resistance both doxycycline hydrochloride and streptomycin [5]. Start from Januari 1st 2018 Indonesian Misintry of Agricuture banned antibiotics as AGP, based on Permentan No. 14/2017. They tried to prevent the spread of antibiotics resistance. So, it is necessary to determine antibiotics resistance patterns, especially in E. coli as indicator bacteria.
Deteksi Gen Penyandi Resistansi blaTEM, blaSHV, dan blaCTXM pada Pseudomonas aeruginosa Ayam Petelur di Kabupaten Cianjur, Jawa Barat Safika Safika; Fauzan Arisandi; Fachriyan Hasmi Pasaribu; Yamin Yaddi
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 1 (2022): JITRO, Januari 2022
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (414.859 KB) | DOI: 10.33772/jitro.v9i1.20448

Abstract

ABSTRAKPseudomonas aeruginosa merupakan bakteri oportunistik patogen yang mampu meninfeksi bagi hewan dan manusia. Resistansi terhadap banyak antibiotik memberikan tantangan yang cukup besar dalam pengobatan infeksi Pseudomonas aeruginosa. Penelitian ini bertujuan mendeteksi adanya resistansi antibiotik dan gen penyandi resistansi pada isolat bakteri Pseudomonas aeruginosa yang diisolasi dari peternakan ayam petelur di Kabupaten Cianjur, Jawa Barat. Sampel diisolasi dan identifikasi sebanyak enam puluh enam melalui usap kloaka. Sampel yang dikoleksi dilakukan kultur pada media selektif (MacConkey agar), dilanjutkan dengan uji mikroskopik, uji biokimia, dan dikonfirmasi dengan secara molekuler dengan polymerase chain reaction (PCR). Sampel yang positif diuji kepekaan terhadap antibiotik menggunakan metode Kirby-Bauer disk diffusion dan mendeteksi gen penyandi resistansi. Hasil penelitian 8 sampel bakteri Pseudomonas aeruginosa dilakukan uji kepekaan antibiotik menunjukkan tingkat resistansi terhadap golongan antibiotik beta laktam (ampisilin 75%) dan aminoglikosida (gentamisin 0%). Dekteksi gen penyandi resistansi secara berturut-turut menunjukkan gen blaTEM (100%), blaCTXM (100%) terdeteksi, sedangkan gen blaSHV tidak terdeteksi pada isolat yang diuji. Perlunya dilakukan penelitian lanjutan untuk mendeteksi sampel dari lingkungan, tempat air minum, pakan maupun karyawan di peternakan tersebut. Sehingga memeberikan informasi dan kajian ilmiah untuk pengaturan regulasi penggunaan antibiotik di peternakan.Kata Kunci: antibiotik, ayam petelur, gen resisten, Pseudomonas aeruginosaDetection of blaTEM, blaSHV, and blaCTXM Resistance Coding Genes in Pseudomonas aeruginosa Layer Chickens in Cianjur Regency, West JavaABSTRACTPseudomonas aeruginosa is a pathogenic opportunistic bacteria capable of infecting animals and humans. Resistance to many antibiotics presents considerable challenges in the treatment of Pseudomonas aeruginosa infections. This study aims to detect the presence of antibiotic resistance and genes encoding resistance in isolates of Pseudomonas aeruginosa isolated from laying hens in Cianjur Regency, West Java. Sixty-six samples were isolated and identified through cloacal swab. The collected samples were cultured on selective media (MacConkey agar), followed by microscopic tests, biochemical tests, and confirmed molecularly by polymerase chain reaction (PCR). Positive samples were tested for susceptibility to antibiotics using the Kirby-Bauer disk diffusion method and detected genes encoding resistance by PCR. The results of the study of 8 samples of Pseudomonas aeruginosa bacteria were tested for antibiotic sensitivity showing the level of resistance to beta-lactam antibiotics (ampicillin 75%) and aminoglycosides (gentamicin 0%). The detection of resistance coding genes, respectively, showed that blaTEM (100%), blaCTXM (100%) genes were detected, while the blaSHV gene was not detected in the tested isolates. Further research is needed to detect samples from the environment, drinking water, feed and employees on the farm. So that it provides information and scientific studies to regulate the regulation of the use of antibiotics in livestock.Keywords: antibiotic, laying hens, Pseudomonas aeruginosa, resistant genes
Uji Resistensi Terhadap Beberapa Antibiotika pada Escherichia coli yang Diisolasi dari Kucing di Klinik Hewan Kota Bogor Yamin Yaddi; Safika Safika; Fachriyan Hasmi Pasaribu
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 7, No 3 (2020): JITRO, September
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (109.938 KB) | DOI: 10.33772/jitro.v7i3.13442

Abstract

ABSTRAKPermasalahan resistensi Antibiotika pada hewan kesayangan menjadi kendala kesehatan hewan di seluruh dunia. World Health Organisation (WHO) menyebutkan bahwa pada masa mendatang resistensi antibiotika akan menjadi tantangan yang terbesar dalam dunia kesehatan. Penelitian ini bertujuan untuk mengukur tingkat resistensi antibiotika terhadap Escherichia coli yang diisolasi dari kucing pada klinik hewan di Kota Bogor. Hasil penelitian menunjukan bahwa resistensi Escherichia coli tertinggi terjadi pada golongan β-laktam (ampisilin 66% dan amoksisilin 60%) yang diikuti oleh golongan tetrasiklin (oksitetrasiklin 54% dan dosisiklin 24%), serta golongan kuinolon (enrofloksasin 38% dan ciprofloksasin 28%). Hasil dari penelitian ini diharapkan dapat menjadi pertimbangan medis bagi praktisi hewan kesayangan dalam penggunaan antibiotika.Kata Kunci: Escherichia coli, klinik hewan, kucing, resistensi antibiotikaABSTRACTThe problem of antibiotic resistance in pets is obstacles to animal health throughout the world. World Health Organization (WHO) states that in the future, antibiotic resistance will become the biggest challenge in the health concern. This study aims to measure the level of Escherichia coli resistance to antibiotics which is isolated from cats on veterinary clinics in Bogor City. The results showed that the highest resistance of Escherichia coli occurred in the β-lactam group (ampicillin 66% and amoxicillin 60%) followed by tetracycline (oxytetracycline 54% and doxycycline 24%), and quinolone group (enrofloxacin 38% and ciprofloxacin 28%). This study is expected to become medical considerations for pet practitioners in the use of antibiotics.Keywords: animal clinic, antibiotic resistance, cats, Escherichia coli
2. Antibacterial Test of Red Dragon Fruit Extract Peel (Hylocereus polyrhizus) Against Bacteria Salmonella pullorum Ichsan Maulana; Abdul Harris; Fakhrurrazi Fakhrurrazi; Maryulia Dewi; Safika Safika; Erina Erina; Muhammad Jalaluddin
Jurnal Medika Veterinaria Vol 12, No 1 (2018): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (369.06 KB) | DOI: 10.21157/j.med.vet..v12i1.4241

Abstract

The purpose of this study is to determine the effect of the red dragon fruit peels (Hylocereus polyrhizus) extract on the growth of Salmonella pullorum. This research was conducted at the Laboratory of Microbiology, Veterinary Faculty, University of Syiah Kuala, Banda Aceh. Red dragon fruit peels that used in this study is obtained from  ripe fruit and Salmonella pullorum cultures is  obtained from  Microbiology Laboratory of Veterinary Faculty Unsyiah. Salmonella pullorum culture added evenly on the surface of Mueller Hinton Agar, the number of the bacteria have been standarized using Mc Farland'1 method. Discs soaked in the red dragon fruit peels extracts at different concentrations (20 mg/ml, 40 mg/mL, and 60 mg/ml). Ampicillin is used as positive controls and DMSO (dimethyl sulfoxide) was placed on the surface of Mueller Hinton Agar. The parameters in this study is the inhibition zone diameter. Data were analyzed descriptively. The results showed that the red dragon fruit peel extract is able to inhibit the growth of Salmonella  pullorum and the average of  inhibition diameter zone at a concentration 60 mg / ml is 9.6 mm, 40 mg / ml is 9.4 mm and 20 mg / ml is 9.3 mm
9. Total colony of cellulolitic bacteria in the rumen of aceh cattle Safika Safika; Siti Wardianah Matondang; Darmawi Darmawi; Mahdi Abral; Erina Erina; M Jalaluddin
Jurnal Medika Veterinaria Vol 11, No 1 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (454.784 KB) | DOI: 10.21157/j.med.vet..v11i1.3411

Abstract

The aim of this study was to count the total sum of cellulolitic bacteria colony in  aceh cattle rumen. The sample used was aceh cattle rumen liquid which taken from salughtered house in Banda Aceh. Aceh cattle rumen liquid were diluted from 10-2 to 10-5, then inoculated to agar BHM-CMC media with pour plate method. Petri dish was entered to candle jar to be incubated in an incubator with temperature 37oC about 96 hours. Colony of cellulolitic bacteria which were grown then were observed by using colony bacteria criteria (colour, position, edge, and diameter of colony), counting total of bacteria which was grown, and Gram staining. The results showed that there is a white translucent colony morphology, growth inside agar, smooth periphery, Gram-positive bacil. The average number bacteria of total bacteria in the cattle rumen liquid was 4,81 x 105 cfu/ml
DETEKSI AFLATOKSIN B1 PADA JENIS MAKANAN OLAHAN JAGUNG MENGGUNAKAN ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) Safika -; Faisal Jamin; Siti Aisyah
Jurnal Medika Veterinaria Vol 9, No 1 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (190.744 KB) | DOI: 10.21157/j.med.vet..v9i1.2988

Abstract

Penelitian ini bertujuan mengetahui konsentrasi AFB1 pada jenis makanan olahan asal jagung yang dipasarkan di Banda Aceh. Pengambilan sampel dilakukan di pasar dan swalayan di Banda Aceh. Deteksi AFB1 pada sampel menggunakan metode enzyme-linked immunosorbent assay (ELISA). Berdasarkan penelitian, dapat disimpulkan bahwa dari 6 sampel makanan berbahan baku jagung, 5 sampel (83,33% ) terkontaminasi AFB1. Walaupun terkontaminasi AFB1 namun semua sampel masih aman dikonsumsi sesuai dengan persyaratan yang ditetapkan pemerintah yaitu maksimal 20 ppb.
11. The Isolation And Identification Of Bacteria Salmonella Sp On Quail Egg Shell In Traditional Markets Ulee Kareng Banda Aceh Erina Erina; Azmansyah Azmansyah; Darniati Darniati; Fakhrurrazi Fakhrurrazi; Safika Safika; Tongku N Siregar
Jurnal Medika Veterinaria Vol 13, No 1 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (798.344 KB) | DOI: 10.21157/j.med.vet..v13i1.4226

Abstract

This research aim was to determine the level of bacterial contamination of Salmonella sp on quail egg. The samples used in this research was 24 shell of quail eggs obtained from eight stores in traditional markets in Ulee Kareung Banda Aceh. The shell was crushed with a mortar and then cultured on SCB media (selenite cysteine broth). Isolation and identification was conducted by using SSA media culture, Gram staining, and biochemical tests. The results of this research showed that the shell of quail eggs contained Salmonella sp. Therefore it can be concluded that the quail eggs sold in Ulee Kareung traditional market were contaminated by Salmonella sp.
19. The Effect of AKBIS Probiotic Adding that Fermented in Fodder toward the Total of Aspergillus niger of Broiler’s Gastrointestinal Tract Cut Lathifa Nurrady; Nurliana Nurliana; Safika Safika; T. Reza Ferasyi; Ismail Ismail; Muttaqien Muttaqien
Jurnal Medika Veterinaria Vol 12, No 2 (2018): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (313.507 KB) | DOI: 10.21157/j.med.vet..v12i2.4051

Abstract

This study aimed to determine the total of A. niger found in the gastrointestinal tract of broilers after the addition of soybean dregs and fermented palm kernel meal by A.niger as probiotics (AKBIS Prob) in the fodder. This study used a complete randomized factorial design, which consists of two factors, namely factor AKBIS concentration and duration of AKBIS Prob. The treatment was divided into the group as a control P0, P1, P2 and P3 which consisted of five broilers, respectively given feed supplemented with AKBIS Prob 2, 4 and 6%. The provision of AKBIS Prob has done for 21 days and 35 days. On the 22nd and 36th day the contents of the caecum were taken. The total of A. niger was obtained using Total Plate Count (TPC) method. The data analysis used a factorial analysis of variance. The results showed that the concentration of 2, 4 and 6% did not affect (P 0.05) the total of A. niger in the caecum of broilers, while the provision of AKBIS prob in fodder for 21 and 35 days had a significant effect (P 0.05) to the total A. niger. It can be concluded that the provision of the concentration of AKBIS Prob up to 6% in the fodder decreases the total of A. Niger. The provision of AKBIS Prob for 35 days can increase the total of A. niger in the digestive tract of broilers.