Garuda - Garba Rujukan Digital

p-Index From 2019 - 2024

3.207

P-Index

This Author published in this journals

All Journal ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Veteriner BIOTIK: Jurnal Ilmiah Biologi Teknologi dan Kependidikan Jurnal Ilmu dan Teknologi Peternakan Tropis Jurnal Medika Veterinaria Jurnal Ilmiah Mahasiswa Veteriner Tropical Animal Science Journal ARSHI Veterinary Letters Jurnal Natural Jurnal Kedokteran Hewan The International Journal of Tropical Veterinary and Biomedical Research

Safika S, Safika

Institut Pertanian Bogor

Author-ID : 520715

Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Energy Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Physics Public Health Veterinary

Published : 34 Documents Claim Missing Document

Claim Missing Document

Articles

1 2 3 4

12. Isolate Of Bacteriua Cellulase Enzyme Production In The Rumen Of Aceh Cattle Base On Analysis Homology 16S rRNA Indah Melzana; Safika Safika; Darmawi Darmawi; Erina Erina; Fakhrurrazi Fakhrurrazi; Al Azhar
Jurnal Medika Veterinaria Vol 14, No 2 (2020): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v14i2.3415

The rumen of cattle is well known one of a bacterium cellulolitic production that have ability to degrade cellulose for produce cellulase enzymes. The aims of the present study is to determine the type of bacteria produced cellulase enzymes in the rumen of cattle aceh. The diversity of cellulolitic bacteria is determined by analysis of 16S rRNA genes. Isolation of cellulolytic bacteria, total DNA isolation, agarose gel electrophoresis, amplification of the 16S rRNA gene, determining the DNA sequence, DNA sequence homology and alignment of DNA sequences. Isolation of bacterium cellulolitic, isolating total DNA, gel electroforesis agarosa, amplified of gen 16S rRNA, determination the DNA barcode, homology DNA barcode, and alignment of DNA sequences. The sample of rumen liquid was taked from RPH , then maked isolation of total DNA to get pure DNA from bacterium whos result and amplification DNA of bacterium cellulolitic separated by electroforesis gel agarosa. Gene of bacterium cellulolitic was isolated based Polymerase Chain Reaction methode. Alignment is done by using BlastN program, then use CLustalW program. It can be concluded that the species of bacteria (FKH_USK_6) in rumen has a closeness with Coronabacter sakazakii bacteria strain PNP8 of 97% according to the sizeof the DNA sequence and the same position on an existing gene in GenBank.

20. The Isolation of Salmonella sp. on quail eggs (cortunxi-cortunix japonica) that failed to hatch in Garot, Darul Imarah Subdistrric, Aceh Besar Latifa Suryandari; Erina Erina; Darniati Darniati; Safika Safika; Nuzul Asmilia; M. Nur Salim
Jurnal Medika Veterinaria Vol 12, No 2 (2018): J. Med. Vet.
Publisher : Universitas Syiah Kuala

The study aimed to isolate the Salmonella sp on quail eggs that failed to hatch in Garot village, Darul Imarah Subdistrict, Aceh Besar. A total of thirty quail eggs that failed to hatch were examined in microbiology laboratory of Veterinary Medicine Faculty, Syiah Kuala University. Modification of Carter method was used in this study to isolated samples. The eggs were opened for taking the embryo and then it was swabbed with sterile swabs. The swab was put into Selenite Cystine Broth (SCB) and incubated at 37o C for 24 hours. The breed was then transferred onto Salmonella Shigella Agar (SSA). The growing Bacteria were observed for their morphology and Gram staining to make sure the bacteria belonged to the negative Gram. The result showed that 10 of the 30 of quail eggs that failed to hatch were positively infected with Salmonella sp. Based on this study, it can be concluded that the salmonella sp.can be isolated from quail eggs which were not hatched in the farm of Garot, Darul Imarah Subdistrict, Aceh Besar

Antibiotic Resistant Pattern and Resistant Gene Identification of Staphylococcus aureus from Chicken Farm in Bogor Nabila Swarna Puspa Hermana; Usamah Afiff; Safika Safika; Agustin Indrawati; Fachriyan Hasmi Pasaribu
Jurnal Veteriner Vol 22 No 2 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Chicken is one of the important protein source in Indonesia. Moreover, the largest population of chicken layer and poultry in Indonesia is known situated at West Java province with Bogor manicipality as the main producer. The aims of this study were to determine the antibiotic resistance pattern of Staphylococcus aureus isolated from poultry and layer farm in Bogor. The study also identified gene encoded the resistance. Cloacal swab samples were collected from chicken broiler and layer farm in Bogor manicipality. The samples were then cultured in Mannitol Salt Agar (MSA) medium to obtain S. aureus. Suspected colony was then confirmed by biochemical test. Positive strains were tested against several antibiotics and the diameter of clear zone arround of colony was interpreted based on Clinical and Laboratory Standard Institute. Furthermore, the DNA from resistant strains were then extracted, followed by detection of the resistance gene by using polymerase chain reaction (PCR) method. A total of 14 isolates of S. aureus were positive from poultry farm, and 15 isolates from layer farm. Most of all were resistant to tetracycline, ampicillin, oxytetracycline, erythromycin and nalidixic acid. On the other hands, several strains were sensitive to gentamycin and chloramphenicol. The study showed 28 isolates out of them were multi-drug resistant. Resistant gene such as blaTEM, gyrA and tetA were also identified in some isolates except for ErmB gene which was found in isolates originated from poultry farm. In conclussion, S. aureus in both farm showed mostly multi-drug resistant to several antibiotics which were supported by identification of resistant gene among isolates.

Jumlah Koloni Bakteri Selulolitik pada Sekum Ayam Kampung (Gallus domesticus) raudhatul jannah jannah; Safika Safika; Muhammad Jalaluddin
JURNAL ILMIAH MAHASISWA VETERINER Vol 1, No 3 (2017): MEI - JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Penelitian ini bertujuan menghitung jumlah koloni bakteri selulolitik pada sekum ayam kampung. Sampel yang digunakan yaitu isi sekum ayam kampung yang diambil di Tempat Pemotongan Unggas (TPU) Lambaro Aceh Besar sebanyak 5 ekor ayam kampung. Sampel dilakukan pengenceran 10-2 sampai 10-5, kemudian diinokulasi ke dalam media BHM-CMC agar dengan metode tuang (pour plate). Selanjutnya, diinkubasi dengan suhu 37˚C selama 96 jam. Koloni bakteri selulolitik yang tumbuh kemudian dilakukan pengamatan morfologi koloni (warna, posisi, pinggiran, dan diameter koloni), diameter zona bening, penghitungan jumlah total bakteri, dan pewarnaan Gram. Hasil penelitian menunjukkan bahwa koloni bakteri berwarna putih, berada di dalam dan di permukaan agar, memiliki pinggiran rata, diameter koloni 2-3 mm dan diameter zona bening 18 mm dan 29 mm. Bakteri berbentuk basil bereaksi negatif terhadap pewarnaan Gram, sedangkan bakteri berbentuk kokus bereaksi positif dan negatif terhadap pewarnaan Gram. Jumlah total bakteri pada isi sekum ayam kampung yaitu 5.3 x 105 cfu/g.Kata kunci: Ayam kampung, bakteri selulolitik, sekum, Carboxy Methyl Cellulase. The aim of this study was to count the total count of cellulolytic bacteria colony in caecum of native chicken. The sample used was caecum contents of native chicken which taken from the poultry slaughterplace at Lambaro Aceh Besar. Sample were diluted from 10-2 to 10-5, then inoculated to agar BHM-CMC media with pour plate method. Then, incubated with temperature 37oC about 96 hours. A colony of cellulolytic bacteria which were grown then were observed by using colony bacteria criteria (colour, position, edge, and diameter of the colony), diameter of the bacterial zone, counting a total of bacteria which was grown, and Gram stain. The results showed that there is white bacterial colony, located inside and surface agar, has flat periphery, diameters of colony 2-3 mm and diameters of bacterial zone 18 and 29 mm. Bacill-shaped bacteria react negative to Gram staining, whereas coccus-shaped bacteria react positive and negative to Gram staining. Number of total bacteria in the native chicken’s caecum was 5.3 x 105 cfu/g Keywords: Native chicken, cellulolytic bacteria, caecum, Carboxy Methyl Cellulose.

JUMLAH KOLONI BAKTERI SELULOLITIK PADA ILIUM AYAM KAMPUNG (Gallus domesticus) nurjannaini nurjannaini; safika safika; M. Jalaluddin M. Jalaluddin
JURNAL ILMIAH MAHASISWA VETERINER Vol 1, No 3 (2017): MEI - JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Penelitian ini bertujuan menghitung jumlah koloni bakteri selulolitik pada ilium ayam kampung. Sampel yang digunakan yaitu isi ilium 5 ekor ayam kampung yang diambil di Tempat Pemotongan Unggas (TPU) Lambaro Aceh Besar. Sampel yang diperoleh dilakukan pengenceran 10-2 sampai 10-5, kemudian diinokulasi ke dalam media BHM-CMC agar dengan metode tuang (pour plate). Selanjutnya, diinkubasi dengan suhu 37˚C selama 96 jam. Koloni bakteri selulolitik yang tumbuh kemudian dilakukan pengamatan morfologi koloni (warna, posisi, pinggiran, dan diameter koloni), diameter zona bening, penghitungan jumlah total bakteri dan pewarnaan Gram. Hasil penelitian menunjukkan bahwa terdapat morfologi koloni berwarna putih bening, posisi di dalam dan di permukaan agar, pinggiran bergerigi, diameter koloni 2-4 mm dan zona bening 19 mm sampai 26 mm. Bakteri selulolitik berbentuk basil Gram negatif dan kokus Gram positif. Rata-rata jumlah total bakteri pada isi ilium ayam kampung yaitu 3,3 x 105 cfu/g. The aim of this study was to count the total sum of cellulolytic bacteria colony in the illium of native chicken. The sample used was illium contents of native chicken whic taken from the poultry slaughterhouse at Lambaro Aceh Besar. Sample were diluted from 10-2 to 10-5, then inoculated to agar BHM-CMC media with pour plate method. After that, incubated with temperature 37oC about 96 hours. A colony of cellulolytic bacteria which were grown then were observed by using colony bacteria criteria (colour, position, edge, and diameter of the colony), diameter of the bacterial zone, counting a total of bacteria which was grown, and Gram stain. The results showed that there is white translucent colony morphology, position inside and surface agar, cogged periphery, diameters of colony 2-4 mm and bacterial zone 19 to 26 mm. Cellulolytic bacteria Gram-negatif bacilli, Gram-negative cocci and Gram-positive cocci. The average number bacteria of total bacteria in the native chicken’s illium was 3.3 x 105 cfu/g.

Identifikasi Lactobacillus sp pada Orangutan Sumatera (Pongo abelii) Liar Menggunakan KIT API 50 CHL di Stasiun Penelitian Suaq Belimbing Aceh Selatan Wardinal Wardinal; Safika Safika; Yulia Sari Ismail
Biotik Vol 7, No 1 (2019): JURNAL BIOTIK
Publisher : Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/biotik.v7i1.5472

Lactic Acid Bacteria (LAB) has many benefits for human and animal health and has been widely used as a probiotic. One of the LAB is the genus Lactobacillus which consists of many species used for fermentation and food preservation. This study was conducted to isolate and identify the LAB of the genus Lactobacillus from the faeces of wild Sumatran Orangutan (Pongo abelii) at the Suaq Belimbing Research Station in South Aceh. Bacterial isolation was carried out using Man Rogosa Sharpe Agar (MRSA). Colonies that grew on MRSA media were observed for morphology and were Gram stained. Biochemical tests were conducted using KIT API 50 CHL. Data analysis used the Apiweb computer program Version V-5.2. The results showed that the OUL isolate was a species of Lactobacillus delbrueckii ssp delbrueckii, with an identity of 93.8%. Based on this, it can be concluded that there is a Lactic Acid Bacteria of the Lactobacillus in the faeces of wild Sumatran Orangutan (Pongo abelii) at the Suaq Belimbing Research Station in South Aceh.

6.Detection of Escherichia coli Contamination in Vaname Shrimp (Litopenaues vannamei) at Fish Auction Place (TPI) Lampulo Banda Aceh Wilyo Hendro Fauzan; Wahyu Eka Sari; Andi Novita; Faisal Jamin; Razali Daud5; Rastina Rastina; Safika Safika
Jurnal Medika Veterinaria Vol 15, No 2 (2021): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v15i2.26075

Shrimp is an easily polluted material, so it requires good handling. Contamination can occur during harvest, handling, or transportation. This study aims to detect Escherichia coli contamination in vaname shrimp sold at TPI Lampulo Banda Aceh. A total of 10 samples of vaname shrimp were obtained from 10 merchants at TPI Lampulo. The research method used is observational, and the data analysis is done descriptively. The detection of E. coli contamination was carried out by the total plate count (TPC) test method. Based on the study results, two of the 10 samples of vaname shrimp merchant detected E. coli bacterial contamination, namely the results of sample 01 merchant indicated the number of TPC 2x102 CFU/g and in 09 merchants indicated the number of TPC 11x101 CFU/g. The TPC results were indicated by the growth of E. coli colonies on eosin methylene blue agar (EMBA) media after incubation for 24 hours at 37 ºC. The number of the two detected positive samples showed less than 1x103 CFU/g by the provisions of SNI 2332.3:2015. Thus, it can be concluded that E. coli contamination was detected in vaname shrimp sold by the merchant at TPI Lampulo but did not exceed the SNI threshold, so it was safe for consumption.

Detection of Tetracycline Resistance Genes among Escherichia coli Isolated from Layer and Broiler Breeders in West Java, Indonesia A. Indrawati; K. Khoirani; S. Setiyaningsih; U. Affif; Safika; S. G. Ningrum
Tropical Animal Science Journal Vol. 44 No. 3 (2021): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar

A major current problem in public health is the issue of antimicrobial resistance of Escherichia coli in humans and poultry. In Indonesia, multidrug-resistant E. coli are of specific concern since such E. coli may cause public health problems in humans. The prevalence of multidrug-resistant chicken E. coli strains and the E. coli resistance genes, which are tet(A) and tet(B) genes, were investigated in the present study. A total of 57 swabs were collected from layer and broiler breeder farms in West Java, Indonesia, and used in the experiment. Eighteen isolates were identified as E. coli by the disk diffusion method. The isolates classified as drug-resistant and intermediate were then identified using PCR for the antimicrobial resistance genes. The results showed that 18 isolates of E. coli from layer-breeder and broiler-breeder farms in West Java were resistant to ampicillin (100%), nalidixic acid (94%), tetracycline (88%), oxytetracycline (83%), gentamicin (27%), and chloramphenicol (22%). PCR identification of E. coli antimicrobial-resistant genes in 18 isolates showed tet(A) and tet(B) genes. This study reports antimicrobial resistance genes among E. coli on layer and broiler breeder farms in West Java. This present study showed that E. coli isolated from layers-breeder and broiler-breeder farms in West Java of Indonesia carried tet(A) and tet(B) genes, the multidrug-resistance genes.

Sensitivitas antimikroba dari bakteri terisolasi yang paling umum pada Feline Upper Respiratory Infection Karen Lee; Usamah Afiff; Safika Safika; Titiek Sunartatie
ARSHI Veterinary Letters Vol. 5 No. 3 (2021): ARSHI Veterinary Letters - Agustus 2021
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.5.3.55-56

Pada kucing, infeksi saluran pernapasan atas (URI) dapat dikaitkan dengan infeksi bakteri primer atau sekunder dan umumnya dilakukan pengobatan dengan antimikroba. Penggunaaan antimikroba yang tidak tepat, dan penggunaannya berlebihan. Tidak ada protokol rinci untuk pengobatan, seperti yang tersedia untuk pengobatan manusia. Tujuan penelitian ini adalah untuk mengetahui kepekaan antimikroba dari bakteri yang diidentifikasi pada kucing yang terinfeksi URI. Dilakukan isolasi dan identifikasi bakteri pada sampel kucing yang terinfeksi URI kemudian dilanjutkan dengan uji kepekaan antibiotik amoksisilin, doksisiklin, tetrasiklin, azitromisin, siprofloksasin, dan sefotaksim dengan metode difusi Kirbey Bauer Agar Dics. Berdasarkan hasil penelitian, bakteri yang dapat diidentifikasi adalah Enterobacter spp, Streptococcus spp, Staphylococcus spp, Pseudomonas spp, Seratia spp, Yersinia spp, Micrococcus spp, Klebsiella spp dan Hafnia spp. Ditemukan resistansi antibiotik amoksisilin pada empat isolat Staphylococcus spp dan dua isolat resistan terhadap sefotaksim. Resistansi antibiotik amoksisilin dan tetrasiklin ditemukan pada satu isolat Streptococcus spp, dua isolat resistan terhadap sefotaksim serta dua isolat intermediet terhadap doksisiklin. Satu isolat bakteri Enterobacter spp resistan terhadap amoksisilin, azitromisin dan dua isolat resistan terhadap sefotaksim. Semua isolat yang diuji sensitif terhadap siprofloksasin. Berdasarkan uji kepekaan antibiotik, sebagian besar isolat bersifat sensitif, namun terdapat ada isolat yang resistan terhadap antibiotik, terutama amoksisilin dan sefotaksim.

Kelimpahan Fungi Kelas Dothideomycetes Pada Lumba-lumba Hidung Botol (Tursiops aduncus) Kurnia Tiara Aulia; Agustin Indrawati; Safika
Acta VETERINARIA Indonesiana Vol. 10 No. 2 (2022): Juli 2022
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.10.2.148-156

Mamalia laut memainkan peran ekologis penting di lautan, dan menjadi prioritas global karena kepekaan terhadap perubahan lingkungan. Lumba-lumba hidung botol (Tursiops aduncus) merupakan salah satu mamalia laut yang tersebar luas di Indonesia. Informasi mengenai mikrobioma masih sangat sedikit diketahui, terutama informasi mengenai mikrobioma fungi. Penelitian ini dilakukan untuk mengidentifikasi kelimpahan fungi kelas Dothideomycetes pada saluran pencernaan khususnya usus lumba-lumba hidung botol (Tursiops aduncus) dengan menggunakan Platform Next Generation Sequencing (NGS). Penelitian ini berhasil mengidentifikasi 6 genera dari 4 ordo yang yang meliputi Neodevriesia, Altenaria, Stemphylium, Phaeophleospora, Diplodia dan Venturia. Kelompok Neodevriesia adalah yang paling mendominasi sebesar 62%, diikuti Alternaria 16% dan Phaeophleospora 14%. Fungi yang tidak teridentifikasi mencapai 5%. Kelimpahan fungi pada usus lumba-lumba hidung botol (Tursiops aduncus) diasumsikan memiliki korelasi dengan lingkungan dan makanan lumba-lumba pada pusat konservasi.

Co-Authors . Darniati A. Indrawati Abdul Harris Agustin Indrawati Akhmaloka Akhmaloka Al Azhar Andi Novita Aprilia Hardiati Azmansyah Azmansyah Cut Lathifa Nurrady Darmawi Darmawi Darmawi Darmawi Darmawi Darmawi Darmawi Darmawi Erdiansyah Rahmi Erdiansyah Rahmi Erina Erina Erina Erina Erina Erina F Madayanti, F FACHRIYAN HASMI PASARIBU Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Faisal Jamin Faisal Jamin Fakhrurrazi Fakhrurrazi Fakhrurrazi Fakhrurrazi FAUZAN ARISANDI Fauzan Arisandi Fitria Sari Ramadhani Gressha Vionalle Ademi I wayan Teguh Wibawan Ichsan Maulana Indah Melzana Ismail Ismail Juliadi Ramadhan K. Khoirani Karen Lee Kurnia Tiara Aulia Latifa Suryandari M Jalaluddin M. Jalaluddin M. Jalaluddin M. Nur Salim Mahdi Abral Maryulia Dewi Moliwati Moliwati Muhammad Jalaluddin Muttaqien Muttaqien Nabila Swarna Puspa Hermana Ni Luh Putu Ika Mayasari Nurhaspika Nurhaspika nurjannaini nurjannaini NURLIANA NURLIANA Nuzul Asmilia P Aditiawati Rastina Rastina raudhatul jannah jannah Razali Daud5 Rinidar Rinidar S. G. Ningrum S. Setiyaningsih Sari, Wahyu Eka Siti Aisyah Siti Hajar Siti Wardianah Matondang T. Reza Ferasyi Titiek Sunartatie Tongku N Siregar U. Affif Ulfi Widi Arsih Usamah Afiff Wardinal Wardinal Wenny Novita Sari Wenny Novita Sari Wilyo Hendro Fauzan Yamin Yaddi Yandi Syahputra Yulia Sari Ismail Zumala Nilasari

Title

Found 34 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 34 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 34 Documents
Search