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Effect of PMSG administration in combination with vaginal sponge on estrous occurrence and litter size of Javanese sheep Setiawan, Rangga; Rasad, Siti Darodjah; Solihati, Nurcholidah; Widyastuti, Rini; Soeparna, Soeparna
Jurnal Kedokteran Hewan Vol 11, No 4 (2017): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i4.4349

Abstract

AbstractThe use of vaginal sponge in estrous synchronization has been a popular method to uniform estrous cycle of small ruminants. In order to increase the litter size, the use of the sponge is combined with PMSG. Therefore the objective of this study was to evaluate the effect of PMSG in combination with vaginal sponge on estrous response and litter size of Javanese local sheep. The study was conducted in Faculty of Animal Husbandry, Padjadjaran University, Indonesia with 32 non-pregnant local sheep. All these sheep was divided into four groups of treatment, i.e: 1) group of sheep was subjected to vaginal sponge (VS) treatment containing 30 mg Medroxyprogesterone acetat (MAP) inserted in the vagina for 14 days; 2) group of sheep was subjected to the 30 mg MAP in VS plus 300 IU PMSG injection -1 d before VS removal; 3) group of sheep was subjected to the 30 mg MAP in VS plus 300 IU PMSG injection at the day of VS removal; 4) group of sheep was subjected to the 30 mg MAP in VS plus 300 IU PMSG injection +1 d after VS removal. The result showed that all treatment groups had 100%  of estrous response. However, the occurrence of estrous time varied among group after VS removal. Group 1 (G1) and G4 tend to have estrous occurrence at day 3 after VS removal by 55% and 75% response, respectively.  Whilst, G2 and G3 tend to have estrous occurrence respectively at day 1 (62,5%) and day 2 (50%) after VS removal. A 100% of pregnancy rate occurs in G2 and G4, while G1 and G3 have a 88,9% and 87,5%, respectively. No significant effects of the treatments on the ewes litter size. In conclusion, the use of PMSG administration 24 h prior, after, or at sponge withdrawal increase a tighter synchrony of oestrous and pregnancy rate, but has no effect on the increasing of litter size in Javanese local sheep.Keywords: Vaginal sponge, PMSG, estrous response, pregnancy rate, litter size, sheep
Mice Oocytes Respond after Vitrification Followed by Artifical Activation Using a Various Concentration of Strontium Chloride and Cytochalasin B Ridlo, Muhammad Rosyid; Widyastuti, Rini; Lubis, Alkaustariyah; Fahrudin, Mokhamad; Boediono, Arief
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (101.945 KB)

Abstract

Oocyte vitrification is the important part of gamete preservation for further purpose. The objective of this study was to evaluate the response or development of vitrified-mice oocyte following activation using various concentrations of Strontium Chloride (Sr Cl2). Oocytes were collected from superovulation-induced female mice. Oocytes vitrification was then performed using a gradual equilibration of 2 M Ethylene Glycol in 0.25 M sucrose and 7 M Ethylene Glycol on 0.5 sucrose. Subsequently, the vitrified oocytes were thawed and activated using various Strontium Chloride concentration in each group. Control 1 is unvitrified oocyte and without Sr Cl2. Control 2 is unvitrified oocyte then activated by 20 mM Sr Cl2. Zero (0) mM Sr Cl2 is vitrified oocyte without Sr Cl2. Group Ten (10) mM is vitrified oocyte then activated by 10 mM Sr Cl2. Group Twenty (20) mM is vitrified oocyte then activated by 20 mM Sr Cl2. The viability of vitrified-thawed oocytes was observed based on ooplasm integrity. Whereas the oocytes respond to artificial activation was observed based on pronucleus formation after 10 hours of activation. The result showed that 39% of oocyte degenerated following vitrification. The respond of vitrified-thawed oocytes following artificial activation using Strontium Chloride was significantly lower compared to fresh oocytes (p<0,05). Interestingly the highest percentage of activated oocytes (36.36%) was present in a group achieved 20 mM Strontium Chloride. As conclusion is Strontium Chloride 20mM has a best result (36,36%) to activate vitrified oocyte than 0 mM and 10 mM of Strontium Chloride.
Testes and body weight alteration related cardamom extract administration in mice Hartady, Tyagita; Praditha, Arvia Nisrina; Faza, Nabila Ayu; Widyastuti, Rini
ARSHI Veterinary Letters Vol. 3 No. 1 (2019): ARSHI Veterinary Letters - Februari 2019
Publisher : Faculty of Veterinary Medicine, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (613.184 KB) | DOI: 10.29244/avl.3.1.7-8

Abstract

Cardamom is known as a plant with millions of benefits and it is known to contain aphrodisiac substances. The role of cardamom as an aphrodisiac need to be studied more deeply from the scientific point of view. The experimental object is a 3-month-old male white mice (Mus musculus albinus) with an average weight of about 30-35 grams. After being acclimatized for approximately 2 weeks, 35 mice were divided into 3 groups based on the dose of cardamom extract. At the end of the experiment, the mice will be sacrificed and then the testes were weighed and cauda epididymis were isolated to collect the sperm. Our findings suggested that the dose of administration has important role in affecting the weight of testes and bodies of mice. However, further studies on sperm profiles and optimal administration doses of cardamom extract are highly recommended.
Low Concentration of Ethylene Glycol Improved Recovery Rate of Human Spermatozoa After Vitrification (ETILEN GLIKOL KONSENTRASI RENDAH MENINGKATKAN RECOVERY RATE SPERMATOZOA MANUSIA PASCAVITRIFIKASI) Widyastuti, Rini; Sumarsono, Sony Heru; Boediono, Arief; Rasad, Siti Darodjah
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The use of cryoprotectants for the cryopreservation of human spermatozoa, oocytes, zygote, earlycleavage stage of embryos and blastocyst is an integral part of almost every human In Vitro Fertilizationprogram. Moreover, the cryopreservation of these types of cells by direct plunging into liquid nitrogen (-196°C) usually requires a high concentration of cryoprotectant with a consequent of cytotoxic effect. Theaim of this study was to observe the effect of ethylene glycol concentration on the spermatozoa recoveryrate following vitrification process. Earle’s balanced salt solution + 0.25 M sukrosa + 1 % human albumineserum as basic solution supplemented with some different concentrations of etylene glycol (ie: 36.25%;18.25%; 9.12%; 4.56%; 1.14% and 0.57%) were used to evaluate the motility and viability of spermatozoafollowing vitrification. Human’s spermatozoa from ejaculates with progressive motility and viability above50% were used as samples. Samples were mixed with vitrification solution and then loaded into 0.25 mLstraws, equilibrated for 10 minutes at room temperature before plunged into liquid nitrogen. Spermatozoathawing was done at 24 hours after the vitrification. The results showed that, the decrease of spermatozoamotility and viability were observed at the highest (100%, 96.70%, respectively) in the samples that wereadded with vitrification medium contained 36.25% of ethylene glycol. On the other hand, the decrease ofthe spermatozoa motility and viability were found at the lowest (14.11%, 43.81 %, respectively) in thesamples without ethylene glycol supplementation. It can be concluded that the highest spermatozoa recoveryrate was obtained from the vitrification using a low concentration of ethylene glycol.
Anemia and Eosinophilia in Traditional Goat Farmers: Early Markers of Strongyle Zoonoses (ANEMIA DAN EOSINOFILIA PADA PETERNAK KAMBING TRADISIONAL: PENANDA AWAL ZOONOSIS STRONGYLUS) Taufik, Insy Nafisah; Heryaman, Henhen; Widyastuti, Rini; Syamsunarno, Mas Rizky A. A.; Ghozali, Mohammad
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The spread of zoonoses between humans and animals can increase with the presence of specific contacts between the two. As well as intensive contact between farmer and goat. Anemia is a common hematologic change encountered in infection and zoonoses so it can be used as an early indicator for zoonoses. Nevertheless, anemia itself cannot lead to a specific etiology thus differential leukocyte count and goat fecal examination can be a good supporting test. Understanding the etiologies of the disease is critically important in preventing the decline in human’s quality of life as well as economic losses. This cross-sectional study included 30 farmers who were recruited by purposive sampling method. They agreed to do a CBC examination with 22 parameters. From the test, 37% of them showed mild anemia. There were 45% farmers with microcytic hypochromic anemia while the rest had normocytic normochromic anemia. There was an increased number in eosinophil and segmented neutrophil from anemic farmers, 18% and 9% respectively. Besides, 13% of goats physical examination indicated as below normal conditions characterized by lean bodies, lymph node swelling, and pale mucosa. Whereas 38% goat’s fecal test showed evidence of Strongyle eggs. The research suggests a possible interrelation between animals, humans, and the environment with an increased risk of zoonoses. Anemia and eosinophilia that occur can be an early marker which will be decisive not only for the success of the disease progression reduction but also the long term suppression of the economic loss.
The Effect of Ethanol Extract of Cogon Grass Root (Imperata cylindrica) to Estrus Cycle and Metabolite Profile in Female Mice Robianto, Sondi; Syamsunarno, Mas Rizky Adipurna Anngun; Lubis, Alkaustariyah; Priscilia, Madeleine; Anggraeni, Neni; Ghozali, M.; Ritongga, Mulyanusa; Widyastuti, Rini
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.687 KB) | DOI: 10.19087/jveteriner.2019.20.2.196

Abstract

The use of herbs as contraception has become the focus of current contraceptive development.. In the previous study, the extract ethanol of cogon grass roots (Imperata cylindrica) has the effect of decreasing sperm production and changing metabolite profiles in male mice. However, the effect of this Indonesian well-known herb is still indefinite in female.The purpose of this study was to investigate the effect of ethanol extract of cogon grass roots to the fertility of female mice. The study was conducted at Animal Laboratory of Faculty of Medicine and Central laboratory Universitas Padjadjaran from November to December 2017. Mice were given ethanol extract of cogon grass roots 90 and 115 mg/kg body weight per oral. After three weeks, vaginal cytology was observed and metabolite profiles were measured. The results showed a shortening of the estrus phase in 90 and 115 mg/kg treatment group. However, the level of glucose and cholesterol in serum were not different among groups. This study indicated the extract ethanol of cogon grass roots has potential effect as contraceptive agent and the effect is independent from metabolite profiles. Keywords: Cogon Grass, Imperata cylindrica, Estrus Cycle Phase, Metabolite Profile
Oral Administration of Cogongrass (Imperata cylindrica L) Root Ethanol- Extract causes Mouse Epididymal Sperm Abnormality (PEMBERIAN EKSTRAK ETANOL AKAR ALANG-ALANG (IMPERATA CYLINDRICA L) SECARA ORAL MENYEBABKAN ABNORMALITAS SPERMA EPIDIDYMIS MENCIT) Widyastuti, Rini; Sudiman, Jaqueline; Tyagita, Tyagita; Syamsunarno, Mas Rizky Adipurna Anggun; Sumarsono, Sony Heru
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (115.412 KB)

Abstract

Sperm morphology is an important parameter to be observed in the male fertility. Some of the bioactive compounds of cogongrass root such as alkaloid and terpenoid, affect male fertility by interference the spermatogenesis. The objective of the study was to observe the effect of cogongrass root ethanol extract on mouse sperm morphology. This study was carried out by oral administration of two different doses i.e 90 and 115 mg/kg body weight of cogongrass root ethanol extract into 8-10 weeks old DDY strain mice for 14 days to evaluated the acute effect due to the administration of cogongrass root ethanol extract on mouse sperm morphology. The results showed that treatment with cogongrass root ethanol extract significantly increased sperm abnormalities followed a dose depending pattern (p<0.05). Interestingly, the administration of cogongrass root extract did not affect sperm head morphology but tailless, folded and bent sperm increased linearly with the administration dose of cogongrass root ethanol extract. In conclusion, cogongrass root ethanol extract causes secondary sperm abnormalitties on mouse sperm.
Tingkat Pengetahuan dan Respon Peternak Kambing Perah terhadap Penyakit Hewan Studi Kasus: Kelompok Tani “Simpay Tampomas” Cimalaka, Sumedang Widyastuti, Rini; Wira, Dwi Wahyudha; Ghozali, Mohammad; Winangun, Kikin; Syamsunarno, Mas Rizky Anggun Adipurna
Dharmakarya Vol 6, No 2 (2017): Juni
Publisher : Universitas Padjadjaran

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Abstract

Peternakan kambing perah merupakan mata pencaharian utama masyarakat Kecapatan Cimalaka, Kabupaten Sumedang. Pengelolaan peternakan masih dilakukan dengan cara tradisiona, sehingga perlu dilakukan upaya peningkatkan pengetahuan kelompok peternak kambing perah mengenai pengelolaan manajemen kesehatan kambing perah serta mencegah terjadinya kerugian akibat dampak penyakit.  Salah satunya adalah melalui kegiatan penyuluhan mengenai penyakit-penyakit pada ternak terutama dari aspek klinis. Kegiatan diawali dengan survei lokasi, pemberian vitamin pada kambing perah, penyuluhan, pengisian kuesioner, pengolahan hasil kuesioner. Pada tahap akhir, dilakukan timbal balik (feedback) pada peternak atas hasil yang didapatkan dari pengobatan dan kuesioner. Hasil menunjukkan bahwa tangka pengetahuan peternak terhadap penyakit hewan dan cara pencegahannya sudah cukup baik. Kasus yang banyak berkembang di daerahpeternakan tersebut adalh Scabies, mastitis dan Bloat dengan gejala umum berkurangnya nafsu makan dan demam. Peternak biasanya memberikan pertolongan pertama dengan memberikan air asam dan obat cacing. Berdasarkan hasil tersebut, dapat bahwa peternak telah memiliki tingkatpengetahuan penyakit yang baik tetapi belum memiliki pengetahuan untuk penangann penyakit secara memadai. 
Development of Product-Based Job Sheet as Instructional Media in Vocational Education Widyastuti, Rini; Utami, Iga Setia
Journal of Educational Science and Technology (EST) Volume 4 Number 2 August 2018
Publisher : Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (991.562 KB) | DOI: 10.26858/est.v4i2.6064

Abstract

The purpose of this research is to produce a product-based job sheet that can be used as an instructional media on practicum activities. In this study also tested the validity of the job sheet developed. The research method used is research development with a 4D model consisting of define, design, development and disseminate. The research instrument used is a validation sheet consisting of content validation, format, and presentation of the job sheet which is analyzed by descriptive analysis technique. The results showed that 1) the product-based job sheet developed was highly valid on the content/content aspects, 2) the product-based job sheet developed valid on the aspect of the format, and 3) the product-based job sheet developed was valid in the presentation aspect. Based on the results of the study can be concluded that the product-based worksheet developed valid for use in Object Oriented Programming subject. 
APLIKASI KRIOPROTEKTAN EKSTRASELULER TUNGGAL SECARA EFEKTIF MEMPERTAHANKAN KUALITAS SPERMA MANUSIA PASCAVITRIFIKASI Widyastuti, Rini; Syamsunarno, Mas Rizky A. A.; Ghozali, Mohammad
Majalah Kedokteran Bandung Vol 50, No 4 (2018)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (176.359 KB) | DOI: 10.15395/mkb.v50n4.1319

Abstract

Pemilihan jenis krioprotektan merupakan salah satu kunci keberhasilan dalam mempertahankan motilitas dan viabilitas sperma pascavitrifikasi. Secara konvensional, vitrifikasi menggunakan konsentrasi krioprotektan dan laju kecepatan pembekuan yang tinggi untuk menghindari pembentukan kristal es intra dan ekstraseluler yang menyebabkan kerusakan dan kematian pada sel. Berdasar atas kemampuan menembus membran sel, krioprotektan dibedakan menjadi krioprotektan ekstra dan intraseluler. Sperma manusia memiliki struktur morfologi yang sangat padat dan sedikit mengandung sitoplasma sehingga perpindahan cairan selama proses vitrifkasi sangat kecil. Selain itu, sperma manusia juga mengandung beberapa jenis protein yang dapat berfungsi sebagai krioprotektan intraseluler. Berdasar atas kondisi tersebut, penggunaan krioprotektan pada vitrifkasi sperma manusia memerlukan studi lebih lanjut. Penelitian ini dilakukan di Laboratorium Sentral Universitas Padjadjaran dan dilaksanakan pada bulan Desember 2017?Januari 2018.Penelitian ini bertujuan mengetahui motilitas dan viabilitas sperma pascavitrifikasi dengan menggunakan tipe krioprotektan yang berbeda dan kombinasi keduanya. Earle?s balanced salt solution digunakan sebagai krioprotekan ekstraseluler, sementara EG 0,57% sebagai krioprotektan intraseluler. Sampel yang telah ditambahkan medium vitrifikasi diequilibrasi selama 10 menit, kemudian dikemas di dalam straw 0,25 mL dan langsung dipaparkan ke dalam nitrogen cair. Evaluasi dilakukan dengan melakukan thawing setelah 24 jam penyimpanan. Hasil menunjukkan bahwa motilitas dan viabilitas sperma tertinggi pada kelompok yang menggunakan media vitrifikasi krioprotektan ekstraseluler, walaupun secara statistik tidak berbeda nyata dengan kelompok lainnya (34%; 50%; p<0,05). Sebagai simpulan, krioprotektan ekstraseluler merupakan media vitrifikasi terbaik untuk menjaga motilitas dan viabilitas sperma pascavitrifikasiKata kunci: Krioprotektan, sperma manusia, vitrifikasi  Single Extracellular Cryoprotectant Application Effectively Maintain Post-Vitrification Human Sperm QualitySelection of cryoprotectant is one of the keys to maintain sperm motility and viability after vitrification. Conventionally, vitrification uses cryoprotectants with high concentration and cooling rate to avoid the formation of intra- and extra-cellular ice crystals that can induce cell damage and cell death. Morphology structure of human sperm is very dense and contain less cytoplasm compartment; therefore mobilization of fluid is minimum during vitrification. In addition, human sperm also contains several types of protein that function as intracellular cryoprotectants. Based on this condition, the use of cryoprotectants in human sperm vitrification needs a further study. This study was conducted at the Central Laboratory of Universitas Padjadjaran in December 2017?January 2018. The aim was to determine the motility and viability of post-vitrification sperm by using different types of cryoprotectants. Samples that had been mixed with vitrification medium were equilibrated for 10 minutes, packed in a 0.25 mL straw, and directly exposed to liquid nitrogen. The evaluation was conducted  by thawing after 24 hours of storage. The results showed that the highest sperm motility and viability was  found in the group that used extracellular cryoprotectant vitrification media although it was not statistically different ( 34%; 50%, p <0.05) In conclusion, extracellular cryoprotectants are the best vitrification medium for maintaining motility and viability of post-vitrification sperm.Key words: Cryoprotectant, sperm, vitrification