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PENGARUH PEMBERIAN MANUR BROILER DENGAN FERMENTASI Lactobacillus casei TERHADAP KONVERSI PAKAN AYAM KAMPUNG Nururrozi, Alfarisa; Indarjulianto, Soedarmanto; Ramandani, Dhasia; Yanuartono, Yanuartono
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 5, No 2 (2018): December 2018
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

The Effect of Broiler Manure with Lactobacillus casei Fermentation on the Kampung Chicken Feed Convertion Ratio ABSTRACTHusbandry of kampung chicken is constrained by high feed prices and poor productivity. This study aims to utilize alternative feed materials derived from broiler manure to obtain a cheaper feed with good quality. Manure contains high nutrients. Manure was fermented using Lactobacillus casei to improve feed conversion. Two hundred chickens were divided into 4 groups (n = 50). Groups P1, P2, and P3 were given 4%, 8%, and 12% fermentation of L. casei, respectively. Group P0 was given a regular feed without L. casei. Each treatment group consisted of four replicates and were maintained for 60 days. The research design used was Completely Randomized Design subjected to analysis of variant (ANOVA) followed by Duncan test. The feed conversion values of groups P0, P1, P2, and P3 were 4.46; 4.38; 4.21; and 4.54, respectively. The results showed that the feed conversion was not significant in all groups. It was concluded that L. casei fermenter could not improve the feed conversion ratio (FCR).Keywords: FCR, fermentation, kampung chicken, Lactobacillus casei, manure ABSTRAKBudidaya ayam kampung terkendala tingginya harga pakan dan rendahnya produktivitas. Penelitian ini bertujuan memanfaatkan pakan alternatif bersumber manur (limbah kotoran) ayam broiler untuk memperoleh pakan murah dengan kualitas baik. Manur broiler masih mengandung nutrisi yang tinggi. Manur difermentasi menggunakan Lactobacillus casei untuk memperbaiki konversi pakan. Dua ratus ekor ayam dibagi menjadi 4 kelompok (n=50 ekor). Kelompok P1, P2, dan P3 masing-masing diberi ransum yang ditambah fermentasi L. casei sebanyak 4%, 8%, dan 12%. Kelompok P0 diberikan pakan biasa tanpa penambahan L. casei. Setiap kelompok perlakuan terdiri dari empat ulangan dipelihara selama 60 hari. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan analisis ragam yang dilanjutkan dengan uji Duncan. Konversi pakan dari kelompok P0, P1, P2, dan P3 berturut-turut 4,46; 4,38; 4,21; dan 4,54. Hasil penelitian menunjukkan konversi pakan tidak berbeda nyata pada semua kelompok perlakuan. Dari hasil penelitian disimpulkan penggunaan fermenter L.casei pada pakan belum mampu memperbaiki konversi pakan.Kata Kunci: ayam kampung, FCR, fermentasi, Lactobacillus casei, manur

PENGARUH PEMBERIAN MANUR BROILER DENGAN FERMENTASI Lactobacillus casei TERHADAP KONVERSI PAKAN AYAM KAMPUNG Nururrozi, Alfarisa; Indarjulianto, Soedarmanto; Ramandani, Dhasia; Yanuartono, Yanuartono
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 2 (2018): December 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

The Effect of Broiler Manure with Lactobacillus casei Fermentation on the Kampung Chicken Feed Convertion RatioÃ‚Â ABSTRACTHusbandry of kampung chicken is constrained by high feed prices and poor productivity. This study aims to utilize alternative feed materials derived from broiler manure to obtain a cheaper feed with good quality. Manure contains high nutrients. Manure was fermented using Lactobacillus casei to improve feed conversion. Two hundred chickens were divided into 4 groups (n = 50). Groups P1, P2, and P3 were given 4%, 8%, and 12% fermentation of L. casei, respectively. Group P0 was given a regular feed without L. casei. Each treatment group consisted of four replicates and were maintained for 60 days. The research design used was Completely Randomized Design subjected to analysis of variant (ANOVA) followed by Duncan test. The feed conversion values of groups P0, P1, P2, and P3 were 4.46; 4.38; 4.21; and 4.54, respectively. The results showed that the feed conversion was not significant in all groups. It was concluded that L. casei fermenter could not improve the feed conversion ratio (FCR).Keywords: FCR, fermentation, kampung chicken, Lactobacillus casei, manureÃ‚Â ABSTRAKBudidaya ayam kampung terkendala tingginya harga pakan dan rendahnya produktivitas. Penelitian ini bertujuan memanfaatkan pakan alternatif bersumber manur (limbah kotoran) ayam broiler untuk memperoleh pakan murah dengan kualitas baik. Manur broiler masih mengandung nutrisi yang tinggi. Manur difermentasi menggunakan Lactobacillus casei untuk memperbaiki konversi pakan. Dua ratus ekor ayam dibagi menjadi 4 kelompok (n=50 ekor). Kelompok P1, P2, dan P3 masing-masing diberi ransum yang ditambah fermentasi L. casei sebanyak 4%, 8%, dan 12%. Kelompok P0 diberikan pakan biasa tanpa penambahan L. casei. Setiap kelompok perlakuan terdiri dari empat ulangan dipelihara selama 60 hari. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan analisis ragam yang dilanjutkan dengan uji Duncan. Konversi pakan dari kelompok P0, P1, P2, dan P3 berturut-turut 4,46; 4,38; 4,21; dan 4,54.Ã‚Â Hasil penelitian menunjukkan konversi pakan tidak berbeda nyata pada semua kelompok perlakuan. Dari hasil penelitian disimpulkan penggunaan fermenter L.casei pada pakan belum mampu memperbaiki konversi pakan.Kata Kunci: ayam kampung, FCR, fermentasi, Lactobacillus casei, manur

Potensi Bentonite Clay Dan Karbon Aktif Sebagai Aflatoksin Binders Berdasarkan Gambaran Imunologis Dan Histopatologis Pada Ayam Buras Ramandani, Dhasia; Ummami, Risa; Hidayah, Nurulia; Dalimunthe, Naela Wanda Yusria; Indarjulianto, Soedarmanto; Yanuartono, Yanuartono; Nururrozi, Alfarisa
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol 10 No 1 (2020): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Vete
Publisher : Fakultas Peternakan Universitas Papua

Aflatoxin is a toxic compound from the fungus A. flavus which can cause a decrease in chicken productivity. This study aims to evaluate the effect of bentonite clay and activated charcoal in chicken feed as aflatoxin binders based on histopathological and immunological features in chickens. A total of 60 chickens were randomly divided into 4 groups (G0, G1, G2, and G3) each group consisted of 15 chickens. G0 as a control without the addition of aflatoxin to the feed. Groups G1, G2, and G3â€™s feed were added with 106 ppb of aflatoxin. G2 was treated with 5g/kg bentonite clay, G3 was treated with 5g/kg activated charcoal. Chickens were vaccinated with Newcastle Disease (ND) on day 5. On days 14 and 28 serum was collected from the specimens for titer antibody testing. At the end of the study the chickens were dissected, the liver was taken for a histopathological test. The results of the aflatoxin level in the G0, G1, G2, and G3 groups were 10.5, 106, 19.1, and 23.95 ppb, respectively. Microscopic changes show the negative effects of aflatoxin on the liver organ. Group G0 did not show any histopathological changes, Group G2 and G3 showed several mild histopathological changes, whereas group G1 was the most severe. The antibody titers against Newcastle Disease (ND) of 4-weeks old chickens in G0, G1, G2, and G3 were 64, 4, 32, 32 HI units, respectively. The G0 treatment group has a protective titer against ND disease. Group G1 has the lowest (non-protective) ND antibody titer. Whereas the G2 and G3 groups both have protective titers against ND disease. Based on this study it was concluded that the administration of bentonite clay and activated charcoal was able to produce better immunological and histopathological features than the control group.

PENGARUH PEMBERIAN MANUR BROILER DENGAN FERMENTASI Lactobacillus casei TERHADAP KONVERSI PAKAN AYAM KAMPUNG Alfarisa Nururrozi; Soedarmanto Indarjulianto; Dhasia Ramandani; Yanuartono Yanuartono
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 2 (2018): December 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

The Effect of Broiler Manure with Lactobacillus casei Fermentation on the Kampung Chicken Feed Convertion RatioÂ ABSTRACTHusbandry of kampung chicken is constrained by high feed prices and poor productivity. This study aims to utilize alternative feed materials derived from broiler manure to obtain a cheaper feed with good quality. Manure contains high nutrients. Manure was fermented using Lactobacillus casei to improve feed conversion. Two hundred chickens were divided into 4 groups (n = 50). Groups P1, P2, and P3 were given 4%, 8%, and 12% fermentation of L. casei, respectively. Group P0 was given a regular feed without L. casei. Each treatment group consisted of four replicates and were maintained for 60 days. The research design used was Completely Randomized Design subjected to analysis of variant (ANOVA) followed by Duncan test. The feed conversion values of groups P0, P1, P2, and P3 were 4.46; 4.38; 4.21; and 4.54, respectively. The results showed that the feed conversion was not significant in all groups. It was concluded that L. casei fermenter could not improve the feed conversion ratio (FCR).Keywords: FCR, fermentation, kampung chicken, Lactobacillus casei, manureÂ ABSTRAKBudidaya ayam kampung terkendala tingginya harga pakan dan rendahnya produktivitas. Penelitian ini bertujuan memanfaatkan pakan alternatif bersumber manur (limbah kotoran) ayam broiler untuk memperoleh pakan murah dengan kualitas baik. Manur broiler masih mengandung nutrisi yang tinggi. Manur difermentasi menggunakan Lactobacillus casei untuk memperbaiki konversi pakan. Dua ratus ekor ayam dibagi menjadi 4 kelompok (n=50 ekor). Kelompok P1, P2, dan P3 masing-masing diberi ransum yang ditambah fermentasi L. casei sebanyak 4%, 8%, dan 12%. Kelompok P0 diberikan pakan biasa tanpa penambahan L. casei. Setiap kelompok perlakuan terdiri dari empat ulangan dipelihara selama 60 hari. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan analisis ragam yang dilanjutkan dengan uji Duncan. Konversi pakan dari kelompok P0, P1, P2, dan P3 berturut-turut 4,46; 4,38; 4,21; dan 4,54.Â Hasil penelitian menunjukkan konversi pakan tidak berbeda nyata pada semua kelompok perlakuan. Dari hasil penelitian disimpulkan penggunaan fermenter L.casei pada pakan belum mampu memperbaiki konversi pakan.Kata Kunci: ayam kampung, FCR, fermentasi, Lactobacillus casei, manur

MANFAAT SUPLEMENTASI VITAMIN C PADA KESEHATAN TERNAK RUMINANSIA Yanuartono Yanuartono; Alfarisa Nururrozi; Indarjulianto Soedarmanto; Dhasia Ramandani
Jurnal Ilmu dan Teknologi Peternakan Vol. 9 No. 1 (2021)
Publisher : Fakultas Peternakan, Universitas Hasanuddin, Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jitp.v9i1.10146

Vitamin C is an important water-soluble vitamin that is needed by every living thing. Domestic animals, including ruminants, were capable of synthesizing ascorbic acid, primarily in the liver, therefore, vitamin C is not considered to be an essential nutrient for healthy ruminants. Nevertheless, in ruminants, vitamin C is important in biochemical reactions involving collagen cross-linking, hydroxylation of proline and lysine, and steroid and bile acid synthesis. However, in fact, Dietary vitamin C is extensively degraded in the rumen. Although ruminants are able to synthesize vitamin C, when ruminants experience stress and suffer from various diseases, vitamin C supplementation from outside is still needed to restore their health condition. Therefore, vitamin C supplements still have great benefits for optimizing their health conditions. This review discusses the effects of vitamin C and its supplementation on the health of ruminants.

Levels of Blood Glucose and Total Protein of Repeat Breeding Dairy Cows From Daerah Istimewa Yogyakarta Dhasia Ramandani; Alfarisa Nururrozi
Jurnal Sain Veteriner Vol 33, No 1 (2015): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Repeat breeding in dairy cows is a reproductive disorder characterized by a length of calving interval.This research was aimed to determine levels of blood glucose and total protein as one of the causes of repeat breeding. This research used ten repeat breeding Friesian Holstein cross breeds at the age of 3-8 years old from Daerah Istimewa Yogyakarta. They have had birth at least once, normal reproductive cycle, and in a healthy condition. Ten ml blood samples of each cow from jugular vein were collected and analyzed at LPPT UGM. Blood glucose and total protein plasm were examined using a photometer Microlab 300 with a spectrophotometer. The data ware analyzed descriptively. The results showed that dairy cows with repeatedbreeding case had lower blood glucose and total protein plasm concentrations than that of the normal. The average concentrations of blood glucose and total protein plasm were 48.58±6.675 mg/dl and 6.815±821 g/dl, respectively.

Terapi Ammonium Khlorida-Asam Askorbat untuk Menurunkan Tingkat Keasaman Urin dan Kristalisasi Struvit pada Kucing Urolithiasis (THERAPY AMMONIUM CHLORIDE-ASCORBIC ACID FOR LOWERING URINE ACIDITY AND STRUVITE CRYSTALLIZATION IN FELINE UROLITHIASIS) Alfarisa Nururrozi; Soedarmanto Indarjulianto; Yanuartono Yanuartono; Hary Purnamaningsih; Sitarina Widyarini; Slamet Raharjo; Dhasia Ramandani
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Struvite/Magnesium ammonium phosphate (MAP) is common causes of feline urolithiasis. Prevent the formation of MAP crystallization can be treated by adjusting the pH urine in acid conditions. Urine with an acid pH will decrease struvite activity product (SAP) by preventing the phosphate deprotonation process which is the main constituent of struvite components. This study aims to determine the potential of a combination of ammonium chloride (NH4Cl) and ascorbic acid to decrease SAP by retain the urine pH under normal conditions. Twelve male cats, 2-5 years old and body weight 3.0 ± 0.8 kg were diagnosed struvite urolithiasis used for this study. Cats have a9clinical history of hematuria, dysuria, polyuria, and stranguria. Diagnosis of urolithiasis performed by clinical examination, USG, x-rays, and urinalysis. Cats that found struvite urolith, treated with 200 mg/kg NH4Cl and 100 mg/kg ascorbic acid orally twice a day. The research objects were observed included urine pH and crystaluria density. The data were analyzed using analisis of varian. The result of this study showed NH4Cl and ascorbic acid treatment can decrease the urine pH at 8 cats (89%) with an average pH 6.3 ± 0.3. Microscopic examination of the urine showed the struvite crystallization more infrequently than before therapy. The study concluded that the combination of NH4Cl and asam askorbat can lower the urine pH and reduce struvite crystal density in the urine.

Profil Vulva dan Suhu Tubuh Kambing Peranakan Etawa pada Sinkronisasi Estrus Menggunakan Medroxy Progesterone Acetate dan Suplementasi Zinc (Zn) Muhammad Rosyid Ridlo; Risa Ummami; Naela Wanda Yusria Dalimunthe; Dhasia Ramandani; Nur Ika Prihanani; Morsid Andityas; Tri Satya Mastuti Widi
Jurnal Nasional Teknologi Terapan (JNTT) Vol 2, No 2 (2018): JULI
Publisher : Penelitian dan Pengabdian Kepada Masyarakat Sekolah Vokasi Universitas Gadjah Mada

Peranakan Etawa (PE) goat farming is one of the growing livestock sectors in Indonesia. Indonesia needs a sustainable breeding system that can guarantee the availability of goats. Estrus synchronization is a necessity that can help uniform the breeding time and manage the time of pregnancy and birth. Artificial estrus synchronization techniques that use a combination of Prosterone and Zinc minerals give good results of estrus synchronization in goats. The Aim of this research is to understand the combination efectiveness of estrus synchronization technique with progesterone acetate and Zinc supplementation. The design of this study used two treatment groups, each group consisting of three does PE goats. Estrus synchronization of experimental animals in treatment 1 and 2 was carried out using polyurethane sponge implant method which contained medroxi progesterone acetate 60 mg. Group 1 was given Sponge Progesterone for nine 9 days while giving Zinc (Zn) mineral supplement (Elemental Zn 20 mg, Kimia Farma) orally given 7 times. The frequency of Zinc administration every day begins during intra vaginal sponge insertion. Group 2 was treated same as group 1 with 14 days of implant time and 7 times of Zinc supplementation.The results showed that both treatments occurred estrus simultaneously with good estrus quality. Both treatments show the results of the estrus 8 score assessment with the estrus 3-9 range. The maximum estrus quality’s value of group 2 was higher (8.33 ± 0.57) compared to group 1 (8.0 ± 1.0). The estrus duration of group 2 occurs for 24 hours and group 1 for 7 hours. Estrus onset of Group 2 is earlier (58 hours) than group 1 (75 hours). Both treatment groups showed an increase in temperature at period of the occurrence of estrus. Body temperature during estrus time was 38.7 ± 0.20 0C to 39.60 ± 0.26 0C. The conclusion of this study is that in both treatments of estrus synchronization using sponge progesterone and zinc supplementation can cause estrus synchronization effectively . The duration of estrus in group 1 (9 days of implant) was Shorter than group 2 (14 days of implant). The onset of estrus time of group 1 (9 days of implant) was longer than Group 2 (14 days of implant). The body temperature of the experimental animals increases with the occurrence of estrus.

Potensi Bentonite Clay Dan Karbon Aktif Sebagai Aflatoksin Binders Berdasarkan Gambaran Imunologis Dan Histopatologis Pada Ayam Buras: Effects of Bentonite Clay and Activated Charcoal as Aflatoxin Binders based on the Immunological and Histopathological Changes in Kampung Chicken Dhasia Ramandani; Risa Ummami; Nurulia Hidayah; Naela Wanda Yusria Dalimunthe; Soedarmanto Indarjulianto; Yanuartono Yanuartono; Alfarisa Nururrozi
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol. 10 No. 1 (2020): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Ve
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/jipvet.v10i1.94

Aflatoxin is a toxic compound from the fungus A. flavus which can cause a decrease in chicken productivity. This study aims to evaluate the effect of bentonite clay and activated charcoal in chicken feed as aflatoxin binders based on histopathological and immunological features in chickens. A total of 60 chickens were randomly divided into 4 groups (G0, G1, G2, and G3) each group consisted of 15 chickens. G0 as a control without the addition of aflatoxin to the feed. Groups G1, G2, and G3â€™s feed were added with 106 ppb of aflatoxin. G2 was treated with 5g/kg bentonite clay, G3 was treated with 5g/kg activated charcoal. Chickens were vaccinated with Newcastle Disease (ND) on day 5. On days 14 and 28 serum was collected from the specimens for titer antibody testing. At the end of the study the chickens were dissected, the liver was taken for a histopathological test. The results of the aflatoxin level in the G0, G1, G2, and G3 groups were 10.5, 106, 19.1, and 23.95 ppb, respectively. Microscopic changes show the negative effects of aflatoxin on the liver organ. Group G0 did not show any histopathological changes, Group G2 and G3 showed several mild histopathological changes, whereas group G1 was the most severe. The antibody titers against Newcastle Disease (ND) of 4-weeks old chickens in G0, G1, G2, and G3 were 64, 4, 32, 32 HI units, respectively. The G0 treatment group has a protective titer against ND disease. Group G1 has the lowest (non-protective) ND antibody titer. Whereas the G2 and G3 groups both have protective titers against ND disease. Based on this study it was concluded that the administration of bentonite clay and activated charcoal was able to produce better immunological and histopathological features than the control group.

Uji Kualitas dan Uji Cemaran Daging Babi Pada Daging Sapi di Beberapa Pasar Tradisional di Yogyakarta: Pork Detection Test And Meat Physical Quality In Some Traditional Markets From Yogyakarta Risa Ummami; Dhasia Ramandani; Claude Mona Airin; Amir Husni; Pudji Astuti
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol. 12 No. 2 (2022): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Ve
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/jipvet.v12i2.277

Abstract Halal food is an essential thing to be considered by the Muslim community in Indonesia. Halal food must be free from pork, both as a primary ingredient and in the manufacturing process. Halal problems arise when there is a process of mixing meat or lard (adulteration) in halal animal meat for economic purposes. This study aims to identify the authenticity of beef circulating in several traditional markets in Yogyakarta using the Rapid Pork Detection Test and Enzyme-Linked Immunosorbent Assay (ELISA) detection methods. There were 10 samples of beef from several traditional markets in Yogyakarta. The tests carried out were organoleptic tests, Rapid Tests, and ELISA tests. The Rapid Pork Detection Test used is a special Rapid Test for detection of Xema® brand pork produced by PT. Indo Techno Plus. Sampling was carried out from May to June, the markets visited for sampling were the Pingit, Kranggan, Demangan, Ngasem, Godean, Sleman, Gamping, Mangiran and Sentul markets. The organoleptic examination includes odor, color, consistency, and pH. The results of the physical quality test of the meat showed that the beef circulating in the ten markets had a distinctive beef smell, red and pale red, had a chewy consistency that did not break down easily, and had a pH of 5 to 6 except for one sample (P9) from Sentul Market. The results of the Rapid Pork Detection Test showed a negative (-) pork content for all the beef samples. Meanwhile, in the ELISA test, there were three samples with positive results containing pork. The quality of beef circulating in these markets is within the normal range of meat quality and is safe for consumption. Keywords: Pork adulteration; Rapid Pork Detection; ELISA; meat physical quality. Abstrak Makanan halal merupakan suatu hal yang sangat penting untuk diperhatikan oleh masyarakat muslim di Indonesia. Makanan halal harus terbebas dari kandungan babi baik sebagai bahan dasar maupun dalam proses pembuatannya. Permasalahan kehalalan timbul ketika terdapat proses pencampuran daging atau lemak babi (adulterasi) pada daging hewan halal untuk tujuan ekonomis. Penelitian ini bertujuan untuk mengidentifikasi keaslian daging sapi yang beredar di beberapa pasar tradisional di Yogyakarta menggunakan metode deteksi Rapid Pork Detection Test dan Enzyme Linked Immunosorbent Assay (ELISA). Sampel berjumlah sebanyak 10 sampel daging sapi dari beberapa pasar tradisional di Yogyakarta. Uji yang dilakuan adalah uji organoleptik, uji Rapid Test dan uji ELISA. Rapid Pork Detection Test yang digunakan adalah Rapid Test khusus deteksi daging babi merk Xema® produksi PT. Indo Tekhno Plus. Sampling dilakukan pada bulan Mei sampai dengan Juni, pasar yang di kunjungi untuk pengambilan sampel adalah pasar Pingit, Kranggan, Demangan, Ngasem, Godean, Sleman, Gamping, Mangiran dan Sentul. Pemeriksaan organoleptik meliputi bau, warna, konsistensi dan pH. Hasil uji kualitas fisik daging menunjukkan bahwa daging sapi yang beredar di sepuluh pasar tersebut memiliki bau khas daging sapi, berwarna merah dan merah pucat, memiliki konsistensi kenyal tidak mudah terurai, dan memiliki pH 5 sampai dengan 6 kecuali satu sampel (P9) dari Pasar Sentul. Hasil uji Rapid Pork Detection Test menunjukkan hasil negatif (-) kandungan daging babi untuk semua sampel daging sapi tersebut. Sedangkan pada uji ELISA terdapat satu sampel dengan hasil positif mengandung babi. Kualitas daging sapi yang beredar di pasar-pasar tersebut masih dalam kisaran kualitas daging normal dan aman untuk dikonsumsi. Uji cemaran daging babi dapat dilakukan dengan menggunakan metode ELISA karena memiliki tingkat sensitivitas yang lebih tinggi dibandingkan dengan pengujian rapid test Kata kunci: Campuran babi; Rapid Pork Detection; ELISA; Kualitas fisik daging.

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