Garuda - Garba Rujukan Digital

INDUKSI MUTASI DAN SELEKSI IN VITRO TANAMAN GANDUM (Triticum aestivum L.) Sari, Laela; Purwito, Agus; Soepandi, Didy; Purnamaningsih, Ragapadmi; Sudarmonowati, Enny
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 3, No 2 (2016): December 2016
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

INDUCTION MUTATION AND SELECTION OF IN VITRO PLANT OF WHEAT (Triticum aestivumÂ L.)The goal of this research was to produce wheat crop which is tolerant to lowland condition.Six varieties were used, Dewata, Selayar, Alibey, Oasis, Rabe and HP1744. This research consisted of 4 stages: production of the best callus on MS medium containing 3 g/L 2.4-D, induced mutation of embryogenic callus using EMS, in vitro selection of callus at temperature of 27â35Â°C, and callus regeneration. The best result for callus production was 76% for Dewata and 70% for Selayar varieties. Higher concentration of EMS and longer soaking time decreased the percentage of callus growth. LC50 for Dewata was 0.3% EMS at 30 minutes and that for Selayar was 0.1% EMS at 60 minutes. The higher the temperature was, the lower was the adaptation tolerant of the plants, and callus growth was inhibited. At the highest temperature (35Â°C) the callus did not grow at all.Keywords: Induced mutation, Triticum aestivum, EMS, in vitro selection, callusABSTRAKTujuan penelitian ini adalah untuk merakit tanaman gandum yang toleran pada dataran rendah. Varietas yang digunakan ada 6 yaitu Dewata, Selayar, Alibey, Oasis, Rabe dan HP-1744. Penelitian terdiri atas empat tahap yaitu induksi pembentukan kalus terbaik menggunakan media MS + 3 g/L 2,4-D (dipilih dua varietas yang terbaik), induksi mutasi kalus embriogenik menggunakan EMS, seleksi kalus in vitro pada suhu 27â35Â°C, dan regenerasi. Hasil induksi kalus terbaik terdapat pada varietas Dewata sebesar 76% dan Selayar sebesar 70%. Semakin tinggi konsentrasi EMS dan semakin lama waktu perendaman yang digunakan maka semakin menurun persentase pertumbuhan kalus. LC50 varietas Dewata adalah EMS 0,3% waktu 30 menit sedangkan LC50 varietas Selayar adalah EMS 0,1% waktu 60 menit.Semakin tinggi suhunya maka semakin berkurang toleran adaptasi tanaman tersebut, dan pertumbuhan kalus semakin sedikit. Bahkan pada suhu tertinggi yaitu suhu 35Â°C tidak ada pertumbuhan kalus sama sekali.Kata Kunci: Induksi mutasi, Triticum aestivum, EMS, seleksi in vitro, kalus

Agronomic Characterization of Wheat Mutants (Triticum aestivum) of M3 Generation Planted in Sukabumi Sari, Laela; Purwito, Agus; Sopandie, Didy; Purnamaningsih, Ragapadmi; Sudarmonowati, Enny
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6612

The purpose of this study was to identify the selection criteria to obtain a superior mutant derived from the wheat plants of such varieties as Dewata, Selayar and Alibey, adaptive in medium land. The analysis of agronomic growth characters showed a significantly effect on a growth percentage of the initial growth (8 mutants), flowering time (1 mutant), panicle stem length (15 mutants), number of panicles (7 mutants), the number of grains per panicle (8 mutants), grain weight observed (8 mutants), grain weight per genotype (6 mutants), leaf area (2 mutants) and leaf greenness (5 mutants). The effects on the characters of ripe time, harvest, panicle length and plant height were not significant. The mutants of Dewata, Selayar and Alibey could be selected based on the characters of panicle stem length, number of grains per panicle and grain weight per observation because these characters generated more mutants than the other characters. The correlation analysis between the characters of growth and yield components of wheat mutants showed that the number of grains per panicle was positively correlated with the grain weight observed, while the length of panicle stem was positively correlated with grain weight per genotype, number of panicles and leaf area. Hopefully some mutants produced could adapt to the tropical medium land, thus adding to the diversity of wheat germplasm in Indonesia, thereby reducing the import of wheat to Indonesia.How to CiteSari, L., Purwito, A., Sopandie, D., Purnamaningsih, R. & Sudarmonowati, E. (2016). Agronomic Characterization of Wheat Mutants (Triticum aestivum) of M3 Generation Planted in Sukabumi. Biosaintifika: Journal of Biology & Biology Education, 8(3), 353-361.

Propagation of Sukun (Artocarpus altilis (Parkinson) Fosberg) through In Vitro Shoot Proliferation Imelda, Maria; Wulansari, Aida; Sari, Laela
ANNALES BOGORIENSES Vol 13, No 1 (2009): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/28

Sukun (Artocarpus altilis (Parkinson) Fosberg) of the Moraceaae is a big tree which can grow to 15-20 m in height and native toÂ the Asia-Pasific region.Â Beside its delicious fruit, sukun is also known as aÂ traditional herbal medicinal plant in the region, including Indonesia.Â Nearly all parts of theÂ plant, such as roots, stems and leaves are believed by local communities to be capable of curing liver disease, hypertension, cardiac arrest, toothache, renal problem and even skin itchiness. The collaborative research between LIPI and PR China, on developing herbal medicines indicated that sukun has a great potential for treating cardiovascular disease.Â However, the availability of raw materials still poses a big constraint for the industry of herbal medicines. Generally, sukunÂ is propagated by root or stem cuttings, since in IndonesiaÂ sukun does not produce any seeds. However suchÂ method only produces limited planting materials. In general tissue culture propagated plants have many advantages, namely being clonal,Â Â free from pest and diseases, more uniform,Â andÂ allowing a high rate ofÂ plant multiplication. Therefore, the technique for sukun propagation has been developed by the LIPI Research Centre for Biotechnology. In this research the effects ofÂ 1-5 mg/l benzyl amino purine (BAP) and 20-40 mg/l adenine sulphate (AS) on shoot bud proliferation were investigated using lateral shoot buds on a modified Murashige and Skoog (MS) medium with addition of 150 ml/l coconut water(CW). Shoots were rooted on MS medium without plant growth regulators (PGRs). The results showed that the best medium forÂ in vitro shoot proliferation was a modified MS medium containing 2 mg/l BAP, 40 mg/l AS and 150 ml/l CW. The best medium for rooting is MS mediumÂ containing 1 mg indole butyric acid (IBA), producing roots within 3 weeks.Â Keywords : Sukun (Artocarpus altilis),Â in vitro shoot proliferation, Benzyl amino purine (BAP), Adenine sulphate (AS), coconut water (CW), Indole butyric acid (IBA)

PERBANYAKAN IN VITRO PISANG KEPOK var. UNTI SAYANG TAHAN PENYAKIT DARAH MELALUI PROLIFERASI TUNAS Imelda, Maria; Wulansari, Aida; Sari, Laela
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 5, No 1 (2018): June 2018
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

In Vitro Propagation of Kepok Banana var. Unti Sayang Resistant to Blood Disease through Shoot ProliferationABSTRACTKepok is a popular banana variety but sensitive to blood disease caused by Ralstonia solanacearum (Smith). The discovery of a natural mutant of Kepok banana var. Unti Sayang from Sulawesi which male bud falls naturally, is a shortcut to bypass the chains of the spread of blood disease, since the disease is transmitted by insects through the wounds of the male buds. The superior mutant needs to be mass propagated and disseminated to endemic areas to inhibit the spread of blood disease. To achieve that goal, an efficient and effective techniques of in vitro shoot proliferation needs to be developed. Shoot proliferation was performed by addition of BAP, thidiazuron and adenine sulphate. The results showed that the best medium for shoot multiplication was B2T5A (MS+2 mg/L BAP+0,5 mg/L TDZ+20 mg/L adenine sulphate), and for shoot growth was B4A (MS+4 mg/L BAP+20 mg/L adenine sulphate). Rooting was induced on MS medium without hormones. Acclimatization of plantlets on mixed soil, compost and husks with a ratio of 1:1:1 resulted in 92,35% survival rate.Keywords: blood disease, in vitro shoot, Â male budless, natural mutant, var. Unti Sayang Â ABSTRAKPisang kepok merupakan varietas yang digemari tetapi sangat peka terhadap penyakit darah yang ditimbulkan oleh bakteri Ralstonia solanacearum (Smith). Ditemukannya mutan alami pisang kepok yang jantungnya gugur secara alami yaitu varietas Unti Sayang dari Sulawesi, merupakan jalan pintas untuk memotong rantai penyebaran penyakit darah, mengingat penyakit ini ditularkan oleh serangga melalui luka bekas bunga jantan pada jantung. Mutan unggul tersebut perlu diperbanyak secara massal dan disebarluaskan ke daerah endemik untuk menghambat penyebaran penyakit darah. Untuk mencapai tujuan tersebut, perlu dikembangkan teknik perbanyakan in vitro pisang kepok Unti Sayang yang efektif dan efisien melalui proliferasi tunas. Proliferasi tunas dilakukan dengan penambahan BAP, thidiazuron dan adenin sulfat. Hasil penelitian ini menunjukkan bahwa media terbaik untuk multiplikasi tunas adalah B2T5A (MS+2 mg/L BAP+0,5 mg/L TDZ+20 mg/L adenin sulfat), media terbaik untuk pertumbuhan tunas adalah B4A (MS+4 mg/L BAP+20 mg/L adenin sulfat). Akar dapat diinduksi pada media MS tanpa hormon. Aklimatisasi planlet pada media campuran tanah, kompos dan sekam dengan perbandingan 1:1:1 menghasilkan 92,35% planlet hidup.Kata Kunci: penyakit darah, tunas in vitro, tanpa jantung, mutan alami, var. Unti SayangÂ

MIKROPROPAGASI TANAMAN TALAS BENENG (Xanthosoma undipes K. Koch) DENGAN PERLAKUAN BENZIL AMINOPURIN, TIAMIN, DAN ADENIN Sari, Laela; Wulansari, Aida; Noorrohmah, Siti; Ermayanti, Tri Muji
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 6, No 1 (2019): June 2019
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v6i1.3216

Micropropagation of Beneng Taro (Xanthosoma undipes K. Koch) with Benzyl Amino Purine, Thiamine, and Adenine TreatmentABSTRACTConventional production of Beneng taro seeds (Xanthosoma undipes K. Koch) is constrained by the limited number of tubers, thus an alternative solution is needed such as in vitro propagation. This study was aimed to obtain a micropropagation technique of Beneng taro on MS media with BAP, thiamine, and adenine treatment, and to determine its growth at the acclimatization stage. This research consisted of shoot multiplication and acclimatization. Shoot propagation was carried out on MS media with 8 treatments, namely ½MS and MS without addition of growth promoting substance, and MS with 1, 2 and 3 mg×L-1 BAP, with or without addition of 1 mg×L-1 thiamine and 2 mg×L-1 adenine. Each treatment was replicated four times, each consisting of four shoots. Growth observation was made from 1st to 5th week on petiole length, and number of shoots, leaves and roots. Acclimatization was carried out on soil media, compost, and husks in a ratio of 1: 1: 1. The results showed that the best media for shoot multiplication was MS + 1 mg×L-1 BAP + 1 mg×L-1 thiamine + 2 mg×L-1 adenine with an average of 3.5 shoots, while the best medium for the petiole length was ½MS with an average value of 6.97 cm. The results of acclimatization showed that 100% planlets survived, and plantlets grown on MS media + 3 mg×L-1 BAP had the highest number of shoots with an average of 4.2.Keywords: adenine, Beneng taro, benzil amino purine (BAP), micropropagation, thiamineABSTRAKPenyediaan bibit talas Beneng (Xanthosoma undipes K. Koch) secara konvensional terkendala terbatasnya jumlah umbi, sehingga perlu solusi alternatif, diantaranya melalui perbanyakan in vitro. Tujuan penelitian ini adalah untuk mendapatkan teknik mikropropagasi talas beneng pada media MS dengan perlakuan BAP, tiamin, adenin, dan untuk mengetahui pertumbuhannya pada tahap aklimatisasi. Penelitian ini meliputi perbanyakan tunas dan aklimatisasi. Perbanyakan tunas menggunakan media MS dengan 8 perlakuan yaitu ½MS dan MS tanpa penambahan zat pengatur tumbuh (ZPT), serta MS dengan 1, 2 dan 3 mg×L-1 BAP dengan atau tanpa penambahkan 1 mg×L-1 tiamin dan 2 mg×L-1 adenin. Setiap perlakuan mempunyai empat ulangan, setiap ulangan terdiri atas empat tunas. Pertumbuhan diamati mulai minggu ke-1 hingga ke-5 terhadap panjang petiol serta jumlah anakan, daun dan akar. Aklimatisasi dilakukan pada media tanah, kompos dan sekam dengan perbandingan 1:1:1. Hasil menunjukkan bahwa media terbaik perbanyakan tunas adalah MS + 1 mg×L-1 BAP + 1 mg×L-1 tiamin + 2 mg×L-1 adenin dengan rata-rata 3,5 tunas, sedangkan media terbaik untuk panjang tangkai daun adalah ½MS dengan nilai rata-rata 6,97 cm. Hasil aklimatisasi menunjukkan bahwa 100% planlet hidup dan planlet yang ditumbuhkan pada media MS + 3 mg×L-1 BAP mempunyai jumlah anakan terbanyak dengan rata-rata 4,2.Kata Kunci: adenine, benzil amino purin (BAP), mikropropagasi, talas Beneng, tiamine

PUSAT PENELITIAN BIOTEKNOLOGI-LIPI, JL. RAYA BOGOR KM. 46, CIBINONG, 16911, JAWA BARAT, INDONESIA Susetio, Muhammad; Efendi, Darda; Sari, Laela
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3761

ABSTRACTBentul taro (Colocasia esculenta) is one of taro cultivar which has high productivity. Genetic improvement has been done by obtaining tetraploid taro. Drought tolerant cultivars can be achieved by addition of poly ethylene glycol (PEG) in vitro. The purpose of this study was to determine the effect of the treatment of PEG concentrations on in vitro growth of tetraploid shoots and to propagate shoots after PEG treatment. The explants used were diploid taro shoots, tetraploid clones 2.1.2 and 2.4.2. Shoots were planted in MS media without PEG and with PEG of 5, 10 and 15%. The growth parameters observed were petiole length, number of leaves, and number of roots every week for 6 weeks. Proline content and LC50 values were analyzed at 6 weeks after planting. Propagation of shoots after PEG treatment was carried out on MS media containing 2 mg / L BAP, 1 mg / L thiamine, and 2 mg / L adenine. Each treatment consisted of 15 shoots as replicates. The growth parameters observed were petiole length, number of leaves, and number of shoots every week for 6 weeks. The results showed that Bentul taro clones significantly affected the concentration of PEG on growth parameters. LC50 value of diploid clone was 12.16%, clone 2.1.2 was 13.54%, and clone 2.4.2 was 12.74%. The highest proline content was found at Bentul tetraploid clone 2.1.2. After PEG treatment, growth was significantly affected by PEG concentrations. All Bentul taro clones after PEG treatment produced multiple shoots. Keywords: Taro (Colocasia esculenta), in vitro selection, diploid, tetraploid, proline, LC50, propagation

PUSAT PENELITIAN BIOTEKNOLOGI-LIPI, JL. RAYA BOGOR KM. 46, CIBINONG, 16911, JAWA BARAT, INDONESIA Susetio, Muhammad; Efendi, Darda; Sari, Laela
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3761

ABSTRACTBentul taro (Colocasia esculenta) is one of taro cultivar which has high productivity. Genetic improvement has been done by obtaining tetraploid taro. Drought tolerant cultivars can be achieved by addition of poly ethylene glycol (PEG) in vitro. The purpose of this study was to determine the effect of the treatment of PEG concentrations on in vitro growth of tetraploid shoots and to propagate shoots after PEG treatment. The explants used were diploid taro shoots, tetraploid clones 2.1.2 and 2.4.2. Shoots were planted in MS media without PEG and with PEG of 5, 10 and 15%. The growth parameters observed were petiole length, number of leaves, and number of roots every week for 6 weeks. Proline content and LC50 values were analyzed at 6 weeks after planting. Propagation of shoots after PEG treatment was carried out on MS media containing 2 mg / L BAP, 1 mg / L thiamine, and 2 mg / L adenine. Each treatment consisted of 15 shoots as replicates. The growth parameters observed were petiole length, number of leaves, and number of shoots every week for 6 weeks. The results showed that Bentul taro clones significantly affected the concentration of PEG on growth parameters. LC50 value of diploid clone was 12.16%, clone 2.1.2 was 13.54%, and clone 2.4.2 was 12.74%. The highest proline content was found at Bentul tetraploid clone 2.1.2. After PEG treatment, growth was significantly affected by PEG concentrations. All Bentul taro clones after PEG treatment produced multiple shoots. Keywords: Taro (Colocasia esculenta), in vitro selection, diploid, tetraploid, proline, LC50, propagation

MIKROPROPAGASI TANAMAN TALAS BENENG (Xanthosoma undipes K. Koch) DENGAN PERLAKUAN BENZIL AMINOPURIN, TIAMIN, DAN ADENIN Sari, Laela; Wulansari, Aida; Noorrohmah, Siti; Ermayanti, Tri Muji
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 1 (2019): June 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v6i1.3216

Micropropagation of Beneng Taro (Xanthosoma undipes K. Koch) with Benzyl Amino Purine, Thiamine, and Adenine TreatmentABSTRACTConventional production of Beneng taro seeds (Xanthosoma undipes K. Koch) is constrained by the limited number of tubers, thus an alternative solution is needed such as in vitro propagation. This study was aimed to obtain a micropropagation technique of Beneng taro on MS media with BAP, thiamine, and adenine treatment, and to determine its growth at the acclimatization stage. This research consisted of shoot multiplication and acclimatization. Shoot propagation was carried out on MS media with 8 treatments, namely Ã‚Â½MS and MS without addition of growth promoting substance, and MS with 1, 2 and 3 mgÃƒâ€”L-1 BAP, with or without addition of 1 mgÃƒâ€”L-1 thiamine and 2 mgÃƒâ€”L-1 adenine. Each treatment was replicated four times, each consisting of four shoots. Growth observation was made from 1st to 5th week on petiole length, and number of shoots, leaves and roots. Acclimatization was carried out on soil media, compost, and husks in a ratio of 1: 1: 1. The results showed that the best media for shoot multiplication was MS + 1 mgÃƒâ€”L-1 BAP + 1 mgÃƒâ€”L-1 thiamine + 2 mgÃƒâ€”L-1 adenine with an average of 3.5 shoots, while the best medium for the petiole length was Ã‚Â½MS with an average value of 6.97 cm. The results of acclimatization showed that 100% planlets survived, and plantlets grown on MS media + 3 mgÃƒâ€”L-1 BAP had the highest number of shoots with an average of 4.2.Keywords: adenine, Beneng taro, benzil amino purine (BAP), micropropagation, thiamineABSTRAKPenyediaan bibit talas Beneng (Xanthosoma undipes K. Koch) secara konvensional terkendala terbatasnya jumlah umbi, sehingga perlu solusi alternatif, diantaranya melalui perbanyakan in vitro. Tujuan penelitian ini adalah untuk mendapatkan teknik mikropropagasi talas beneng pada media MS dengan perlakuan BAP, tiamin, adenin, dan untuk mengetahui pertumbuhannya pada tahap aklimatisasi. Penelitian ini meliputi perbanyakan tunas dan aklimatisasi. Perbanyakan tunas menggunakan media MS dengan 8 perlakuan yaitu Ã‚Â½MS dan MS tanpa penambahan zat pengatur tumbuh (ZPT), serta MS dengan 1, 2 dan 3 mgÃƒâ€”L-1 BAP dengan atau tanpa penambahkan 1 mgÃƒâ€”L-1 tiamin dan 2 mgÃƒâ€”L-1 adenin. Setiap perlakuan mempunyai empat ulangan, setiap ulangan terdiri atas empat tunas. Pertumbuhan diamati mulai minggu ke-1 hingga ke-5 terhadap panjang petiol serta jumlah anakan, daun dan akar. Aklimatisasi dilakukan pada media tanah, kompos dan sekam dengan perbandingan 1:1:1. Hasil menunjukkan bahwa media terbaik perbanyakan tunas adalah MS + 1 mgÃƒâ€”L-1 BAP + 1 mgÃƒâ€”L-1 tiamin + 2 mgÃƒâ€”L-1 adenin dengan rata-rata 3,5 tunas, sedangkan media terbaik untuk panjang tangkai daun adalah Ã‚Â½MS dengan nilai rata-rata 6,97 cm. Hasil aklimatisasi menunjukkan bahwa 100% planlet hidup dan planlet yang ditumbuhkan pada media MS + 3 mgÃƒâ€”L-1 BAP mempunyai jumlah anakan terbanyak dengan rata-rata 4,2.Kata Kunci: adenine, benzil amino purin (BAP), mikropropagasi, talas Beneng, tiamine

PERBANYAKAN IN VITRO PISANG KEPOK var. UNTI SAYANG TAHAN PENYAKIT DARAH MELALUI PROLIFERASI TUNAS Imelda, Maria; Wulansari, Aida; Sari, Laela
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 1 (2018): June 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

In Vitro Propagation of Kepok Banana var. Unti Sayang Resistant to Blood Disease through Shoot ProliferationABSTRACTKepok is a popular banana variety but sensitive to blood disease caused by Ralstonia solanacearum (Smith). The discovery of a natural mutant of Kepok banana var. Unti Sayang from Sulawesi which male bud falls naturally, is a shortcut to bypass the chains of the spread of blood disease, since the disease is transmitted by insects through the wounds of the male buds. The superior mutant needs to be mass propagated and disseminated to endemic areas to inhibit the spread of blood disease. To achieve that goal, an efficient and effective techniques of in vitro shoot proliferation needs to be developed. Shoot proliferation was performed by addition of BAP, thidiazuron and adenine sulphate. The results showed that the best medium for shoot multiplication was B2T5A (MS+2 mg/L BAP+0,5 mg/L TDZ+20 mg/L adenine sulphate), and for shoot growth was B4A (MS+4 mg/L BAP+20 mg/L adenine sulphate). Rooting was induced on MS medium without hormones. Acclimatization of plantlets on mixed soil, compost and husks with a ratio of 1:1:1 resulted in 92,35% survival rate.Keywords: blood disease, in vitro shoot, Ã‚Â male budless, natural mutant, var. Unti Sayang Ã‚Â ABSTRAKPisang kepok merupakan varietas yang digemari tetapi sangat peka terhadap penyakit darah yang ditimbulkan oleh bakteri Ralstonia solanacearum (Smith). Ditemukannya mutan alami pisang kepok yang jantungnya gugur secara alami yaitu varietas Unti Sayang dari Sulawesi, merupakan jalan pintas untuk memotong rantai penyebaran penyakit darah, mengingat penyakit ini ditularkan oleh serangga melalui luka bekas bunga jantan pada jantung. Mutan unggul tersebut perlu diperbanyak secara massal dan disebarluaskan ke daerah endemik untuk menghambat penyebaran penyakit darah. Untuk mencapai tujuan tersebut, perlu dikembangkan teknik perbanyakan in vitro pisang kepok Unti Sayang yang efektif dan efisien melalui proliferasi tunas. Proliferasi tunas dilakukan dengan penambahan BAP, thidiazuron dan adenin sulfat. Hasil penelitian ini menunjukkan bahwa media terbaik untuk multiplikasi tunas adalah B2T5A (MS+2 mg/L BAP+0,5 mg/L TDZ+20 mg/L adenin sulfat), media terbaik untuk pertumbuhan tunas adalah B4A (MS+4 mg/L BAP+20 mg/L adenin sulfat). Akar dapat diinduksi pada media MS tanpa hormon. Aklimatisasi planlet pada media campuran tanah, kompos dan sekam dengan perbandingan 1:1:1 menghasilkan 92,35% planlet hidup.Kata Kunci: penyakit darah, tunas in vitro, tanpa jantung, mutan alami, var. Unti SayangÃ‚Â

INDUKSI MUTASI DAN SELEKSI IN VITRO TANAMAN GANDUM (Triticum aestivum L.) Sari, Laela; Purwito, Agus; Soepandi, Didy; Purnamaningsih, Ragapadmi; Sudarmonowati, Enny
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 3 No. 2 (2016): December 2016
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

INDUCTION MUTATION AND SELECTION OF IN VITRO PLANT OF WHEAT (Triticum aestivumÃ‚Â L.)The goal of this research was to produce wheat crop which is tolerant to lowland condition.Six varieties were used, Dewata, Selayar, Alibey, Oasis, Rabe and HP1744. This research consisted of 4 stages: production of the best callus on MS medium containing 3 g/L 2.4-D, induced mutation of embryogenic callus using EMS, in vitro selection of callus at temperature of 27Ã¢â‚¬â€œ35Ã‚Â°C, and callus regeneration. The best result for callus production was 76% for Dewata and 70% for Selayar varieties. Higher concentration of EMS and longer soaking time decreased the percentage of callus growth. LC50 for Dewata was 0.3% EMS at 30 minutes and that for Selayar was 0.1% EMS at 60 minutes. The higher the temperature was, the lower was the adaptation tolerant of the plants, and callus growth was inhibited. At the highest temperature (35Ã‚Â°C) the callus did not grow at all.Keywords: Induced mutation, Triticum aestivum, EMS, in vitro selection, callusABSTRAKTujuan penelitian ini adalah untuk merakit tanaman gandum yang toleran pada dataran rendah. Varietas yang digunakan ada 6 yaitu Dewata, Selayar, Alibey, Oasis, Rabe dan HP-1744. Penelitian terdiri atas empat tahap yaitu induksi pembentukan kalus terbaik menggunakan media MS + 3 g/L 2,4-D (dipilih dua varietas yang terbaik), induksi mutasi kalus embriogenik menggunakan EMS, seleksi kalus in vitro pada suhu 27Ã¢â‚¬â€œ35Ã‚Â°C, dan regenerasi. Hasil induksi kalus terbaik terdapat pada varietas Dewata sebesar 76% dan Selayar sebesar 70%. Semakin tinggi konsentrasi EMS dan semakin lama waktu perendaman yang digunakan maka semakin menurun persentase pertumbuhan kalus. LC50 varietas Dewata adalah EMS 0,3% waktu 30 menit sedangkan LC50 varietas Selayar adalah EMS 0,1% waktu 60 menit.Semakin tinggi suhunya maka semakin berkurang toleran adaptasi tanaman tersebut, dan pertumbuhan kalus semakin sedikit. Bahkan pada suhu tertinggi yaitu suhu 35Ã‚Â°C tidak ada pertumbuhan kalus sama sekali.Kata Kunci: Induksi mutasi, Triticum aestivum, EMS, seleksi in vitro, kalus

Title

Found 10 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 10 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 10 Documents
Search