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All Journal Majalah Kedokteran Gigi Indonesia Jurnal Florea
Nunuk Purwanti, Nunuk
Bagian Biomedika, Fakultas Kedokteran Gigi, Universitas Gadjah Mada, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry

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Pengaruh Ekstrak Kulit Jeruk Nipis (Citrus Aurantifolia Swingle) Konsentrasi 10% Terhadap Aktivitas Enzim Glukosiltransferase Streptococcus mutans U, Zenia Adindaputri; Purwanti, Nunuk; Wahyudi, Ivan Arie
Majalah Kedokteran Gigi Indonesia Vol 20, No 2 (2013)
Publisher : Majalah Kedokteran Gigi Indonesia

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Abstract

Streptococcus mutans merupakan bakteri yang berperan sebagai agen utama penyebab karies gigi, yang memiliki enzim glukosiltransferase (GTF). Enzim GTF akan mengubah sukrosa menjadi fruktosa dan glukan. Salah satu herbal tradisional yang dapat berperan sebagai antibakteri adalah kulit jeruk nipis (Citrus aurantifolia Swingle) yang mengandung polifenol terutama flavonoid. Tujuan penelitian ini untuk mengetahui pengaruh ekstrak kulit jeruk nipis (Citrus aurantifolia Swingle) konsentrasi 10% terhadap aktivitas enzim GTF Streptococcus mutans. Penelitian ini menggunakan ekstrak kulit jeruk nipis konsentrasi 10% sebagai perlakuan, chlorhexidine gluconate 0,12% sebagai kontrol positif, serta akuades steril sebagai kontrol negatif. Metode penelitian ini terdiri dari tiga tahap yaitu penyiapan ekstrak kulit jeruk nipis konsentrasi 10%, penyiapan enzim GTF dari supernatan Streptococcus mutans, dan pengujian aktivitas enzim GTF melalui analisis konsentrasi fruktosa dengan menggunakan High Performance Liquid Chromatography (HPLC). Pembacaan luas area fruktosa dilakukan berdasarkan waktu retensi. Satu unit aktivitas enzim GTF di definisikan sebagai 1 µmol fruktosa/ml dari enzim/jam. Selanjutnya data yang diperoleh dianalisis secara statistik dengan one way ANOVA.Hasil perhitungan aktivitas enzim GTF dengan one way ANOVA menunjukkan perbedaan yang signifikan antara kelompok perlakuan dengan kelompok kontrol negatif (p<0,05), dan tidak terdapat perbedaan yang signifikan dengan kontrol positif. Kesimpulan dari penelitian ini adalah ekstrak kulit jeruk nipis konsentrasi 10% dapat menghambat aktivitas enzim glukosiltransferase Streptococcus mutans. ABSTRACT, The Influence of 10% Concentrate of Citrus Aurantifolia Swingle on The Activities of Streptococcus Mutans Glucocyl Transferase Enzyme. Streptococcus mutans is a bacteria which has glucosyl transferase (GTF) enzyme and acts as the main agent that causes dental caries. GTF enzyme will convert sucrose into fructose and glucan. Lime peel (Citrus aurantifolia Swingle) is one of the traditional herbs which has flavonoid as an antibacterial agent. The purpose of this research is to investigate the effect of 10% concentration of lime peel extract (Citrus aurantifolia Swingle) to the activity of GTF enzyme Streptococcus mutans.This research used 10% concentration of  lime peel extract as the treatment, 0.12% chlorhexidine gluconate as a positive control, and distillate water as anegative control. The method of this research consists of three steps; preparing the lime peel extract concentration of 10%, preparing the GTF enzyme from the supernatant of Streptococcus mutans, and testing GTF enzyme activity by analyzing the fructose concentration using High Performance Liquid Chromatography (HPLC). Perusal of the fructose area was based on the retention time of fructose. One unit of GTF enzyme activity is defined as the 1 μmol fructose / ml of enzyme / hour.  The obtained data then were analyzed by one way ANOVA. The result showed a significant difference between treatment group with the negative control (p <0.05), and there are no significant difference with the positive control. This research concludes that 10% lime peel extract can inhibit the GTF enzyme activity of Streptococcus mutans.
Infiltrasi Neutrofil pada Tikus dengan Periodontitis setelah Pemberian Ekstrak Etanolik Kulit Manggis Prasetya, Rendra Chriestedy; Purwanti, Nunuk; Haniastuti, Tetiana
Majalah Kedokteran Gigi Indonesia Vol 21, No 1 (2014)
Publisher : Majalah Kedokteran Gigi Indonesia

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Abstract

Periodontitis adalah inflamasi kronis yang disebabkan oleh bakteri periodontopatogen. Pada periodontitis terjadi peningkatan infiltrasi neutrofil yang berfungsi untuk memfagositosis bakteri yang menginfiltrasi jaringan gingiva. Kulit manggis mempunyai bahan aktif yaitu xanton yang mempunyai efek antiinflamasi dengan jalan menghambat sintesis PGE2 sehingga akan menurunkan infiltrasi sel inflamasi. Penelitian ini bertujuan untuk mengetahui infiltrasi sel neutrofil pada periodontitis setelah pemberian ekstrak etanolik kulit manggis. Tikus wistar jantan sebanyak empat puluh delapan ekor diinduksi periodontitis dengan ligasi pada daerah subgingiva gigi anterior rahang bawah selama 7 hari. Setelah hari ke-7, ligasi dilepas selanjutnya tikus dibagi menjadi 4 kelompok perlakuan yaitu ekstrak kulit manggis 30 mg/kg BB dan 60 mg/kg BB, ibuprofen dan saline. Tikus dikorbankan pada hari ke-1, 3, 5 dan 7 setelah perlakuan. Jaringan pada bagian anterior rahang bawah ditanam dalam paraffin dan dilakukan pemotongan serial kemudian diwarnai dengan hematoxylin eosin. Jumlah neutrofil dihitung di bawah mikroskop dengan perbesaran 400x. Data jumlah neutrofil dianalisa dengan Two Way Anova. Hasil Two Way Anova menunjukkan perbedaan yang bermakna rerata sel neutrofil antar kelompok perlakuan (p<0,05) mengindikasikan pemberian ekstrak etanolik kulit manggis berpengaruh terhadap jumlah sel neutrofil. Dapat disimpulkan bahwa pemberian ekstrak etanolik kulit manggis mampu menurunkan infiltrasi sel neutrofil pada tikus yang diinduksi periodontitis. ABSTRACT: Neutrophil infiltration in rats with periodontitis after the granting of Ethanolic Extract Skin Mangosteen. Periodontitis is a chronic inflammatory disease caused by periodontopathic bacteria. When periodontitis occurs are followed by neutrophil infiltration. Mangosteen rind contains xanthone, an anti-inflammatory substance which inhibits the synthesis of PGE2 and therefore reduces inflammatory cells infiltration. This research aimed to study neutrophil cells infiltration in experimental-induced periodontitis rats after mangosteen rind ethanolic extract administration. Forty-eight male wistar rats were induced the periodontitis by putting silk ligature subgingivally around the neck of the anterior lower teeth for seven days. After the ligation had been taken out, the rats were divided into four groups and treated orally with mangosteen rind extract 60 mg/kg BB, 30 mg/kg BB, ibuprofen, and saline. The rats were sacrificed on the 1st, 3rd, 4th, seventh day after the treatment. Their anterior lower jaws were processed for paraffin embedded tissue, cut serially and stained with hematoxylin-eosin. The neutrophil cells were observed and counted under the microscope (400x). The data were analyzed using Anova. Anova result showed a significant difference among group (p<0,05) indicating that mangosteen rind ethanolic extract affected the number of neutrophils. In conclusion, mangosteen rind ethanolic extract reduced the number of neutrophil infiltration in periodontitis rats.
PENINGKATAN MOTIVASI DAN PRESTASI BELAJAR BIOLOGI KELAS X-7 SMA N 1 BARAT MELALUI PENERAPAN MODEL THINK PAIR SHARE Purwanti, Nunuk
Jurnal Florea Vol 2, No 2 (2015)
Publisher : Jurnal Florea

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Abstract

Motivation plays an important role in learning, X-7 grade students of SMA Negeri 1 Barat shows theknown to be less motivated to follow the teaching of Biology. The aim of research to increase themotivation to learn biology X - 7 grade SMA Negeri 1 Barat with the application Think Pair Share(TPS). This type of research is classroom action research with planning, action, observation, andreflection, material monera and protists conducted in two cycles. Research subject class X-7 SMANegeri 1 Barat of academic year 2014/2015. The results showed cooperative learning Think Pair Sharecan help students overcome the problem for expression and enhance self-confidence of students, so asto increase the motivation of students.Keywords: motivation to learn, think pair share
Dealing with the high-risk potential of COVID-19 cross-infection in dental practice Yulianto, Heribertus Dedy Kusuma; Purwanti, Nunuk; Utami, Trianna Wahyu; Dewi, Anne Handrini; Listyarifah, Dyah; Ruspita, Intan; Nur, Asikin; Susilowati, Heni
Majalah Kedokteran Gigi Indonesia Vol 6, No 1 (2020): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.56588

Abstract

The World Health Organization reported that the SARS-CoV-2 virus has infected more than 5 million people around the world. Dental care providers and health care professionals need to be aware of the high-risk potential of crossinfection since the routes of virus transmission commonly happen through droplets and aerosols. This review aimed at collecting essential knowledge about the COVID-19 needed by dental practitioners. The review focused on the oral involvement in COVID-19, the role of oral transmission as the high-risk potential of cross-infection and recommended strategies to minimize the risk of cross-infection in dental practice. We searched all the published clinical features from PubMed, Google Scholar, Scopus and hand searched library online databases, from January 2015 until May 2020. Keywords used were “COVID-19”, “Dentistry”, “Dental protection”, “Cross-contamination”, “Aerosol and non aerosol”, and ”Povidone-iodine” with their combinations. We identified 52 articles to review after the initial selection with inclusion and exclusion criteria. Results showed use of topical applications of povidine-iodine and viricidal mouthwash could significantly reduce the high-risk of cross-infection from dentistry patients who are asymptomatic with COVID-19 infection. Further safeguards include suspending all non-emergency procedures temporarily and closely screening patients for symptoms which may be suspected to be COVID-19 infection.
Co-Authors Anne Handrini Dewi Asikin Nur Dyah Listyarifah, Dyah Heni Susilowati Heribertus Dedy Kusuma Yulianto Intan Ruspita, Intan Ivan Arie Wahyudi Rendra Chriestedy Prasetya Trianna Wahyu Utami Zenia Adindaputri U, Zenia Adindaputri