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Adulticide Efficacy of Artemisia vulgaris L. against Aedes aegypti L. Ninditya, Vika Ichsania; Purwati, Endah; Utami, Ajeng Tyas; Marwaningtyaz, Aprillyani Sofa; Fairuz, Nadia Khairunnisa; Hamid, Penny Humaidah
Jurnal ILMU DASAR Vol 20 No 2 (2019)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (550.32 KB) | DOI: 10.19184/jid.v20i2.8174


Aedes aegypti is the vector of various arthropod-borne diseases such as dengue fever, chikungunya and currently, zika. This study aimed to evaluate Artemisia vulgarisas other adulticides for controlling Ae. aegypti. Indonesian ministry of health hasbeen reported that more than 70.000 dengue fever human cases have occurred in 2015 covering 34 provinces which mean that dengue fever has spread now into all national territories. Eradication of Aedes still largely depends on insecticides, which is the most cost-effective strategy, and often inefficient due to resistance development in exposed Aedes population This study was designed to use of Centers for Disease Control and Prevention (CDC) bioassay standard. CDC bottles were coated with the ethanolic solution of extract with a concentration of 10, 50, 100, 500, 1000, 5000, 10000, 50000, and 100000 g per bottle. F0 mosquitoes were used for all experiments. Death and surviving mosquitoes were evaluated based on CDC standard assay. The test was performed with 10-25 adult mosquitoes every bottle and each concentration was repeated in triplicates. The results showed that LC50 and LC90 were 5790 g and 52110 g respectively after 120 minutes exposure to the extract. There was no mortality in ethanol control group. A. vulgaris significantly (P < 0.05) have adulticidal activity against Ae. aegypti. However, A. vulgaris have been reported to have larvacidal activity against Ae. Aegypti. These results indicated clearly that A. Vulgaris might act as the candidate of bioinsecticides for controlling Ae. aegypti. Keywords: Aedes aegypti, Artemisia vulgaris, adulticide,bio insecticide.
Characterization of envelope-transmembrane Gene of Jembrana Disease Virus Tabanan 1995 Isolate Kusumawati, Asmarani; Pratiwi, Rarastoeti; Astuti, Pudji; Hamid, Penny Humaidah
Indonesian Journal of Biotechnology Vol 15, No 1 (2010)
Publisher : Universitas Gadjah Mada

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The availability of specific and rapid detection methods is essential for monitoring the health status of farmed species, particularly in viral disease as in this case early diagnosis is a critical factor in containing disease outbreaks. Jembrana Disease Virus (JDV) is a lentivirus that causes an acute, severe disease syndrome in infected Bali cattle in Indonesia, resulting in heavy economic losses because of the high mortalities. The virus-host interaction and the modes of transmission are still unknown. The goal of the research was to designa probe candidate of Jembrana Disease Virus based on envelope-transmembrane (env-tm) gene to optimize Jembrana disease detection method. The DNA fragment derived from env-tm of JDV was used, cloned in pGEX-TM and expressed in E.coli DH 5&alpha;. Sequence analysis was conducted with BLAST programs from NCBI. Sequence analyses of the fragments of env-tm clone, indicated that it has a very closed genetic relation with 97,68% homology identity. Probe was designed based on the conserved region of env-tm using Geneious resulted in JT2 252 bp long. BLAST analyses showed that probes had high specifity to other strains of JDV in Indonesia.Key words : probe, env-tm, JDV, specifity, sensitivity.