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Combination Methods for Screening Marine Actinomycetes Producing Potential Compounds as Anticancer Farida, Yuyun; Widada, Jaka; Meiyanto, Edy
Indonesian Journal of Biotechnology Vol 12, No 2 (2007)
Publisher : Universitas Gadjah Mada

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Marine actinomycetes is a robust source of secondary metabolites including anticancer compounds . The objective of this research was to select marine actinomycetes producing potential compounds as anticancer used combination methods that consist of amplification PKS I (polyketide synthases type I) and NRPS (non ribosomal peptide synthetases) genes, analysis the diversity of secondary metabolites and genetic. Selected isolates were used for cytotoxicity assay. PKS I and NRPS genes were amplified using sets of degenerate primers. K1F and M6R were used for amplify ketosynthase and methyl-malonyl-CoA transferase modules of PKS I gene which targeted sequences 1200-1400 bp. A3F and A7R were used for amplify adenilation domains of NRPS gene which targeted sequences 700-800 bp. The diversity of secondary metabolites was analized by TLC and densitometry of ethyl acetate extracts. Genetic diversity was analized by repetitive DNA fingerprinting using BOXA1R primers. The cytotoxicity of secondary metabolites on T47D and MCF7 breast cell lines cancer was measured by MTT assay method. Fifty two marine actinomycetes isolates were screened using combination methods. Ten isolates were detected encoding both PKS I and NRPS genes, whereas 11 isolates were detected encoding the NRPS gene. The screening by analysis of secondary metabolites and genetic diversity methods were obtained 6 selected isolates for cytotoxicity assay, which consist of 3 isolates encoding both PKS I and NRPS genes and 3 isolates encoding NRPS gene.Isolate 1 had high cytotoxicity with the IC50 on T47D cell was 19 μg/ml and the IC50 on MCF7 cell was 7 g/ml. This findings suggests that combination methods were effective and efficient way to select marine actinomycetes producing potential compounds as anticancer.
Kandungan Vitamin A dan Protein Bakso Belut di Daerah Istimewa Yogyakarta Suryani, Yoni; Yuliati, Yuliati; Farida, Yuyun
Jurnal Kedokteran YARSI Vol 16, No 3 (2008): SEPTEMBER - DESEMBER 2008
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/jky.v16i3.248


The objectives of this study were to investigate vitamin A and protein contentsin eel (Monopterus albus) meat balls. Theeel meat balls were made byaspecial addition of tapioca30%, 40%, 50% and 60% of thetotal weight of eel meat. Hedonic test was conducted in order to find the levels of panellist preferences. Friedman test wasemployedto find the effect of tapioca addition on colour, flavour, texture and taste. The content of vitamin A was analysedusing HPLCmethodand proteincontentusing Micro Kjeldahl method. The result revealed that the variation of tapioca addition gavenosignificanteffect on colour performance with the significance level of 1.0. However, it gave significant effect on flavour, texture and taste preferences with the significance level of <0.05. It wasfound that the most favourite eel meat balls was the one with tapioca addition of 50% of the eel meat weight. The average of vitamin A contentwas 2,215.95mg/100 gand protein is 7.2896 mg/100 g on the most favourite eel meat balls.