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All Journal Majalah Kedokteran Bandung Acta Biochimica Indonesiana Jurnal Pengabdian Papua JURNAL BIOLOGI PAPUA
Euniche R.P.F. Ramandey
Universitas Cenderawasih
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Environmental Science Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Other

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Ligasi dan Transformasi Gen MSP1 Plasmodium falciparum Penyebab Malaria di Kota Jayapura Mawardi, Arsyam; Ramandey, Euniche R.P. F.
Majalah Kedokteran Bandung Vol 49, No 4 (2017)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (484.5 KB) | DOI: 10.15395/mkb.v49n4.1138

Abstract

MSP1 merupakan protein yang antigenik dan paling banyak diekpresikan pada permukaan merozoit ketika menginfeksi eritrosit pasien malaria sehingga banyak dikembangkan untuk desain terapi vaksin. Proses ligasi dan transformasi gen MSP1 merupakan upaya penggandaan gen untuk menghasilkan produk yang sama ketika diekspresikan. Penelitian ini bertujuan mengkloning gen MSP1 P. falciparum  dari pasien malaria tropika di Jayapura menggunakan vektor pJET1.2/blunt dan sel kompeten E. coli DH5 sehingga didapatkan perbanyakan plasmid rekombinan yang mengandung gen MSP1. Darah yang positif mengandung P. falciparum diproses secara molekuler, diawali tahapan isolasi DNA genom, amplifikasi dengan teknik PCR, ligasi ke dalam vektor pJET1.2/blunt dan ditransformasi pada E. coli DH5α dengan metode Heat Shock Transformation, diakhiri dengan konfirmasi PCR untuk memastikan tersisipkannya gen blok 2 MSP1. Hasil penelitian menunjukkan bahwa konfirmasi keberadaan gen MSP1 dalam pJET1.2/blunt dengan PCR berhasil dilakukan. Dari total 10 koloni positif  yang ditumbuhkan dalam kultur cair, kemudian diiisolasi plasmid dan dikonfirmasi dengan PCR diperoleh pita hasil elektroferogram dengan ukuran sekitar 1049 bp yang menunjukan gen MSP1 dalam plasmid. Berdasar atas hasil tersebut, kloning gen MSP1 menggunakan vektor kloning pJET1.2/blunt dan sel kompeten E. coli DH5a telah berhasil dilakukan. Kata kunci: Heat Shock, ligasi, MSP1, P. falciparum, transformasi Malaria-causing MSP-1 Plasmodium falciparum Ligation and Transformation in Jayapura CityMSP1 is the most antigenic and expressed protein on merozoite surface when it infects the erythrocytes of malaria patients which leads to its use for vaccine therapy design development. The ligation and transformation process of the MSP1 gene is a gene duplication attempt for producing  the same product during expression. This study aimed to clone P. falciparum MSP-1 gene from tropical malaria patients in Jayapura using pJET1.2/blunt vectors and E. coli DH5a competent cells, to get the recombinant plasmid propagation of MSP1 gene. Blood that was positive for P. falciparum was molecularly processed, starting with genomic DNA isolation and then followed by PCR amplification, ligation into pJET1.2/blunt vector, and transformation into E. coli DH5α using the heat shock transformation method. The process was ended with PCR confirmation to confirm MSP1 gene insertion. The results showed that the presence of the  MSP1 gene in pJET1.2/blunt was successfully confirmed through PCR. From a total of 10 positive colonies grown in liquid culture,  plasmid was isolated. Electropherogram result presented bands  of about 1049bp, indicating the presence of the MSP1 gene in plasmid. Hence, MSP1 gene cloning using pJET1.2/blunt cloning vector and competent cell E. coli DH5α has been successfully performed. Key words: Heat shock, ligation, MSP-1, P. falciparum, transformation  
Cloning and expression of Plasmodium falciparum lactate dehydrogenase (PfLDH) in Escherichia coli BL21(DE3) Fifi Fitriyah Masduki; Yeni Hotimah; Rosalia Rani; Arsyam Mawardi; Euniche R.P.F. Ramandey; Azzania Fibriani; Sony Suhandono
Acta Biochimica Indonesiana Vol. 2 No. 2 (2019): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.v2i2.39

Abstract

Background: Immediate and accurate diagnosis of malaria is essential for effective control of this disease. Immunochromatographic based rapid diagnostic tests (RDTs) are economical, simple to perform, and provide results in a relative short time, can be useful to assist effective management of malaria. The commercially available malaria RDT in Indonesia is still imported. Therefore, an effort to produce malaria RDT independently is necessary. One of the biomarkers used in RDTs is Plasmodium lactate dehydrogenase pLDH. The production and accumulation of pLDH during asexual stage or blood-stage in all human infected malaria parasites can be used to indicate parasites viability, which is correlated with the number of parasites present in the plasma of infected patients. Objective: The aim of this research is to produce recombinant PfLDH in Escherichia coli BL21(DE3). Methods: PfLDH gene was cloned into pET30a expression vector to obtain a 6.2 kbp recombinant plasmid pET30a-PfLDH. E. coli BL21(DE3) was transformed with pET30a-PfLDH using the heat shock method. Then, E. coli BL21(DE3)- pET30a-PfLDH was cultured in LB broth containing 50 mg/mL kanamycin and was induced by 1mM IPTG at 37oC. Results: SDS-PAGE and Western Blot analysis showed that recombinant PfLDH was expressed with molecular mass ~30 kDa. Conclusion: Recombinant PfLDH is expressed in E. coli BL21(DE3) and can be used in further research for producing rPfLDH as a biomarker for malaria RDT development.
PEMBERDAYAAAN MASYARAKAT KAMPUNG ARSOPURA, DISTRIK SKANTO, KABUPATEN KEEROM, PAPUA MELALUI PEMBUATAN SAOS TOMAT SAGU Tri Gunaedi; Euniche R.P.F. Ramandey
JURNAL PENGABDIAN PAPUA Vol 5, No 1 (2021)
Publisher : LPPM Uncen

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1165.478 KB) | DOI: 10.31957/.v5i1.1306

Abstract

The implementation of science and technology activities on the application of making sago tomato sauce aims to provide skill to the villagers Arsopura on how to make a good sago tomato sauce healthy. The skill of production tomato sago sauce was given by the demonstration of resource persons and repetition by the participants with five variations of sago tomato sauce composition. Assesment relating to sago tomato sauce products that have been produced are done by organoleptic testing. In other hand was determined pH, and viscosity of sago tomato sauce processing. Organoleptic test deals with the fondness of color, odor, aroma and taste with very very like, very likes, likes,rather likes, dislikes and very dislike criteria of the five variations of tomato sago sauce produced. Test results obtained sago tomato sago with a mixture of 100 and 150 grams of curly chili is very favorable to the respondents. It is recommended to produce sago tomato sauce should there be a mixture of enough curly chili to have the taste in accordance with the consumer like.Key words: sago, tomato and sauce
PEMBUATAN EMBEDING/BIOPLASTIK HEWAN INVERTEBRATA DI BAKAL SEKOLAH ALAM “ISYO HILLS” KAMPUNG WISATA RHEPANG MUAIF NIMBOKRANG, KABUPATEN JAYAPURA Evie Lilly Warikar; Euniche R.P.F. Ramandey
JURNAL PENGABDIAN PAPUA Vol 6, No 1 (2022)
Publisher : LPPM Uncen

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (976.544 KB) | DOI: 10.31957/jpp.v6i1.1593

Abstract

The high biodiversity in Papua can be used as biology learning material and an educational laboratory model for students in Repang Muaif Village. The diversity of animals, plants and microorganisms can be introduced or shown easily in the classroom to students with a learning medium. Facts in the field show that most teachers still provide biology material verbally without adequate media or teaching aids. This kind of learning model will inhibit the development of reasoning, interest and motivation of children to learn biology. Most biology teachers carry out learning without props because they do not have much time to provide it or do not have adequate tools / materials in school. Teachers at the nature school in the Rhepang Muaif should be able to take advantage of the existence of invertebrates in the forest around the school as learning media or teaching aids for their students. One effort that can be made to develop instructional media in schools is to introduced a technique for making invertebrate bioplastics/embedding. The purpose of this training was to introduce and practice directly the collection techniques and to introduced a technique for making invertebrate bioplastics/embedding to participants in Rhepang Muaif. This training was held on 7-9 September 2020 in Rephang Muaif Village, Nimbokrang, Jayapura-Papua. The target audience involved in this activity are young people who will become prospective teaching staff at this natural school. They come from the Rhepang Muaif and from several communities in Jayapura. The training was held for 3 days, involving 53 participants. The material provided was a technique of collecting invertebrate animals in the environment around the house yards, forests and gardens in Rhepang Muaif Village. Furthermore, making invertebrates bioplastic/embedding media obtained from the field by following the existing procedures to produce bioplastic/embedding media. The training which was conducted for the teaching staff and management of the Nature School in Rephang Muaif Village has been going well. The enthusiasm of the participants in participating in this training was very high. This indicates that the training participants have responded positively to the activities that have been carried out. Keywords: :  Training, Embedding/Bioplastics, Invertebrate Animals, Rephang Muaif 
PENGELOLAAN SAMPAH ANORGANIK MENJADI ANEKA KREASI DAUR ULANG BAGI ANAK REMAJA (PAR) DAN PEMUDA (PAM) JEMAAT GKI SILOAM WAENA, KOTA JAYAPURA Euniche R.P.F. Ramandey; Evie L. Warikar
JURNAL PENGABDIAN PAPUA Vol 5, No 2 (2021)
Publisher : LPPM Uncen

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (986.31 KB) | DOI: 10.31957/.v5i2.1585

Abstract

Garbage is one of the serious environmental problems faced by developed and developing countries. The objectives of implementing community service activities are: (1) increase the knowledge of teenagers and the youths members of GKI Siloam Waena church, about waste management through the introduction and practice of inorganic waste management directly into various recycled creations, and (2) increase the awareness of teenagers and the youths members of GKI Siloam church, Waena about waste management by empowering them in managing inorganic waste into various recycled creations. Lecture and demonstration methods used in this activity. The steps are as follows: (1) lectures to deliver material about waste, types of waste, sources of waste, waste management and 3R (reduce, reuse, recycle), and inorganic waste management into various recycled creations, (2) followed by questions and answers, (3) a demonstration of the procedures or techniques for making preparations from inorganic waste by showing and using supporting tools to facilitate training participants in working, and (4) the practice of making various recycled creations. The overall activity has been successful and has been going well.Keywords: :  environmental, management, inorganic waste, jayapura, 3R 
Keanekaragaman Ngengat Superfamily Bombicoidea: Eksplorasi Potensi Ulat Sutera di Dataran Rendah Kabupaten Jayapura Hendra K. Maury; Marsi A. Purwadi; Euniche R.P.F. Ramandey; John R. Bisai; Bruce H.R. Mauri
Jurnal Biologi Papua Vol 14, No 2 (2022)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (367.747 KB) | DOI: 10.31957/jbp.2352

Abstract

Jayapura Regency is one of the regions that considerable potential for economic growth from non-timber forest products. In the development of non-timber forest products in the form of products produced by insects, silk yarn is one of the commodity that has a high demand. The domestic demand for silk yarn is 900 tons/year, while only 19.05 tons/year were produced in 2012. Jayapura Regency has a great potential to supply the shortage of cocoon for the domestic market and the wide open international market. Related to this challenge, this study conducted basic research on the diversity of moths from the Bombicoidea superfamily in the natural forest of Kampung Soaib, Jayapura Regency. Sampling using light traps, four species of moths from the Saturnidae family were obtained, Syntherata apicalis, Opodiphtera intermedia, Opodiphtera papuana, and Coscinocera hercules. The cocoon of Coscinocera hercules is very potential as a silk thread producer because it is large and consists of two layers with brown color and has fiber characteristics similar to the cocoon of Attacus atlas which has been developed as a wild silk producer. These results indicate that the lowland forests of Jayapura Regency as a habitat for wild silkworm species has potential to be developed as non-timber forest products.Key words: Superfamily Bombicoidea; silkworm; cocoon; Jayapura regency
Analisis Dimorfisme Kupu-Kupu Sayap Burung (Ornithoptera sp.) Endemik Papua Evie L. Warikar; Euniche R.P.F. Ramandey; Hendra K. Maury
Jurnal Biologi Papua Vol 11, No 1 (2019)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2001.735 KB) | DOI: 10.31957/jbp.634

Abstract

Ornitophera sp. is one of the groups of butterflies endemic in Papua and West Papua provinces. The significant morphological differences between male and female butterflies (dimorphism) are in the shape, size and color of the wings. Morphological characteristics are important sources of information. Based on the results of specimen collections at the Papua Insect Collection Laboratory (KSP) Jayapura, there are several species from various locations in Papua. The variation in dimorphism is likely to occur in this species which gives rise to new sub-species. The purpose of this study was to record the dimorphism of the endemic Bird Wing of Papua which had been stored in the Papua Insect Collection Laboratory (KSP) Jayapura. The method used is a direct measurement of the morphology of bird wing butterfly specimens stored in the KSP Laboratory, Cenderawasih University. Morphometric data were analyzed using the SPSS 20 and Multi Variate Statistical Package (MVSP 3.1) programs. The results obtained were 7 Ornithoptera species stored in the KSP Jayapura Laboratory, namely O. chiamera, O. goliath, O. meridionalis, O. paradisea, O. priamus, O. thitonus and O. rothschildi. Based on observations there are striking differences in color, shape and size between male and female in the same species.   Key words: birdwing butterfly, Ornithoptera sp., KSP Jayapura, dimorphisme.  
Co-Authors Arsyam Mawardi Arsyam Mawardi, Arsyam Azzania Fibriani Bruce H.R. Mauri Evie L. Warikar Evie L. Warikar Evie Lilly Warikar Fifi Fitriyah Masduki Hendra Kurniawan Maury John R. Bisai Marsi A. Purwadi Rosalia Rani Sony Suhandono Tri Gunaedi Yeni Hotimah