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Evaluation of Adaptation and Production of Three Selected Cassava (Manihot esculenta Crantz) in Peat Land Area of Central Kalimantan Fitriani, Hani; Hartati, N. Sri; Sudarmonowati, Enny
Jurnal ILMU DASAR Vol 20 No 2 (2019)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (178.553 KB) | DOI: 10.19184/jid.v20i2.7449


Along with the increasing the of the land-use change of the fertile agricultural land in Java, the better awareness has been subjected to the importance of function peat land for agricultural development. Cassava (Manihot esculenta Crantz) is a tuber plant that potentially developed as important carbohydrate source. The objective of this research was to identify superior cassava variety candidate of selected cassava developed by of Research Centre Biotechnology, LIPI which has high adaptation capability and yield in Central Kalimantan peat land area. This research was conducted from July 2010 through May 2011 in Central Kalimantan Province (Kalampangan dan Pulang Pisau in total of area 1.5 and 2.25 ha respectively). There were three genotypes of cassava tested, i.e. Iding (high amylose), Gebang (low amylose), and Menti (high starch) compared to Adira 4 (35 ton/ha) and Darul Hidayah (102.1 ton/ha). The variables observed were plant height (cm) and tuber weight (grams). Observations were conducted at the age of 10 months. Data was analyzed using SPSS version 16.0. The results showed that the plant height and yield were higher in Pulang Pisau than Kalampangan even though the difference is not significant (P ≤ 0,05). Darul Hidayah has highest plant height and yield, 229.8 cm and 2271 g respectively among other genotypes/varieties grown at Pulang Pisau even though the value was not significant, whereas in Kalampangan, Darul Hidayah showed the lowest yield (670 g). Based on the data obtained, the Darul Hidayah variety can be cultivated by farmers in Pulang Pisau peat land to fulfill the availability of superior cassava seedlings for food, feed and industry. Keywords: Peat, tuber yield, plant height, liquid organic fertilizer, yam wood.
Morphological Variation and Beta Carotene Contents of Several Clones of Ubi Kuning Cassava Genotype Derived from Irradiated Shoot in vitro Rahman, Nurhamidar; Supatmi, Supatmi; Fitriani, Hani; Hartati, N Sri
Jurnal ILMU DASAR Vol 21 No 2 (2020)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v21i2.9307


In line with the increase in cassava production and the development of nutrient-rich cassava in order to support national food diversification and biofortification programs, the selection of selected varieties of cassava varieties, which are superior in nutrients especially rich in beta carotene is very necessary. Beta carotene is an important source of antioxidants to scavange free radicals and is a provitamin A precusor to form vitamin A. The development of superior cassava riching in beta carotene can be done through the mutation approach with gamma irradiation. The observation of changes in morphological characters and levels of beta carotene from irradiated cassava need to be done to get the superior beta carotene cassava clone candidates, which could be developed in the future. This experiment was conducted at the Biotechnology Research Center, LIPI. The sample used in this study originated from in vitro shoots from several Ubi Kuning clones resulting from 10 Gy radiation, which were then transferred to the field. Observations of morphological characters and levels of beta carotene of Ubi Kuning were carried out in the third generation, which was harvested at the age of 10 months. The morphological analysis of irradiated Ubi Kuning showed that there was a difference in the intensity of tuber color between some of irradiated Ubi Kuning clones compared to the control. The determination of beta carotene levels based on the standard beta carotene curve found that the highest content of beta carotene was found in the UK Rad 3.4 clone with beta carotene content of 0.252 μg / mL compared to the control (0.219 μg / mL). The lowest beta carotene content was obtained in UK Rad 3.3 (0.048 μg / mL), followed by UK Rad 3.2 (0.221 μg / mL) and UK Rad 4.1 (0.120 μg / mL). This shows that the irradiated Ubi Kuning at dosage of 10 Gy caused variations in the intensity of tuber colors and the content of beta carotene from the cassava.Keywords: Ubi Kuning, beta carotene, Gamma light irradiation
Growth and yield performance of four generations of high amylose transgenic Adira 4 cassava Sudarmonowati, Enny; Fitriani, Hani
Jurnal Ilmu Pertanian Vol 6, No 1 (2021): April (On Progress)
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.59418


Attempt to improve cassava starch quality have been conducted by producing the first high amylose transgenic Adira 4 Indonesian cassava. To evaluate agronomic performances of those transgenic plants, assessment of four generations in specific containment screenhouse and limited field trial were conducted with improved method. The survival and growth rate of cuttings was enhanced by applying growth regulator such as Atonik and improving planting procedures. The growth and yield performances were measured by comparing related variables across four generations of selected lines. Dipping in 1 mL.L-1 Atonik solution with  shading in the early planting were only required when the plant growth was poor and not favourable soil and weather. In this study, the result showed that survival of third and fourth generations of Lines A55-5 and A55-15 could reach 100 % when it was treated with Atonik. According to 14 lines assessed, Line A55-5 was one of the promising one due to its growth, multiplication performance and almost double amylose content compared to wild type, while Line A55-12 has the highest yield. The finding will benefit functional food industries when appropriate gene construct and approaches are applied for better growth and yield results. 
The Survival Rate and Starch Histochemical Assay of Various Stem Cutting Conditions of Mentega 2 Cassava Genotype at Initial Growth Stage Hartati, Sri; Fitriani, Hani; Taryana, Nanang; Nawawi, Nawawi; Cahyani, Yani; Kurniawati, Siti; Sudarmonowati, Enny
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/j.bes/515612


Background: This study aimed to evaluate the growth ability of cassava mini stem cuttings with different node number and a variety of stem cutting shapes and their correlation with starch content in the stems at initial growth stages. Methods. In this study, the viability of cassava stem cuttings was identified in two type experiments i.e. mini-stem cuttings consisting 1 and 2 nodes and shape variation of single node mini-stem cutting. Parameters observed were shoots emergence period, number of sprouting cuttings, shoots number of individual stem cuttings, shoots height and number of leaves. In addition, starch histochemical test was also carried out on stems of young shoots and initial stem cuttings using Lugol’s solution. Results. Both cassava stem cuttings consisting of 1 and 2 buds indicated the same survival rate of 100%. 1 bud stem cuttings with different shapes showed different survival rate, i.e. 60-80% for semicircular and fully circular cuttings and 30-40% for box shape cuttings. The difference in survival rate with different stem size is probably related to the availability of the amount of starch to support shoots growth. Observations at week 3 after planting generally showed that the stem cuttings with 2 buds were higher than those of stem with 1 bud. Conclusion: There were differences in the scores on the starch content test qualitatively with Lugol staining, in various parts of the plant originating from 1 bud and 2 bud cuttings which may indicate a breakdown of starch during shoot development.
The Influence of Various Growth Regulators on Induction Organogenic Callus from Gajah and Kuning Cassava Genotype (Manihot esculenta Crantz) Rahman, Nurhamidar; Fitriani, Hani; Rahman, Nurhaidar; Hartati, N. Sri
Jurnal ILMU DASAR Vol 22 No 2 (2021)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v22i2.9305


Kuning and Gajah genotypes are two collections of cassava in the Biotechnology Research Center for Germplasm, LIPI with the advantages of each genotype are high beta carotene and high production. The multiplication in in vitro culture can be done one of them through organogenesis. The aim of this study was to evaluate the effect of using 2,4-D; NAA and Kinetin are used singly for the formation of organogenesis of cassava in the Kuning Cassava and Gajah genotypes. This research was conducted at the Laboratory of Molecular Genetics and Modification of Plant Biosynthetic Pathways, Biteknologi Research Center, LIPI, Bogor since January - February 2018. The source of explants were young leaves and petiols from cassava plant culture in vitro genotypes of Gajah and Kuning yam which were three months old. in culture. The basic media used as a planting medium were Murashige and Skoog (MS) media with the addition of growth regulators (ZPT) singly, 2,4-D, NAA and Kinetin with two concentrations of ZPT each, 8 and 10 mg L- 1 This research was arranged based on a completely randomized design factorial pattern consisting of 2 factors. All data obtained were analyzed using ANOVA and if there is an influence then proceed with the DMRT test with an error rate of 5% using the SPSS program. The highest number of Kuning genotype cassava organogenic callus that developed into shoots on the medium added by ZPT was 2.4 D and kinetin with the same concentration of 8 mg L-1. Formation of the best organogenic callus in petiol explants in the media with the addition of a single 2,4-D and Kinetin with the same concentration of 8 mg L-1. Keywords: Cassava, growth regulators, organogenic.
Indonesian Collaboration Journal of Community Services Vol. 2 No. 1 (2022): Indonesian Collaboration Journal of Community Services
Publisher : Yayasan Education and Social Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53067/icjcs.v2i1.55


Abstract Home industry is a business unit or company on a small scale that is engaged in certain fields. Glass Chips Business (KIRCA) is one of the businesses that is well known in the Subang City area. Especially Pasanggrahan Village and its surroundings.  The Home Industry's role is very meaningful in empowering the community to have independence in the economic field.  18/06 Pasanggrahan Village Kasomalang Subang District, which was the first originator of the famous glass chip business. A good business strategy is needed to deal with increasingly complex and competitive business competitiontacle in facing development and marketing.  Until the time of the covid 19 pandemic arrived, it became an obstacle and obs The objectives of this research are (1) To determine the effect of internal environmental conditions and external environmental conditions on the development of businesses made from cassava.  (2) To find out the improvement of the community's economy, (3) To find out what needs to be considered to make and process cassava cassava into glass chips during the current pandemic.  The research method used in the research is qualitative analysis. And an effort to overcome this problem is to find new innovations in its manufacture and marketing strategies through appropriate informations technology.  This research was conducted in Pasanggarahan Village, Kasomalang District, Subang Regency.  The data collection technique in this study was conducted by interview and then analyzed by SWOT. The sampling method was random sampling.  The type of data consists of secondary primary data. Aggressive empowerment application means that the glass chip business is in a very profitable situation or has the opportunity and strength.