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Keragaman Morfologi dan Genetik Padi Gogo Lokal Asal Banten Mulyaningsih, Enung Sri; Indrayani, Sri
JURNAL BIOLOGI INDONESIA Vol 10, No 1 (2014): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Superior upland rice cultivar can be assembled through crossbreeding using germplasm diversity which serves as a sourceof gene. Germplasm may constitute of landrace and local cultivars. Banten is one of the provinces that has various localupland rice varieties. Baduy tribe, in the province has played a major role in preserving local upland rice varieties anduphold the wisdom.Genetic diversity information and the benefits of local cultivars can be useful to maximize the selectionprocess of parental candidate for creating new cultivars. This study aims to determine the genetic diversity of local uplandrice from Banten. Methods of analysis diversity performed by phenotypic and genotypic. Observations phenotypic throughobservation of agronomic characters and yield of each cultivar. Whereas genotypic observations conducted with RAPD(random amplified polymorphism DNA). In this study we used 17 local upland rice cultivars from Banten with 14 randomprimers for RAPD. The result showed a mean plant height local upland rice cultivar of Banten was higher than nationalupland rice varieties however productive tillers and rice productivity were lower than national varieties. Nonetheless, localcultivars persisted in this environment because it has the advantage that the community needed. Based on the time ofharvest, local cultivar belonged to early and medium maturing group. Qualitative trait observation showed that there werediversity of stem, leaves, grain and rice among the cultivars. Genetic analysis with RAPD showed nine primers out of 14were successful in amplifying loci from DNA template. The primers having high amplification product were OPJ 05, OPJ07 and OPD07 but the highest polymorphism presentation was OPJ 01. Based on phylogenetic tree, there were two mainclusters. Padi Koneng and Carogol were similar. Three glutinous rice cultivars (Mayang, Jalupang, and Hideung) were inone cluster except Kentan Simpai.Keywords: upland rice, Banten, RAPD, Phenotipe, genotype

Phenotypic Screening of Ds transposon and Activation-tag Insertional Mutant Rice Population for Drought and Salinity Tolerant Related Traits Nugroho, Satya; Zannati, Anky; Situmorang, Apriadi; Windiastri, Vincentia Esti; Widyajayantie, Dwi; Pantouw, Carla F.; Astuti, Dwi; Indrayani, Sri; Rahmawati, Syamsidah; Mulyaningsih, Enung Sri; Santoso, Tri Joko; Trijatmiko, Kurniawan Rudi
ANNALES BOGORIENSES Vol 15, No 1 (2011): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/56

The studies of rice genes through functional genomics are greatly facilitated by the availability of the complete genome sequences, including the complete physical map of the japonica rice cv. nipponbare. Using the Ac and Ds transposon, that are capable of transposition in various heterologous plants including the monocotyledon rice, Â combined with the enhancer element for generating activation taged lines, it is possible to discover and isolate functional genes involved in various important agronomical traits; such as those involved in abiotic stress tolerance (drought, high salt) and biotic stresses diseases and pests. We have developed 1,785 first (T0) generation of mutant nipponbare rice by transposons Ac/Ds insertions containing activation-tag, generated by transformation using Agrobacterium-mediated method. Currently, we have generated approximately 1,000 stable lines with transposon Ds and activation-tag insertion ready for screening. Efficient screening methods for mutant Nipponbare rice lines have been established for agronomically important mutant traits. Among the new phenotypes related to important agronomical traits observed were drought and salt tolerant or sensitive, stunted and robust growth, variable root penetration and other interesting traits such as reduced tillering, rolled leaf and thin tiller. Â Â Keywords: rice, insertion mutant, gene discovery, drought, salinity

KEGIATAN MEMBUAT MOZAIK DENGAN MEDIA KAPAS UNTUK MENGEMBANGKAN KETRAMPILAN MOTORIK HALUS ANAK Indrayani, Sri; Hermahayu, Hermahayu
Edukasi: Jurnal Penelitian dan Artikel Pendidikan Vol 5 No 11 (2013): Vol. 5 No. 11, Desember 2013
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (240.644 KB)

This research aims to know the influence of the activities of Making Mosaics fine motor Skills Against Cotton in children. This research is a research experiment with the model One Group Pre Posttest Design by performing the measurement of initialand final measurement. the subject of this research is the son of a kindergarten Chrysanthemum IX Nampurejo Purwodadi  Purworejo as much as 3 students. Sampling techniques using Total Sampling. This research using the method of data collection in the form of sheets of Observation. Data analysis techniques used are analysis techniques Test rank signed Wilcoxon with the help of the program SPSS For Windows Version 17.00. The results showed that the activity of making mosaics cotton effect on fine motor skills in children. The initial value of the fine motor skills of at least 26 children and up to 31. Then after being given the treatment makes the final measurement value cotton mosaic of at least 57, 62 maximum value. Tested with the help of SPSS Test Rank Signed Wilcoxon. Statistical tests showed Asymp. SIG (2-Tailed) = 0.00102 ? = 0.005 < then formulated a hypothesis, that the activity of making mosaics cotton was able to develop fine motor skills, accepted and proven true.

HUBUNGAN ANTARA INDEKS MASSA TUBUH TERHADAP KADAR HEMOGLOBIN DAN ERITROSIT Ramadhana, Aslamnur Fikri; Indrayani, Sri; Irawan, Aurizal Risandy; Kamila, Rezkia Azka; Apriyandi, Restu Amelia; Bashirah, Danaparamita; Sarah, Dewi; Hilmi, Hanny Latifa; Rachmawati, Afina Dwi; Sinuraya, Rano Kurnia; Destiana, Dika Pramita; Wicaksono, Imam Adi
Farmaka Vol 17, No 3 (2019): Farmaka (Desember)
Publisher : Fakultas Farmasi, Universitas Padjadjaran

ABSTRAKMasalah gizi yang utama yang dialami oleh para remaja diantaranya yaitu anemia yang dapat disebabkan karena kurangnya kadar hemoglobin. Kadar hemoglobin seseorang bisa dilihat dari pola makan yang tidak teratur yang akan mempengaruhi asupan makanan yang bisa dilihat dari nilai status gizi. Status gizi seseorang bisa dipantau dengan melihat nilai Indeks Massa Tubuh (IMT). Penelitian ini dilakukan untuk mengetahui hubungan antara IMT dengan kadar hemoglobin dan eritrosit dalam tubuh pada mahasiswa Fakultas Farmasi Universitas Padjadjaran. Penilitian ini dilakukan dengan mengambil data meliputi jenis kelamin, usia, indeks massa tubuh dan pemeriksaan laboratorium kadar haemoglobin beserta eritrosit. Data yang diperoleh didistribusikan dalam bentuk distribusi frekuensi kemudian ditampilkan dalam bentuk presentase dan disajikan dalam bentuk tabel dan diagram kemudian dilakukan analisis statistik menggunakan Normalitas Data Shapiro Wilk dan kemudian dilakukan Uji Korelasi Chi-Square. Didapatkan hasil Uji Pearson Chi-Square dengan nilai signifikansi antara BMI dan Hb Sig. (2-tailed) > 0.05 yaitu 0.170Â dan nilai signifikansi antara BMI dan eritrosit Sig. (2-tailed) > 0.05 yaitu 0.837, maka dapat dikatakan untuk keduanya tidak terdapat hubungan linear antara BMI dengan Hb maupun Eritrosit.Â Kata Kunci: Indeks Massa Tubuh, Hemoglobin, Eritrosit

KERAGAMAN MORFOLOGI DAN GENETIK PADI GOGO LOKAL ASAL BANTEN Mulyaningsih, Enung Sri; Indrayani, Sri
JURNAL BIOLOGI INDONESIA Vol 10, No 1 (2014): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v10i1.337

Superior upland rice cultivar can be assembled through crossbreeding using germplasm diversity which serves as a sourceof gene. Germplasm may constitute of landrace and local cultivars. Banten is one of the provinces that has various localupland rice varieties. Baduy tribe, in the province has played a major role in preserving local upland rice varieties anduphold the wisdom.Genetic diversity information and the benefits of local cultivars can be useful to maximize the selectionprocess of parental candidate for creating new cultivars. This study aims to determine the genetic diversity of local uplandrice from Banten. Methods of analysis diversity performed by phenotypic and genotypic. Observations phenotypic throughobservation of agronomic characters and yield of each cultivar. Whereas genotypic observations conducted with RAPD(random amplified polymorphism DNA). In this study we used 17 local upland rice cultivars from Banten with 14 randomprimers for RAPD. The result showed a mean plant height local upland rice cultivar of Banten was higher than nationalupland rice varieties however productive tillers and rice productivity were lower than national varieties. Nonetheless, localcultivars persisted in this environment because it has the advantage that the community needed. Based on the time ofharvest, local cultivar belonged to early and medium maturing group. Qualitative trait observation showed that there werediversity of stem, leaves, grain and rice among the cultivars. Genetic analysis with RAPD showed nine primers out of 14were successful in amplifying loci from DNA template. The primers having high amplification product were OPJ 05, OPJ07 and OPD07 but the highest polymorphism presentation was OPJ 01. Based on phylogenetic tree, there were two mainclusters. Padi Koneng and Carogol were similar. Three glutinous rice cultivars (Mayang, Jalupang, and Hideung) were inone cluster except Kentan Simpai.Keywords: upland rice, Banten, RAPD, Phenotipe, genotype

Parameter Genetik Karakter Agronomi pada Galur F1 Padi Hasil Persilangan Galur Murni dan Kultivar Lokal Indonesia Adi, Eko Binnaryo Mei; Indrayani, Sri; Burhana, Nana; Mulyaningsih, Enung Sri
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 5 No 1 (2021): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/agrosainstek.v5i1.143

Rice is one of the main sources of carbohydrates for Indonesian society. The development of new varieties depends on the availability of germplasm as the source genetic material. The development of new varieties can be done through artificial crosses (local cultivars and superior lines) for the creation of diversities. The research aimed to explore the genetics parameters from eight rice characters, for the selection program. The crossing was conducted by using four local cultivars and one pure line with diverse agronomic characters. Four hybrids (F1) were planted using completely randomized design with four replications, and five parental as the check varieties (landrace and pure line). The correlation coefficient was found that there was one character showed strong correlation almost all the character that is panicle weight. While high heritability was found in harvest age, panicle length, number of grain per panicle, 1000 grain weight, and plant high. Hybrids that showed negative heterosis for harvest age are from B14081H-296 x Salak and Dampak x Carogol, which have shorter harvest age compared to the average of the parental plant which is dominant gene action and additive.

RESPONSE OF SIX CHILI VARIETIES TO ANTHRACNOSE DISEASE CAUSED BY Colletotrichum acutatum AND C. gloeosporioides Perdani, Ambar Yuswi; Paradisa, Yashanti Berlinda; Wahyuni, Wahyuni; Indrayani, Sri; Sulistyowati, Yuli; Cahyani, Yani
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 21 No. 2 (2021): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.221144-150

Response of six chili varieties to anthracnose disease caused by Colletotrichum acutatum and C. gloeosporioides. Chili is one of the horticultural commodities with high economic value. Chili production is constrained by anthracnose diseases. Losses due to anthracnose can reduce the fruits quality and yields lose. This study aims to determine the resistance of several chili varieties to anthracnose. Genetic material was used six varieties of chili. Ripe chilies were inoculated with two types of Colletotrichum isolates, i.e. C. acutatum and C. gleosporides. The experiment was arranged in a factorial randomized block design with three replications. The first factor were chili varieties: Laris, SSP, Habanero, Cibinong, Ekasari, and Kopay. The second factor was two types of fungal isolates. Observations were made on the incidence and severity of disease due to anthracnose. The results showed that both fungal isolates were effective in causing anthracnose disease in chilies. Habanero was very susceptible to anthracnose. Laris and Ekasari were moderately resistant to anthracnose diseases. These findings are important to develop new Capsicum cultivars that are more adaptive to anthracnose disease.

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