Tutung Hadiastono
Jurusan Hama dan Penyakit Tanaman, Fakultas Pertanian, Universitas Brawijaya

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PENGARUH PENGGUNAAN PGPR (PLANT GROWTH PROMOTING RHIZOBACTERIA) TERHADAP INTENSITAS TMV (TOBACCO MOSAIC VIRUS), PERTUMBUHAN, DAN PRODUKSI PADA TANAMAN CABAI RAWIT (Capsicum frutescens L.) Kamila Qurota A’yun; Tutung Hadiastono; Mintarto Martosudiro
Jurnal HPT (Hama Penyakit Tumbuhan) Vol. 1 No. 1 (2013)
Publisher : Universitas Brawijaya

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One of the main disease of chili cultivation is mosaic disease that is caused byTMV (Tobacco Mosaic Virus). One effort to control the disease could be done byapplying Plant Growth Promoting Rhizobacteria (PGPR). PGPR is a usefulbacteria that lives and grows well in soil that contain much of organic matter. Thisresearch aimed to determine the effect of application and the role of PGPRPseudomonas fluorescens, Azotobacter sp., dan Bacillus subtilis on TMV attackintensity, the height and production of chili plant. This research was conducted inscreen house and plant disease laboratory, Brawijaya University Malang. Theexperiment started in April until August 2012. This research was using completerandomized design. The treatments used one species of PGPR bacteria and thecombination of two species of PGPR bacteria. The PGPR were PGPR P.fluorescens, PGPR Azotobacter sp., or PGPR B. subtilis. Whereas the PGPRcombination were PGPR P. fluorescens and PGPR Azotobacter sp., PGPR P.fluorescens and PGPR B. subtilis, or PGPR Azotobacter sp. and PGPR B. subtilis.Control were executed without applying PGPR. Results showed that thecombination of P. fluorescens and Azotobacter sp. PGPR affected on reducing theincubation periode, the attack intensity of TMV, and increasing the plant height.The incubation periode for chili plants with PGPR P. fluorescens and Azotobactersp. was 16,67 days. The PGPR P. fluorescens and Azotobacter sp. reduced TMVattack intensity on chili plants until 89,92%. The plant height of chili plants withPGPR P. fluorescens and Azotobacter sp. could reach 69,25 cm. PGPR P.fluorescens dan B. subtilis treatment could increase the average fruit weight ofchili until 2,17 grams per plant.Keywords: PGPR, Pseudomonas fluorescens, Azotobacter sp., Bacillus subtilis,TMV, chili
KETAHANAN LIMA VARIETAS TANAMAN CABAI RAWIT (Capsicum frutescens L.) TERHADAP INFEKSI TMV (TOBACCO MOSAIC VIRUS) PADA UMUR TANAMAN YANG BERBEDA Dian Eka Kusumawati; Tutung Hadiastono; Mintarto Martosudiro
Jurnal HPT (Hama Penyakit Tumbuhan) Vol. 1 No. 1 (2013)
Publisher : Universitas Brawijaya

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Abstract

Diseases caused by TMV (Tobacco Mosaic Virus) on chili is important to notebecause it can cause a loss. Chili varieties are currently circulating in the marketand have not been evaluated resistance to TMV (Tobacco Mosaic Virus).Therefore, research is needed on the effects of the attack TMV (Tobacco MosaicVirus) on the growth, production and resistance to chili varieties such. The initialsymptoms are the same, namely the emergence of a mosaic on the leaves of youngplants, followed by chlorosis due to reduced chlorophyll content of plants forphotosynthesis disturbed then a widespread and causes the leaves begin to showsymptoms of the malformation. Leaves of the plant having malformations (leavesshriveled). The results showed that TMV infection can suppress plant growth anddecrease crop production. Treatment plant inoculation at different ages apparentlysignificant effect on plant growth as well as production plants. Chili plantsinfected with virus at a very young age the rate of plant growth and developmentis inhibited, so the plants will grow abnormally. Based on the parameters used tocalculate the resistance category chili tested against TMV virus showed that thevarieties are very susceptible Sonar and Bara, Cakra susceptible, moderatelyresistant while TM Rawit, Samaru resistant to TMV infection.Keywords: Chili plants, TMV, inoculation, infection and resistance
KETAHANAN LIMA VARIETAS TANAMAN SAWI HIJAU (Brassica juncea L.) TERHADAP INFEKSI Turnip Mosaic Virus (TuMV) Esti Yuliastri Sa’idah; Mintarto Martosudiro; Tutung Hadiastono
Jurnal HPT (Hama Penyakit Tumbuhan) Vol. 1 No. 4 (2013)
Publisher : Universitas Brawijaya

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Turnip Mosaic Virus (TuMV) adalah virus yang sering menginfeksi tanaman sayuran Brassicacea. TuMV merupakan virus baru pada tanaman Brassicaceae di Indonesia, dan berpotensi menjadi penyakit penting. Penularannya terjadi secara mekanis dan melalui serangga vektor. Varietas sawi komersial di Indonesia belum diuji ketahanannya terhadap infeksi TuMV. Penelitian ini bertujuan untuk mengetahui ketahanan lima varietas tanaman sawi terhadap infeksi TuMV, dan mengetahui pengaruh infeksi TuMV terhadap pertumbuhan dan produksi tanaman sawi. Penelitian dilaksanakan di Laboratorium Penyakit Tumbuhan, Jurusan Hama Penyakit Tumbuhan, Fakultas Pertanian, Universitas Brawijaya Malang dan di screenhouse Universitas Muhammadiyah Malang. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan lima perlakuan varietas sawi dan tiga ulangan. Hasil penelitian tingkat ketahanan beberapa varietas tanaman sawi terhadap infeksi TuMV adalah varietas Lokal Malang dan Toksakan merupakan varietas rentan, sedangkan varietas Shinta, Majapahit, dan Dora merupakan varietas yang tahan. Infeksi TuMV mempengaruhi pertumbuhan dan produksi tanaman sawi, yakni mengakibatkan pengurangan luasan daun, panjang akar, dan bobot basah tanaman. Kata Kunci: ketahanan, varietas, sawi, Turnip Mosaic Virus (TuMV)
PENGARUH KONSENTRASI Spodoptera litura Nuclear Polyhedrosis Virus (SlNPV) JTM 97 C TERHADAP EFEKTIVITAS PENGENDALIAN Crocidolomia binotalis Zell PADA TANAMAN KUBIS (Brassica oleraceae Var. Botrytis L) M. Guruh Arif Zulfahmi; Tutung Hadiastono; Mintarto Martosudiro; Bedjo Bedjo
Jurnal HPT (Hama Penyakit Tumbuhan) Vol. 3 No. 2 (2015)
Publisher : Universitas Brawijaya

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Penelitian ini bertujuan untuk mengetahui pengaruh perbedaan konsentrasi (PIBs) SlNPV-JTM 97 C  terhadap waktu berhenti makan, mortalitas larva C. binotalis. Penelitian dilaksanakan di laboratorium Entomologi BALITKABI Malang, pada bulan  Februari sampai Mei 2014. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan 5 perlakuan dan 4 ulangan. Perlakuan yang diujikan yaitu SlNPV-JTM 97 C dengan konsentrasi 106, 108, 1010, 1012 (PIBs/ml) dan kontrol (tanpa SlNPV). Parameter pengamatan adalah waktu berhenti makan dan mortalitas. Data dianalisis dengan uji F, dilanjutkan uji BNT dengan taraf 5%. Waktu berhenti makan dan mortalitas  larva C. binotalis sangat dipengaruhi oleh tingkat konsentrasi virus SlNPV-JTM 97C. Konsentrasi mematikan 50% (LC50) SlNPV-JTM 97C adalah 4.9 x 104PIBs/ml pada pengamatan 168 JSI. Sedangkan waktu mematikan 50% (LT50) SlNPV-JTM 97C adalah 4,4 HSI pada konsentrasi 1012PIBs/ml. Nilai koefisien korelasinya (R) 0,997. Semakin tinggi konsentrasi PIBs yang diinokulasikan, persentase kematian larva C. binotalis semakin tinggi, dan waktu untuk mematikan juga semakin singkat. Kata kunci: C. binotalis, SlNPV, Konsentrasi, waktu berhenti makan, mortalitas.