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All Journal Buletin Peternakan Jurnal Veteriner Jurnal Ilmiah Aplikasi Isotop Radiasi Jurnal Ilmu Ternak dan Veteriner JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) JURNAL GALUNG TROPIKA Jurnal Hilirisasi IPTEKS (JHI) Jurnal Biosense

Ananda Ananda

Universitas Andalas

Author-ID : 5368032

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Dentistry Engineering Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary Other

Published : 9 Documents Claim Missing Document

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Articles

Perkembangan Sel Endometrium Domba setelah Inkubasi dalam Kolagenase dan Dikultur In Vitro dengan Estradiol dan Progesteron Ananda Ananda; Ekayanti Mulyawati Kaiin; Ni Wayan Kurniani Karja; Mohamad Agus Setiadi
Jurnal Veteriner Vol 20 No 3 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The aim of the present study was to determine the optimal incubation time of collagenase on concentration, viability, quality of endometrial cell culture, and the role of estradiol and progesterone on ovine endometrial cells proliferation. Endometrium minced was incubated into collagenase with different duration of time: 1 hour, 3 hours, and 6 hours repectively. Cell concentration and viability were calculated after incubation. The quality of cell culture was observed on 1st, 3rd, and 5th day after seeding. The best incubation result was then continued with the addition of the E2 (100 pg/ mL), P4 (100 ng/mL), E2:P4 (100 pg/mL : 10 ng/mL), and E2:P4 (10 pg/mL : 100 ng/mL) into the culture medium. After nine days, cell culture was harvested by trypsinization. Concentration and cell viability were analyzed using analysis of variance, followed by Duncan test. Quality of endometrial cell culture was analyzed descriptively. Results showed that there was a significant decreased in the concentration and cell viability obtained in each treatment of collagenase incubation time and optimum treatment of endometrial cell culture was found at 3 hours. Experiment on culture of endometrial cell revealed that proliferation rate treated by E2 and P4 was better then control (P<0.05). Futhermore, additional of E2 into the culture medium even E2 alone (100 pg/mL or higher E2 combination with P4 (100 pg/mL : 10 ng/mL) showed better proliferation rate than P4 alone (100 ng/mL) or higher P4 combination (10 pg/mL : 100 ng/mL). In conclusion, suplementation of 100 pg/mL of estradiol (E2) could support better proliferation of ovine endometrial cells in vitro.

Use of Collagenase Enzyme as a Substitute for Trypsin on In Vitro Growth of Liver, Kidney, and Fibroblasts of Chicken Embryo Cells Ananda Ananda; Ekayanti Mulyawati Kaiin
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 2 (2022): JITRO, May 2022
Publisher : Universitas Halu Oleo

This study aims to determine the effect of using the collagenase enzyme on the viability of chicken embryos' liver, kidney, and fibroblast cells in vitro. Liver and kidney organs were collected from embryonic chicken eggs at 15 and 19 days. Meanwhile, fibroblast cells were obtained using muscle tissue of 11-day-old chicken embryos. Each organ and tissue was chopped and incubated using 0.1% trypsin for 10 minutes and 0.1% collagenase for 30 minutes. Concentration, viability, and proliferation rate were calculated after incubation and analyzed using a t-test. The results showed that there was a significant increase in the tissue incubated with collagenase on the viability and proliferation rate of liver cells, kidney cells, and chicken embryo fibroblast cells (t<0.05), and not significant in the acquisition of cell concentrations (t>0, 05). Cell isolation and purification techniques need to be carried out to study the quality of somatic cell culture of chicken embryos more specifically.Keywords: collagenase, trypsin, chicken embryos, viability, cell culture.

FUNGSI REPRODUKSI DAN GAMBARAN HISTOLOGI ORGAN REPRODUKSI MENCIT (Mus musculus) BETINA PASCA PEMBERIAN KOPI Ananda; Herbert Sipahutar; Meida Nugrahalia
JURNAL BIOSENSE Vol 5 No 2 (2022): Edisi Desember 2022
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Penelitian ini bertujuan untuk mengetahui fungsi reproduksi dan gambaran histologi organ reproduksi mencit (Mus musculus) betina pasca pemberian kopi secara oral. Parameter yang diamati adalah berat badan, berat uterus, panjang siklus estrus, daya fertilisasi, jumlah folikel primer, jumlah folikel sekunder, diameter pembuluh darah ovarium, tinggi epitel endometrium, dan ketebalan endometrium dengan lama pemberian selama 21 hari dan 42 hari untuk parameter daya fertilisasi. Dosis kopi yang digunakan dalam penelitian ini adalah setara dengan tiga cangkir kopi pada manusia yang dikonversikan menjadi 0,5 ml pada mencit dan dilakukan empat kali pengulangan. Data yang diperoleh dianalisis dengan menggunakan uji t. Hasil penelitian menunjukkan pemberian kopi memberi pengaruh yang nyata terhadap jumlah folikel sekunder ovarium mencit dan tinggi epitel endometrium mencit, serta tidak berpengaruh nyata terhadap berat badan, berat uterus, panjang siklus estrus, daya fertilisasi, diameter corpus luteum, jumlah folikel primer, diameter pembuluh darah ovarium, ketebalan endometrium, dan ketebalan miometrium.

Effect of Zinc Supplementation in the Diet on Sikumbang Janti Female Duck Performance, Carcass, Digestive Organs, and Intestinal Morphology Ridho Kurniawan Rusli; Robi Amizar; Zurmiati Zurmiati; Ananda Ananda; Arif Darmawan; Kusnadidi Subekti; Khalil Khalil
Jurnal Ilmu Ternak dan Veteriner Vol 28, No 2 (2023): June 2023
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v28i2.3209

The research aimed to evaluate the effect of Zn supplementation in diet of Sikumbang Janti female duck on its performance, carcass, digestive organs, and intestinal morphology. The study used 96 female ducks aged 8 weeks. This research used a completely randomized design with four treatments (0, 30, 60, and 90 mg Zn/kg), each treatment was repeated four times. Performance, carcass, digestive organs, and intestinal morphology were observed. The results showed that Zn supplementation significantly (P0.05) affected body weight, body weight gain, feed conversion ratio, carcass weight, abdominal fat, and intestinal morphology, but it did not affect (P0.05) feed consumption, carcass percentage, and digestive organs. In conclusion, supplementation of 60 mg Zn/kg in the diet improved performance, intestinal morphology, and the health of visceral of Sikumbang Janti female ducks aged 8 to 16 weeks.

Sperm Longevity and Motility in Ringer’s Lactate Solution with Addition of Egg Yolk among Five Phenotypes of Kokok Balenggek Chicken Ananda Ananda; Jaswandi Jaswandi; Rusfidra Rusfidra; Harif Gusdinal
Buletin Peternakan Vol 47, No 3 (2023): BULETIN PETERNAKAN VOL. 47 (3) AUGUST 2023
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v47i3.83647

The application of cryopreservation to preserve germplasm in such specific breed requires preliminary studies, primarily related to the resistance of spermatozoa to low temperatures (4 – 5 °C) as measured by their motility and longevity. In this study, semen taken from five phenotypes of Kokok Balengeek Chicken (KBC) (Biriang, Jalak, Kinantan, Kuriak, and Taduang) was used to evaluate the effect of Ringer’s Lactate-egg yolk diluent on longevity and motility of spermatozoa. The treatments consisted of Ringer’s Lactate (RL) solution added with egg yolk at a concentration of 1% (RLKT1), 3% (RLKT3), and 5% (RLKT5). Evaluation of fresh semen showed that the spermatozoa of Jalak had the highest motility, namely 75.63 ± 0.5% (P<0.05). Post-dilution longevity and motility observations were carried out at 0, 24, 48, and 72 h, significantly decreasing each time (P<0.05). The lowest range of reduction was found in Jalak spermatozoa diluted with RL with longevity of 7.75 ± 0.70 days. Overall, the RL diluent showed the highest motility after 24 h, namely 41.13 ± 2.27%. Adding egg yolks to Ringer's Lactate solution could not maintain the motility of KBC spermatozoa when stored at 4-5°C for 48-72 h.

Efektifitas Herba Ciplukan (Physalis angulata L.) Sebagai Imunostimulan Kekebalan Unggas Setelah Uji Tantang Virus Newcastle Desease N Nurhasanah; Yulia Yellita; S Sabrina; A Ananda
JURNAL GALUNG TROPIKA Vol 12 No 2 (2023)
Publisher : Fapetrik-UMPAR

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31850/jgt.v12i2.1074

This research aims to determine the effectiveness of ciplukan herb (Physalis angulata L) in extract and powder preparations as an immunostimulant against the immunity of broilers who have received vaccination and tested against the Newcastle disease virus. The materials used were 96 DOC with an average weight of 35-40 g, ciplukan herb from Luhak Nan Duo, the velogenic strain of Newcastle disease virus from Bvet Lampung, and the commercial vaccine ND Lasota. The study used a completely randomized design (CRD) with 8 treatments and 3 replications. The ciplukan herb was given for 3 days with concentrations: K(-) (without vaccine without ciplukan), K+ (vaccine only), E3 (ciplukan extract 0.35mg/10ml/head + vaccine), E2 (ciplukan extract 0.25mg/head 10ml/head + vaccine), E1 (0.15mg/10ml/head ciplukan extract + vaccine), S3 (17.5mg ciplukan powder/head + vaccine), S2 (12.5mg ciplukan powder/head + vaccine) and S1 (ciplukan powder 7.5mg/head + vaccine). The parameters observed in this study were antibody titer, morbidity, and mortality. The study showed that the administration of ciplukan herb positively affected low antibody titers through the efficiency of cellular defense mechanisms. This herb can also reduce chicken mortality and morbidity after challenge tests against the Newcastle disease virus.

Exploring the Radiation Techniques in Agricultural Wastewater Management Af'ida Khofsoh; Inggit Kresna Maharsih; Ananda Ananda; Ahmad Dody Setiadi; Muhammad Hamzah Solim
Jurnal Ilmiah Aplikasi Isotop dan Radiasi Vol 19, No 1 (2023): Vol 19, No. 1 (2023): November 2023
Publisher : BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17146/jair.2023.19.1.6889

Radiation techniques have gained significant attention in the field of agricultural wastewater management due to their effectiveness in treating diverse contaminants. This review aims to explore the effects and applications of radiation techniques, including ultraviolet (UV), gamma-ray, and electron beam (EB). UV radiation utilizes ultraviolet light to break down organic pollutants, disinfect pathogens, and remove pesticides in agricultural wastewater. Besides, gamma radiation involves the use of ionizing radiation to interact with contaminants and induce degradation processes. Furthermore, EB radiation harnesses high-energy to degrade organic compounds in wastewater. The efficacy of radiation techniques in reducing pesticides, pharmaceutical residues, microorganisms or pathogens, and other organic pollutants has been widely demonstrated. These techniques offer advantages such as versatile applicability, precise targeting of contaminants, and the potential for water reuse in various agricultural sectors, such as crop irrigation, livestock farming, and food processing. However, optimizing process parameters, including radiation dose, dose rate, pH, and temperature, are crucial to maximize treatment efficiency. While radiation techniques have proven beneficial in numerous studies, potential environmental impacts must be addressed. Byproducts generated during radiation and their fate should be studied to evaluate their toxicity and persistence. Proper waste disposal, adherence to safety regulations, and monitoring programs are necessary to minimize risks and ensure the safe use of radiation techniques. In conclusion, UV-C radiation effectively use for surface disinfection, pathogen inactivation, certain pesticides and pharmaceutical residues degradation, while gamma-ray more effective than UV-C for microorganism sterilization and inactivation, pesticide and pharmaceutical residues degradation, as well as EB radiation has high dose rate and selective penetration, and the technique also has speed and precision, feasible for practical application. Thus, advancements in technology will further optimize the efficacy and sustainability of radiation-based wastewater treatment processes in agriculture.

The Quality of Frozen Buffalo Sperm Following Sexing using Bovine Serum Albumin (BSA) Column and Swim-Up (SU) Methods Masrizal Masrizal; Tinda Afriani; Dwiki Wahyudi; Savira Saharani; Ananda Ananda
Buletin Peternakan Vol 48, No 1 (2024): BULETIN PETERNAKAN VOL. 48 (1) FEBRUARY 2024
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v48i1.89394

This study assesses the impact of two sperm sexing techniques, the Bovine Serum Albumin (BSA) column method, and the swim-up (SU) method, on frozen buffalo spermatozoa quality. A total of 50 straws of frozen buffalo semen were used in this study. Spermatozoa quality was evaluated before (post thawing) and after the spermatozoa sexing process. Spermatozoa trapped in BSA upper fraction, BSA lower fraction, SU upper fraction, and SU lower fraction were separately evaluated. The parameters measured consisted of spermatozoa motility, viability, intact plasma membrane, intact acrosome cap, and spermatozoa DNA integrity. The results indicated that the quality of post-thawing buffalo spermatozoa remained relatively high, with motility at 41%, viability at 64.48%, intact plasma membrane at 55.42%, intact acrosome cap at 47.12%, and sperm DNA integrity at 74.94%. However, the use of the BSA column method significantly (p<0.05) decreased spermatozoa quality in both the upper and lower fractions, resulting in motility levels of 34% and 32%, viability rates of 49.36% and 44.71%, intact plasma membrane percentages of 44.78% and 37.13%, intact acrosome cap figures of 37.58% and 33.27%, and sperm DNA integrity levels of 74.76% and 72.45%, respectively. In contrast, the application of the SU method proved effective in preserving post-thawing spermatozoa quality, yielding motility rates of 42% and 41%, viability levels of 63.62% and 62.78%, intact plasma membrane percentages of 54.42% and 54.74%, intact acrosome cap figures of 46.94% and 45.74%, and sperm DNA integrity values of 70.57% and 70.01%, respectively. In summary, after freezing, the SU method excel the BSA column method in maintaining the quality of buffalo spermatozoa post-thawing.

PELATIHAN MANAJEMEN PAKAN DAN PEMELIHARAAN KAMBING PERANAKAN ETAWA UNTUK MENINGKATKAN KINERJA PETERNAK DIPETERNAKAN AZHAR FARM PAYAKUMBUH Rizqan Rizqan; Elly Roza; Hilda Susanty; Linda Suhartati; Ananda Ananda
Jurnal Hilirisasi IPTEKS Vol 7 No 2 (2024)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jhi.v7i2.790

Goats are one of the small ruminants as meat and milk producers (dual-purpose), but cow's milk is widely sold in the market. Goat milk is no less nutritious than cow's milk; besides having an advantage in protein content, goat milk also contains more vitamins A and B (especially riboflavin and niacin). One of the goats widely cultivated by farmers and utilized as a source of animal protein, meat, and milk is the Ettawa crossbred goat. As researchers have widely reported, the potential of Ettawa crossbred goats is that milk producers are still very diverse, ranging between 0.45 and 2.2 kg/head/day. This is generally caused by maintenance management, which still needs to be improved. The purpose of this service is to counsel Azhar Farm farms on the management of raising Ettawa crossbred goats to get maximum productivity. The methods used are counseling, training, coaching, discussion, and evaluation of service activities. The activity results include improving farmers' ability to apply the management of raising Ettawa crossbred goats, which are dairy-type goats (milk producers), and increasing their productivity. It is hoped that this activity can motivate farmers to develop their livestock business to improve livestock health, increase population, increase production, and increase income for farmers.

Co-Authors . Khalil Af'ida Khofsoh Ahmad Dody Setiadi Arif Darmawan Dwiki Wahyudi Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Elly Roza Harif Gusdinal Herbert Sipahutar Hilda Susanty Inggit Kresna Maharsih Jaswandi . Kusnadidi Subekti Linda Suhartati M Agus Setiadi Masrizal Masrizal Meida Nugrahalia Muhammad Hamzah Solim N Nurhasanah Ni Wayan Kurniani Karja Ridho Kurniawan Rusli Rizqan Rizqan Robi Amizar Rusfidra rusfidra S Sabrina Savira Saharani Tinda Afriani Yulia Yellita Zurmiati

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