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Size and Density of Artemisia annua Stomata Soaked in Water Extract of Gloriosa superba Seeds Rahmawati, Sri Indah; Yunus, Ahmad; Susilowati, Ari
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.11234

Abstract

Artemisia annua is a herbaceous plant that produces artemisinin as a malaria drug, haemorrhoids therapy, aromatherapy, antiviral, anticancer and antibacterial. Gloriosa superba is a plant that contains high colchicine compounds, especially on the seeds. Gloriosa superba extracts of tubers, stems, seeds, and leaves were used as biomutagen for many plants. Colchicine contains of these plants as antimitotic have been studied and proven by the mitotic index plants. Water extracts of Gloriosa superba seeds was used as a mutagen for Artemisia annua. The aim of this study was to determine the size and density of Artemisia annua stomata soaked in water extract of Gloriosa superba seeds as a mutagen. Extraction of Gloriosa superba seeds obtained naturally on Krakal Beach, Gunung Kidul by using a maceration method with water solvent (1:1). Artemisia annua sprouts were obtained from B2P2TOOT Tawangmangu. Variables treatment on sprouts using water extract concentration of Gloriosa superba seeds and soaking time of Artemisia annua sprouts. Measurements of stomatal length, width and density were conducted in epidermis of Artemisia annua leaf. Observation and measurements of the stomata were conducted by using a light microscope. The results showed that the length and width of stomata were 0.025 mm and 0.017 mm respectively. The stomatal density of the control leaf (174.69 amount/mm2) was lower than the other treated plants. Stomatal size and density has increased with the increasing concentration extracts on treated plants. Water extracts of Gloriosa superba seeds proved the effects on stomatal size and density of treated plants.
Diversity Induction of Dendrobium sylvanum Orchid through In Vitro Irradiation of Gamma Ray Lestari, Eka Puji; Yunus, Ahmad; Sugiyarto, Sugiyarto
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (415.351 KB) | DOI: 10.15294/biosaintifika.v10i3.16265

Abstract

Dendrobium sylvanum orchid is categorized as lowland habitus orchid and able to survive wihtout any shade. Orchid cultivation takes a very long time so it needs faster cultivation and producing large quantities. Therefore, it is necessary to do orchid culture by using tissue culture processes and then given gamma ray irradiation to bring up new characters in the orchid .The purpose of this research was to discover the impact of gamma ray irradiation in vitro to the diversity of D. sylvanum. The doses of gamma ray used in this research were 0 Gy, 15 Gy, 30 Gy, 45 Gy, and 60 Gy. The results obtained indicated that mutation mostly occurred on the treatment with 15 Gy (4.4 cm of plant height, 2.67 of leaves, 2.36 cm of leaf length, 0.49 cm of leaf width, and 5.33 of root strands) compared to the other doses and the control plants. The new finding in this research is the gamma ray dose that can optimally stimulate the mutation in D. sylvanum. This information is useful to generate the new variety in orchid cultivation in Indonesia. This research provides innovation in orchid cultivation and new variety that is possible to arise after the mutation.
EFEK NAUNGAN DAN CEKAMAN AIR TERHADAP PERTUMBUHAN DAN HASIL KUNYIT DI KISMANTORO, WONOGIRI Ratri, Anindita Dwi Yogi Sapta; Pujiasmanto, Bambang; Yunus, Ahmad
Caraka Tani: Journal of Sustainable Agriculture Vol 30, No 1 (2015): March
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (256.782 KB) | DOI: 10.20961/carakatani.v30i1.11826

Abstract

Turmeric is a medicinal plant that has largest secondary metabolites (curcumin). Improving secondary metabolites with abiotic stress that the provision of shade and water stress. This research aims to study the effects of shade and water stress on growth and yield of turmeric and content of secondary metabolites. The study uses a nested design with two factors, namely shade (without shade, 25%, 50%, 75%) and water stress (without stress, 25% field capacity, 50% field capacity, 25% field capacity). Analysis using the F test and DMRT 5% level. The results showed that shade did not effect to fresh and dry weight of plant. 75% shade decrease root lenght, fresh and dry weight of rhizome. Water stress did not effect to growth and yield of turmeric. The highest curcumin results in conditions without shade and without stress.
Budidaya Organik Kunyit pada Kluster Biofarmaka Kabupaten Karanganyar Samanhudi, Samanhudi; Yunus, Ahmad; Pujiasmanto, Bambang
Caraka Tani: Journal of Sustainable Agriculture Vol 33, No 1 (2018): April
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v33i1.19112

Abstract

Potential development of medicinal plants in Karanganyar is very large, because this area is famous as a center of medicinal plants such as ginger, turmeric, kencur, and temulawak. In the development of medicinal plants, the approach of organic cultivation is one of the alternatives that can be done to achieve the business. Therefore the application of technology to society about organic farming on Biopharmaca Cluster of Karanganyar was conducted with the aim to apply organic cultivation techniques that have resulted from previous studies, so it can be applied by the community to increase income and welfare. This application of technology to society activity is held in Sambirejo, Jumantono, Karanganyar. The research was conducted from March to October 2016. The results showed that the application of chicken manure affects the number of leaves, number of tillers, and the fresh weight of turmeric rhizome. The addition of manure (chicken, goat, cow) can increase all variables of planting growth which include plant height, number of leaf, number of tillers, fresh weight of plant, dry weight of plant, and fresh weight of rhizomes. Giving three types of manure (chicken, goat, cow) with mycorrhizal dose treatment there is no intraction to some growth observation variables. Mycorrhizal treatment with a dose of 10 g/plant can increase the number of tillers in turmeric. Overall the application of mycorrhizal at various doses (5, 10, and 15 g/plant) can increase plant height, leaf number, number of tillers, and fresh weight of turmeric rhizome.
PENGARUH KONSENTRASI NAA DAN AIR KELAPA TERHADAP MULTIPLIKASI TEMULAWAK (CURCUMA XANTHORRIZHA ROXB.) SECARA IN VITRO Pranata, Moch. Galih; Yunus, Ahmad; Pujiasmanto, Bambang
Caraka Tani: Journal of Sustainable Agriculture Vol 30, No 2 (2015): October
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (261.485 KB) | DOI: 10.20961/carakatani.v30i2.11890

Abstract

Java turmeric is a medicinal plant which in top ranks in its uses. Many people choose herbal treatments for their health. This study aims to investigate the response of the multiplication of java turmeric against granting PGR NAA and young coconut water in vitro. This research was conducted at the Laboratory of Biotechnology and Tissue Culture, Sebelas Maret University, Surakarta. The research was arranged in a Fully Randomized Design with 16 treatments and 3 replications. The results showed that giving kombination treatment of 0,5 ppm NAA and 60% young coconut water was able to increase root amount with by an average of 20,33. NAA 1,5 ppm can increase buds amount at 2 buds. Giving 20% young coconut water treatment were also capable of increase average length buds, root length in sequence 7,37 cm and 7,37 cm.
Profile of Bottom Soil of Yellow Podzolic Red (YPR) with Different Ages in The Catfish (Pangasius sp.) Intensive culture Yunus, Ahmad; Hasibuan, Saberina; Syafriadiman, Syafriadiman
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 2 Juli s/d Desember 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ABSTRACT   This research was conducted from March to May 2017in the Koto Masjid Village, Kampar, Riau and observation parameters has done in the Environmental Quality Laboratory of Aquaculture Faculty of Fisheries and Marine University of Riau. The aim of research was to knowing the layers on the profile of bottom soil of yellow podzolic red (YPR) pond and its characteristic with different age which catfish cultivated (Pangasius sp.) intensively. This research used eksperiment method and had direct observation with 1 factor, 4 treatments and 3 replications. The highest basal soil profile of the initial layer of flocculent layer (F) was P1 (0-5 years age) of 0.98 cm while in F final was P3 (age of 11-15 years). Furthermore, in the highest mixed sediment layer (S) layer, P3 is 3.98 cm, whereas in the final S layer is P2 (aged pond 6-10 years) that is 7.91 cm. Furthermore, the highest stable matures is stable sediment (M) is P4 (16-20 years age pond) which is 8.59 cm, while in the final M layer is P2 which is 11,50 cm. The next stage of the highest transitional layer (T) layer is P3 which is 10.54 cm, whereas in the final T layer it is P2 that is 17.16 cm. The measured soil quality parameters are still considered good. Soil sand fraction 74,16-89,66%, silt fraction 2,66-9,83%, clay fraction 7,66-16,00%. Ground BV 0.45-1.58 g / cm3, ground BJ 1,53-2,18 g / ml, soil porosity 14,75-66,26%, C organic soil 1,97-5,60% and pH 6,80-6,96.Keywords: Bottom Soil Pond Profile, Flocculent layer, YellowPodsolik Red, Pangasius sp.
Design of stem separator tool with Cyclea Barbata (Cincau) plant leaves to increase business productivity of Sunter Jaya Residents of Jakarta Utara Yunus, Ahmad; Kurniaty, Ika; Sari, Fatma; Prasetyawati, Meri; Chamdareno, Prian Gagani; Kurnianto, Tri
ABDIMAS TALENTA: Jurnal Pengabdian Kepada Masyarakat Vol. 5 No. 2 (2020): ABDIMAS TALENTA : Jurnal Pengabdian Kepada Masyarakat
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (674.096 KB) | DOI: 10.32734/abdimastalenta.v5i2.4585

Abstract

Cincau (Cyclea barbata) is a plant whose leaves can be squeezed thick for the contents of a drink. This plant is a species of the cyclea genus. Green grass jelly leaves are found in various places in Indonesia, from traditional markets to modern shopping centers. This plant is known as camcao (Javanese), camcauh (Sundanese), juju, kepleng, krotok, tarawalu, tahulu (Malay). There are several types of grass jelly known today, namely green grass jelly, black grass jelly, and oil grass jelly. Indonesian people are fond of green grass jelly because the physical nature of green grass jelly leaves (Cyclea barbata) is thin and limp so that it is easier to form into gelatin or into gelatin. So far, research that examines quality improvement in the processing process has not been given much attention, because many think that the process currently carried out meets the standards. For this reason, through this research, efforts were made to improve the quality of Cyclea barbata leaves through the initial stages of the Cyclea barbata leaf processing process. The stalks and leaves of the henna must be separated from the beginning of the processing process before being processed further, so that the pure processed material comes from Cyclea barbata leaves which produce the best Cyclea barbata leaf powder, so that it is expected to produce quality Cyclea barbata leaf products. For that we need a separator for the leaves and stems of the Cyclea barbata plant. This research is needed to produce a design for separating the stem and leaves of the Cyclea barbata plant. The design of this tool needs to refer to the physical characteristics of the leaves and stems of the Cyclea barbata plant, so that power requirements are reduced and operational costs decrease.
Standardization of The Scale of Ethnocultural Empathy in Central Indonesia Region (Bali, Kalimantan and Sulawesi) Fitniwilis, Fitniwilis; Yunus, Ahmad
GUIDENA: Jurnal Ilmu Pendidikan, Psikologi, Bimbingan dan Konseling Vol 11, No 1 (2021)
Publisher : Universitas Muhammadiyah Metro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24127/gdn.v11i1.3556

Abstract

Indonesia is a multi-cultural country and has a plurality in many dimensions. This pluralistic culture is a nation's wealth capital in understanding and interacting with other nations. Current developments have led us to wider interactions across national borders where the relationship involves intercultural aspects. However, building and fostering ethnocultural empathy is an accumulative and continuous process and requires consistency over a long period of time. The most important first process is that we need to analyze the state of cultural empathy in society. This exploration, of course, requires a valid and reliable measuring instrument. As a challenge, we don't have limited instruments to explore this yet. In developing the instrument, the vast territory of Indonesia requires us to research in several periods to get maximum results. In this study, the Scale of Ethnocultural Empathy will be adapted and developed by Wang et al. and tested its validity and reliability in the central region of Indonesia. The number of items tested was 59 on 399 people, consisting of 18 males and 381 females. As for the distribution of the tribes living on Kalimantan, Bali, and Sulawesi, 24% came from the Javanese, and 44.86% of the total population came from the Makasarese, Bugis, Balinese, Ambonese, Minahasa, Dayak, and Kalimantan Malays. The study results showed that the scale of cultural empathy (The Scale of Ethnocultural Empathy / SEE) as a result of adaptation and development of 59 items was declared valid as many as 55 items and 4 items dropped. The results of the study also showed that who selected the scale of cultural empathy (The Scale of Ethnocultural Empathy / SEE) as many as 55 valid items, 58 items that met the item discriminatory value with riX 0.30 in the item discrepancy test stage, thus remaining 55 items based on the validity and item discrepancy. The results of the study also show that the scale of cultural empathy (The Scale ofEthnoculturall Empathy / SEE) as many as 55 items that meet the value of item discriminating power and validity was tested for reliability and obtained a Cronbach's Alpha score of 0.797 so that the instrument with 55 items can be accepted as a scale with reliability. Very high.