Christina Nugroho Ekowati, Christina Nugroho
Jurusan Biologi FMIPA Universitas Lampung, Bandar Lampung

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KARAKTERISASI ENZIM XILANASE DARI ISOLAT BACILLUS SP. UJ131 DI HUTAN MANGROVE MARGASARI LAMPUNG TIMUR SEBAGAI KANDIDAT PROBIOTIK Sumardi, Sumardi; Farisi, Salman; Ekowati, Christina Nugroho; Hairisah, Suminta Frida
OLDI (Oseanologi dan Limnologi di Indonesia) Vol 4, No 3 (2019)
Publisher : Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/oldi.2019.v4i3.201

Abstract

Characterization of Xilanase Enzyme by Bacillus sp. UJ131 A Probiotic Candidate from the Mangrove of Margasari, East Lampung. Mangrove are known to have high biodiversity as a potential location for exploration of enzyme-producing bacteria, one of which is the xylanase enzyme. Xylanase is an enzyme that hydrolyzes the xylan-rich hemicellulose substrate. This research aims to produce and know the character of xylanase enzyme by Bacillus sp. UJ131 isolate which was isolated from the oriental shrimp in Margasari mangrove forest of East Lampung. Xylanase enzymes are produced from the bacteria by growing them in sea water complete (SWC) broth containing xylan beechwood. Characterization xylanase enzyme consist of pH, temperature, and metal ions. The results showed that xylanase had an optimal time of 18 hours with xylanase activity of 0.18 U/ml, and optimally at pH 6 and temperature 70°C. The addition of Mn2+ and Ca2+ ions increase enzyme activity, but Fe3+, Cu2+ and Mg2+ ions decrease xylanase enzyme activity. At a maximum reaction speed of 0.25 U/ml, the xylanase enzyme can produce 1.30 ?M xylose per minute
PENGARUH PH TERHADAP PRODUKSI ANTIBAKTERI OLEH BAKTERI ASAM LAKTAT DARI USUS ITIK Sinaga, Edelina Sinaga; Ekowati, Christina Nugroho; Sutrisna, Rudy
Jurnal Penelitian Pertanian Terapan Vol 15, No 3 (2015)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jppt.v15i3.135

Abstract

This study aimed to determine the effect of pH on the production of antibacterial by lactic acid bacteria. This study uses a completely randomized design factorial 3X 5 (3 X 5 treatment of bacterial isolates pH) with three replications. The first factor consisted of pH 4, pH 5, pH 6, pH 7, pH 8, and the second factor that isolates B2, B7, B8, whereas the parameters consists of a clear zone. The amount of production is determined by the size of the diameter of antibacterial inhibition zone against Salmonella sp. Differences in the production of antibacterial determined based on the results of analysis of variance. The treatment significantly further test BNT at 5% level. The results showed that the effect of media pH test based on the potential for the production of antibacterial effect. The diameter of the largest clear zone produced by B7 isolates at pH 6 was 17.2 mm, 17.1 mm B8 and B2 at pH 7 at 16.7 mm. The smallest diameter of the clear zone produced by B8 at pH 4 was 15.1 mm, B2 at pH 6 there is a clear zone of 12.7 mm and isolate B7 at pH 5 of 11.5 mm Keywords: Antibacterial, Lactic Acid Bacteria, pH, Salmonella sp, Clear zone
Uji Daya Hidup Bakteri Asam Laktat Dari Usus Itik Pada Media Tumbuh Dengan Penambahan Variasi Konsentrasi Molasis Sutrisna, Rudy; Ekowati, Christina Nugroho; Damayanti, Riska
Jurnal Penelitian Pertanian Terapan Vol 16, No 1 (2016)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (220.422 KB) | DOI: 10.25181/jppt.v16i1.74

Abstract

This study aims to determine differences in the viability of Lactic Acid Bacteria (LAB) isolates on MRS medium supplemented with various concentrations molasses. Concentration variation molasis used as controls were 0%, 1%, 2% and 3%. Variation of time incubation on each of the media that is 0 hours, 24 hours, 48 hours and 72 hours. The viability of each isolate was viewed with the population of Log (cells/ml) which grow on the medium and the number of generations and time of generations. The results showed that the concentration molasses added to MRS affect the viability of LAB. Concentration of 1% makes LAB better viability than controls. Total population continues to increase at the end of the incubation with a yield of 1.17 generations in time 20.46 hours/generation. In contrast to controls, reduced population size at the end of the incubation so as not to produce a generation. LAB viability in addition molasses 2% and 3% live on 48 hours of incubation. This is indicated by the number of population increased at 48 hours of incubation, and then decreased at the end of incubation. The addition of concentration variation in the types of bacteria showed different viability. At B1 adding molasses 2% and 3% live on 48 hours of incubation and control can only live on a 24 hours incubation, whereas the B3, the control can remain alive at 48 hours of incubation. In contrast to the controls on B4 can survive on 48 hours of incubation, whereas the addition of 3% molasses can only live on a 24 hours incubation. Key words : Lactic Acid Bacteria/LAB, Molasses,  Viability
AKTIVITAS ENZIM HIDROLASE PADA PENAPISAN ISOLAT ACTINOMYCETES TOLERAN SALINITAS Sumardi, Sumardi; Qatrunada, Vanya; Farisi, Salman; Arifiyanto, Achmad; Ekowati, Christina Nugroho; Farisi, Salman
BIOMA Vol 6, No 1 (2021): BIOMA:JURNAL BIOLOGI DAN PEMBELAJARAN BIOLOGI
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/bioma.v6i1.3548

Abstract

Actinomycetesis one of the microorganisms which have the ability of enzymes hydrolases such as cellulose, amylase, protease, and manannase. Manufacturer of enzymes of the hydrolase can be used as a candidate probiotic. A candidate probiotic should have a good resistance in different environmental conditions including pH and salinity.  This research is conducted to filter the candidate’s probiotics from bacteria Actinomycetes that tolerance salinity and has the activity of the enzyme hydrolase. Testing the activity of the enzyme was preformed qualitatively on the variation of pH 4, 7, and 9.8.  Theability of Atinomycetesproduce enzymes hydrolases is indicated by the colony on media containing the substrate.From the test results, cellulase enzyme activity with the largest enzymatic index value is 3.5 AF2 isolates pH 9.8. Amylase enzyme activity with the largest enzymatic index value is 3.66 RH1 isolates pH 7. Protease enzyme activity with the largest enzyme index value is 0.66, AF2 isolates at pH 7. Mananase enzyme activity with the largest enzymatic index value is 0.85, namely AF2 isolates pH 4. In testing the effect of salinity test isolates were able to grow at variations in the concentration of NaCl 0, 3, and 6%.