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All Journal Folia Medica Indonesiana Jurnal Biosains Pascasarjana MPI (Media Pharmaceutica Indonesiana) Journal of Natural Sciences and Mathematics Research THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST PEDAGO BIOLOGI Jurnal Pembelajaran Pemberdayaan Masyarakat (JP2M) Humanism : Jurnal Pengabdian Masyarakat Indonesian Academia Health Sciences Journal KARYA: Jurnal Pengabdian Kepada Masyarakat Empowerment: Jurnal Pengabdian Masyarakat Mukhtabar: Journal of Medical Laboratory Technology
Yeti Eka Sispita Sari
Universitas Muhammadiyah Surabaya
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Journal : THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

 1 
Gambaran Histologi Ginjal Tikus Wistar Yang Terpapar MSG Setelah Perlakuan Diberikan Jus Tomat Dan Diberhentikan Perlakuan Saja Yeti Eka Sispitasari
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 1, No 2 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (501.639 KB) | DOI: 10.30651/jmlt.v1i2.1499

Abstract

MSG causes many side effects on the body, but MSG has long been used as a food flavor that can bring tastes (umami) and play a role in strengthening the taste. The chemical structure of MSG is no different from Glutamic Acid (glutamate), it is one of the 20 amino acids that make up proteins in the body. So the use of MSG needs to be discontinued to prevent kidney damage due to continued consumption of MSG, two research were conducted to determine the effect of discharging of MSG consumption by being given tomato juice (1) and discharged without treatment (2), the second study was seen from the histology picture of rats wistar mouse.Methods were experimental with post test only control group design. The research (1) used 15 wistar rats divided into three groups: group I without treatment (pellet AD II and drinking water), group II was given MSG for 14 days and group III was given tomato juice and MSG for 14 days. The study (2) used 27 rats divided by 9 groups. The sample is selected by simple random sampling method. The mice were then gradually turned off on the 29th, 43rd and 57th days. There was no significant difference in mean number of normal and damaged proximal tubules in all treatment groups. The renal histologic features in the treatment group I (MSG) and the treatment group II (MSG and tomato juice) showed normal glomeruli, tubular epithelial tubules, and lumen tubular narrowing whereas in mice discharged without treatment there was no apparent difference between the two MSG Giving groups damage to proximal tubules and renal corpusculum and regeneration after 14 days of discontinuation of MSG. Conclusions from these two experiments occurred damage to the renal tubules Keywords: MSG, Kidney, Wistar Rat
Efektivitas Perasan Daun Bunga Sepatu (Hibiscus rosa-sinensis L) Terhadap Pertumbuhan Staphylococcus aureus Yeti Eka Sispitasari
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 1, No 1 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (209.428 KB) | DOI: 10.30651/jmlt.v1i1.1011

Abstract

Resistensi antibiotik merupakan masalah yang dihadapi oleh tenaga medis di seluruh dunia. Menurut WHO angka kematian akibat resistensi antibiotik sampai tahun 2014 sebesar 700.000 per tahun. Salah satu faktor pemicu meningkatnya kasus yakni penggunaan antibiotik yang tidak bijak pada manusia. Diperlukan alternatif antibiotik untuk mengendalikan infeksi bakteri, yang murah dan aman dengan menggunakan bahan alami berasal dari tanaman. Salah satu alternatifnya adalah daun bunga sepatu (Hibiscus rosa-sinensis L). Dalam Daun bunga sepatu terkandung senyawa antimikroba yaitu: taraxeryl acetat, flavonoid, polifenol, dan saponin. Tujuan penelitian ini adalah untuk mengetahui efektivitas perasan daun bunga sepatu (Hibiscus rosa-sinensis L) terhadap pertumbuhan Staphylococcus aureus.Jenis penelitian bersifat eksperimental laboratorium dengan post-test design group with control secara in vitro. Metode yang digunakan adalah metode difusi Ring dengan kosentrasi bahan uji yang digunakan 100%, 75%, 50%, 25%, dan kontrol negatif serta positif (tetrasiklin). Populasi penelitian adalah Staphylococcus aureus ATCC 25923 berasal dari Balai Besar Laboratorium Kesehatan Surabaya. Uji statistik yang digunakan adalah annova dengan syarat data harus berdistribusi normal dan homogen dan dilanjutkan ke uji tukey HSD.Hasil analisis data uji annova menunjukkan bahwa perasan daun kembang sepatu (Hibiscus rosa-sinensis L) efektif menghambat pertumbuhan Staphylococcus aureus dengan (nilai ρ<0,05). Diameter zona hambat perasan terbesar pada kosentrasi 100% yakni 26,6 mm dan terkecil pada 25% yakni 7,6 mm.Berdasarkan hasil penelitian daun bunga sepatu (Hibiscus rosa-sinensis L) belum bisa dianggap sebagai alternatif untuk penanggulangan penggunaan antibiotik kimia dikarenakan kontrol pembanding yang gunakan merupakan golongan antibiotik generasi pertama. Sehingga perlu diadakan penelitian dengan menggunakan kontrol pembanding antibiotik generasi terbaru. Kata kunci : Daun Bunga sepatu, Staphylococcus aureus 
Co-Authors Ahmad Mochtar Jamil Ahmad Yudianto Ainutajriani, Ainutajriani Alfiansya Noval Siswanto Andre Ridho Saputro Anindita Riesti Retno Arimurti DITA ARTANTI Dita Artanti Ellies Tunjung Sari Maulidiyanti Fitrotin Azizah Fitrotin Azizah, Fitrotin Luluk Latifah Muhammad Anas Mulya Fitrah Juniawan Mustofa, Nabila Amelia Nastiti Kartikorini, Nastiti Nur Vita Purwaningsih, Nur Vita Rinza Rahmawati Samsudin Rohmayani, Vella Saputro, Tri Ade Vella Rohmayani