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All Journal ASEAN Journal of Chemical Engineering Makara Journal of Technology
Anondho Wijanarko
Department of Chemical Engineering, Faculty of Engineering, University of Indonesia, Depok 16424, Indonesia
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Electrical & Electronics Engineering Engineering Materials Science & Nanotechnology Mechanical Engineering

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The Initial Dioxigenase Gene Squences Analysis of Marine Bacteria Strain M128 Muhamad Sahlan; Hanif Yuliani; Heri Hermansyah; Anondho Wijanarko
ASEAN Journal of Chemical Engineering Vol 13, No 1 (2013)
Publisher : Department of Chemical Engineering, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (953.401 KB) | DOI: 10.22146/ajche.49723

Abstract

Biodegradation of polyaromatic hydrocarbons (PAHs) are catalyzed by multicomponent enzymes from microbe. The initial dioxygenase was used as a key enzyme for attacking the aromatic ring structure of PAHs, furthermore its initial dioxygenase gene was used to select PAHs degrading bacteria. Marine bacteria M128 strain could grow on medium contained PAHs. Detection of its cellular initial deoxygenase gene was done by nahAc gene amplification. The nahAc gene commonly used as biomarkers of PAH degradation, and as a result, nahAc gene sequence analysis of marine bacteria M128 strain was similar to naphthalene dioxygenase of Pseudomonas genera with 99% homology.
Effect of Photoperiodicity on Co2 Fixation By Chlorella vulgaris Buitenzorg in Bubble Column Photobioreactor For Food Supplement Production Wijanarko, Anondho; Dianursanti, Dianursanti; Witarto, Arief Budi; Soemantojo, Roekmijati Widaningroem
Makara Journal of Technology Vol. 8, No. 2
Publisher : UI Scholars Hub

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Abstract

To reduce the level of CO2 content in air, effort on converting CO2 to useful products is required. One of the alternatives includes CO2 fixation to produce biomass using Chlorella vulgaris Buitenzorg. Chlorella vulgaris Buitenzorg is applied for production of food supplement. Chlorella vulgaris Buitenzorg is also easy to handle due to its superior adaptation. Currently, Chlorella vulgaris Buitenzorg has been analyzed by some experts for its cellular composition, its ability to produce high quality biomass and the content of essential nutrition. A series of experiments was conducted by culturing Chlorella vulgaris Buitenzorg using Beneck medium in bubbling column photobioreactor. The main variation in this experiment was photoperiodicity, where growth of Chlorella vulgaris Buitenzorg was examined during photoperiodicity condition. The difference between CO2 gas concentration of inlet and outlet of the reactor during operational period, was compared to the same experiment under continuous illumination. Under photoperiodicity of 8 and 9 h/d, the culture cell densities (N) were approximately 40 % higher than under continuous illumination. Final biomass density of Chlorella vulgaris Buitenzorg at 9 h/d illumination was 1.43 g/dm3, around 46% higher than under continuous illumination. Specific carbon dioxide transfer rate (qCO2) in photoperiodicity was 50-80% higher than under continuous illumination. These experiments showed that photoperiodicity affects the growth of Chlorella vulgaris Buitenzorg The specific growth rate (μ) by photoperiodicity was higher than that by continuous ilumination while the growth period was two times longer. Based on the experiments, it can be concluded that photoperiodicity might save light energy consumption. The prediction of kinetic model under continuous illumination as well as under photoperiodicity illumination showed that Haldane model became the fitted kinetic model.
Utilization of Bagasse Cellulose for Ethanol Production through Simultaneous Saccharification and Fermentation by Xylanase Samsuri, M.; Gozan, Misri; Mardias, R.; Baiquni, M.; Hermansyah, Heri; Wijanarko, Anondho; Prasetya, Bambang; Nasikin, M.
Makara Journal of Technology Vol. 11, No. 1
Publisher : UI Scholars Hub

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Abstract

Utilization of Bagasse Cellulose for Ethanol Production through Simultaneous Saccharification and Fermentation by Xylanase. Bagasse is a solid residue from sugar cane process, which is not many use it for some product which have more added value. Bagasse, which is a lignosellulosic material, be able to be use for alternative energy resources like bioethanol or biogas. With renewable energy resources a crisis of energy in Republic of Indonesia could be solved, especially in oil and gas. This research has done the conversion of bagasse to bioethanol with xylanase enzyme. The result show that bagasse contains of 52,7% cellulose, 20% hemicelluloses, and 24,2% lignin. Xylanase enzyme and Saccharomyces cerevisiae was used to hydrolyse and fermentation in SSF process. Variation in this research use pH (4, 4,5, and 5), for increasing ethanol quantity, SSF process was done by added chloride acid (HCl) with concentration 0.5% and 1% (v/v) and also pre-treatment with white rot fungi such as Lentinus edodes (L.edodes) as long 4 weeks. The SSF process was done with 24, 48, 72, and 96 hour’s incubation time for fermentation. Variation of pH 4, 4,5, and 5 can produce ethanol with concentrations 2,357 g/L, 2,451 g/L, 2,709 g/L. The added chloride acid (HCl) with concentration 0.5% and 1% (v/v) and L. edodes can increase ethanol yield, The highest ethanol concentration with added chloride acid (HCl) concentration 0.5% and 1% consecutively is 2,967 g/L, 3,249 g/L. The highest ethanol concentration with pre-treatment by L. edodes is 3,202 g/L.
Biogasoline Production from Palm Oil Via Catalytic Hydrocracking over Gamma-Alumina Catalyst Wijanarko, Anondho; Mawardi, Dadi Ahmad; Nasikin, Mohammad
Makara Journal of Technology Vol. 10, No. 2
Publisher : UI Scholars Hub

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Abstract

Biogasoline Production from Palm Oil Via Catalytic Hydrocracking over Gamma-Alumina Catalyst. Bio gasoline conversion from palm oil is an alternative energy resources method which can be substituted fossil fuel base energy utilization. Previous research resulted that palm oil can be converted into hydrocarbon by catalytic cracking reaction with γ-alumina catalyst. In this research, catalytic cracking reaction of palm oil by γ-alumina catalyst is done in a stirrer batch reactor with the oil/catalyst weight ratio variation of 100:1, 75:1, and 50:1; at suhue variation of 260 to 340oC and reaction time variation of 1 to 2 hour. Post cracking reaction, bio gasoline yield could be obtained after 2 steps batch distillation. Physical property test result such as density and viscosity of this cracking reaction product and commercial gasoline tended a closed similarity. According to result of the cracking product’s density, viscosity and FTIR, it can conclude that optimum yield of the palm oil catalytic cracking reaction could be occurred when oil/catalyst weight ratio 100:1 at 340 oC in 1.5 hour and base on this bio gasoline’s FTIR, GC and GC-MS identification results, its hydrocarbons content was resembled to the commercial gasoline. This palm oil catalytic cracking reaction shown 11.8% (v/v) in yield and 28.0% (v/v) in conversion concern to feed palm oil base and produced a 61.0 octane number’s bio gasoline.
Biomass Production Chlorella Vulgaris Buitenzorg Using Series of Bubble Column Photo Bioreactor with a Periodic Illumination Wijanarko, Anondho; Dianursanti, Dianursanti; Muryanto, Muryanto; Simanjuntak, Josia; Kencana Wulan, Praswasti Pembangun Dyah; Hermansyah, Heri; Gozan, Misri; Soemantojo, Roekmijati Widaningroem
Makara Journal of Technology Vol. 12, No. 1
Publisher : UI Scholars Hub

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Abstract

Chlorella vulgaris Buitenzorg cultivation using three bubble column photo bioreactors arranged in series with a volume of 200 mL for 130 hours shows an increase of biomass production of Chlorella vulgaris Buitenzorg up to 1.20 times and a decrease of the ability of CO2 fixation compared to single reactor at a periodic sun illumination cycle. The operation conditions on cultivation are as following: T, 29.0oC; P,1 atm.; UG, 2.40 m/h; CO2, 10%; Benneck medium; and illumination source by Phillip Halogen Lamp 20W /12V/ 50Hz. Other research parameters such as microbial carbon dioxide transferred rate (qco2), CO2 transferred rate (CTR), energy consumption for cellular formation (Ex), and cultural bicarbonate species concentration [HCO3] also give better results on series of reactor.
Co-Authors Arief Budi Witarto Arief Witarto Arief Witarto, Arief Bambang Prasetya Bambang Prasetya Dadi Mawardi Dadi Mawardi, Dadi Dianursanti Dianursanti Dianursanti Dianursanti Dianursanti Dianursanti Hanif Yuliani Hanung Adi Nugroho Heri Hermansyah Josia Simanjuntak Josia Simanjuntak M Baiquni M Baiquni M Baiquni M Samsuri M Samsuri, M M. Baiquni Maemonah, Maemonah Mardias, R. Mawardi, Dadi Ahmad Misri Gozan Mohammad Nasikin Mohammad Nasikin Momoji Kubo Momoji Kubo, Momoji MUHAMAD SAHLAN Muryanto Muryanto Muryanto Muryanto, Muryanto Naomi Shibasaki-Kitakawa Naomi Shibasaki-Kitakawa, Naomi Nasikin, M. Praswasti P. D.K Wulan Praswasti P. D.K Wulan, Praswasti P. D.K Praswasti Pembangun Dyah Kencana Wulan Praswasti Wulan Praswasti Wulan, Praswasti R Mardias R Mardias, R Rita Arbianti Roekmijati Soemantojo Roekmijati Soemantojo Roekmijati Soemantojo, Roekmijati Roekmijati W. Soemantojo Roekmijati W. Soemantojo, Roekmijati W. Samsuri, M. Soemantojo, Roekmijati Widaningroem Tania Surya Utami Toshiy Yonemoto Toshiy Yonemoto, Toshiy Yuliusman Yuliusman