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Analysis AZF Gene Deletions in Infertile Men in Indonesia Hanizar, Evi; Hinting, Aucky
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Infertile men with the low quantity and quality of sperm associated with gene deletions in the long arm of chromosome Y (YQ), in the region known as the AZF region (Azoospermic Factor). This study aims to analyze the AZF gene deletions in infertile men with abnormal sperm categories ranging oligozoospermia, Oligoasthenoteratozoospermia until azoospermia. This study includes the type of cross-sectional observational study and DNA samples obtained from the blood of primary infertile men. Extraction of DNA uses a DNA extraction kit and DNA amplification uses polymerase chain reaction method. The analysis includes the number, motility and morphology of sperm conducted by WHO standard. Analysis of deletion is determined from the size of the base pairs of DNA amplification product. The results showed the prevalence of deletions in azoospermic category higher than the prevalence in other categories. Sequentially, the prevalence of deletions was followed by a severe category Oligoasthenoteratozoospermia, Oligoasthenoteratozoospermia, severe oligoteratozoospermia and oligoteratozoospermia. The most frequent gene deletions are sY86 gene, followed by sY84, while the most rare gene deletions are DAZ gene / sY255. This is in contrast with previous studies because of the background sample of infertile men from several races in Indonesia, the number of samples and the location of genes analyzed in the sub-region AZF. Deletions involving many genes in the AZF subregion associated with the smaller quantity and quality of sperm. Key Words : male infertility, azoospermic factor, gene deletion, sperm abnormality

THE EFFECT OF CONSUMING AVOCADO (Persea americana) ON MICE (Mus musculus) SPERM QUALITY Tiara Amelia, Yasinta; Hanizar, Evi; Nur Rikhma Sari, Dwi
BIOVALENTIA: Biological Research Journal Vol. 7 No. 1 (2021)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

The nutrients such as protein and vitamin are proven to improve the sperm quality. One nutrient rich fruit is avocado round green variety, which contains vitamins A, C and E higher than other varieties. This study aimed to determine the effect of consuming avocado on the mice sperm quality. This experimental study was using a Completely Randomized Design with four treatments, each with eight replications. Avocado dosage treatments were control, 75 % w/v, 100% w/v, and 133% w/v , with the number of mice were 32 age four weeks. Avocado was given three times a day, each 0.5 mL for six weeks besides the main food. The observed response was the sperm quality including number, motility and morphology. Sperm was taken from the epididymis after the mice were killed by cervical dislocation method. Sperm was made a suspension using 0.9% NaCl solution and a smear preparation to observe the sperm morphology while staining with basic stain crystal violet. The sperm quality was observed by Neubauer's counting rooms through a multimedia microscope. Data was analyzed using Kurskal Wallis test for the sperm number and Anova test for motility and morphological responses. The result showed that avocado had a significant influence for reproductive health, particularly for increasing spermatozoa quality, including concentration, motility and morphology of sperm. The higher dose of avocado given the higher quantity and quality of sperm resulted.

Aktivitas antibakteri Pleurotus ostreatus varietas Grey Oyster pada Staphylococcus aureus dan Pseudomonas aeruginosa Evi Hanizar; Dwi Nur Rikhma Sari
Pustaka Kesehatan Vol 6 No 3 (2018)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v6i3.9776

Oyster mushroom Pleurotus ostreatus varieties Gray oyster is often consumed by the community because it tastes delicious and contains nutrients for health but utilization for medical treatment has not been studied. The aim of this research was to analyze antimicrobial potential compounds in P. ostreatus and to investigate the inhibitory effect on the growth of Staphyllococcus aureus and Pseudomonas aeruginosa. This research was experimental type with treatment variation 0.1; 0.25; 0.5; 1; 1.5 and 2% of P. ostreatus extract, with 5 replicates. Antimicrobial potential compounds was analyzed by qualitative phytochemical test while antibacterial activity evaluated by calculating the inhibition zone diameter presented as the bright zone formed in the surroundings paper discs on bacterial cultures using solid medium. Phytochemical analysis obtained positive results for flavonoid, tannin and terpenoid compounds. The effect of mushroom extract on the bacterial growth inhibition were analyzed by One Way Anova test. The result showed that the inhibitory growth diameter for both bacteria was not significantly different. It meant that the difference in concentration variations of P. ostreatus extracts of 0.25 and 0.50 did not cause different inhibitory effects on S. aureus and P aeruginosa bacteria and 0.1% concentration was capable of causing inhibitory effect.

The Effect of Lemon (Citrus limon) Extracts On The Quantity and Quality of Mice (Mus musculus) Sperm Vitaloka Guci Rahayu; Evi Hanizar
Elkawnie: Journal of Islamic Science and Technology Vol 7, No 2 (2021)
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v7i2.9389

Abstract: Vitamin C has been proved as a nutrient to improve the quality of sperm. Society believed that consuming the lemons could potentially enhance the sperm quality of humans. However, the appropriate concentration should be well studied to obtain the optimum concentration to improve the sperm quality and quantity. The present research tried to provide information on how lemon could improve the sperm quality by designing a true experimental using a series concentration of lemon extract (25%, 50% and 75 % concentrations) given to the male mice (Mus musculus). The investigation was made by giving the lemon treatment three times a day for 5 weeks. To investigate the effect of lemon extract, the mice sperm were taken from the epididymis and observed using a multimedia microscope and counted using Neubauer’s counting rooms, while motility and morphology were observed using object-glass. The result showed that the high concentration of lemon could not provide the greatest improvement of sperm quality and quantity. The optimum condition was seen in 25% of lemon extract, where the increase of lemon concentration suppressed the lemon improvement effect, which reduced the sperm quality and quantity. However, the improvement was still made if the result was compared to control, meaning consuming lemon was better than consuming any lemon treatment. The result was in accordance with quality improvement of sperm, where 25% of lemon concentration treatment provided the highest average motility and normal morphology of sperm. However, the high concentration of lemon extract (50% and 75% of lemon concentrations) provided a lower effect due to the adverse effect. The result proved that lemon could be used to boost the quality and quantity of sperm in an appropriate concentration where the excess lemon extract could reduce the effect of lemon in improving sperm quality and quantity.Abstrak: Vitamin C telah terbukti sebagai nutrisi untuk meningkatkan kualitas sperma dimana masyarakat percaya bahwa mengkonsumsi lemon berpotensi meningkatkan kualitas sperma manusia. Namun, konsentrasi yang tepat harus dipelajari dengan baik untuk mendapatkan konsentrasi yang optimal untuk meningkatkan kualitas dan kuantitas sperma. Penelitian ini mencoba memberikan informasi bagaimana lemon dapat meningkatkan kualitas sperma dengan merancang eksperimen nyata menggunakan serangkaian konsentrasi ekstrak lemon (konsentrasi 25%, 50% dan 75%) yang diberikan kepada mencit jantan (Mus musculus). Penyelidikan dilakukan dengan memberikan ekstrak lemon sebanyak tiga kali dalam sehari selama 5 minggu. Untuk mengetahui pengaruh ekstrak lemon, sperma mencit diambil dari epididimis dan diamati menggunakan mikroskop multimedia dan dihitung menggunakan kamar hitung Neubauer, sedangkan motilitas dan morfologi diamati menggunakan kaca objek. Hasil penelitian menunjukkan bahwa konsentrasi lemon yang tinggi tidak dapat memberikan peningkatan kualitas dan kuantitas sperma yang tertinggi. Kondisi optimum terlihat pada ekstrak lemon 25% dimana peningkatan konsentrasi lemon menekan efek perbaikan lemon yang menurunkan kualitas dan kuantitas sperma. Namun perbaikan tetap dilakukan jika hasilnya dibandingkan dengan kontrol, artinya mengkonsumsi lemon lebih baik daripada tanpa mengkonsumsi lemon. Hasil tersebut sesuai dengan peningkatan kualitas sperma dimana perlakuan konsentrasi lemon 25% memberikan rata-rata motilitas dan morfologi sperma yang normal paling tinggi. Namun, konsentrasi tinggi ekstrak lemon (50% dan 75% konsentrasi lemon) memberikan efek yang lebih rendah karena efek samping. Hasil penelitian membuktikan bahwa lemon dapat digunakan untuk meningkatkan kualitas dan kuantitas sperma dalam konsentrasi yang sesuai dimana kelebihan ekstrak lemon dapat mengurangi efek lemon dalam meningkatkan kualitas dan kuantitas sperma.

DETEKSI FORMALIN PADA IKAN ASIN MENGGUNAKAN UBI JALAR UNGU (Ipomea batatas L.) Andrew Setyawan; Evi Hanizar
Saintifika Vol 23 No 2 (2021)
Publisher : Jurusan Pendidikan MIPA FKIP Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/saintifika.v23i2.28800

The content of formalin in salted fish needs to be known by the public in an easy and inexpensive way. Purple sweet potato (Ipomoea batatas) is one of the plants that contains anthocyanin which will show a color change when reacted with formalin. This study aims to determine whether purple sweet potato extract can detect formalin content in salted fish. The method used is a pure experiment with 5 treatments and 5 replications. Salted fish samples were made by the researcher himself, while purple sweet potato was extracted with a dose ratio of 1:1, 1:2 and 1:3 (v purple sweet potato extract /v aquadest). The observation parameter was the time when the color changed from purple to reddish in the salted fish sample after being dripped with purple sweet potato extract. The data were analyzed statistically using the Kurskall-Wallis test and continued with Duncan's test. The results of the analysis showed that purple sweet potato extract could detect the presence of formalin in salted fish samples (p < 0.05). The treatment with a dose of 1: 1 was able to detect the fastest formalin content at 735.40 seconds compared to treatment 1: 2 (915.20) and treatment 1: 3 (1038.40). This time is close to the time required by the positive control treatment (607.80). The conclusion of this study confirms that purple sweet potato can be used by the public to detect formalin content in food.

EFEK KONSUMSI KERANG BULU (Anadara antiquata) TERHADAP KUANTITAS DAN KUALITAS SPERMATOZOA Neny Safitri; Evi Hanizar
Al-Kauniyah: Jurnal Biologi Vol 12, No 2 (2019): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Kerang Bulu (Anadara antiquata) merupakan salah satu makanan yang mengandung nutrisi tinggi seperti protein, lemak, vitamin dan mineral yang berpotensi memperbaiki serta meningkatkan kualitas spermatozoa. Penelitian ini bertujuan untuk menganalisis efek kerang bulu (A.antiquata) terhadap kuantitas dan kualitas spermatozoa Mus musculus. Penelitian ini merupakan penelitian eksperimental murni dengan rancangan acak lengkap pola faktorial, faktor pertama kondisi kerang yakni mentah dan kukus, faktor kedua konsentrasi 0%(kontrol), 75% dan 100%. M. musculus yang digunakan sebanyak 25 ekor terdiri dari limAbstrakKerang bulu (Anadara antiquata) merupakan salah satu makanan yang mengandung nutrisi tinggi, seperti protein, lemak, vitamin, dan mineral, yang berpotensi memperbaiki serta meningkatkan kualitas spermatozoa. Penelitian ini bertujuan untuk menganalisis efek A. antiquata terhadap kuantitas dan kualitas spermatozoa Mus musculus. Penelitian ini merupakan penelitian eksperimental murni dengan rancangan acak lengkap pola faktorial. Faktor pertama adalah kondisi kerang, yakni mentah dan kukus, dan faktor kedua adalah konsentrasi 0 g/mL, 75 g/100 mL, dan 100 g/100 mL. M. musculus yang digunakan sebanyak 25 ekor, terdiri dari lima perlakuan dengan masing-masing lima kali ulangan. Parameter yang diamati adalah konsentrasi (kuantitas), motilitas, dan morfologi (kualitas) spermatozoa. Data dianalisis menggunakan uji Kruskal-Wallis dan uji lanjut Duncan. Hasil penelitian menunjukkan bahwa A. antiquata berpengaruh nyata terhadap konsentrasi dan motilitas spermatozoa, sedangkan morfologi tidak memberikan pengaruh yang nyata. Kondisi kerang mentah dengan konsentrasi 100 g/100 mL memberikan efek rata-rata terendah terhadap konsentrasi dan motilitas spermatozoa, sementara kerang kukus dengan konsentrasi 75 g/100 mL meningkatkan rata-rata konsentrasi dan motilitas spermatozoa tertinggi. Penelitian ini memberi kesimpulan bahwa mengonsumsi kerang kukus lebih baik untuk meningkatkan konsentrasi dan motilitas spermatozoa dibandingkan dalam kondisi mentah. Peningkatan dosis kerang kukus tidak linier terhadap respon kuantitas dan kualitas spermatozoa.Kata kunci: Anadara antiquata; Konsentrasi spermatozoa; Motilitas; Morfologi; Mus musculusAbstract Shellfish (Anadara antiquata) was one of the foods that contained high nutrients such as protein, fat, vitamins, and minerals that had the potential to improve and increase the quality of sperm. The study aims to analyze the effect of the shellfish on the quantity and quality of Mus musculus sperm. This study was a purely experimental study with a completely randomized factorial pattern; the first factor was the condition of shellfish which was raw and steamed, and the second factor was concentrations of 0 g/mL, 75 g/100 mL and 100 g/100 mL. Twenty-five individuals of M. musculus were subject to five treatments, each with five replications. Parameters observed were concentration, motility, and morphology of sperm. Data were analyzed using the Kruskal-Wallis test and Duncan's advanced test. The results showed that shellfish had a significant effect on the concentration and motility of sperm, whereas there was no significant effect on morphology. The dose which gave the effect was 100 g/100 mL of raw shellfish and 75 g/100 mL of steamed shellfish. This study concluded that consuming steamed shellfish was better than raw for increasing the concentration and motility of sperm. The shellfish dose increased is not linear in response to the quantity and quality of sperm.a perlakuan masing-masing lima kali ulangan. Parameter yang diamati jumlah (kuantitas), motilitas dan morfologi (kualitas) spermatozoa. Data dinalisis menggunakan uji Kruskal-wallis dan uji lanjut Duncan. Hasil penelitian menunjukkan kerang bulu (A.antiquata ) pengaruh nyata terhadap jumlah dan motilitas spermatozoa sedangkan pada morfologi tidak memberikan pengaruh yang nyata. Kondisi kerang mentah dengan konsentrasi 100g/100 ml air memberikan efek rata-rata terendah terhadap jumlah dan motilitas spermatozoa, sementara kerang kukus dengan konsentrasi 75g/100 ml meningkatkan rata-rata jumlah dan motilitas spermatozoa tertinggi. Penelitian ini memberi kesimpulan mengkonsumsi kerang kukus lebih baik untuk meningkatkan jumlah dan motilitas spermatozoa dibanding dalam kondisi mentah. Peningkatan dosis tidak linier terhadap respon kuantitas dan kualitas spermatozoa.

The Effect of Co-rearing with Females on the Quantity and Quality of Mouse (Mus Musculus) Spermatozoa Evi Hanizar; Titiek Fianti; Dwi Nur Rikhma Sari
BIOEDUKASI Vol 20 No 2 (2022)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v20i2.31849

Coexistence with the same sex is often discussed in recent times, but its effect on reproductive health has not been widely publicized. This study used male (Mus musculus) animals to determine the effect of co- rearing female animals on the quantity and quality of spermatozoa. The research type carried out was purely experimental with a completely randomized design with 4 treatments, namely control (P0) rearing which contained only 5 male mice, P1 rearing consisting of 3 males and 2 females, and P1 rearing consisting of 3 males and 2 females, P2 consisting of 2 males and 3 females while P3 contained 1 male and 4 female. Each treatment consisted of 6 replications. Animals are kept from 4 weeks old to 10 weeks old. Parameters observed are concentration, motility, and normal morphology of spermatozoa, and data analysis was tested by Kruskall-Wallis Test followed by Duncan’s test. the best spermatozoa compared to other treatments. The results showed that there were significant differences in the quantity and quality of spermatozoa, in the various treatment and maintenance groups, which indicated that the treatment with the composition of 1 male and 4 females had the best quantity and quality of spermatozoa compared to other treatments.

Pemanfaatan Taman Nasional Baluran sebagai Sumber Belajar Materi Keanekaragaman Hayati di Tingkat SMA Kelas X Evi Hanizar
BIO-CONS : Jurnal Biologi dan Konservasi Vol. 4 No. 1 (2022): BIO-CONS: Jurnal Biologi dan Konservasi
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31537/biocons.v4i1.1044

Kurangnya minat siswa untuk membaca disebabkan buku yang tebal dan kurang menarik. Oleh karena itu, perlu disediakan bahan bacaan yang menarik, mudah dibawa dan sesuai dengan tujuan pembelajaran yaitu dengan memanfaatkan lingkungan. Penelitian ini bertujuan untuk membuat media pembelajaran dengan menggunakan data-data yang diperoleh dari Savana Bekol di Taman Nasional Baluran. Jenis penelitian ini merupakan penelitian deskriptif dan pemilihan tempat ditentukan secara purposive karena mempunyai keanekaragaman hayati yang sesuai dengan tujuan pembelajaran di Sekolah Menengah Atas kelas X. Metode pengambilan data dilakukan dengan observasi secara langsung flora, fauna dan kondisi abiotik yang ada di savanna Bekol serta didukung dengan metode dokumentasi berupa foto. Data yang diperoleh dianalisis secara deskriptif menggunakan analisis taksonomi terhadap karakteristik flora dan fauna yang diperoleh. Hasil penelitian mendapatkan 9 spesies flora yaitu Brachiaria mutica, Chloris barbata, Dactyloctenium aegyptium, Eleusine Indica, Eragrostis amabilis, Eulalia amaura, Imperata cylindrical, Ischaemum muticum, Panicum repensum. Semua spesies tersebut termasuk dalam famili Poaceae. Sementara untuk fauna mendapatkan 11 species yaitu Bos javanicus, Bubalus bubalis, Callosciurus nigrovittatus, Cuon alpinus, Macaca fascicularis, Manis javanica, Muntiacus muntjak, Panthera pardus, Paradoxurus hermaphroditus, Cervus Timorensis dan Trachiypithecus auratus yang diklasifikasikan dalam famili Bovidae, Cervidae, Cercopithecoide, Sciuridae, Canidae, Felidae dan Viveridae. Berdasarkan hasil penelitian ini dibuat media booklet dan telah divalidasi oleh dua orang pendidik dan satu orang yang faham tentang media. Setelah dilakukan revisi dapat disimpulkan bahwa booklet ini layak digunakan sebagai media belajar untuk materi keanekaragaman hayati di tingkat SMA kelas X.

Aplikasi Pupuk Organik Cair (POC) Berbahan Dasar Urine Sapi pada Pertumbuhan Kunyit Putih Fatimatuz Zuhro; Titik Siti Rahma; Evi Hanizar
BIO-CONS : Jurnal Biologi dan Konservasi Vol. 5 No. 1 (2023): BIO-CONS: Jurnal Biologi dan Konservasi
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31537/biocons.v5i1.1090

Urine sapi merupakan limbah cair dari sistem pencernaan sapi, kaya dengan kandungan unsur hara dan hormon alami, sehingga dapat dimanfaatkan sebagai bahan dasar dalam pembuatan pupuk organik cair yang bagus bagi pertumbuhan tanaman budidaya. Penelitian ini bertujuan untuk mengetahui pengaruh aplikasi pupuk organik cair berbahan dasar urine sapi terhadap pertumbuhan kunyit putih (Curcuma zedoaria). Penelitian ini dilakukan dengan menggunakan Rancangan Acak Kelompok (RAK), terdiri dari 5 perlakuan konsentrasi POC urine sapi (0%, 5%, 10%, 20% dan 25%) dan 5 kali ulangan. Parameter pertumbuhan yang diamati antara lain; tinggi batang, jumlah daun, panjang daun, dan jumlah rimpang. Data hasil penelitian dianalisis dengan menggunakan Oneway Anova pada taraf signifikansi 95% dengan menggunakan uji lanjut Duncan. Hasil penelitian menunjukkan bahwa beberapa perlakuan konsentrasi POC urine sapi berpengaruh nyata terhadap semua parameter pengamatan.

Pengaruh Ekstrak Daun Bahagia (Dieffenbachia Sp.) terhadap Mortalitas Kecoa (Periplanetta Sp.) Hasni Ummul Hasanah; Evi Hanizar
BIO-CONS : Jurnal Biologi dan Konservasi Vol. 5 No. 1 (2023): BIO-CONS: Jurnal Biologi dan Konservasi
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31537/biocons.v5i1.1143

Kecoa (Periplanetta sp.) adalah salah satu serangga rumah tangga yang paling umum kita temukan di berbagai tempat. Kecoa juga menjadi vektor mekanis untuk beberapa penyakit seperti diare, keracunan makanan, tipus, disentri, polio, hepatitis a, dan cholera. Hal ini karena tubuh kecoa mampu mentransmisikan bakteri patogen seperti Salmonella sp., Staphylococcus sp., Streptococcus sp., Shigella sp. dan bakteri patogen lainnya. Cara pengendalian yang biasa digunakan selama ini adalah secara kimiawi menggunakan insektisida kimia yang memiliki dampak negatif terhadap manusia dan lingkungan. Penggunakan insektisida nabati diperlukan agar mengurangi kerusakan lingkungan yang diakibatkan oleh zat kimia. Daun bahagia (Dieffenbachia sp.) memiliki kandungan senyawa flavonoid dan minyak atsiri yang berkhasiat sebagai daya zat penolak (repellent) dan anti mikroba. Kandungan ekstrak daun Bahagia (Dieffenbachia sp.) dapat mengganggu sistem respirasi yang dapat mematikan kecoa (Periplanetta sp.). Kontrol positif menggunakan insektisida kimia dan ekstrak Daun Bahagia (Dieffenbachia sp.) menghasilkan jumlah mortalitas yang sama, akan tetapi pada menit ke lima, kontrol positif lebih cepat waktu mortalitasnya dibandingkan ekstrak daun Bahagia (Dieffenbachia sp.). Penggunaan daun Bahagia (Dieffenbachia sp.) berpotensi menjadi insektisida alami yang ramah lingkungan dan mudah didapatkan karena daun Bahagia (Dieffenbachia sp.) memiliki kandungan yang dapat membasmi serangga yaitu flavonoid dan minyak atsiri.

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