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All Journal Jurnal Penelitian Hutan dan Konservasi Alam Jurnal Wana Tropika
Maliyana Ulfa, Maliyana
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Agriculture, Biological Sciences & Forestry Environmental Science

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POPULASI FUNGI MIKORIZA ARBUSKULA (FMA) LOKAL PADA LAHAN PASCA TAMBANG BATUBARA Ulfa, Maliyana; Kurniawan, Agus; Sumardi, Sumardi; Sitepu, Irnayuli
Jurnal Penelitian Hutan dan Konservasi Alam Vol 8, No 3 (2011): Jurnal Penelitian Hutan dan Konservasi Alam
Publisher : Pusat Penelitian dan Pengembangan Hutan

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ABSTRAK Penelitian mengenai perkembangan jamur mikoriza arbuskula pada timbunan bukan tanah atasan dilakukan di areal bekas tambang batubara PT. Bukit Asam, Sumatera Selatan, pada 0, 8, 9, 10 dan 19 tahun pasca penimbunan. Tujuan penelitian adalah menganalisis keberadaan jamur mikoriza arbuskula serta hubungannya dengan umur lahan pasca penimbunan. Spora jamur mikoriza arbuskula diperoleh menggunakan metode saring basah. Hubungan keberadaan jamur mikoriza arbuskula dengan umur lahan pasca penimbunan dievaluasi menggunakan hubungan fungsional. Hasil penelitian menunjukkan bahwa Acaulospora sp. dan Gigaspora sp. ditemukan pada semua lokasi sedangkan Glomus sp. dijumpai setelah delapan tahun penimbunan. Ketiga jamur mikoriza menunjukkan karakter yang khas dalam kolonisasi di timbunan bukan tanah atasan, walaupun pada umur timbunan yang berbeda-beda. Penimbunan tanpa menggunakan top soil menyebabkan perkembangan jamur mikoriza arbuskula tidak berjalan dengan konsisten. Oleh karena itu, tanah bukan top soil sebaiknya tidak digunakan untuk menimbun areal bekas tambang batubara. 
IDENTIFIKASI MOLEKULER JENIS-JENIS JAMUR MIKORIZA EKTO YANG BERASOSIASI DENGAN DIPTEROKARPA DI HUTAN HUJAN TROPIKA SEKUNDER Ulfa, Maliyana; Faridah, Eny; Sumardi; Lee, Su See; Mansor, Patahayah; Roux, Christine le; Galiana, Antoine
Jurnal Wana Tropika Vol 9 No 1 (2019)
Publisher : Fakultas Kehutanan Institut Pertanian STIPER Yogyakarta

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Many types of soil born fungi associate mutually with high-level plant roots by forming mycorrhiza. While fruit body characterization was used previously, now molecular method wide used to identify ectomycorrhizal fungi when faces discontinuity fruit body presented on the floor and the important of identifying ectomycorrhizal fungi that lignning in particular association.Hence, the purpose of research is to identify the ectomycorrhizal fungi associated with dipterocarp species using moleculer method. Polymerase Chain Reaction (PCR) technique was conducted on DNA extract samples from root tips of dipterocarp trees and 3 (three) Shorea (S. leprosula, S. stenoptera, and S. mecistopteryx) seedlings intentionally planted under mature trees. DNA sequences were amplified using a specific primer pair of fungus and basidiomycetes ITS 1F-ITS 4B. The identity of ectomycorrhizal fungi was obtained by matching DNA sequence of the samples to Genbank database. Molecular identification resulted in 73 genotypes that belong to 13 families, i.e Thelephoraceae, Russulaceae, Clavulinaceae, Sebacinaceae, Inocyabaceae, Amanitaceae, Entolomataceae, Heliotialetaceae, Boletaceae, Cantharellales, Hymenogastraceae, Ceratobasidiceae, and Tricholomataceae. Thelephoraceae consists of 54 genotypes, Russulaceae and Sebacinaceae 13 genotypes, Clavulinaceae 6 genotypes, and the rest consist of 1-3 genotypes. Thelephoraceae is the dominant family in ectomycorrhizal jamurcommunities. Based on the results, it can be concluded that molecular method can be used to identify the real symbiont in mycorrhiza association. In addition, molecular methods can also detect the similarity of ectomycorrhizal fungi that colonizing dipterocarps, both at trees and seedlings level. Keywords :Ectomycorrhizal fungi, Dipterocarp species, Molecular technique, Secondary tropical rain forest
Co-Authors Agus Kurniawan Eny Faridah Galiana, Antoine Irnayuli Sitepu, Irnayuli Lee, Su See Mansor, Patahayah Roux, Christine le SUMARDI Sumardi .