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All Journal Jurnal Ilmiah Farmasi Pharmaciana: Jurnal Kefarmasian Seminar Nasional Hasil Riset dan Pengabdian (SNHRP) Jurnal Sains dan Kesehatan Jurnal Kesehatan Farmasi Borneo Journal of Pharmacy Medical Sains : Jurnal Ilmiah Kefarmasian
Sri Mulyaningsih
Program Studi Farmasi, Fakultas Farmasi, Universitas Ahmad Dahlan, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Other

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Journal : Jurnal Ilmiah Farmasi

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Uji Kepekaan Bakteri Escherichia Coli Hasil Isolasi Dari Urin Pasien Rumah Sakit Dr. Sardjito Terhadap Antibiotik Golongan P-Laktam Sri Harnanik; Sri Mulyaningsih; Asih Triastuti
Jurnal Ilmiah Farmasi Vol. 2 No. 1 (2005)
Publisher : Universitas Islam Indonesia

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Abstract

Uncontrolled use of antibiotic caused the emergence of bacteria strain resistant toward of antibiotic. The sensitivity assay of E. coli toward p-lactam antibiotic has been done. The sensitivity assay was started with isolated bacteria from patients urine at Dr. Sardjito hospital followed by count total of colony/number of germ which growth and identified the bacteria. The Kirby Bauer method was performed. Antibiotic impregnated disk (ampicillin 30 ng, sulbactam/ampicillin 20 ng, cefotaxim 30 ng, ceftazidime 30 jig, ceftriaxon 30 ng, cefpirom 30 ng, cefepim 30 ng and imipenem 10 ng) were placed on agar plate previously streaked with suspension of E. coli (1.10 CFU/ml). The plates were incubated for 18-24 hours at 37° C. The diameters of the zone inhibition were measured, and compared to standar interpretive zone sizes. The result of the study showed that all of E. coli resistant toward antibiotic ampicillin; 65% resistant toward antibiotic sulbactam/ampicillin; 50% still sensitive toward antibiotic third cefalosporin generation that is cefotaxim, ceftazidime, ceftriaxon; more than 50% sensitive toward fourth cefalosporin generation like cefepim, cefpirom and all of pathogen bacteria E. coli sensitive toward antibiotic imipenem.
Co-Authors Agustriyanata Vieri Ansharullah Arya Guna Ramadhan Asih Triastuti Dwi Utami Febriyati Adji Rachmadani Girly Risma Firsty Hendy Ristiono Iis Wahyuningsih Moch Saiful Bachri Muhammad Sa’id Nugroho Nadia Istiqomah Nining Sugihartini Reski Mulia Saputri, Ginanjar Zukhruf Sri harnanik Suparman Susan Fitria Candradewi Widyasari Putranti