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All Journal Jurnal Kebidanan Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya The Journal of Experimental Life Sciences Medical Journal of Indonesia Indonesian Journal of Tropical and Infectious Disease Majalah Kesehatan FKUB Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Asian Journal of Health Research
Tri Yudani Mardining Raras
(Scopus ID 56006496700) Department Of Biochemistry And Molecular Biology, Universitas Brawijaya, Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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Prolonged-heated High-Fat Diet Increase the Serum LDL Cholesterol Level and Induce the Early Atherosclerotic Plaque Development in Wistar Rats Yurina, Valentina; Yunita, Ema Pristi; Raras, Tri Yudani Mardining; Rudijanto, Achmad; Handono, Kusworini
Journal of Tropical Life Science Vol 9, No 1 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Rats are one of the most widely used animal models in health research. However, since rats are relatively resistant to atherosclerosis, the transgenic rats often used to study atherosclerosis in rats. Our study suggests a prolonged-heated lipid to induce atherosclerosis in rats, therefore provide a more low-cost option to study atherosclerosis in rats. Aims of this study is to compare the effect of heated high-fat diet (HFD) to the LDL and HDL cholesterol level and foam cell formation in the Wistar rat animal model. Rats were divided into three groups, control group was given with the normal diet, and the other two treated groups received oxidized HFD (heated HFD) and HFD, respectively. The heated HFD contain lard that was previously heated at 190°C for 24 h. Diet was given for 8 weeks. The serum LDL and HDL cholesterol level were measured before and 4th and 8th weeks after treatment with help of colorimetric measurement. The aorta analyzed for the foam cell formation after HE staining using the light microscope.  The prolonged- heated HFD significantly increased the LDL cholesterol from the 4th week of the treatment (p = 0.023). The rats aortic from HFD and heated HFD treated groups showed a mononuclear cells infiltration and the early foam cell formation. The heating process of the lipid caused lipid oxidation which significantly increased the LDL cholesterol level of the animal model and foam cells formation. This study suggests that Wistar rats with heated HFD could demonstrated early atherosclerosis plaque development.
Ekspresi IFN-γ dan IL-4 CD4+T Limfosit pada Tuberkulosis Kontak terhadap Antigen 38 Kda Mycobacterium tuberculosis Nugrahani, Iin Trilistiyanti; Kusuma, HMS Chandra; Raras, Tri Yudani Mardining; Arthamin, Maimun Zulhaidah; Astuti, Tri Wahju; Tanoerahardjo, Francisca
Jurnal Kedokteran Brawijaya Vol 28, No 4 (2015)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2015.028.04.8

Abstract

Mengendalikan epidemi tuberkulosis pada anak adalah prioritas kesehatan global. Efikasi vaksin Bacillus of Calmette and Guerin (BCG) sangat bervariasi sehingga memerlukan pengembangan vaksin baru. Antigen rekombinan 38 Kda Mycobacterium tuberculosis sebagai kandidat vaksin harus melalui uji imunogenitas. Tujuan studi ini untuk mengidentfikasi apakah antigen  38 Kda dapat menstimuli ekspresi IFN-γ dan IL-4 limfosit TCD4+ pada kultur PBMC anak dengan kontak TB. Studi ini dilakukan pada kultur PBMC dari 8 kontak TB dan 8 anak sehat yang diinduksi oleh antigen 38 Kda (kelompok 1), PPD (kelompok 2) dan tanpa perlakuan (kelompok 3). Ekspresi IFN-γ dan IL-4 limfosit TCD4+ diukur dengan flowcytometry. Rerata kadar IFN-γ untuk kontak TB tertinggi pada kelompok 3 (p=0,76), sedangkan rerata IL-4 tertinggi pada kelompok 2(p=0,68).RerataIFN-γ untukkelompok sehattertinggi pada kelompok3(p=0,78) sedangkanrerata IL-4 tertinggi pada kelompok 2 (p=0,32). Rerata ekspresi IFN-γ dan IL-4 yang diinduksi oleh antigen 38 Kda, masing-masing lebih tinggi pada kontakTB daripada subjek sehat (p=0,62 dan 0,39). Pengaruh respon imun yang protektif ditunjukkan oleh rasio ekspresi IFN-γ dan IL-4 yang lebih dari 1, baik pada kontak TB dan sehat (1,22 dan 1,28). Tidak ada perbedaan signifikan antara perlakuan pemberian antigen38 kDa, PPD dan tanpa perlakuan. Dapat disimpulkan bahwa antigen 38 Kda dapat menstimuli  ekspresi IFN-γ dan IL-4 limfosit TCD4+pada kultur PBMC kontak TB. Kata Kunci: Antigen rekombinan 38 Kda, Mycobacterium tuberculosis, IFN-γ, IL-4, limfositTCD4+
Biochemical study of CymJ, the putative regulator of cyclodextrin metabolism in Klebsiella oxytoca M5a1 Boeck, August; Raras, Tri Y.M.
Medical Journal of Indonesia Vol 16, No 2 (2007): April-June
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.338 KB) | DOI: 10.13181/mji.v16i2.258

Abstract

The products of ten genes clustered in two divergently oriented operons are required for the metabolism of cyclic oligoglucosides, the cyclodextrins, by Klebsiella oxytoca. The function of CymJ, the product of the promoter distal gene in one of the operons was studied. Over expression of cymJ in K. oxytoca led to strong reduction of the expression of the cym operons. This repression could be alleviated by addition of high concentration of α-cyclodextrin into the medium. There is a possible relationship between CymJ and CymD since the absence of CymD in a CymD deletion mutant prevented the repression effect of CymJ. An intriguing finding was that the presence of CymJ in large amount in the cell caused severe cell division inhibition leading to retardation of growth. This morphological change was paralleled by a significant increase in the susceptibility of K. oxytoca to ampicillin. (Med J Indones 2007; 16:69-77)Keywords: cym operon, putative regulator, over expression
Cloning and expression of pab gene of M. tuberculosis isolated from pulmonary TB patient in E.coli DH5α Lyrawati, Diana; M. Raras, Tri Y.
Medical Journal of Indonesia Vol 20, No 4 (2011): November
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4108.055 KB) | DOI: 10.13181/mji.v20i4.458

Abstract

Background: Mycobacterium tuberculosis antigen38 is a potent serodiagnostic agent containing two M. tuberculosisspecific B-cell epitopes. The high price of imported diagnostic agents hinders realization of fast clinical TB diagnosis in developing countries. Therefore, we produced recombinant antigen38 (recAg38M) from M. tuberculosis local strain, which might be used to produce economical tuberculosis serodiagnostic kit.Methods: Pab gene that was isolated from pulmonary TB patient in Malang was cloned into a plasmid vector (pGEMTeasy) to construct pMB38. The E.coli DH5α clone carrying pMb38 was selected on X-gal medium. The expression of pab was mediated using pPRoExHTc under the control of Trc promoter and E.coli DH5α as host.Results: Alignment of the pab sequence from the white E.coli DH5α clones with that of M. tuberculosis H37Rv showed 98% homology. The recombinant protein in which the signal peptide has been deleted to prevent the protein being secreted into medium was found in the cytoplasm.Conclusion: pab gene of M. tuberculosis isolated from a TB patient could be expressed in heterologous system in E.coliDH5α. (Med J Indones 2011; 20:247-54)Keywords: Mycobacterium tuberculosis, Pab gene expression, recombinant antigen38
PROFIL KADAR SOLUBLE UROKINASE PLASMINOGEN ACTIVATOR RECEPTOR (suPAR) PADA SERUM PENDERITA TUBERKULOSIS PARU (SEBAGAI MONITORING TERAPI) Astuti, Triwahju; MR, Tri Yudani; Riawan, Wibi; Muktiati, Nunuk Sri; Widjajanto, Edy
Jurnal Kedokteran Brawijaya Vol 24, No 1 (2008)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (136.873 KB) | DOI: 10.21776/ub.jkb.2008.024.01.4

Abstract

Dutch study shows that Upar expression content is significantly higher in tubercolusis patient’s psiphsy monosit compared to those in the healthy control group. So far, there is no biologic marker used whichcan accurately observe response improvement in the treatment of lung tubercolusis. The aim of this research is to investigate whether the serum level of soluble utokinase plasminogen activator receptor (suPAR) carries information in monitoring TB treatment for Lung Tuberculosis patients. suPAR was measured by ELISA in 21 individuals at the time of enrolment into observational cross sectional based on active tuberculosis  and 5 individuals as healthy control. There were 3 groups, 1). patients who had not started treatment (n=7),2). patients who had been treated for 2 months (n=7),  3). patients who had been treated for 6 months (n=7). Among groups, there were no difference in mean of body mass index, erythrocyte sedimentation rate and monocyte count. Patients positive for TB on direct  microscopy were 29% ( 6 from 21 patients) , 2 patients each groups. The suPAR levels were elevated in patients with active TB compared to healthy control (P<0,001). suPAR levels were highest in patients positive for TB on direct microscopy ( mean suPAR 4.455 ng/ml).
Potensi Kadar Neopterin sebagai Biomarker untuk Monitoring Kemajuan Terapi TB Paru pada Anak Sholeh, Aunillah Hamid; Raras, TY Mardining; Kusuma, HMS Chandra
Jurnal Kedokteran Brawijaya Vol 27, No 4 (2013)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2013.027.04.7

Abstract

Kadar neopterin plasma ditemukan berhubungan dengan efikasi terapi pada tuberkulosis paru pasien dewasa. Penanda biologis untuk diagnosis tuberkulosis pada anak masih terus diteliti. Penelitian ini dilakukan untuk mengidentifikasi peran neopterin sebagai penanda biologis untuk mengevaluasi keberhasilan terapi standar tuberkulosis paru pada anak. Sebanyak 25 pasien dengan tuberkulosis paru diikuti selama enam bukan. Kadar neopterin plasma diukur dengan metode ELISA sebelum terapi, 2, 4, dan 6 bulan sesudah terapi. Sepuluh anak sehat ditetapkan sebagai kontrol. Hasil menunjukkan pada semua pasien kadar neopterin tertinggi didapatkan sebelum terapi dengan rerata 0,27 (SD 0,09c ) ng/mL. Kadar neopterin plasma pada kelompok kontrol  1,50 (SD 0,18) ng/mL, sebelum terapi 1,12 (SD 0,22) ng/mL, bulan kedua 1,08 (SD 0,22) ng/mL, bulan keempat 0,97 (SD 0,35) ng/mL, dan bulan keenam 0,45 (SD 0,29) ng/mL. Kadar neopterin tertinggi didapatkan sebelum terapi yang kemudian menurun secara bertahap sesuai terapi bersamaan dengan perbaikan gejala klinis. Kadar neopterin pada kontrol lebih tinggi dibandingkan pasien selama masa pengamatan. Dapat disimpulkan kadar neopterin plasma mempunyai potensi sebagai biomarker kemajuan terapi tuberkulosis paru anak.Kata Kunci: Anak, biomarker, neopterin plasma, terapi, tuberkulosis paru
Efek Pemberian Protein Rekombinan Fusi ESAT6-CFP10 Mycobacterium tuberculosis terhadap Persentase IL2 dan IL10 yang Dipresentasikan Sel T CD8 pada Kultur PBMC Christianto, David; Raras, Tri Yudani Mardining; Sumarno, Sumarno; Arthamin, Maimun Zulhaidah; Astuti, Triwahju; Sardjono, Teguh Wahyu; S, Noorhamdani A
Jurnal Kedokteran Brawijaya Vol 30, No 3 (2019)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2019.030.03.7

Abstract

Keberhasilan vaksin BCG dalam memberikan perlindungan terhadap tuberkulosis (TB) pada orang dewasa di Indonesia belum optimal (37%) sehingga diperlukan vaksin alternatif yang lebih efektif. Protein rekombinan fusi ESAT6-CFP10 merupakan kandidat vaksin yang potensial. Penelitian dilakukan untuk menguji efektifitas protein rekombinan fusi ESAT6-CFP10 dalam meningkatkan ekspresi IL2 dan IL10 sel T CD8 yang memainkan peran penting dalam respon imun melawan TB. Pengujian kandidat vaksin dilakukan secara in vitro pada peripheral blood mononuclear cell (PBMC) dari kelompok sehat endemik TB, kelompok kontak TB, dan kelompok pasien TB dengan melihat persentase IL2 dan IL10 CD8. Setiap kelompok diberi perlakuan tanpa antigen, PPD, dan protein rekombinan fusi ESAT6-CFP10. Persentase IL2 meningkat secara signifikan dari kelompok sehat, kontak TB, hingga Pasien TB. Sebaliknya peningkatan persentase IL2 antar kelompok yang dipaparkan PPD tidak signifikan secara statistik (p=0,396). Persentase IL10 tidak menunjukkan perbedaan yang signifikan antar kelompoknya baik tanpa paparan antigen (p=0,617), PPD (p=0,351), maupun protein rekombinan fusi ESAT6-CFP10 (p=0,257). Didapatkan persentase IL2 yang tidak berbeda secara signifikan antar perlakuan pada kelompok sehat (p=0,309), kelompok kontak TB (p=0,318), dan kelompok pasien TB (p=0,424). Demikian juga dengan persentase IL10 yang tidak berbeda secara signifikan antar perlakuan pada kelompok sehat (p=0,908), kelompok kontak TB (p=0,352), dan kelompok pasien TB (p=0,776). Hal ini menunjukkan bahwa protein fusi rekombinan ESAT6-CFP10 dapat meningkatkan persentase IL2 tetapi tidak dengan IL10 meskipun secara statistik tidak signifikan.
Tinjauan Literatur: KEFIR: MIKROBIOLOGI, SENYAWA BIOAKTIF, DAN MANFAATNYA PADA PENYAKIT NONINFEKSI Tri Yudani Mardining Raras
Majalah Kesehatan Vol. 9 No. 4 (2022): Majalah Kesehatan
Publisher : Faculty of Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/majalahkesehatan.2022.009.04.7

Abstract

  Kefir semakin populer sebagai produk makanan fermentasi. Secara makroskopis butiran kefir memiliki struktur warna yang ireguler, multilobular, lengket dan berwarna kekuningan. Simbiosis mikrobiota antara bakteri dan khamir merupakan komponen utama dalam biji kefir. Bakteri yang paling banyak ditemukan pada butir kefir adalah Lactobaccilus sp. dan Lactococcus sp. sedangkan khamirnya didominasi oleh Saccharomyces sp. dan Kluveromyces sp. Kefir mengandung senyawa bioaktif seperti eksopolisakarida, bioaktif peptida, bacteriocin dan biosurfaktan yang berpotensi meningkatkan manfaat kesehatan dari kefir. Tinjauan literatur ini bertujuan untuk mengulas kefir dari aspek mikrobiologi, kandungan senyawa bioaktif kefir dan pengaruhnya sebagai agen imunomodulator atau imunostimulan pada artritis, antialergi, antikolesterol dan penyakit noninfeksi lain. Kefir berpotensi untuk dikembangkan sebagai produk kesehatan dan nutraseutikal sesuai dengan pengaruhnya terhadap stimulasi respons imun, antiartritis, aktivasi imunitas mukosa intestinal, perlindungan dari ulkus peptikum, antikanker, antimutagenesis, antialergi, menurunkan kadar kolesterol, mengurangi obesitas, dan antihipertensi. Oleh karena itu, kefir sangat potensial untuk dieksplorasi lebih lanjut dalam penelitian translasional sebagai salah satu bahan aditif dalam produk pangan fungsional yang memberikan manfaat untuk kesehatan.  
PROTEIN REKOMBINAN 38 KDA MYCOBACTERIUM TUBERCULOSIS DALAM INTERLEUKIN-2 DAN INTERLEUKIN-4 SERTA LIMFOSIT T CD3+ (The Mycobacterium Tuberculosis 38 kDa Recombinant Protein in Interleukin-2 and Interleukin-4 as well as CD3+ T Lymphocytes) Maimun Z Arthamin; Nunuk S Muktiati; Triwahju Astuti; Tri Yudani M Raras; Didit T Setyo Budi; Francisca S. Tanoerahardjo
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 3 (2015)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i3.1275

Abstract

Tuberculosis remains a serious global health problem despite the widespread use of the vaccine against tuberculosis (TB). Up tonow, the only available TB vaccine, Mycobacterium bovis BCG has a very wide efficacy range from 0 until 80 percent protection sothe development of a new vaccine is needed. The new protein as a candidate vaccine should be assessed for their immunogenicity. Thepurpose of this study was to examine whether Mycobacterium tuberculosis 38 kDa recombinant protein could stimulate a cellularimmune response especially CD3+T lymphocytes to express IL-2 and IL-4 in PBMC cultures. An experimental laboratory research oncultured PBMC of 3 groups consisting of TB patients, contacts of TB positive and healthy subjects, each group consisted of 8 subjects. AllPBMC cultures were induced by Mycobacterium tuberculosis 38 kDa recombinant protein, Purified Protein Derivative (PPD) and withoutantigen as a control. Expression of IL-2 and IL-4 CD3+ T lymphocytes was measured with flowcytometry. In healthy volunteers and TBcontacts there was a significant difference in the expression of IL-2 and IL-4 CD3+ T lymphocytes compared with no any treatment. Thehighest IL-2 expression was in healthy subjects [8.13 (0.622)] while the highest expression of IL-4 was in TB patients [6.436 (4.586)].Mycobacterium tuberculosis 38 kDa recombinant protein could induce the expression of IL-2 and IL-4 of CD3+ T lymphocytes in healthysubjects, TB contacts and TB patients and there were a significance differences in the expression of all groups.
PROTEIN REKOMBINAN 38 KDA MYCOBAKTERIUM TUBERKULOSIS DAPAT MENGIMBAS PEMBUATAN INTERLEUKIN-2 DAN INTERFERON-γ LIMFOSIT T DI KULTUR SEL MONONUKLEAR DARAH TEPI Maimun Z Arthamin; Singgih Pujo Wahono; Antiek Primardianti; Ati Rastini; Tri Wahju Astuti; Tri Yudani Mardining Raras; Francisca S Tanoerahardjo
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 2 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v22i2.1119

Abstract

Tuberculosis (TB) is caused by Mycobacterium tuberculosis (M.tb) and is one of the significant mortality causes WHO (2012). Theprimary immune response in TB pathogenesis is Cell Mediated Immunity (CMI), roled by T lymphocytes. Interleukin-2 (IL-2) is a growthfactor for T lymphocytes. Gamma Interferon is the key cytokine in M.tb infection control, synthezised by T lymphocytes. An effectivevaccination strategy is achieved by giving vaccine which is able to stimulate T lymphocytes in synthezising cytokines. The 38 kDa M.tbprotein is potential in the vaccine development program, because it has specific epitopes for T lymphocytes. The aim of this study was toknow how to determine that the 38 kDa recombinant protein of M.tb Malang strain could induce cellular immune response by IL-2 andIFN-γ synthezised by T lymphocytes. The study was carried out by an experimental in vitro study on PBMC from healthy endemic subjects,those having TB contact, and the TB patients themselves. PBMC from subjects was cultured with 38 kDa recombinant protein of M.tbMalang strain, with PPD and without any protein. The analysis of IL-2 and IFN-γ used flowcytometry. The result showed that the highestpercentage of IL-2 was found in the culture with 38 kDa recombinant protein of M.tb Malang strain, in healthy endemic (p=0.000)and in those who had TB contact (p=0.000). the highest percentage of IFN-γ was found in the culture with 38 kDa recombinant proteinof M.tb Malang strain, in healthy endemic (p=0.007) and those who had TB contact (p = 0.105). The 38 kDa recombinant proteinof M.tb Malang strain was able to induce IL-2 and IFN-γ synthezised by TCD3+ lymphocytes from healthy endemic subjects and thosewho had TB contact.
Co-Authors Achmad Rudijanto Achmad Rudijanto Antiek Primardianti Ati Rastini August Boeck Aunillah Hamid Sholeh David Christianto Diana Lyrawati Didit T Setyo Budi Dwi Yuni Nur Hidayati Edy Widjajanto Ema Pristi Yunita Ema Pristi Yunita, Ema Pristi Eviana Norahmawati Francisca S Tanoerahardjo Francisca S. Tanoerahardjo Francisca Tanoerahardjo, Francisca Galih Puspita Citra Mahardhika Gatoet Ismanoe Hani Susiati Herdian Fitria Widyanto Putri HMS Chandra Kusuma Iin Trilistiyanti Nugrahani, Iin Trilistiyanti Karyono Mintaroem Kusworini Handono Kusworini Handono Maimun Z Arthamin Maimun Zulhaidah Arthamin, Maimun Zulhaidah Maulana Wildan Seputra Melati Puspita Sari Niniek Burhan Nunuk S Muktiati Nunuk Sri Muktiati Nur Azizah Qatrunnada Naqiyyah Khusmitha S, Noorhamdani A Sardjono, Teguh Wahyu Shinta Oktya Wardhani Singgih Pujo Wahono Sri Winarsih Sumarno Reto Prawiro Sumarno Sumarno Tri Wahju Astuti Tri Wahju Astuti, Tri Wahju Triwahju Astuti Triwahju Astuti Umu Qonitun VALENTINA YURINA VALENTINA YURINA Wibi Riawan