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Biochemical study of CymJ, the putative regulator of cyclodextrin metabolism in Klebsiella oxytoca M5a1 Raras, Tri Y.M.; Boeck, August
Medical Journal of Indonesia Vol 16, No 2 (2007): April-June
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.338 KB) | DOI: 10.13181/mji.v16i2.258

Abstract

The products of ten genes clustered in two divergently oriented operons are required for the metabolism of cyclic oligoglucosides, the cyclodextrins, by Klebsiella oxytoca. The function of CymJ, the product of the promoter distal gene in one of the operons was studied. Over expression of cymJ in K. oxytoca led to strong reduction of the expression of the cym operons. This repression could be alleviated by addition of high concentration of α-cyclodextrin into the medium. There is a possible relationship between CymJ and CymD since the absence of CymD in a CymD deletion mutant prevented the repression effect of CymJ. An intriguing finding was that the presence of CymJ in large amount in the cell caused severe cell division inhibition leading to retardation of growth. This morphological change was paralleled by a significant increase in the susceptibility of K. oxytoca to ampicillin. (Med J Indones 2007; 16:69-77)Keywords: cym operon, putative regulator, over expression
Cloning and expression of pab gene of M. tuberculosis isolated from pulmonary TB patient in E.coli DH5α M. Raras, Tri Y.; Lyrawati, Diana
Medical Journal of Indonesia Vol 20, No 4 (2011): November
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4108.055 KB) | DOI: 10.13181/mji.v20i4.458

Abstract

Background: Mycobacterium tuberculosis antigen38 is a potent serodiagnostic agent containing two M. tuberculosisspecific B-cell epitopes. The high price of imported diagnostic agents hinders realization of fast clinical TB diagnosis in developing countries. Therefore, we produced recombinant antigen38 (recAg38M) from M. tuberculosis local strain, which might be used to produce economical tuberculosis serodiagnostic kit.Methods: Pab gene that was isolated from pulmonary TB patient in Malang was cloned into a plasmid vector (pGEMTeasy) to construct pMB38. The E.coli DH5α clone carrying pMb38 was selected on X-gal medium. The expression of pab was mediated using pPRoExHTc under the control of Trc promoter and E.coli DH5α as host.Results: Alignment of the pab sequence from the white E.coli DH5α clones with that of M. tuberculosis H37Rv showed 98% homology. The recombinant protein in which the signal peptide has been deleted to prevent the protein being secreted into medium was found in the cytoplasm.Conclusion: pab gene of M. tuberculosis isolated from a TB patient could be expressed in heterologous system in E.coliDH5α. (Med J Indones 2011; 20:247-54)Keywords: Mycobacterium tuberculosis, Pab gene expression, recombinant antigen38
Prolonged-heated High-Fat Diet Increase the Serum LDL Cholesterol Level and Induce the Early Atherosclerotic Plaque Development in Wistar Rats Yurina, Valentina; Yunita, Ema Pristi; Raras, Tri Yudani Mardining; Rudijanto, Achmad; Handono, Kusworini
Journal of Tropical Life Science Vol 9, No 1 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Rats are one of the most widely used animal models in health research. However, since rats are relatively resistant to atherosclerosis, the transgenic rats often used to study atherosclerosis in rats. Our study suggests a prolonged-heated lipid to induce atherosclerosis in rats, therefore provide a more low-cost option to study atherosclerosis in rats. Aims of this study is to compare the effect of heated high-fat diet (HFD) to the LDL and HDL cholesterol level and foam cell formation in the Wistar rat animal model. Rats were divided into three groups, control group was given with the normal diet, and the other two treated groups received oxidized HFD (heated HFD) and HFD, respectively. The heated HFD contain lard that was previously heated at 190°C for 24 h. Diet was given for 8 weeks. The serum LDL and HDL cholesterol level were measured before and 4th and 8th weeks after treatment with help of colorimetric measurement. The aorta analyzed for the foam cell formation after HE staining using the light microscope.  The prolonged- heated HFD significantly increased the LDL cholesterol from the 4th week of the treatment (p = 0.023). The rats aortic from HFD and heated HFD treated groups showed a mononuclear cells infiltration and the early foam cell formation. The heating process of the lipid caused lipid oxidation which significantly increased the LDL cholesterol level of the animal model and foam cells formation. This study suggests that Wistar rats with heated HFD could demonstrated early atherosclerosis plaque development.
Potensi Kadar Neopterin sebagai Biomarker untuk Monitoring Kemajuan Terapi TB Paru pada Anak Sholeh, Aunillah Hamid; Raras, TY Mardining; Kusuma, HMS Chandra
Jurnal Kedokteran Brawijaya Vol 27, No 4 (2013)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2013.027.04.7

Abstract

Kadar neopterin plasma ditemukan berhubungan dengan efikasi terapi pada tuberkulosis paru pasien dewasa. Penanda biologis untuk diagnosis tuberkulosis pada anak masih terus diteliti. Penelitian ini dilakukan untuk mengidentifikasi peran neopterin sebagai penanda biologis untuk mengevaluasi keberhasilan terapi standar tuberkulosis paru pada anak. Sebanyak 25 pasien dengan tuberkulosis paru diikuti selama enam bukan. Kadar neopterin plasma diukur dengan metode ELISA sebelum terapi, 2, 4, dan 6 bulan sesudah terapi. Sepuluh anak sehat ditetapkan sebagai kontrol. Hasil menunjukkan pada semua pasien kadar neopterin tertinggi didapatkan sebelum terapi dengan rerata 0,27 (SD 0,09c ) ng/mL. Kadar neopterin plasma pada kelompok kontrol  1,50 (SD 0,18) ng/mL, sebelum terapi 1,12 (SD 0,22) ng/mL, bulan kedua 1,08 (SD 0,22) ng/mL, bulan keempat 0,97 (SD 0,35) ng/mL, dan bulan keenam 0,45 (SD 0,29) ng/mL. Kadar neopterin tertinggi didapatkan sebelum terapi yang kemudian menurun secara bertahap sesuai terapi bersamaan dengan perbaikan gejala klinis. Kadar neopterin pada kontrol lebih tinggi dibandingkan pasien selama masa pengamatan. Dapat disimpulkan kadar neopterin plasma mempunyai potensi sebagai biomarker kemajuan terapi tuberkulosis paru anak.Kata Kunci: Anak, biomarker, neopterin plasma, terapi, tuberkulosis paru
PROFIL KADAR SOLUBLE UROKINASE PLASMINOGEN ACTIVATOR RECEPTOR (suPAR) PADA SERUM PENDERITA TUBERKULOSIS PARU (SEBAGAI MONITORING TERAPI) Astuti, Triwahju; MR, Tri Yudani; Riawan, Wibi; Muktiati, Nunuk Sri; Widjajanto, Edy
Jurnal Kedokteran Brawijaya Vol 24, No 1 (2008)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (136.873 KB) | DOI: 10.21776/ub.jkb.2008.024.01.4

Abstract

Dutch study shows that Upar expression content is significantly higher in tubercolusis patient’s psiphsy monosit compared to those in the healthy control group. So far, there is no biologic marker used whichcan accurately observe response improvement in the treatment of lung tubercolusis. The aim of this research is to investigate whether the serum level of soluble utokinase plasminogen activator receptor (suPAR) carries information in monitoring TB treatment for Lung Tuberculosis patients. suPAR was measured by ELISA in 21 individuals at the time of enrolment into observational cross sectional based on active tuberculosis  and 5 individuals as healthy control. There were 3 groups, 1). patients who had not started treatment (n=7),2). patients who had been treated for 2 months (n=7),  3). patients who had been treated for 6 months (n=7). Among groups, there were no difference in mean of body mass index, erythrocyte sedimentation rate and monocyte count. Patients positive for TB on direct  microscopy were 29% ( 6 from 21 patients) , 2 patients each groups. The suPAR levels were elevated in patients with active TB compared to healthy control (P<0,001). suPAR levels were highest in patients positive for TB on direct microscopy ( mean suPAR 4.455 ng/ml).
Efek Pemberian Protein Rekombinan Fusi ESAT6-CFP10 Mycobacterium tuberculosis terhadap Persentase IL2 dan IL10 yang Dipresentasikan Sel T CD8 pada Kultur PBMC Christianto, David; Raras, Tri Yudani Mardining; Sumarno, Sumarno; Arthamin, Maimun Zulhaidah; Astuti, Triwahju; Sardjono, Teguh Wahyu; S, Noorhamdani A
Jurnal Kedokteran Brawijaya Vol 30, No 3 (2019)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2019.030.03.7

Abstract

Keberhasilan vaksin BCG dalam memberikan perlindungan terhadap tuberkulosis (TB) pada orang dewasa di Indonesia belum optimal (37%) sehingga diperlukan vaksin alternatif yang lebih efektif. Protein rekombinan fusi ESAT6-CFP10 merupakan kandidat vaksin yang potensial. Penelitian dilakukan untuk menguji efektifitas protein rekombinan fusi ESAT6-CFP10 dalam meningkatkan ekspresi IL2 dan IL10 sel T CD8 yang memainkan peran penting dalam respon imun melawan TB. Pengujian kandidat vaksin dilakukan secara in vitro pada peripheral blood mononuclear cell (PBMC) dari kelompok sehat endemik TB, kelompok kontak TB, dan kelompok pasien TB dengan melihat persentase IL2 dan IL10 CD8. Setiap kelompok diberi perlakuan tanpa antigen, PPD, dan protein rekombinan fusi ESAT6-CFP10. Persentase IL2 meningkat secara signifikan dari kelompok sehat, kontak TB, hingga Pasien TB. Sebaliknya peningkatan persentase IL2 antar kelompok yang dipaparkan PPD tidak signifikan secara statistik (p=0,396). Persentase IL10 tidak menunjukkan perbedaan yang signifikan antar kelompoknya baik tanpa paparan antigen (p=0,617), PPD (p=0,351), maupun protein rekombinan fusi ESAT6-CFP10 (p=0,257). Didapatkan persentase IL2 yang tidak berbeda secara signifikan antar perlakuan pada kelompok sehat (p=0,309), kelompok kontak TB (p=0,318), dan kelompok pasien TB (p=0,424). Demikian juga dengan persentase IL10 yang tidak berbeda secara signifikan antar perlakuan pada kelompok sehat (p=0,908), kelompok kontak TB (p=0,352), dan kelompok pasien TB (p=0,776). Hal ini menunjukkan bahwa protein fusi rekombinan ESAT6-CFP10 dapat meningkatkan persentase IL2 tetapi tidak dengan IL10 meskipun secara statistik tidak signifikan.
Ekspresi IFN-γ dan IL-4 CD4+T Limfosit pada Tuberkulosis Kontak terhadap Antigen 38 Kda Mycobacterium tuberculosis Nugrahani, Iin Trilistiyanti; Kusuma, HMS Chandra; Raras, Tri Yudani Mardining; Arthamin, Maimun Zulhaidah; Astuti, Tri Wahju; Tanoerahardjo, Francisca
Jurnal Kedokteran Brawijaya Vol 28, No 4 (2015)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2015.028.04.8

Abstract

Mengendalikan epidemi tuberkulosis pada anak adalah prioritas kesehatan global. Efikasi vaksin Bacillus of Calmette and Guerin (BCG) sangat bervariasi sehingga memerlukan pengembangan vaksin baru. Antigen rekombinan 38 Kda Mycobacterium tuberculosis sebagai kandidat vaksin harus melalui uji imunogenitas. Tujuan studi ini untuk mengidentfikasi apakah antigen  38 Kda dapat menstimuli ekspresi IFN-γ dan IL-4 limfosit TCD4+ pada kultur PBMC anak dengan kontak TB. Studi ini dilakukan pada kultur PBMC dari 8 kontak TB dan 8 anak sehat yang diinduksi oleh antigen 38 Kda (kelompok 1), PPD (kelompok 2) dan tanpa perlakuan (kelompok 3). Ekspresi IFN-γ dan IL-4 limfosit TCD4+ diukur dengan flowcytometry. Rerata kadar IFN-γ untuk kontak TB tertinggi pada kelompok 3 (p=0,76), sedangkan rerata IL-4 tertinggi pada kelompok 2(p=0,68).RerataIFN-γ untukkelompok sehattertinggi pada kelompok3(p=0,78) sedangkanrerata IL-4 tertinggi pada kelompok 2 (p=0,32). Rerata ekspresi IFN-γ dan IL-4 yang diinduksi oleh antigen 38 Kda, masing-masing lebih tinggi pada kontakTB daripada subjek sehat (p=0,62 dan 0,39). Pengaruh respon imun yang protektif ditunjukkan oleh rasio ekspresi IFN-γ dan IL-4 yang lebih dari 1, baik pada kontak TB dan sehat (1,22 dan 1,28). Tidak ada perbedaan signifikan antara perlakuan pemberian antigen38 kDa, PPD dan tanpa perlakuan. Dapat disimpulkan bahwa antigen 38 Kda dapat menstimuli  ekspresi IFN-γ dan IL-4 limfosit TCD4+pada kultur PBMC kontak TB. Kata Kunci: Antigen rekombinan 38 Kda, Mycobacterium tuberculosis, IFN-γ, IL-4, limfositTCD4+
Ekstrak Etanol Teh Hijau (Camellia sinensis) Meningkatkan Kadar Folicle Stimulating Hormone (FSH) dan Jumlah Folikel Antral pada Tikus Betina yang Dipapar Sipermetrin Melati Puspita Sari; Sri Winarsih; Tri Yudani Mardining Raras; Karyono Mintaroem
Qanun Medika - Jurnal Kedokteran FK UMSurabaya Vol 2, No 2 (2018)
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jqm.v2i2.1559

Abstract

Latar Belakang: Sipermetrin adalah insektisida jenis piretroid sintetik yang dapat menganggu sistem reproduksi. Sipermetrin juga mampu menghasilkan  reactive oxygene species (ROS) sehingga bertanggung jawab menimbulkan terjadinya stres oksidatif. Teh hijau (Camellia sinensis) mengandung polifenol sehingga dapat menghambat radikal hidroksil dan enzim prooksidan. Tujuan: Penelitian ini bertujuan untuk membuktikan pemberian ekstrak etanol teh hijau dapat meningkatkan kadar FSH dan jumlah folikel antral pada tikus betina yang dipapar sipermetrin. Metode: Penelitian yaitu true experimental pada 25 ekor tikus betina galur wistar (Rattus norvegicus) yang dibagi menjadi 5 kelompok; KN: tanpa perlakuan, KP: sipermetrin 20 mg/kgBB/hari, P1: sipermetrin 20 mg/kgBB/hari + ekstrak etanol teh hijau 7 mg/kgBB/hari, P2: sipermetrin 20 mg/kgBB/hari + ekstrak etanol teh hijau 14 mg/kgBB/hari, P3: sipermetrin 20 mg/kgBB/hari + ekstrak etanol teh hijau 28 mg/kgBB/hari per oral selama 28 hari. Kadar FSH diukur dengan metode ELISA dan organ ovarium diambil untuk pemeriksaan jumlah folikel antral dengan metode pewarnaan hematoxylin eosin. Hasil: Kadar FSH dan jumlah folikel antral pada tikus betina menunjukkan hubungan yang positif dan kuat dengan nilai koefisien korelasi 0,742 yang berarti peningkatan kadar FSH diikuti penambahan jumlah folikel antral. Ada perbedaan bermakna pada kadar FSH (p=0,005) dan jumlah folikel antral (p=0,001) antara kelompok kontrol dengan kelompok perlakuan. Kesimpulan: Pemberian ekstrak etanol teh hijau dapat meningkatkan kadar FSH dan jumlah folikel antral pada tikus betina yang dipapar sipermetrin.
Probiotics Lactobacillus reuteri increase levels of β-Defensin1, sIgA and decrease number of Staphylococcus aureus bacteria colonies in vaginal mucosa on puerperal mice model infected with Staphylococcus aureus Nur Azizah; Umu Qonitun; Tri Yudani Mardining Raras; Sumarno Reto Prawiro
Jurnal Kebidanan Vol 9, No 1 (2020): JURNAL KEBIDANAN
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26714/jk.9.1.2020.71-80

Abstract

Puerperal Infection is a bacterial infection in the genitalia and female reproduction system, which cause the highest mortality among women who post give birth in recent years. The causative agent of this infection is Staphylococcus aureus (S. aureus). Alternative therapy using probiotics such as Lactobacillus reuteri was developed to reduce the increasing incidence of antibiotic resistance. This article studies the effect of probiotics L. reuteri increasing β-defensin1 levels, sIgA levels, and decreasing the number of bacteria S. aureus colonies in the vaginal mucosa on puerperal mice model that induced with S. aureus bacteria. Mice BALB/c were used as an animal model and divided into four different groups. The measurement β-defensin1 levels, sIgA levels, and the number of bacteria S. aureus colonies were conducted in one and three days postpartum. Enzyme-Linked Immunosorbent Assay (ELISA) method was to measure performed for β-defensin1 levels, and sIgA levels, Total plate count was used for the quantity of bacteria S. aureus colonies. β-defensin1 and sIgA levels showed a similar pattern and elevated significantly in all treated group compared to control. The highest value was obtained from a group that administered with L. reuteri and induced with S. aureus in all measurements. The levels of β-defensin1 and sIgA in three days postpartum were higher than that day one. The number of S. aureus colonies was lower in the treated group compare that of the positive control. The average number of bacteria in one day postpartum was higher than three days. The administration of L. reuteri and or induction of S. aureus could increase the level of β-defensin1 and sIgA but reduced decrease in the number of bacteria S. aureus colonies/
Correlation between soluble urokinase plasminogen activator receptor with CD4 T lymphocyte and WHO clinical staging of HIV infection Shinta Oktya Wardhani; Niniek Burhan; Gatoet Ismanoe; Tri Yudani
Indonesian Journal of Tropical and Infectious Disease Vol. 1 No. 1 (2010)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (476.276 KB) | DOI: 10.20473/ijtid.v1i1.3720

Abstract

The urokinase-type plasminogen activator (uPA) and its receptor play a key role in pericellular proteolysis, cell migration and signal transduction. Previous study showed that suPAR could be used as an independent prognostic marker of disease progression in HIV-1 patients.1,17 Immune status of HIV patient and progressivity of disease are important parameters used as clinical concideration before initiating anti retroviral treatment and for monitoring treatment effectivity. Recently immune status of HIV patients is determined by CD4 T lymphocyte counting which represents the remaining healthy lymphocyte T expressing CD4 that very expensive and need special laboratory equipment. Destruction and shedding of T lymphocyte, macrophage and natural killer cell will deliver soluble urokinase plasminogen activator receptor, a surface protein which is expressed by those cells and can be measured by ELISA8,9,11. This study objective is to determine correlation between suPAR plasma concentration and CD4 T lymphocyte and WHO clinical stagging of HIV infection. Study subjects. Fifty four naieve HIV-1-infected patients (32 males, and 22 females) are participant in a cross sectional study enrolled on 22 November 2007 until 31 july 2008 at the department of infectious disease Saiful Anwar Hospital, Malang, Indonesia. Blood sampling. Two blood samples were drawn before treatment, CD4 counts were measured with an Epics XL-MCL Coulter flowcytometer. EDTA plasma for suPAR measurement was stored at -80°C. Data are presented as mean±standart deviation. P<0.05 is considered significant. Statistical calculations were done using SSPS 15. Patients (n = 54) enrolled and clustered according to WHO clinical stage ( I - IV) at inclusion. All HIV-infected patients had measurable levels of plasma suPAR with a median value of 8,9 ng/mL(range 1,65-29,7 ng/mL). Pearson correlation demonstrated a weak but significant negative between suPAR and CD4 T lymphocyte count (p=-0.634, p<.0005). suPAR level positively correlated with the WHO-defined clinical stages (P< .0005, spearman correlation test, r=0,87). There were significant difference between each stage i.e I(1,6± o,61ng/mL), II(3.04±1.03 ng/mL), III (10.53±7.1ng/mL) and IV (20.42±10.81ng/mL) (P< .0005, Spearman test). In addition pearson correlation demonstrated a weak but significant negative correlation between suPAR and CD4 count (p=-0.66; P<.0005). There were negative significant correlatio between CD4 count and suPAR level, suggested that suPAR could provide as a complementary biological marker for HIV-1 although it can not replace the CD4 count. SuPAR plasma concentration and clinical stage give significantly correlation with WHO clinical staging of HIV infection.