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Genetic Variability of Galoba Durian (Amomum spp) Center Mollucas and North Halmahera Based on RAPD Katerina Jagagura Peilouw; Adriana Hiariej; Anneke Pesik
Jurnal Penelitian Pendidikan IPA Vol. 8 No. 2 (2022): April
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v8i2.1353

Abstract

Galoba Durian is one of the endemic flora of Maluku, which is used as a medicinal plant and essential oil with red and green fruit colors. This study aimed to analyze the genetic variation of Galoba Durian in Central Maluku and North Halmahera based on the molecular marker of RAPD. This study began with a survey of sampling, isolation, and amplification of DNA using five RAPD primers. The results showed that all DNA bands were polymorphic as many as 67 with genetic similarity ranging from 20% - 31%, which grouped four samples into two groups. The first group is red Galoba Durian from North Halmahera (S1), red (S3) and green (S4) from Central Maluku, while the second group is green Galoba Durian from North Halmahera (S2).
UJI AKTIVITAS SEDIAAN SPRAY HAND SANITIZER KOMBINASI EKSTRAK DAUN SIRIH HIJAU DAN DAUN SERAI SEBAGAI ANTIBAKTERI TERHADAP Staphylococcus aureus Mutia M Tuasalamony; Cecilia Anna Seumahu; Anneke Pesik
Jurnal Biosilampari : Jurnal Biologi Vol 4 No 2 (2022): Biosilampari
Publisher : LP4MK STKIP PGRI Lubuklinggau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31540/biosilampari.v4i2.1515

Abstract

The content of extracts of green betel leaf and lemongrass leaves have antibacterial compounds to inhibit the growth of Staphylococcus aureus. The purpose of this study was to determine the antibacterial activity of the spray hand sanitizer preparation of a combination of green betel leaf extract and lemongrass against Staphylococcus aureus. This type of research is an experimental laboratory. Green betel leaves and lemongrass leaves were partially macerated, formulated to be hand sanitizers in a ratio of 1:2, 2:1, and 2:2. The formula was tested for antibacterial activity by the dilution method. The results showed that the hand sanitizer combination of green betel leaf extract and lemongrass had antibacterial activity against Staphylococcus aureus with a bactericidal formula of 0 CFU/ml and total bacteria with an antiseptic formula of 0 CFU. From this research, it is known that the spray hand sanitizer combination of green betel leaf extract and lemongrass is effective in inhibiting and killing Staphylococcus aureus.
Analisis Beta Karoten dan Vitamin pada Kulit dan Daging Buah Pisang Tongka Langit (Musa Troglodytarum L.) di Kota AmbonAROTEN DAN VITAMIN PADA KULIT DAN DAGING BUAH PISANG TONGKA LANGIT (Musa troglodytarum L.) DI KOTA AMBON Owend Paul Letelay; Adriana Hiariej; Anneke Pesik
Jurnal Agritechno Jurnal Agritechno, Vol. 13, Number 1, April 2020
Publisher : Depertemen Teknologi Pertanian Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (363.672 KB) | DOI: 10.20956/at.v13i1.243

Abstract

Banana Tongka Langit with fruit bunches soaring up has varied morphological characters including fruit size (long and short), fruit flesh color (yellow to orange) and different banana peel colors (yellow, yellowish orange, reddish orange, reddish brown, and brown) brownish orange) which indicates the presence of beta carotene content. The purpose of the study was to analyze the content of beta carotene and vitamins in the skin and flesh of the Tongka Langit banana using the High Performance Liquid Chromatography method. The results of the analysis of the highest beta carotene content in long fruit flesh is 65.33 mg / 100 and the lowest in short fruit meat is 22.31 mg / 100g. The most vitamin A found in short fruit skin 2260.63 mg / 100g and long fruit skin has the lowest value. The highest value of Vitamin B content is found in long fruit peels at 0.11 mg / 100g and the lowest in long fruit flesh and short fruit peels at 0.09 mg / 100g. The most vitamin C found in long fruit flesh is 28.48 mg / 100g, and the lowest in long fruit skin is 11.99 mg / 100g. The highest vitamin E was found in long fruit peels at 0.41 mg / 100g and the lowest was in short fruit flesh at 0.21 mg / 100g, while vitamin D was not found in all samples.
KESADARAN LITERASI DIGITAL: PELUANG, TANTANGAN DAN KERENTANANNYA BAGI KOMUNITAS LAUT-PULAU (Suatu Pemikiran) Pieter Agusthinus Riupassa; Anneke Pesik
Pattimura Proceeding 2022: PROSIDING SEMINAR NASIONAL DPD HIMPUNAN ALUMNI IPB MALUKU
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Digital literacy has been supported by the development of information technology in Industry 4.0 which has significantly changed and enabled many opportunities for advancement in the community learning dimension. The system connected to the internet network and the availability of information (i.e. science, technology, and art) is a golden opportunity to create the achievements of the marine-isle community, which is always under the stigma of being fallen behind, so that they can get out of the poverty trap into a space of empowerment and mental readiness to enter the era of a new revolution. The approach were used socio-scientific interpretive and narrative method. This article aim is to reflect and capture the existence of the marine-isle community to inspire and motivate young learners to take advantage of easy access to information, and pinned a number of mentality attitudes (soft skills) to achieve success in life. A number of high awareness attitudes in changing the way of thinking and behavior patterns to motivate persons and our society. Thus, at least at the social level, inherent values, hidden internally to develop into young learners with digital characters who are adaptive, proactive, and dynamic. Otherwise, with anxiety, the community will be crushed and shackled by the hustle and bustle of digital advances
Epidermal Structure and Leaf Stomata of Several Accessions of Banana Plants (Musa spp.) Gebiana Nyainleta; Anneke Pesik; Adriana Hiariej
Jurnal Penelitian Pendidikan IPA Vol. 8 No. 6 (2022): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v8i6.2194

Abstract

This study aimed to determine the structure of epidermal cells and leaf stomata in Jarum, Merah, Tanduk, Kepok, and Pei or wild banana. The type of research used was quantitative to calculate the number of epidermis, epidermal cell size, number of stomata, and stomata index. Meanwhile, qualitatively described the structure of epidermal cells, epidermal cell shape, stomata shape, and stomata type of banana leaves based on the longitudinal incision method. The results showed that four accessions of bananas have epidermal cell shapes: rectangular, pentagon, hexagon, heptagon, octagonal, and regularly length. Meanwhile, Merah have different epidermal cell shapes, namely rectangular, pentagon, hexagon, and irregular, with untidy arrangements. The stomata of the five samples are surrounded by four to six subsidiary cells and are kidney-shaped. Based on the location of the leaf surface, stomata in five banana accessions have an amphistomatic type. Pei or wild bananas have a long epidermal size compared to the other four species but have a small number of epidermis. Then, the number of stomata in all banana species differs on the upper and lower surfaces. The number of stomata is more on the lower surface of the leaves. Stomata index in all banana species is low
ANTIBACTERIAL ACTIVITY AND INHIBITION OF BIOFILM OF Hibiscus tiliaceus STEM BARK METHANOL EXTRACT TO Streptococcus mutans GROWTH Wati Lukaraja; Widya Lessy; Cecilia Anna Seumahu; Anneke Pesik
RUMPHIUS Vol 2 No 2 (2020): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv2i2p066-071

Abstract

Dental caries is an infectious disease that occurs in the oral cavity preceded by the formation of dental plaque or biofilm. This study aims to examine the inhibition and biofilm inhibition of hibiscus tree bark extract (Hibiscus tiliaceus). The test methods used were Minimum Inhibitory Concentration (MIC) and Minimum Killing Concentration (MBC) while the analysis of biofilm inhibition used UV-VIS spectrophotometry at a wavelength of 580 nm with McFarlandII standard (6x108 CFU/ml). The results showed that the methanol extract of hibiscus bark at a small concentration of 0.5% could inhibit and kill Streptococcus mutans as indicated by the number of colonies that grew less with a value of 3.34 x 107 CFU/ml in colonies that grew at an extract concentration of 2. % and 1%. Biofilm inhibition is shown by the decreasing absorbance value due to the increased extract concentration so that less biofilm formation occurs.