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Correlation between Vertebral Length and Body Height (Os. Vertebralis) in Indonesian People Yudianto, Ahmad; Wibowo, Ariyanto
Majalah Kedokteran Bandung Vol 51, No 1 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (556.325 KB) | DOI: 10.15395/mkb.v51n1.1472

Abstract

Identification of unidentified living and dead human bodies can be carried out for the purposes of criminal investigation and other police duties. Among the most important information to identify one’s identity is the one obtained from the results of measuring the victim’s height. Thus, it is necessary to determine the height of a body of unidentified identity during the examination. To date, the correlation between the body height and vertebrae has not been widely known. The purpose of this present study was to determine the correlation between the body height and the length of the vertebrae. The study was conducted in Dr. Soetomo General Hospital from January to July 2018. This was an observational analytical study on 57 samples consisting of 32 male and 25 female samples. Results indicated that the correlation between the vertebral length (thoracic vertebra (X1) and lumbar vertebra (X2)) and the body height as determined using the following equations: Y=47.428+2.991 X1+2.13 X2 cm (male); Y=-32.496+3.800 X1+5.549 X2cm (female); show that there are quite strong when analyzed using t-test (r=0.799 for male and r=0.908 for female). In conclusions, this regression formula can be used in estimating the height found only in bones without other long bones.  Korelasi Antara Panjang Tulang Belakang (OS.Vertebralis) dengan Tinggi Badan pada Orang Indonesia  Identifikasi tubuh manusia yang tidak dikenal, baik yang masih hidup maupun yang sudah meninggal dapat dilakukan bagi kepentingan penyidikan perkara pidana dan bagi tugas kepolisian yang lain. Salah satu informasi yang sangat penting dapat digunakan untuk melacak identitas seseorang adalah informasi yang didapatkan dari hasil pengukuran tinggi badan korban. Oleh karena itu, pada waktu dilakukan pemeriksaan jenasah yang tidak diketahui identitasnya, perlu ditentukan tinggi badannya. Sejauh ini korelasi tinggi badan dengan panjang ruas tulang belakang belum banyak diketahui. Penelitian bertujuan mengetahui korelasi tinggi badan dengan panjang ruas tulang belakang. Penelitian dilakukan di RSUD Dr. Soetomo periode Januari sampai Juli 2018. Metode penelitian yang digunakan observasional analitik yang dilakukan pada 57 sampel dengan rincian 32 sampel pria dan 25 sampel wanita. Hasil penelitian, korelasi panjang tulang belakang (vertebra torakalis (X1) dan vertebra lumbalis (X2) dengan tinggi badan dengan uji t didapatkan persamaan regresi: Y=47,428+2,991 X1+2,13 X2 cm (laki-laki); Y=-32,496+3,800 X1+5,549 X2 cm (wanita). Nilai korelasi yang cukup kuat, r untuk laki-laki: 0,799 sedangkan untuk wanita 0,908. Simpulan, Formula regresi ini dapat digunakan untuk memperkirakan ketinggian yang ditemukan hanya pada tulang tanpa tulang panjang lainnya.
Genetic variation analysis and kinship relationship between Dayak Ngaju tribe and Dayak Bukit tribe through examination of core DNA of Bukit CODIS STR Locus (combine DNA index system) 13 for the purpose of Forensic Identification Iwan Aflanie1 , Wening Prastowo2 , Roselina Panghiyangani3 , Ahmad Yudianto4 , Toetik Koesbardiati5
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 3 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i3.10519

Abstract

Dayak Ngaju tribe lived scattered along the Kapuas and Kahayan river. Meanwhile, the Dayak Bukit tribe lived scattered the mountain and valley area. Kinship between Dayak Bukit and Dayak Ngaju tribe is still under debate. DNA examination is one of the most reliable methods in determining personal identity, community, and kinship relationship. FBI has recommended DNA analysis using 13 short tandem repeat loci, known as CODIS 13 for DNA test. We used the system to identify the difference between Dayak Ngaju and Dayak Bukit tribe and analyze the kinship between these two tribes. There are loci and alleles that always owned by each individual of Dayak Ngaju tribe. The Dayak Ngaju characteristic which cannot be discovered in Dayak Bukit tribe are TPOX allele 6 locus, FGA allele 25.2 locus, CSF allele 8, 10.3, 11 locus, VWA allele 13, 15.1 locus, D18S51 allele 9, 13, 18, 20 locus, D21S11 allele 34 locus, D7S870 allele 12.1 locus. Dayak Bukit tribe has locus and allele that owned by each individual of Dayak Bukit tribe. The Dayak Bukit tribe characteristic which cannot be discovered in Dayak Ngaju tribe are FGA alleles 18.3, 19.3, 21.2, 23, 23.1 locus, CSF allele 28.2 locus, D7S870 allele 7.3 and allele 8 locus. The similarity of Dayak Ngaju tribe and Dayak Bukit tribe lied on 5 dominant loci named THO1 allele 9.3 locus, D5S818 allele 11, VWA allele 16, D3S1358 allele 16, D13S317 allele 8, but these loci are also found in Banjar Hulu tribe. The similarity that only discovered in Dayak Ngaju and Dayak Bukit tribe are located in TPOX allele 7 locus, FGA allele 20.2, 20.3, and D3S1358 allele 15.2. According to the research results above, it can conclude that Dayak Ngaju tribe doesn’t have a close kinship to the Dayak Bukit tribe.
The Use of Touch DNA Analysis in Forensic Identification Focusing on STR CODIS LOCI THO1, CSF1PO and TPOX Ahmad Yudianto1 , Simon Martin Manyanza Nzilibili2 , Pudji Harjanto2 , Fery Setiawan2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 3 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i3.10667

Abstract

Introduction:Identification through DNA analysis is an accurate diagnostic tool. DNA analysis is via Variable Number of Tandem Repeat (VNTR) and Restriction Fragment Length Polymorphisms (RFLP). Up to date, blood spots/blood, sperm spots, vaginal swabs, buccal swabs and bones are specimens that are widely used in the field of forensics for DNA analysis. At the crime scene, the perpetrator’s skin may accidentally be in contact with surrounding objects thereby transferring trace evidence to the objects. Method and Materials: Laboratory observation to demonstrate identification through DNA isolation from the objects that are touched (touch DNA), using the STR CODIS locus, with a momentary research design. DNA was isolated from buccal swabs and swab properties (watches and mobile phones) from volunteers who have signed the consent form. A total of ten samples were used in this study. Results and Discussion: Mean levels of DNA [UV-Visible Spectrophotometer] buccal swab: 167.89 ± 85.71 µg / ml, watch swab: 59.19 ± 5.58 µg / ml, mobile swab: 38.09 ± 2.12 µg / ml and the purity of the buccal swab DNA: 1.79 ± 0.71, the watch swab: 1.69 ± 0.76, the mobile swab:1,53 ± 0,56. Visualization of PCR products onPolyacrylamide Agarose Composite Gel Electrophoresis [PAGE] stained with Silver and amplified using the standard primers THOI, TPOX and CSF1POfor STR CODIS showed a 100% detection of amplicons. Allele profiles on all samples of STR CODIS were a match or identical to the positive control K562. Conclusion:Property (handphone and watch) swabs can be used as alternative materials in forensic identification using Touch DNA analysis. It was able to be isolated and amplified by using Polymerase Chain Reaction on the STR CODIS loci THO1, CSF1PO and TPOX
Capsaicin’s Inhibition Effects on Biofilm Aerococcus Viridans Fery Setiawan1 , Jenny Sunariani2 , Ahmad Yudianto3 , Latief Mooduto4 , Eta Radhianto5
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 3 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i3.10698

Abstract

Introduction: Aerococci has colony shape with Streptococcus viridans, so that it is usually missdiagnosed between of them. It has high difficulty on its identification, because it is rarely to be found and resistant of Antibiotic grup, called penicilin and vancomycin. Material and Method: This research used two kinds of samples, such as: fried food sold on the road and toothbrush obtained from ten respondens. Then, it needs inducing its biofilm and measuring by Tissue Culture Plate (TCP) methode and ELISA. Result: ANOVA one-way revealed that extract capsaicin has anti biofilm effect on 12.5% concentration with p=0,0000 (p<0,05) among each group. Conclusion: Extract capsaicin can be used as an alternative herbal agent to treat infection caused by Aerococcus viridans because it has antibiofilm effect. As known before, biofilm causes antibiotic resistance to treat Aerococcus viridans’s infection.
Identification of DNA Corpse in Ordo Diptera Familia Calliphoridae Larvae in CSF1PO, TH01, and TPOX Locus Using Polymerase Chain Reaction (PCR) Methods Tutik Purwanti1 , Ahmad Yudianto1 , Mochammad Soekry Erfan Kusuma1
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.12175

Abstract

Introduction: Biomolecular techniques using DNA are known to have high accuracy in the identification ofdecaying bodies. In the process of identification, larvae found in corpses that have undergone decay can beused as alternative biological evidence to estimate the time of death.Aim: To identify the transfer of body DNA to the larvae of the fly ordo Diptera family Calliphoridae and theprocess of DNA transfer of decomposed bodies.Method: blood from the postmortem corpse was taken as a control, then soft tissue was taken as adecomposition product for the breeding of Dipdo family Calliphoridae larvae. The protocol used is DNAprofiling using the Short Tandem Repeat (STR) locus such as CSF1PO, TH01, and TPOX. The results ofDNA extraction from the postmortem body and DNA extraction results obtained from the skin of the larvaeand the digestive system of the larvae are then matched.Result: The results of this study found that there was DNA transfer in larvae proved by the matching ofDNA at the STR loci on the skin and the digestive system of larvae with the STR locus in blood taken fromthe postmortem bodyConclusion: the presence of decomposed corpse DNA decomposition to the larvae of members of the ordercalled Calliphorideae family which can be detected through the results of nuclear DNA visualization at theCSF1PO, TPOX, and TH01 loci using the PCR method
The effect of temperature and storage time of cuccal swabs on FGA and D13S317 loci with the STR PCR method Masjkur, Indah Nuraini; Arfianti, Evy; Yudianto, Ahmad; Furqoni, Abdul Hadi; A’yun, Qurrota
Jurnal Teknologi Laboratorium Vol 9 No 2 (2020): 2020 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29238/teknolabjournal.v9i2.243

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The samples used for forensic DNA analysis in living individuals are usually blood and buccal swabs, however, blood collection requires an invasive method that can cause discomfort, thus a buccal swab can be a good choice for individuals examined, especially children. This study aimed to determine the effect of temperature and storage time of buccal swabs on the quantity of DNA as material for DNA examination in the forensic field. This study was a laboratory experiment to determine the effect after treatment. Buccal swab samples were 48 and divided into 2 temperature groups, namely room temperature (RT) and 4℃. The division of the temperature groups was also observed with time differences, namely 1, 3, 5, 7 days. EDNA extraction used the DNAzol method and DNA quantification used a Spectrophotometer. The PCR process was carried out with STR primers FGA and D13S317 loci. The visualization stage used acrylamide gel and silver staining. The results of this study prove that there is an effect of temperature and storage time of buccal swab samples. The longer the treatment time, the lower the DNA level. With statistical analysis, it is obtained p-value of <0.005, it can be concluded that there are significant differences in DNA levels at the temperature and storage time treatments of the buccal swab sample. The results of DNA visualization at the FGA and D13S317 loci using the STR PCR method in this study can still be detected and can be used as a reference for examination in forensic cases.
Analysis of Human Decomposition Effect on DNA Quality with Short Tandem Repeats [STRS] Combined Index DNA System [CODIS] Nily Sulistyorini, Ahmad Yudianto1 , Ma’rifatul Ula2 , Renny Sumino3 , Fery Setiawan4
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.12126

Abstract

Introduction: Personal identification is a problem in criminal or civil cases. Exact determination of personalidentity is very important in investigation as any error can be fatal in the judicial process. The process ofidentification that is often used is through DNA analysis. The problem that often becomes a serious problemfor both forensic DNA expert and other DNA expert is the condition of degraded DNA.Method and Materials: This study was conducted by analysing DNA damage patterns using CODIS STR(Short Tandem Repeat) markers to effect the decay process. The type of research used by experimentallaboratories, with research design used is time series.Results and Discussion: The result of DNA sample extraction from Psoas and Masseter muscle samplesshows the average minimum range of DNA levels for DNA typing is 0.25 ng with purity of 1.8 – 2(1). Theresult of this study prove the effect of decomposition on DNA concentration on each muscle. This study isalso showed a decrease in levels and purity in the samples of Psoas and Masseter muscle tissue buried in soil,sea/salt water and river/fresh water from day 1, 7, 14, 20 and 40.Conclusion: Psoas and Masseter muscle can be alternative material for forensic identification. The successof sequence mapping pattern from STR loci are TPOX, THO1, and CS1PO according to each GC content.GC content of THO1 and TPOX has the same relative value of 0,48, compared to CSF1PO value of 0,33.
Karakteristik Genetik Populasi Kuno Pulau Bali: Sanur dan Gilimanuk Rusyad Adi Suryanto; Toetik Koesbardiati; Delita Bayu Murti; Ahmad Yudianto; Anak Agung Putu Santiasa Putra
Berkala Arkeologi Sangkhakala Vol 17 No 1 (2014)
Publisher : Balai Arkeologi Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1345.944 KB) | DOI: 10.24832/bas.v17i1.92

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AbstractThe study of ancient human migration and peopling in Indonesia still raises debate until now, both from the perspective of biological anthropology, human genetics or archaeological. The debate was always open space again to do some research about that. We concentrated with samples of ancient Bali, the findings of human remains from Gilimanuk (Melaya, Jembrana) and Semawang (Sanur, Denpasar). Relatively, Bali is an island located in the centre of Indonesian Archipelago, which may represent a major pathway of human migration and distribution according to the outer arc islands. The research aimed to describe human genetic variation of the two archeological sites of ancient Bali. Based locus short tandem repeats (STR) combined DNA index system (CODIS), which CSF1PO, TH01 and TP0X, the research took a sample of six individual human ancient Bali, which includes each of the three individual from Semawang and Gilimanuk site. The process of genetic research has been done at the Institute of Tropical Disease Laboratory of Human Genetics, Airlangga University. Semawang and Gilimanuk derived from different populations based on the analysis of its CTT loci visualization. The results with reference to all possible aspects of archaeology and biological anthropology further enrich the wealth of knowledge about human migration events in Indonesia around the Neolithic period, the early times of increasingly massive mongoloid migrations to the Archipelago region. The results also further strengthen the results of previous genetic studies of Bali population. Balinese has undergone a genetic mixture of various immigrant populations since the Neolithic period.AbstrakPenelitian migrasi dan penghunian manusia kuno di Indonesia masih memunculkan perdebatan sampai kini, baik dari perspektif antropologi biologis, genetika manusia atau arkeologis. Perdebatan itu selalu membuka ruang lagi untuk melakukan penelitian perihal itu. Kali ini kami berkonsentrasi dengan sampel Bali Kuno, yakni temuan sisa-sisa manusia dari Gilimanuk (Melaya, Jembrana) dan Semawang (Sanur, Denpasar). Bali merupakan pulau yang relatif terletak di tengah gugusan kepulauan Indonesia, di mana dapat mewakili jalur besar migrasi dan persebaran manusia seturut rute pulau-pulau busur luarnya. Penelitian ini bertujuan untuk mendeskripsikan variasi genetik manusia kuno dari dua situs arkeologis Bali itu. Berdasarkan lokus short tandem repeats (STR) combined DNA index system (CODIS), yakni CSF1PO, TH01 dan TP0X, penelitian ini mengambil sampel enam individu manusia Bali Kuno, yang meliputi masing-masing tiga individu Semawang dan Gilimanuk. Proses penelitian genetik itu telah dikerjakan di Laboratory of Human Genetics, Institute of Tropical Disease, Universitas Airlangga. Sampel Semawang dan Gilimanuk berasal dari populasi yang berbeda berdasarkan analisis visualisasi lokus CTT-nya. Hasil penelitian ini dengan merujuk semua kemungkinan aspek arkeologis dan antropologi biologisnya makin memperkaya khazanah pengetahuan tentang peristiwa migrasi manusia di Indonesia sekitar masa Neolitik, yang menjadi masa awal makin masifnya migrasi Mongoloid ke kawasan Nusantara. Hasil penelitian ini juga makin menguatkan hasil-hasil penelitian genetika populasi Bali sebelumnya bahwa populasi Bali dari sejak Neolitik sampai sekitar masa yang lebih resen diturunkan oleh banyak leluhur atau banyak sumber gen. Penduduk Bali telah mengalami percampuran genetik dari berbagai populasi pendatang sejak Neolitik atau awal Tarikh Masehi.
VARIATION DIFFERENCES OF PHALANX DISTAL FINGER PRINT PATTERN ON KLEPTOMANIA PATIENS AND NON-PATIENTS IN POLICE RESORT JEMBER Amalia Rozaiza Ightikhoma; Nazaratun Thaiyibah; Ahmad Yudianto
Jurnal Ilmu Kesehatan Vol 8 No 1 (2020): Jurnal Ilmu Kesehatan
Publisher : Universitas Muhammadiyah Kalimantan Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30650/jik.v8i1.1354

Abstract

Phalanx distal pattern is a curve appeared and formed an unchangeable pattern unless it is caused by a severe trauma. Phalanx distal patterns are classified into 9, namely, Plain Arch, Tentarch, Ulnar loop, and Accidental. In order to find out suspect’s identity, Indonesia Autometic Print Identification System (INAFIS) used 3 stages, which one of them is to determine pattern variation. Phalanx distal fingerprint on human can be utilized as to identify a person, whereas no one has resembled phalanx distal fingerprint even on twins. In this research, the researcher analyzed tendentious pattern variation of phalanx distal pattern on kleptomaniac in police resort office Jember. Generally, phalanx distal fingerprint pattern found on kleptomaniac tendentiously has unique pattern plain arch as the third most apparent pattern beside unlar loop and whorl, meanwhile it was found radial loop on non-patient as the third most apparent beside unlar loop and whorl which generally was phalanx distal fingerprint.
Molecular Review Covid19 from the Pathogenesis and Transmission Aspect Fery Setiawan; Heni Puspitasari; Jenny Sunariani; Ahmad Yudianto
JURNAL KESEHATAN LINGKUNGAN Vol. 12 No. 1si (2020): SPECIAL ISSUE
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jkl.v12i1si.2020.93-103

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Introduction: Corona disease-19 virus (COVID-19) spread and caused a pandemic that affected people all over the world. COVID-19 is also called Severe Acute Respiratory Syndrome-Coronavirus Disease (SARS-CoV). Discussion: COVID-19 is a β-coronavirus serotype which is a single strain of RNA virus and was an outbreak in 2002 (SARS-CoV) and 2012 (MERS-CoV). COVID-19 has Open Reading Frames (ORFs) consisting of Spikes (S), Envelopes (E), Membranes (M), and Nucleocapsids (N) with S parts being a glycoprotein that can attach to receptors owned by host cells, the receptors are CD 26, ACE-2, Ezrin, and Cyclophilins with the main receptor being ACE-2 in the lung organs. Process would evoke a host body's immune response consisting of natural and adaptive immune systems, involving the Antigen Presenting Cell (APC) system which consists of two, namely: Major Histocompatibility Complex (MHC) class I and II. APC could also generate adaptive immune system, consists of B and T cells. COVID-19 had the ability to survive in B and T cells, so that cytokine-chemokine secretion continues to be known as cytokine storm that trigger Acute Respiratory Distress Syndrome (ARDS) and death. Conclusion: The recovery prognosis of COVID-19 depended on the detection of COVID-19 patients because it was related to the severity of ARDS, so the earlier it was detected, the greater the chance of recovery.