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All Journal International Journal of Evaluation and Research in Education (IJERE) HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Pendidikan Humaniora Jurnal Pendidikan Sains El-Hayah : Jurnal Biologi Journal of Education and Learning (EduLearn) Biotropika KEMBARA Bioeksperimen: Jurnal Penelitian Biologi Indonesian Language Education and Literature Jurnal Pendidikan: Teori, Penelitian, dan Pengembangan THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Jurnal Pendidikan Ekonomi & Bisnis (Edisi Elektronik) Biosfer: Jurnal Pendidikan Biologi Diglosia: Jurnal Kajian Bahasa, Sastra, dan Pengajarannya Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Satwika : Kajian Ilmu Budaya dan Perubahan Sosial Hasta Wiyata Jurnal Onoma: Pendidikan, Bahasa, dan Sastra Bio-Inoved : Jurnal Biologi-Inovasi Pendidikan Ghancaran: Jurnal Pendidikan Bahasa dan Sastra Indonesia Jurnal Riset Biologi dan Aplikasinya Wahana Dedikasi : Jurnal PkM Ilmu Kependidikan Proceeding Biology Education Conference Berkala Penelitian Hayati Borneo Journal of Biology Education (BJBE) Bahasa dan Seni: Jurnal Bahasa, Sastra, Seni, dan Pengajarannya Journal of Literature and Education Prosiding Seminar Nasional Pengabdian kepada Masyarakat (SINAPMAS)
Dwi Listyorini
Universitas Negeri Malang
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Other

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PROSES REGENERASI SIRIP EKOR PADA IKAN ZEBRA Sari, Nila Kartika; Listyorini, Dwi; Gofur, Abdul
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol 1 No 01 (2016): Edubiotik
Publisher : Program Studi Pendidikan Biologi, IKIP Budi Utomo Malang

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Abstract

The caudal fin of Zebra fish as a model for studies regeneration both in terms of process and study of molecular analysis. This research is a descriptive exploratory. A sample of zebra fish (Danio rerio) 4-6 months of age as much as 30 individuals. The regeneration caudal fin of zebrafish observed by microscope with cutting vertically. The parameters observed were: 1) the time required for the regeneration process, 2) The regeneration process in details covering and, 3) Type of regeneration. Data in the form of the regeneration process ranging from wound healing to the original length obtained are collected in the form of digital photos. The results showed that the time required to regenerate the caudal fin to recover to normal at a temperature of 22-31° C was 22.25 ± 1.63 days for a model of vertical cuts. The regeneration process begins with the events of wound healing (H1) followed by the formation of blastema and will radius (H2) and continued differentiation characterized by the process of angiogenesis (H2). Regenerative development begins with the formation of the fingers and the growth of the peripheral areas tail fins faster than the medial region (H3-H8). End process regeneration in caudal fin of zebrafish are included in the development is the formation of regenerative segment radius starting from the proximal to the distal portion regenerat regenerat (H9 - ± H21). From the results of this research note that caudal fin of zebrafish to regenerate in epimorphosis.
REGENERASI SIRIP KAUDAL IKAN GATUL (Poecilia sp.) YANG MENGALAMI MALFORMASI Iza, Nikmatul; Listyorini, Dwi; Gofur, Abdul
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol 1 No 01 (2016): Edubiotik
Publisher : Program Studi Pendidikan Biologi, IKIP Budi Utomo Malang

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Abstract

Gatul fish in included in the family Poecilidae which is a small fresh water, male is smaller and more colorfull than female. This fish is easy to be taken care in laboratory and has a short reproduction cycle, thus allowing these fish to be one animal model development in the field of biology. Yet, in Indonesia the biological study upon Gatul fish is very limited. The aim of this study is to understand the process of caudal fin regeneration of Gatul fish are malformation (abnormal growth). Caudal fin of Gatul fish were vertically cut in a half of its length. Observation was done using 400x magnification. Picture with taken by camera with digital zooming. The subject of this study with a body length of 2-3 cm. The result shows that regeneration of Gatul caudal fin is started with wound healing in day-1 (D1= 24 hours after amputation) and blastema formation, then stump regeneration begin to grow in D2. Regenerative outgrowth begin in D3 characterized by the formation Blood island and fin rays. Differentiation begin in D6 and extended up to D18. From this research we can conclude that the process is going step by step through wound healing, stump growth, and tissue differentiation, and respectively
Varietas Padi Lokal Jawa Timur Tahan Cekaman Kekeringan Berdasarkan Gen DREB2A Lathif, Yudrik; Listyorini, Dwi; Suharti, Suharti
Biotropika: Journal of Tropical Biology Vol 6, No 3 (2018)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (340.57 KB) | DOI: 10.21776/ub.biotropika.2018.006.03.03

Abstract

Padi (Oryza sativa L.) adalah tanaman pangan utama masyarakat Indonesia. Masalah kekeringan dalam upaya penanaman padi adalah hal yang biasa di kalangan petani. Penelitian tahan cekaman kekeringan pada padi perlu dilakukan untuk mengatasi masalah ketersediaan pangan. Sifat tahan cekaman kekeringan pada tanaman salah satunya dikendalikan oleh gen DREB2A. Varietas padi lokal Indonesia untuk tahan cekaman kekeringan belum banyak diteliti. Penelitian ini bertujuan untuk mempelajari varietas padi lokal Jawa Timur yang tahan terhadap cekaman kekeringan menggunakan gen DREB2A, yang diisolasi menggunakan primer forward 5’-CCT CAT TGG GTC AGG AAG AA-3’ dan primer reverse 5’-GGA TCT CAG CCA CCC ACT TA-3’. Penelitian ini menggunakan tiga varietas padi lokal, yaitu Berlian dan SOJ A3, keduanya dari Banyuwangi, dan varietas Jawa dari Malang. Isolasi DNA total dilakukan mengikuti protokol Kit Macherey-Nagel. Amplifikasi gen DREB2A dilakukan menggunakan metode Polymerase Chain Reaction (PCR). Fragmen gen DREB2A berhasil diamplifikasi sepanjang 239 bp (varietas Berlian), 239 bp (varietas SOJ A3), dan 240 bp (varietas Jawa). Analisis sampel gen DREB2A varietas padi lokal Jawa Timur menunjukkan hilangnya kemampuan gen DREB2A dari varietas Jawa untuk mengikat cis-regulator elemen promotor gen target.
Identifikasi Padi Lokal Jawa Timur Berdasarkan Gen BADH2 yang Berpotensi sebagai Padi Beraroma Putra, Kurniawan Setia; Listyorini, Dwi; Suharti, Suharti
Biotropika: Journal of Tropical Biology Vol 6, No 3 (2018)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (307.922 KB) | DOI: 10.21776/ub.biotropika.2018.006.03.04

Abstract

Padi lokal merupakan padi liar yang telah dibudidayakan oleh petani Indonesia. Potensi padi lokal dapat digunakan sebagai plasma nutfah yang memiliki berbagai gen dalam mengendalikan sifat tertentu. Beberapa beras di Indonesia memiliki potensi sebagai beras aromatik. Berbagai varietas padi aromatik berpotensi meningkatkan kualitas beras di Indonesia. Aroma beras dikendalikan oleh gen betain aldehid dehidrogenase (BADH). Gen ini terdiri dari dua alel yaitu BADH1 dan BADH2, mengkodekan enzim yang bertanggung jawab untuk mengkatalisis sintesis senyawa 2-acetyl-1-pyrroline (2-AP). Penelitian ini bertujuan untuk mengidentifikasi potensi aroma varietas Berlian. Gen BADH2 diisolasi dari daun pada ketiga varietas tersebut dan diamplifikasi menggunakan primer forward 5'-TTGTTTGGCTCTTGCTGATG -3' dan primer reverse 5'CATAGGAGCAGCTGAAATATATACC-3'. Fragmen gen BADH2 berhasil diamplifikasi dengan ukuran 258 bp (varietas Berlian). Sekuen BADH2 varietas Berlian menunjukkan kemiripan 100% dengan sekuen BADH2 padi tidak beraroma dan tidak memiliki kemiripan dengan sekuen BADH2 varietas padi beraroma. Analisis potensi padi beraroma menunjukkan bahwa varietas Berlian tidak berpotensi sebagai padi beraroma.
AT3 (Acyltransferase) Gene Isolated From Capsicum frutescens cv. Cakra Hijau Habibi, Mohamad; Manggabarani, Andi Madhihah; Sulasmi, Eko Sri; Listyorini, Dwi
Journal of Tropical Life Science Vol 3, No 2 (2013)
Publisher : Journal of Tropical Life Science

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Abstract

Chili pepper is widely used and cultivated by Indonesian people. There are three species of chili pepper, i.e.: Capsicum annuum L., Capsicum frutescens L., and Capsicum violaceum HBK. Capsicum frutescens L. has a higher economic value due to its pungency and carotenoid content. C. frutescens has several cultivars, one of those is Capsicum frutescens cv. Cakra Hijau. This cultivar is resistant against pest and disease and has very high pungency. This special character of chili pepper is born by its secondary metabolic, Capsaicin. Moreover, capsaicin also serves as defense mechanism, antiarthritis, analgesic, and anticancer. This study aimed to isolate Acyltransferase (AT3) gene which encoding Capsaicin Synthase (CS) enzyme. AT3 gene was isolated through PCR using forward primer 5-ATG GCT TTT GCA TTA CCA TCA-3 and reverse primer 5-CCT TCA CAA TTA TTC GCC CA-3. Data were analyzed using DNA Baser, BLAST, and ClustalX. This study has successfully isolated 404 bp fragments of AT3 gene. This fragments located at 1918-1434 bp referred to AT3 gene from Capsicum frutescens cv. Shuanla. Isolation of upstream and downstream fragments of AT3 gene from Capsicum frutescens cv. Cakra Hijau is undergoing.
Morphological Study for Identification Improvement of Poeciliidae Family Based on Gonopodium structures and Morphometric analysis Rahayu, Dwi Anggorowati; Listyorini, Dwi; Ibrohim, Ibrohim
Journal of Tropical Life Science Vol 3, No 2 (2013)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.3.2.%x

Abstract

Poeciliidae family consists of small live-bearer fishes with sexual dimorphic. This fishes are widely distributed across Indonesian archipelago, but biological studies on this fish are very limited. This research is aimed to identify the species of Peciliidae fishes which lives in Sari Lake Pasuruan based on gonopodium structures and morphometric analysis. A specific characteristic of the Poeciliidae family is its gonopodium structures which is a modification of 3rd, 4th, and 5th rays of the males anal fin. Each species represented 50 samples male and female from each type we found and measured 26 characters (in mm). Cladogram based on morphometric measurements showed that sample 1 and 2 had 100% similarity, sample 3 had 94% similarity with sample 1 and 2. Sample 4 had 54% similarity with sample 1, 2, and 3. Based on gonopodium structures sample 1 and sample 2 included Xiphophorus hellerii Heckel possess large claw on the terminus of ray 5 hook is small crescent-moon shaped on 3rd ray and lacking of gonopodium palp, sample 3 possesses distal platform with 11 retrosse serrae,and membranous hook so we were able to identify the sample 3 as Poecilia mexicana Steindachner, whilst sample 4 was Poecilia reticulata Peter had a long-pointed comb-like spines on ray 3rd and and has a small hook on the tip of 5p ray.
Observation of Frog Species in State University of Malang as a Preliminary Effort on Frog Conservation Wulandari, Dian Ratri; Habibi, Muhammad; Listyorini, Dwi
Journal of Tropical Life Science Vol 3, No 1 (2013)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.3.1.%x

Abstract

Frog is an amphibian which widely distributed around the world. Indonesia houses 450 species which represent 11% of frog species in the world. In Java Island living 42 species of frogs and toads. Frogs can be used as an environment indicator due to the presence of frog in a particular place indicates that the place is stay natural and unpolluted. State University of Malang Campus #1 which is located in the heart of Malang District has been developing rapidly, currently. Thus, it requires for the construction of new various facilities to support it widen activities. Extensive construction can be destructive even damaging to the habitat of frog, which potentially threat the frog life, if it does not take a consideration on the environmental impact, carefully. This study aimed to identify the species of frog which is survives in State University of Malang with consideration of the frog species found in 1995. Species identification conducted by observing the morphologicalcharacter. This study found four species with three species which are survive and as found in 1995; those are Duttaphrynus melanostictus, Polypedates leucomystax, and Kaloula baleta; and one new species that is Rana chalconota. This study also reveals there are four species which are extinct; those are Fejervarya cancrivora, Fejervarya limnocharis, Ingerophrynus biporcatus, and Occidoziga lima. This situation shows the decreasing amount of species from 7 to 4 within 17 years. This result indicates that there is a serious environmental degradation which caused the losing of frog habitats. Further research is needed to study the ecological condition changing in order to save the frog species.
Embryo Development of Tree Frog Polypedates leucomystax at Campus of State University of Malang Peralindah, Pearlindah; Kusumawati, Emy; Wulandari, Dian Ratri; Listyorini, Dwi
Journal of Tropical Life Science Vol 2, No 3 (2012)
Publisher : Journal of Tropical Life Science

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Abstract

Tree frogs live in natural places which are unpolluted. Regarding their role as an ecological indicator, the decrease of frogs population in a particular habitat indicates the danger of environment quality decrease. Moreover, this condition can harm the frogs themselves. All kinds of frogs breed in aqueous environment such as ponds, marshes, and farming fields. One of the tree frogs, Polypedates leucomystax, which belongs to Familia Rachophoridae, is widely spread in Indonesia. This frog has yellowish brown skin with black spots or six lines extending from head to the posterior tip of body. A breeding couple of the frog produces foam nests on the water or plants around water body, where they will nest their fertilized eggs. This species produces over a hundred embryos in one spawning season. These embryos require appropriate conditions to develop normally in the nature. Frog embryo development may becomes a reference to understand how the frog population survives. This study focused on P. leucomystax with regards to its decrease in number due to the drying up of the environment and a lot lost of trees in Campus of State University of Malang. The development of P. leucomystax embryos in the reproduction foam was observed until it reached a tadpole stage. The result showed that the embryos developed in the foam until they hatched then they move out of the foam into the water under which they would continue their development. Considering that water body is a critical requirement for the development of P. leucomystax embryos, it is our responsibility to make any efforts to conserve not only the trees but also any type of water bodies including ponds, marshes, and farming fields as well. Keywords: embryo development, Polypedates leucomystax, Tree frog conservation
Berlian, SOJ A3 and Jawa Local Rice Varieties Phylogenetically Distinct From Oryza sativa Indica and Oryza sativa Japonica Lathif, Yudrik; Retnaningtyas, Riri Wiyanti; Listyorini, Dwi; Suharti, Suharti
El-Hayah : Jurnal Biologi Vol 6, No 4 (2018): EL-HAYAH (VOL 6, NO 4,March 2018)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v6i4.5883

Abstract

The genetic resources identification of Indonesian local rice varieties is a crucial work should be done to conserve our native germplasm. This research aimed to know the taxonomical position of East Java local rice varieties including Jawa (JW), Berlian (BR), and SOJ A3 (SJ) using DNA barcode based on rbcL gene. Total DNA of each sample was isolated from leaves. A pair of forward 5'-ATG TCA CCA CAA ACA SJA AC-3' and reverse 5'-TCG GTA CCT GCA GTA GC-3' primers were used to amplify fragments of rbcL gene resulting in 751bp, 755bp, and 754bp fragments from BR, SJ, and JW varieties, respectively. Phylogenetic tree reconstruction revealed that our three local varieties were forming a cluster separated from the widely cultivated subspecies Oryza sativa Indica and Oryza sativa Japonica. However, further studies are necessary to reveal a more precise position of the local varieties in a phylogenetic tree on the species level.
Identification of Genetic Relationship of Local Rice in East Java Based on gene matK Putra, Kurniawan Setia; Listyorini, Dwi; Suharti, Suharti
El-Hayah : Jurnal Biologi Vol 6, No 4 (2018): EL-HAYAH (VOL 6, NO 4,March 2018)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v6i4.5578

Abstract

Genetic diversity in living things is very important in the fulfillment of germplasm and conservation activities. East Java local rice is not so much cultivated farmers, so that the existence of local rice has begun to be replaced with new varieties and it is feared will happen genetic erosion of local rice. The purpose of this study to identify the genetic relationships of local rice and phylogenetic position of the rice contained in the Gene Bank. The method used to identify a molecular genetic using short pieces of DNA called DNA barcode. The results of this study indicate that the three local varieties of Berlian (Br), Genjah Harum (Gh) and Jawa (Jw) varieties can be identified by the matK gene barcode. The results of electrophoresis visualization showed that the DNA bands of the three samples were 900 bp. The results of DNA BLAST analysis show that the genetic relationships level with Oryza sativa and Oryza rufipogon is 100%. The results of the phylogenetic tree analysis showed that East Java's local rice was in one taxa with other rice and had a confidence level above 50
Co-Authors Abdul Ghofur Abdul Gofur Abdul Gofur Achmad Rodiansyah Adi Kus Rochman Jk Afrabiyas Firyal Hanasepti Agung Wibowo Agung Witjoro Ainul Fitria Mahmudah Aisyah Hidayati Algafari Bakti Manggara Alvian Guntur Prasetya Aziz Anastacia Verena Salim Andi Madhihah Manggabarani Anggun Sari Anjarwati Annasa Sabatia Ardana, I Kade Karisma Gita Asiah Muchtar Atik Kurniawati Bani Sudardi Choirun Nita Fikriani Choirun Nita Fikriani Cicik Tri Jayanti Dahlia Dahlia Dahlia Dahlia Delia Wahyu Pangesti Dermawan, Taufik Desy Irafadillah Effendi Dian Ratri Wulandari Didin Widyartono Dita Tri Mulyani Djoko Saryono Dwi Anggorowati Rahayu Dwi Anggorowati Rahayu Dwi Anggorowati Rahayu Dwi Anggorowati Rahayu Dwita Novitasari Dyati Galuh Pratita Eko Agus Suyono Eko Sri Sulasmi Elhah Nailul Khasna Emilia Umrotin Emy Kusumawati Endang Suarsini Endik Deni Nugroho Evi Susanti Fatchur Rohman Fikriani, Choirun Nita Firda Ama Zulfia Fitri Wahyuni Wahyuni Galina Istiqhfarini Gracela Noveline Christiana Telaumbanua Habibi, Mohamad Hadi Sowono Hadi Suwono Helmi Muzaki Hendra Susanto Herawati Susilo Herdina Rahma Zalita I Kade Karisma Gita Ardana I Kade Karisma Gita Ardana I Kade Karisma Gita Ardana Ibrohim Iin Murtini Indra Kurniawan Saputra Intan Chairun Nisa Iza, Nikmatul Izdihara Arini Aulia Jeni Jeni Jiilan Hani Safitri Ken Raos Regina Kifan Wigrahanto Kurniawan Setia Putra Kusubakti Andajani Kusumawati, Emy Lathif, Yudrik Lathif, Yudrik Lea Assyifa M. Nidhamul Maulana Manggabarani, Andi Madhihah Mar'atus Sholihah Mastika Marisahani Ulfah Mohamad Habibi Muh Ade Artasasta Muhammad Habibi Muhibbatul Ma'rifah Murni Sapta Sari Muthia Naila Mazieda Naailatu Nur Azizah Ndzani Latifatur Rofi’ah Nikmatul Iza Nila Kartika Sari Nina Bunga Anggraini Nina Bunga Anggraini Ningsih, Husdiani Norman Yoshi Haryono Nur Hasbi Nurul Annisa Putri Pearlindah Peralindah Peralindah, Pearlindah Putra, Kurniawan Setia Putra, Kurniawan Setia Radhiyah, Rizqa Rahmi Masita Rani Nuzulul Laili Ratna Juwita Rayhan Rizky Fadhillah Reeno Al Hikmatus Sholekah Reynaldi Januar Hudayanto Rina Wahyuningsih Riri Wiyanti Retnaningtyas Riri Wiyanti Retnaningtyas Riskha Anggita Endriawati Rizki Nurul Prasetyani Rizqa Radhiyah Rizqullah Janitra Kristian Abadi Rohmawati, Uun Rozana, Kennis Sari, Nila Kartika Sindy Firnanda Siti Zubaidah Sitoresmi Prabaningtyas Sri Sukaesih Suharti Suharti Suharti Suharti Suharti Sukiman Sunarmi Susriyati Mahanal Tita Putri Milasari Tutut Indria Permana Utami Sri Hastuti Wahyudi Siswanto Widia Tamara Winda Dwi Astuti Windri Hermadhiyanti Wisnubroto Wisnubroto Wisnubroto, Wisnubroto Wulandari, Dian Ratri Yessi Affriyenni Yudrik Lathif Zaäfri A Husodo Zidan Aqillah Rasyid ‘Ainun Sayyidah Zakiyah