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All Journal PREVENIRE: Journal of Multidisciplinary Science Consilium Sanitatis: Journal of Health Science and Policy
Fera Aulia
Universitas Negeri Yogyakarta
Religion Arts Humanities Computer Science & IT Dentistry Economics, Econometrics & Finance Education Engineering Health Professions Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences

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16S Metagenomics Of Branch-Leaf Compost At The Expo Memorial Park Osaka: 16S Metagenomik Kompos Cabang-Daun Di The Expo Memorial Park Osaka Fera Aulia; Risma Wiharyanti; Karan Lohmaneeratana
Journal of Multidisciplinary Science Vol. 2 No. 2 (2023): Maret-April
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Institut Studi Islam Sunan Doe

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58330/prevenire.v2i2.187

Abstract

Compost is one of the potential microbial habitats for the discovery of a wide range of novel biocatalysts. Compost sample originally made of plant litter, were collected at the Expo Park (Osaka, Japan). The temperature of composts is around 50 °C. Metagenomic DNA was isolated from compost and was used to amplify partial 16S rRNA genes (rDNAs) by polymerase chain reaction for investigation of the microbial diversity in the compost. Molecular microbial identification was carried out by metagenome analysis of 16S rRNA using MinION Next Generation Sequencing (NGS). The results indicated that branch-leaf compost sample from Expo Park is dominated by thermophilic microorganism. The majority of bacteria are Caldicoprobacter, Hungateiclostridium, and Thermoclostridium. To conclude, thermophilic microorganism is not only found in extreme environment, they also presence even in moderate temperature.
Desain Primer dan Amplifikasi Gen PhtD dari Streptococcus pneumoniae secara in silico Sebagai Kandidat Vaksin Pneumonia Independent Serotype Berbasis Protein Risma Wiharyanti; Fera Aulia; Astiningdiah Merdekawati
Consilium Sanitatis: Journal of Health Science and Policy Vol. 1 No. 2 (2023): April
Publisher : EDUPEDIA Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (636.629 KB) | DOI: 10.56855/jhsp.v1i2.283

Abstract

Purpose: The aim of this study was to obtain a primer design capable of amplifying the PhtD gene from various S. pneumoniae bacterial serotypes as a protein-based pneumonia vaccine candidate that does not depend on bacterial serotypes.Methods: Primary preparation was carried out through a bioinformatics approach using several software followed by testing the use of primers in the amplification process which was carried out in silico.Results: The results of this study were a pair of primers capable of amplifying the PhtD gene from several S. pneumoniae serotypes.Conclusion: The PhtD gene of the 6 serotypes of S. pneumoniae has high similarities, making it suitable for use as a protein-based vaccine candidate. Based on the consensus of the six serotypes, a pair of primers was obtained that were able to amplify 17 S. pneumoniae strains from several serotypes contained in the reference sequence in silico.
Co-Authors Astiningdiah Merdekawati Karan Lohmaneeratana Risma Wiharyanti