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All Journal PREVENIRE: Journal of Multidisciplinary Science
Karan Lohmaneeratana
Kasetsart University
Religion Arts Humanities Computer Science & IT Economics, Econometrics & Finance Education Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences

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16S Metagenomics Of Branch-Leaf Compost At The Expo Memorial Park Osaka: 16S Metagenomik Kompos Cabang-Daun Di The Expo Memorial Park Osaka Fera Aulia; Risma Wiharyanti; Karan Lohmaneeratana
Journal of Multidisciplinary Science Vol. 2 No. 2 (2023): Maret-April
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Institut Studi Islam Sunan Doe

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58330/prevenire.v2i2.187

Abstract

Compost is one of the potential microbial habitats for the discovery of a wide range of novel biocatalysts. Compost sample originally made of plant litter, were collected at the Expo Park (Osaka, Japan). The temperature of composts is around 50 °C. Metagenomic DNA was isolated from compost and was used to amplify partial 16S rRNA genes (rDNAs) by polymerase chain reaction for investigation of the microbial diversity in the compost. Molecular microbial identification was carried out by metagenome analysis of 16S rRNA using MinION Next Generation Sequencing (NGS). The results indicated that branch-leaf compost sample from Expo Park is dominated by thermophilic microorganism. The majority of bacteria are Caldicoprobacter, Hungateiclostridium, and Thermoclostridium. To conclude, thermophilic microorganism is not only found in extreme environment, they also presence even in moderate temperature.
Co-Authors Fera Aulia Risma Wiharyanti