Claim Missing Document
Check
Articles

Found 8 Documents
Search

Red fruit oil increases trophoblast cells and decreases caspase-9 expression in placenta of lead exposed mice Sumarsono, Portia; Widjiati, Widjiati; Susilowati, Suherni
Universa Medicina Vol 35, No 2 (2016)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2016.v35.110-118

Abstract

BACKGROUNDLead is able to pass through the placental barrier and interfere with fetal development. Red fruit has high antioxidant activity, due to carotenoids (pro-vitamin A), tocopherols (vitamin E) and unsaturated fatty acids. This study aims to examine the effect of red fruit oil towards placenta in pregnant mice before lead exposure.METHODSThis was a laboratory experimental post test only study, using 20 pregnant mice (Mus musculus) that were randomized into five groups. The negative control group was not exposed to lead; intervention group 0 (P0) was exposed to lead at 0.011 mg/20 g BW/day on days 6-15 of gestation. The intervention groups P1-P3 were given red fruit orally at 0.3 mL/20 g BW/day, 0.8 mL/20 g BW/day, 0.9 mL/20 g BW/day, respectively, before lead exposure. Normal trophoblast cell count and caspase-9 expression of trophoblasts were calculated. One-way ANOVA, Kruskal-Wallis and Mann Whitney tests were used to analyze the data.RESULTSThe intervention groups P2 and P3 given red fruit at 0.8 mL/20 g BW/day and 0.9 mL/20 g BW/day showed a significant increase in normal trophoblast cell count of 88.2% (p=0.024) and a decrease in caspase-9 expression of 70.8% compared with P0 to which was not given red fruit (p=0.020). CONCLUSIONRed fruit increased normal trophoblast cell count by 88.2% and decreased caspase-9 expression by 70.8% in pregnant mice before lead exposure. It should be noted that red fuit may prevent oxidative damage to cells.
MOZART MUSIC INCREASES THE NUMBER OF GLIAL CELLS COMPARED TO INDONESIA POP AND RELIGIOUS MUSIC Rahayu, Pipit Sri Estuning; Hermanto, Hermanto; Widjiati, Widjiati
Saintika Medika: Jurnal Ilmu Kesehatan dan Kedokteran Keluarga Vol 15, No 2 (2019): December 2019
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (218.975 KB) | DOI: 10.22219/sm.Vol15.SMUMM2.10265

Abstract

Music stimulation is an important component for prenatal fetal development. Both pop and religious music are easy to listen and widely accepted in Indonesia. This study was to analyze the effect of Mozart, pop, and religious music exposure during pregnancy to the number of glial cells in the brain of Rattus norvegicus offspring. The samples were divided into three groups based on the exposure for each group, namely Mozart, pop, and religious music, duration of 60 minutes with 65dB intensity, initiated on the 10th day of pregnancy for 9 days in the soundproof chamber. Three brains of the offsprings were dissected and prepared for Hematoxylin-Eosin staining counted on 5 fields of view and 400 magnification strength.Different glial cells number of Rattus norvegicus brain between groups were observed. Mozart music (28,29) showed a highest mean and pop music (18,67)  showed the lowest mean. Significant difference of the number of brain glial cells between Mozart music compared to pop and religious music groups were observed, with p value <0,005.The number of brain glial cells of Rattus norvegicus offsprings in the Mozart group were significantly higher than those in pop and religious groups.
Effects of Curcumin on Vascular Endothelial Growth Factor Expression on Rattus norvegicus Cervical Cancer Xenograft Model Muninggar, Laili; Widjiati, Widjiati; Yuliati, Indra; Askandar, Brahmana; Hartono, Poedjo
Indonesian Journal of Cancer Vol 12, No 3 (2018): July-September
Publisher : National Cancer Center - Dharmais Cancer Hospital

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (907.462 KB) | DOI: 10.33371/ijoc.v12i3.616

Abstract

Objective: To analyze the effect of curcumin in VEGF expression on Rattus norvegicus cervical cancer cell xenograft model.Methods: An experimental study with randomized post test only control group design. The subjects were Rattus norvegicus (Sprague Dawley), inoculated with He-la cervical cancer cells from American Type Culture Collection (ATCC) processed in stem cell laboratory Institute of Tropical Disease (ITD) Airlangga University. 5x106 of He-La cells were injected subcutaneously in dorsal flank area of Rattus norvegicus. After 30 days of observation we performed histopathological examination of xenograft tissue and randomized into 2 groups which were given curcumin orally 1000 mg/kg (curcumin group) vs. no therapy (control group). After another 30 days the xenograft tissue was dissected and underwent immunochemistry examination for VEGF expression.Results: 32 samples of Rattus norvegicus were divided into 2 groups, In curcumin group the VEGF median expression was 2,2 (0,3-7,6) and in control group the VEGF median expression was 6,6 (1,2-12). There was a statistically significant difference with p value =0,009 with Mann Whitney test (p<0,05).Conclusion: VEGF expression in Rattus norvegicus xenograft model of cervical cancer was suppressed by giving Curcumin 1000 mg/kgBB orally.
Synapsis Expression Increased By Mozart’s Music Stimulation Compared With Indonesian Pop And Religious Music Amila, Ukhti Mukminah ilmi; Joewono, Hermanto Tri; Widjiati, Widjiati
Saintika Medika: Jurnal Ilmu Kesehatan dan Kedokteran Keluarga Vol 16, No 1 (2020): June 2020
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (180.513 KB) | DOI: 10.22219/sm.Vol16.SMUMM1.10784

Abstract

Classical music stimulation is a proven method that affect intelligence as well as brain function. Mozart’s classical music is classical music that could stimulate the growth of synaptic networks. To analyze the difference of synapsin expression of the brain of Rattus norvegicus  offspring on Mozart’s, pop, religious and without music stimulation during pregnancy. Experimental. Treatments were subjected to Rattus norvegicusas a subject organism, initiated on the 10th day of pregnancy, in 1 hour duration in a soundproof chamber, intensity of 65 dB. On the 19th day of pregnancy the subjects were sacrificed and three offsprings were chosen. The head of the chosen Rattus norvegicusoffsprings were decapitated and the brain subsequently sectioned to calculate synapsin expression by immunohistochemistry method with 400 magnification strength microscope on the 5 field of view. Significant difference of synapsin expression of the brain of Rattus norvegicus offspring was observed between Mozart’s and control group. Significant differences of synapsin expressions were observed in the cerebellum of the newly born Rattus novergicus between Mozart and pop music groups (p = 0,007), Mozart and religious music groups (p = 0,008), Mozart and without music groups (p = 0,004).Synapsin expression of Rattus norvegicus brain offsprings were higher in Mozart music stimulation compared with pop and religious music stimulation
PERBEDAAN PENGARUH PAPARAN MUSIK MOZART, BEETHOVEN, DAN CHOPIN SELAMA KEBUNTINGAN TERHADAP KEPADATAN DENDRIT DI CEREBELLUM Rattus norvegicus BARU LAHIR Amishinta, Ancha Ayu; Joewono, Hermanto Tri; Widjiati, widjiati
JURNAL ILMIAH OBSGIN : Jurnal Ilmiah Ilmu Kebidanan & Kandungan P-ISSN : 1979-3340 e-ISSN : 2685-7987 Vol 10 No 2 (2018): SEPTEMBER
Publisher : NHM PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Indonesia is categorized as middle humandevelopment index. One of the causes is the fluentignorance of brain growth and development during1000 days of life period. This period is the bestopportunity to give proper stimulations to increase themaximal brain growth and development. Exposure toclassical music such as Mozart, Beethoven, andChopin music can increase the wave of brain activity.During pregnancy, Mozart music is proven to increasethe dendritic density. Analyzing the difference ofdendritic density of cerebrum and cerebellum ofnewborn baby of Rattus norvegicus that are exposedto the Mozart, Beethoven, and Chopin music, and thatis not exposed to music during pregnancy. Laboratoryexperimental research, posttest-only control groupdesign. Subjects were female pregnant Rattusnorvegicus, grouped into 4 random groups: 1 controlgroup and 3 treatments groups; with 6 samples each.Subjects were super ovulated, and 65 dB intensity ofmusic was played for an hour at 20.00-21.00 on thesubjects on 10th day of pregnancy. On 20th day ofpregnancy, the mothers were dissected usingcaesarean section. 2 heaviest newborn babies ofRattus norvegicus were taken, and their brain tissueswere taken as samples. The dendritic density ofcerebrum and cerebellum were observed using GolgiCox method of silver impregnationprocedure.Statistical test concluded there weresignificant differences of dendritic density of cerebrumand cerebellum of newborn babies of Rattusnorvegicus among the exposure to Mozart,Beethoven, and Chopin music, and without theexposure to music with value of p=0,004 (<0,05) incerebrum and p=0,003 (<0,05) in cerebellum. Thedendritic density of cerebrum and cerebellum ofnewborn babies of Rattus norvegicus that wereexposed to Mozart music during pregnancy werehigher than that are exposed to Beethoven andChopin music and that were not exposed to music. Itis recommended for the next research to do furtherresearch to prove the dendritic density caused by themusical stimulation during pregnancy when Rattusnorvegicus grows up.
PERBEDAAN PENGARUH PAPARAN MUSIK MOZART, BEETHOVEN, DAN CHOPIN SELAMA KEBUNTINGAN TERHADAP INDEKS APOPTOSIS SEL NEURON DI CEREBELLUM Rattus norvegicus BARU LAHIR Fajrin, Dessy Hidayati; Joewono, Hermanto Tri; Widjiati, widjiati
JURNAL ILMIAH OBSGIN : Jurnal Ilmiah Ilmu Kebidanan & Kandungan P-ISSN : 1979-3340 e-ISSN : 2685-7987 Vol 10 No 2 (2018): SEPTEMBER
Publisher : NHM PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The efforts that can be done to improvebrain cells in the prenatal period is the provision ofnutrients and good stimulation. Exposure to classicalmusic such as Mozart, Beethoven, and Chopinmusic can increase the wave of brain activity. Duringpregnancy, Mozart music is proven to decrease theapoptosis of neuronal.Analyzing the difference of apoptosisneuronal of cerebrum and cerebellum of newbornbaby of Rattus norvegicus that are exposed to theMozart, Beethoven, and Chopin music, and that isnot exposed to music during pregnancy.Laboratory experimental research, posttestonly control group design. Subjects were femalepregnant Rattus norvegicus, grouped into 4 randomgroups: 1 control group and 3 treatments groups;with 6 samples each. Subjects were super ovulated,and 65 dB intensity of music is played for an hour at20.00-21.00 on the subjects on 10th day ofpregnancy. On 20th day of pregnancy, the motherswas dissected using SC technique. 2 heaviestnewborn babies of Rattus norvegicus were taken.Statistical test concluded there wassignificant differences of apoptosis neuronal ofcerebellum of newborn babies of Rattus norvegicusamong the exposure to Mozart, Beethoven, andChopin music, and without the exposure to musicwith value of p=0,033 in cerebellum.In conclution, the exposure of Mozart’smusic during pregnancy perform expression ofBDNF cerebellum in the offspring-rat was higherthan exposed Beethoven’s music, Chopin and notexposed to music.
PERBEDAAN PENGARUH PAPARAN MUSIK MOZART, BEETHOVEN DAN CHOPIN SELAMA KEBUNTINGAN TERHADAP JUMLAH SEL NEURON DI CEREBELLUM Rattus norvegicus BARU LAHIR N, Herlina Alvianti; Joewono, Hermanto Tri; Widjiati, widjiati
JURNAL ILMIAH OBSGIN : Jurnal Ilmiah Ilmu Kebidanan & Kandungan P-ISSN : 1979-3340 e-ISSN : 2685-7987 Vol 10 No 2 (2018): SEPTEMBER
Publisher : NHM PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Efforts could be made to improve humanresources that have intelligence required apositive relationship between health and goodeducation quality, one of the efforts to improvehuman resources requires good brain qualitysince the phase of conception. Classical musichas been shown to boost brain function andhuman intellectual optimally believed to have thebest stimulating effect on babies. Cellularexposure to Mozart affects the number of moreneuronal cells. Analyze the number of cerebrumand cerebellum neuronal cells of newborn Rattusnorvegicus between the exposed to Mozart music,Beethoven, Chopin and not exposed to musicduring pregnancy. This research was anexperimental research with posttest only controlgroup design. The sample was divided into fourgroups randomly, non-exposed, Mozart musicexposure group, Beethoven music exposuregroup, Chopin music exposure group, exposed for1 hour during the night after Rattus norvegicuswas pregnant on day-10 with an intensity of 65 dBand a distance of 37 cm from the rat cage. On the20th day of the pregnant mother of Rattusnorvegicus was sacrificed and selected two ofRattus norvegicus's babies with the greatestweight and then the brains of Rattus norvegicus'sbabies were decapitated and brain dissection tocount the number of neuronal cells withHemotoxyln-Eosin staining. The statistical resultsshowed that the number of neurons of cerebellumcells in the Mozart group differed significantly fromBeethoven, Chopin and not exposed to music withp <0.05. The number of neuronal cells ofcerebellum of the newborn Rattus norvegicus whoexposed to Mozart music during pregnancyproved higher than that exposed to Beethovenmusic, Chopin and not exposed to music.
PERBEDAAN PENGARUH PAPARAN MUSIK MOZART, BEETHOVEN DAN CHOPIN SELAMA KEBUNTINGAN TERHADAP EKSPRESI BRAIN DERIVED NEUROTROPHIC FACTOR DI CREBELLUM RATTUS NORVEGICUS BARU LAHIR Permatasari, Pitria; Joewono, Hermanto Tri; Widjiati, widjiati
JURNAL ILMIAH OBSGIN : Jurnal Ilmiah Ilmu Kebidanan & Kandungan P-ISSN : 1979-3340 e-ISSN : 2685-7987 Vol 10 No 2 (2018): SEPTEMBER
Publisher : NHM PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

r 2015 was ranked 113 out of 188 countries.Education would be needed to raise HDI’s rank. Oneprinciple component of education is the quality of thebrain. BDNF expression in the brain was affected bymany stimuli. Mozart classical music had aneurobiological effect which had ability to increaseBDNF. Whereas, other classical musics such asBeethoven and Chopin had never been studied. Thisstudy aims to cerebellum’s BDNF expression in theoffspring of Rattus norvegicus that ere exposed tothe Mozart , Beethoven, Chopin during pregnancy.The method used is pure laboratoryexperiment with posttest only control group design.The variables measured were BDNF expression inthe brain. The samples were divided into 4 groups ofeach 6 rats.The results showed that significantdifferences in BDNF expressions between controlgroup and Mozart (p=0,023; p<0,05), betweenMozart group and Chopin (p=0,003). There were nostatistically detected differences between controlgroup and Beethoven (p=0,256), control group andChopin (p=0,684), Mozart group and Beethoven(p=0,092) and Beethoven group and Chopin(p=0,292). Based on the above results it can beconcluded that the exposure of Mozart’s musicduring pregnancy had a higher expression of BDNFin the offspring-rat’s cerebellum compare to thosewhich exposed by Beethoven and Chopin’s music.