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Lactobacillus bulgaricus Sebagai Probiotik Guna Peningkatan Kualitas Ampas Tahu Untuk Pakan Cacing Tanah Purkan, Purkan
Jurnal Kimia Riset Vol 2, No 1 (2017): Juni
Publisher : Universitas Airlangga

AbstrakPenelitian ini bertujuan untuk menentukan aktivitas protease dari probiotik Lactobacillus bulgaricus dan pengaruh probiotik Lactobacillus bulgaricus dalam fermentasi pakan ampas tahu untuk meningkatkan produktivitas cacing tanah. Metode yang digunakan untuk penentuan aktivitas protease dalam hidrolisis substrat kasein adalah metode Bradford. Dari hasil penelitian, probiotik Lactobacillus bulgaricus mengeluarkan protease selama 18 jam pertumbuhan, dengan aktivitas protease sebesar 131,04 U/mL. Probiotik Lactobacillus bulgaricus OD 0,6 dapat menghidrolisis protein ampas tahu sebesar 1,48 µg/mL dalam 12 jam fermentasi. Produktivitas cacing tanah mengalami peningkatan berat cacing tanah karena adanya pengaruh probiotik Lactobacillus bulgaricus pada pakan ampas tahu yang ditunjukkan dengan persen kenaikan berat cacing tanah sebesar 32,13%.Kata kunci: Probiotik, Lactobacillus bulgaricus, Enzim protease, Cacing tanah

PRODUKSI ENZIM KITINASE DARI Aspergillus niger MENGGUNAKAN LIMBAH CANGKANG RAJUNGAN SEBAGAI INDUSER Purkan, Purkan; Baktir, Afaf; Sayyidah, Arju Rohmah
Jurnal Kimia Riset Vol 1, No 1 (2016): Juni
Publisher : Universitas Airlangga

AbstrakKitinase merupakan enzim hidrolitik yang dapat menghidrolisis kitin pada ikatan Î²-1,4-glikosidiknya dengan menghasilkan derivat-derivat kitin seperti oligomer kitin yang mempunyai banyak manfaat. Penelitian ini bertujuan untuk melakukan pengembangan produksi enzim kitinase dari sumber lokal yang melimpah di alamserta murah dengan melakukan optimasi substrat dalam hal ini digunakan substrat tetes tebu (molase) dan limbah cangkang rajungan untuk produksi enzim kitinase dari Aspergillus niger. Sebelumnya, dilakukan kultivasi isolat kapang Aspergillus niger dengan membuat kurva pertumbuhan menggunakan metode masa sel kering dimana dari hasil penelitian inokulasi optimal adalah 22 jam. Pada proses produksi, diperoleh waktu fermentasi optimal adalah 52 jam dengan menentukan uji aktivitasnya menggunakan metode turbidimetri. Hasil optimasi substrat menunjukkan bahwa enzim kitinase yang maksimal diperoleh pada penambahan molase 0,5% (b/v) dengan unit aktivitas enzim 0,14726 (U/mL) dan cangkang rajungan 2% (b/v) dengan unit aktivitas enzim yang dihasilkan 0,12826 (U/mL). Kitinase dari Aspergillus niger ini mempunyai pH optimal 6 dan suhu optimal 40 oC.Â Kata kunci: Aspergillus niger, kitinase, cangkang rajungan, molase Â Â AbstractChitinase is a hydrolytic enzyme that hydrolyzes chitin on Î²-1,4-glycosidic bond and thereby producing chitin derivatives such as chitin oligomers that have multiple benefits. The purpose of this research was to develop the production of chitinase enzyme from cheap and are abundant local nature sources, by optimizations substrate in this case the substrate used molasses and crab shell waste for the production of chitinase enzyme from Aspergillus niger. Previously, isolates of Aspergillus niger cultivated by creating a growth curve using dry cell mass method which from the results of research inoculation optimal are 22 hours. In the production process, obtained the optimum fermentation time is 52 hours to determine the activity test using turbidimetry method. Result of substrate optimizations indicate that chitinase enzyme maximum by addition of molasses obtained in 0.5% (w/v) with enzyme activity units 0.14726 (U/mL) and crab shells 2% (w/v) with enzyme activity units 0.12826 (U/mL). Chitinase from Aspergillus niger has a pH optimum 6 and temperature optimum 40 oC.Â Keywords: Aspergillus niger, chitinase, crab shells, molasses

Pencirian Produksi Amilase oleh Saccaromyces cerevisiae W303A Rekombinan Thontowi, Ahmad; Puspaningsih, Ni Nyoman Tri; Hadi, Sofjan; Purkan, Purkan; Irawan, Bambang
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTCharacterization of Amylase Production by Saccharomyces cerevisiae W303A Recombinants. Cloning of amylase gene from Endomycopsis fibuligera ITB.R.cc.64 into S. cerevisiae W303a can effectively increase the yeast function to digest starch directly into ethanol. Production of amylase by S. cerevisiae W303a recombinants (I and P) were done by growing in yeast peptone starch (YPS) medium. The result showed that the recombinants could be produced of amylase by gave clear zone after staining by iodium vapor. The optimum condition of production of amylase by S. cerevisiae W303a recombinants were pH 7.0, 40?C temperature incubation, and gave maximum activity after 36 hours incubation. Amylase activity of I was higher than P recombinant for these condition respectively.Key words: Characterization, amylase, S. cerevisiae W303a

REPARASI DAN KARAKTERISASI ALFA-Fe2O3 /ZEOLIT Y UNTUK REAKSI PERENGKAHAN ASAM PALMITAT Abdulloh, Abdulloh; Purkan, Purkan; Hardiansyah, Nizar
Jurnal Kimia Riset Vol 2, No 2 (2017): Desember
Publisher : Universitas Airlangga

Telah dilakukan preparasi dan karakterisasi a-Fe2O3/zeolite Y sebagai katalis uji reaksi perengkahan asam palmitat. Zeolit A disintesis dari NaOH, NaAlO2 dan TEOS dengan metode kristalisasi bertahap. Katalis alfa-Fe2O3/zeolite Y disiapkan melalui metode impregnasi menggunakan larutan Fe(NO3)3Â 1 M dan proses kalsinasi pada suhu 500 oC dengan dialiri gas N2 selama 5 jam. Katalis dikarakterisasi dengan XRD, FTIR dan surface area analyzer menggunakan metode BET. Uji aktivitas katalis dilakukan pada suhu 380 â€“ 400 oC. Analisis GC-MS terhadap hasil reaksi perengkahan menunjukkan terbentuknya senyawa-senyawa hidrokarbon seperti alkana dan alkena yang memiliki rantai karbon lebih pendek dari asam palmitat atau biogasoline

Production of Cellulase Enzyme from Aspergilus niger using Rice Husk and Bagasse as Inducer Purkan, Purkan; Purnama, HD; Sumarsih, S
Jurnal ILMU DASAR Vol 16 No 2 (2015)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Aspergillus niger is fungi can produce cellulase enzyme with agriculture waste as natural inducers. The purpose of this study was to compare the natural inducers potential between rice husk and bagasse to produce cellulase enzyme from Aspergillus niger. Production of cellulase enzyme was done with variety of inducers such as CMC, rice husk, and bagasse. The optimization of enzyme production includes optimum production time, inducer type, and optimum concentration of inducer. Furthermore, the enzyme also was characterized in pH and temperature. Enzyme activity test using the DNS method with CMC as substrate. According of this test result show that highest cellulase enzyme activity has production time for 108 hours with rice husk as inducer. The optimum rice husk concentration was needed of 2.5%. The cellulase enzyme was induced by rice husk has optimum activity at pH 4 and 50°C of 0.709 IU/mL. Keywords : cellulase enzymes, Aspergillus niger, inducers, rice husk, bagasse.

RESISTANCE LEVEL OF Pseudomonas stutzeri AGAINST MERCURY AND ITS ABILITY IN PRODUCTION OF MERCURY REDUCTASE ENZYME Purkan, Purkan; Nurmalyya, Safita; Hadi, Sofijan
Molekul Vol 11, No 2 (2016)
Publisher : Universitas Jenderal Soedirman

Mercury reductase is an enzyme that is able to reduce Hg2+ to Hg0 non toxic. This enzyme is usually produced by mercury resistant bacteria. The research wanted to determine the resistance of indigenous Pseudomonas stutzeri isolate toward mercury and to explore the mercury reductase activity which is produced by the bacteria. The results of resistance assay of the Pseudomonas stutzeri toward mercury ion showed that the isolate could survive in media containing HgCl2 up to a concentration of 80 µM. The bacteria could produce mercury reductase optimally at the 24th of fermentation time. The enzyme showed optimum activity at pH 7 and temperature of 45 oC

EKSPLORASI BAKTERI KITINOLITIK DARI SAMPAH ORGANIK : ISOLASI DAN KARAKTRISASI ENZIM KITINASE Purkan, Purkan; Azizah, Badi?atul; Baktir, Afaf; Sumarsih, Sri
Molekul Vol 9, No 2 (2014)
Publisher : Universitas Jenderal Soedirman

Enzim kitinase banyak digunakan dalam bidang medis, makanan, bioteknologi dan lingkungan. Banyaknya kebutuhan enzim kitinase menuntut penyediannya yang murah dan melimpah dengan teknologi produksi yang sederhana. Penelitian ini bertujuan untuk isolasi mikroba kitinolitik dari cairan fermentasi sampah organik, produksi dan uji aktivitas enzim kitinase serta mengetahui karakteristik dari enzim kitinase. Isolasi mikroba telah dilakukan dengan metode spread plate. Aktivitas kitinase ditentukan secara kualitatif dengan pengukuran indeks kitinolitik dan secara kuantitatif dengan pengukuran absorbansi menggunakan spektrofotometer Uv-Vis pada panjang gelombang 660 nm berdasarkan banyaknya substrat kitin yang dihidrolisis oleh enzim kitinase. Satu dari beberapa isolat yang didapatkan, yaitu isolat A1 menunjukkan aktivitas kitinolitik tertinggi, yaitu sebesar 1,21. Hasil identifikasi mikrobiologi menunjukkan bahwa isolat A1dinyatakan sebagai Pseudomonas pseudomallei. Bakteri ini mampu menghasilkan kitinase secara optimum pada jam ke 18 waktu fermentasi, dengan penambahan molase 0,5% (b/v) dan 1% kitin (b/v) pada media produksinya. Kitinase yang dihasilkan P. pseudomallei menunjukkan aktivitas optimum pada suhu 50 °C danpH sebesar 6.

Biochemical Properties of Mercuric Reductase from Local Isolate of Bacillus sp for Bioremediation Agent Purkan, Purkan; Nuzulla, Yuliana Firdausi; Hadi, Sofijan; Prasetyawati, Endang Triwahyu
Molekul Vol 12, No 2 (2017)
Publisher : Universitas Jenderal Soedirman

Mercuric reductase is the important enzyme which catalyzes a reduction of a toxic Hg2+ to non-toxic Hg0. The enzyme which has been potentially used as mercury bioremediation agent is produced by mercury resistant bacteria. These research aims are to determinate the resistance level of a local Bacillus sp to HgCl2 in media, to determine the mercuric reductase activity from the bacteria, and to determine the biochemical properties of the mercuric reductase. The Bacillus sp was grown in the Nutrient Broth media with various of 0; 20; 40; 60; 120; and 160 µM HgCl2 to know the response of the bacteria against mercury, The cell growth of Bacillus sp was measured by optical density (OD) method of at ? 600 nm. The mercuric reductase activity was assayed in the solution of MRA (Mercury Reductase Assay), then the oxidized NADPH was observed by the spectrophotometry method at ?340 nm. The result showed that the Bacillus sp has been resistant to media containing mercury at 120 µM, but the microbial growth was decreased by 50% in media containing mercury 80 µM. The Bacillus sp could produce highly the mercuric reductase enzyme at 16 hours of growth time with enzyme activity as 0.574 Unit/µg. The mercuric reductase from the bacteria has an optimum activity at pH 6 and temperature 37 °C

PENCIRIAN PRODUKSI AMILASE OLEH SACCAROMYCES CEREVISIAE W303A REKOMBINAN Thontowi, Ahmad; Puspaningsih, Ni Nyoman Tri; Hadi, Sofjan; Purkan, Purkan; Ni'mahtuzahroh, Ni'mahtuzahroh; Irawan, Bambang
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i3.3464

ABSTRACTCharacterization of Amylase Production by Saccharomyces cerevisiae W303A Recombinants. Cloning of amylase gene from Endomycopsis fibuligera ITB.R.cc.64 into S. cerevisiae W303a can effectively increase the yeast function to digest starch directly into ethanol. Production of amylase by S. cerevisiae W303a recombinants (I and P) were done by growing in yeast peptone starch (YPS) medium. The result showed that the recombinants could be produced of amylase by gave clear zone after staining by iodium vapor. The optimum condition of production of amylase by S. cerevisiae W303a recombinants were pH 7.0, 40?C temperature incubation, and gave maximum activity after 36 hours incubation. Amylase activity of I was higher than P recombinant for these condition respectively.Key words: Characterization, amylase, S. cerevisiae W303a

SOSIALISASI MANFAAT DAN PEMBUATAN NATTO DAN SOY YOGURT MELALUI KEGIATAN WEBINAR DAN PRAKTEK Sumarsih, Sri; Baktir, Afaf; Khairunnisa, Fatiha; Harsini, Muji; Purwaningsih, Aning; Wafiroh, Siti; Hartati, Hartati; Purkan, Purkan; Hadi, Sofijan
Jurnal ABDI: Media Pengabdian Kepada Masyarakat Vol. 7 No. 1 (2021)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/ja.v7n1.p103-107

Natto and Soy Yogurt are fermented food products that are very beneficial for improving health. This community service activity aims to socialize the benefits and make them known to the wider community. However, community service activities during the Covid-19 pandemic cannot be carried out face-to-face and have activities with the community in large numbers. Therefore, this community service activity was carried out through webinar and practical activities. Online seminars was chosen so that the coverage was wider and more people could participate in the pandemic. Face-to-face and community activities are carried out with a limited number of participants, according to health protocols. The level of success, benefit and acceptance of the community from this community service activity is known from the responses of the participants during the activities. Based on the results of the assessment/ response of participants who were present virtually and those present at the location, In general it can be concluded that the PKM activity entitled "socializing the benefits and making natto and soy yogurt through webinars and practices" is going well, but better preparation is still needed.The material presented by the resource person is suitable for the current pandemic conditions, easy to understand, easy to practice and can be developed for home businesses. The Zoom Meeting application can be used as a medium for community service activities in pandemic conditions even though it still cannot reach the wider community. Â Keywords: Socialization, natto, soy yogurt, webinar, practical

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