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All Journal Jurnal Natur Indonesia Molekul: Jurnal Ilmiah Kimia
Zusfahair Zusfahair, Zusfahair
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry

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Isolasi, Pemurnian dan Karakterisasi Lipase Bakteri Hasil Skrining dari Tanah Tempat Pembuangan Akhir (TPA) Gunung Tugel Banyumas Zusfahair, Zusfahair; Setyaningtyas, Tien; Fatoni, Amin
Jurnal Natur Indonesia Vol 12, No 2 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (479.835 KB) | DOI: 10.31258/jnat.12.2.124-129

Abstract

A bacterial lipase producer was isolated from garbage dump soil and was identified its genus. Lipase was extractedaccording to production time optimized, purified using ammonium sulfate fractionation and gel chromatograph.Determination of enzyme characteristic studied were influence of pH, temperature, various metals to lipaseactivity. The result of this research shows that the genus of isolated bacteria which produced lipase wasAcinetobacter sp., the lipase optimum production time is about 18 hours with the activity is about 115 unit/mL. Thehighest activity of lipase fractionation using ammonium sulfate is about 45% and the highest activity of purifyingwith filtration gel chromatograph column using Sephadex G-150 at 24 th fraction. Lipase from crude extract andpurifying product at this fraction has optimum pH 6 and optimum temperature is about 40 oC. Lipase to be classifiedas metalloenzyme that shows with decreasing the activity after added the EDTA. Metals ion, such as Cu 2+ and Zn2+were inhibited the lipase activity. Ca 2+ ion could increase lipase crude extract activity but inhibited the activity oflipase purifying product. Hg2+ ion could increase the activity of lipase purifying product.
Isolasi dan Karakterisasi Protease Ekstraseluler dari Bakteri dalam Limbah Cair Tahu Fatoni, Amin; Zusfahair, Zusfahair; Lestari, Puji
Jurnal Natur Indonesia Vol 10, No 2 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (102.484 KB) | DOI: 10.31258/jnat.10.2.83-88

Abstract

Protease has been used in large application industrial process such as detergent, leather, textil, softdrink, andmedicine. In order to find unique protease, many substances were explored as proteases of bacteria sources. Inthis study, tofu liquid waste was used as a source of bacteria producing proteases. Waste sample was growth inskim milk agar medium showing proteases activity, it was used to produce extracellular protease. The microbialcolonies were identified as Staphyllococcus sp. Protease was extracted with 5000 g centrifugation at 4 0C, andpurificated with ammonium sulphate precipitation continued with dialisis. Optimum production time, pH, metal ion,EDTA, specific activity, KM, and Vmaks were studied for enzyme characterization. Volume of crude enzyme was 300ml, with spesific activity of 3.55 U/mg. Protease obtained from 60% ammonium sulphate fraction had the highestspecific activity of 68.22 U/mg. Study on the protease characterization revealed that optimum temperature of thisenzyme was 400C. The optimum pH of the enzyme was found to be 8.0. The kinetic parameters K M dan Vmaks withcasein as substrate were 0.31% and 51.55 U/ml. Some inhibitory effect was observed in the presence of EDTA, Cu +2,Co+2, Zn+2, and enzyme activity was stimulated by Mg+2, indicating that this ion had a functional role in the molecularstructure of the enzyme.
Hidrolisis Pati Ganyong (Canna edulis) dengan Amilase Bakteri Flavobacterium sp. PTBT I untuk Produksi Bioetanol Ningsih, Dian Riana; Zusfahair, Zusfahair; Fatoni, Amin
Jurnal Natur Indonesia Vol 15, No 2 (2013)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.385 KB) | DOI: 10.31258/jnat.15.2.92-98

Abstract

Bioethanol is an alternative energy of fuels produced from vegetable materials. Vegetable materials that can be used as rawmaterial for bioethanol is ganyong because it contains 22.60 g starch in 100 g ganyong. The production of bioethanol fromstarch material consisted of two steps, hydrolysis and fermentation. One of the steps to increase the value of bioethanolfrom starch of ganyong was hydrolysis process using thermostable amylase enzyme isolated from Flavoacterium sp.PTBT I bacteria was isolated from hot spring of Pancuran Tujuh Baturraden. The aim of this research was to use thermostableamylase to hydrolyze starch of ganyong and glucose produced to result bioethanol. The result of this research showed thatthe optimum condition hydrolysis starch of ganyong was using thermostable amylase acquired at substrate concentrationof 3% (b/v), and incubation time of about 75 minutes. The value of bioethanol increased with time of fermentation, from thefirst to fourth day, which was 0.8361; 2.2379; 5.7590 and 10.5787% (v/v), respectively.
Determination of Cu and Pb concentrations based on urease activity inhibition of Durio zibethinus L. seeds Zusfahair, Zusfahair; Fatoni, Amin; Ningsih, Dian Riana; Riapanitra, Anung
Molekul Vol 16, No 2 (2021)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (217.71 KB) | DOI: 10.20884/1.jm.2021.16.2.736

Abstract

The determination of heavy metal concentrations has been carried out using sophisticated instruments, and therefore a simple and reliable alternative method is needed as a comparison. The study aimed to determine Cu and Pb concentration of standard solution using the urease activity inhibition method of Durio zibethinus L.  seeds.  The research started with urease extraction from Durio D. zibethinus L. seeds. The activity of the obtained extract was determined using the Nessler method. The optimum substrate concentration was also determined. Urease activity inhibition was carried out using various metal solution concentrations, which continued by plotting a log graph of urea concentration vs. %inhibition. The obtained graph would then determine the metal concentration in a synthetic water sample. The data was then compared to the measurement, determined by the Atomic Absorption Spectrophotometry (AAS) method. Results of the study showed that the urease activity of D. zibethinus L.seeds was 296.774 U/mL. Urease activity was optimum at a urea concentration of 0.3 M. The comparison Cu, and Pb concentration determination using the urease inhibitory activity and AAS methods showed no significant difference at 95% confidence level. This research showed that urease of D. zibethinus L. seed could be used to determine Cu and Pb's concentration based on its inhibiting activity.
Co-Authors A.A. Ketut Agung Cahyawan W AMIN FATONI Anung Riapanitra Dian Riana Ningsih Tien Setyaningtyas