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All Journal Padjadjaran Journal of Dentistry

Roosje Rosita Oewen, Roosje Rosita

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Author-ID : 597195

Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Articles

Effect of short one-third lower face toward mastication performance in Deutero Malay Anggraeni, Dian; Oewen, Roosje Rosita; Runkat, Jakobus; Hidayat, Syarief
Padjadjaran Journal of Dentistry Vol 26, No 3 (2014): November
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

The one-third lower face height belongs to ideal proportion, ratio 1:1:1, of vertical face height. The one-third lower face height could be influenced by mastication. The one-third lower face height that shorter than normal ratio is generally followed by deep bite which may lead to temporomandibular disfunction even so eustachian tube disfunction, this condition may interfere mastication function, which can be measured by mastication performance. The research objective was to determine the effects of short lower face height to the mastication performance based on overbite. The research method was a cross-sectional study with epidemiology survey type. Research subjects 12-15 years old junior high school with Deutero Melayu Sub races in Bandung. Sampling technique used multistage random sampling by determining the size of the sample based on potluck sample and gained 24 children (group 1) with the normal ratio of vertical face height and 27 children with short lower facial height which were 11 children overbite normal (group 2) and 16 children deep bite (group 3). Mastication performance by 20 times chewing artificial test food then followed by sieving test. Mastication performance value represented by the median particle size (MPS) and the distribution of the particle distribution (b). The results of average MPS and b between group 1, 2 and 3 used statistical analysis MPS with ANOVA test which Fhit=5.56 and pvalue=0.0075, b which Fhit=3.41 and pvalue=0.0430 showed significant differences. Continued with group T test MPS (value = 0.0925) dan b (value = 0.2076) between group 1 and 2 showed a non significant differences. While between group 1 and 3 showed a highly significant differences in MPS (value = 0.0037 and ï¡ = 0,01) and a significant differences in b (value = 0.0141 and ï¡ = 0,05). Conclusions of this study were children with short lower face height and normal overbite did not decrease the mastication performance while children with deep bite decrease the mastication performance.

The effects of ethyl acetate fraction of Ananas Comosus (L.) Merr. of tongue cancer cell growth inhibition Supriâs Clone-1, invitro Martina, Maureen; Oewen, Roosje Rosita; Riyanti, Eriska; Syawqie, Achmad; Supriatno, S.
Padjadjaran Journal of Dentistry Vol 23, No 2 (2011): July
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Ananas comosus (L.) Merr has several purposes which include antioxidant and anti-inflammatory activity that shows some pharmacological effects and the subject of anti-cancer or anti-cancer supporting material. The research objective was to analyze the effects of ethyl acetate fraction of Ananas comosus (L.) Merr. of tongue cancer cell growth inhibition Supriâs clone-1 (SP-C1). This type of study was a research laboratory. Next, cell growth inhibition testing by the ethyl acetate fraction of Ananas comosus (L.) Merr. with various concentrations (0; 62.5; 125; 250; 250; 500 and 1000 microgram/ml) using the MTT assay test. Growth barriers identified by Biorad microplate tool reader with a wavelength of 540 nm. The number of SP-C1 cells examined was 2 x 104 cells/wells with incubation time 24 and 48 hours. Data were analyzed using a two-ways ANOVA followed by post hoc test (LSD test) with 95% significance level. The results showed ethyl acetate fraction of Ananas comosus (L.) Merr. able to inhibit the growth of cancer cells SP-C1. Various concentrations of ethyl acetate fraction of pineapple were highly significant, meaning that the concentration effect on cell growth of SP-C1. Similarly, incubation time effect on the growth of SP-C1 cells that were very meaningful. The biggest obstacle effect of ethyl acetate fraction of Ananas comosus (L.) Merr. occurred at a concentration of 1000 ug/ml (43.45%) with an incubation time of 48 hours. Conclusion of this study was the fraction of ethyl acetate Ananas comosus (L.) Merr. has the effect of inhibiting the growth of cancer cells SP-C1.

The effects of temporo mandibular joint disorder with clicking symptom on children mastication performance in Deutero Malay race children aged 12-15 years Hartman, Henri; Pertiwi, Arlette Suzy Puspa; Runkat, Jakobus; Herdiyati, Yetty; Oewen, Roosje Rosita
Padjadjaran Journal of Dentistry Vol 26, No 3 (2014): November
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Temporo Mandibular Joint Disorder (TMD) could be caused by forward head posture. Articular sound/TMJ clicking is the most often sign and symptom for TMD that could happen in human being. The presence of TMD such as TMJ clicking would cause an imbalance masticatory system. The purpose of this research is to investigate TMJ clicking effects to masticatory performance. This research was cross-sectional study with a type of epidemiology survey. Subject were children aged 12-15 years old Deutero-Malay sub-races Live in Bandung and was taken using multi-stage random sampling technique. Subject; consisted of 24 children as control group and 28 children as TMJ clicking group. Both group were then checked for masticatory performance using multiple sieve method and 20x chewing of artificial test food. Mastication performance value represented by median particle size (MPS) particle distribution (b) for each group. MPS from TMJ clicking group (3.0571,SD=0.9990) showed higher value than control group (2.28958,SD=0.66838). Statistic analysis with t-test showed that thereâs a significant result in both of group (pvalue=0,0024, Î± = 0,05). Conclussion, temporo mandibular joint clicking subject has lower masticatory performance.

Ethanol extract of mangosteen (Garcinia Mangostana Linn) peel effect in inhibiting the growth of human tongue cancer cells Supriâs Clone 1, invitro Suanto, Edi; Oewen, Roosje Rosita; Sasmita, Inne Suherna; Supriatno, S.; Supratman, Unang
Padjadjaran Journal of Dentistry Vol 23, No 2 (2011): July
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

The incidence of tongue cancer in Indonesia reached 1.01% of all cancers and 42% of oral cavity cancer. Tongue cancer therapies including chemotherapy, radiotherapy, surgery, and all three combined therapy. Search for anti-cancer drugs currently switched on herbal plants, one of which is the mangosteen. Has the properties of mangosteen peel extract inhibited the growth of cancer cells. The purpose of the study, obtain IC50 of ethanol extract of mangosteen peel in inhibiting the growth of human tongue cancer cells SP-C1. Research carried out on 96 preparations of human tongue cancer SP-C1 were incubated with ethanol extract of mangosteen peel, preparations were classified in two groups of incubation time (24 hours and 48 hours) and each group will be given preferential treatment over 6 randomly different concentrations: 0 (control), 62.5 Î¼g/mL, 125 Î¼g/mL, 250 Î¼g/mL, 500 Î¼g/mL and 1000 Î¼g/mL. Model experiments were 2 x 6 factorial experiment with eight replication for each cell. Test results with ANAVA, incubation (24 and 48 hour) SP-tongue cancer cells with various concentrations of C1 ethanol extract of mangosteen peel gives a highly significant, indicating differences cancer cell growth inhibition. Incubation time factor showed the long incubation effect on cancer cell growth inhibition. Furthermore, by Newman Keuls test, showed 500Î¼g/mL concentrations of 24-hour incubation had the best effect. Conclusion of the study of ethanol extract of mangosteen peel could achieve with IC50 values of cell growth resistance 50.3% at a concentration of 500 Î¼g/mL and an incubation time of 24 hours.

The effect of ethanol extract of mangostene (Garcinia Mangostana Linn.) peel on tongue cancer cells Supris Clone-1 apoptosis, in vitro Satari, Mieke Hemiawati; Titiana, Maria Aurora; Oewen, Roosje Rosita
Padjadjaran Journal of Dentistry Vol 23, No 3 (2011): November
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Apoptosis or programmed cell death serves to regulate physiological and pathological conditions. Garcinia mangostana Linn. is one of the medical herbs that is widely used to suppress human tongue cancer growth by inducing apoptosis. The research aimed to analyze the effect of ethanol extract of Garcinia mangostana Linn. on tongue cancer cells apoptosis Supris Clone-1 (SP-C1). The type of the research was an experimental laboratory. Tongue cancer cells SP-C1 were treated by various ethanol extract concentrations (0, 300, 400, 500, 600, 700 pg/ml) of Garcinia mangostana Linn. to detect the apoptosis, which was done by acridine orange and ethidium bromide coloring tests. The number of tongue cancer cells SP-C1 2x104 cells/dish. Observation on cells apoptosis was observed by a fluorescent microscope with 40 x magnification. The data were analyzed using one-way ANOVA and was followed by Post Hoc test (Tukey-test) with 95% significance level. The result showed that tongue cancer cells SP-C1 apoptosis treated by various ethanol extracts of Garcinia mangostana Linn. increase significantly. The highest effect of apoptosis was detected at the 700 pg/ml concentration that has an effect on the percentage of tongue cancer cells apoptosis by 65%.

Anti-tumor agent celecoxib activity towards SP-C1 tongue cancer cells invasion (in vitro) Achmad, Harun; Satari, Mieke Hemiawati; Oewen, Roosje Rosita; Supriatno, S.
Padjadjaran Journal of Dentistry Vol 23, No 1 (2011): March
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Invasion is a characteristic of the occurrence of cancer and indicates the cancer cells capability to destroy and degrade the border between the epithet and basal membrane to further spread into the surrounding extra-cellular matrix. The purpose of this research was to find the existence of impediment at the SP-C1 tongue cancer cell using celecoxib chemopreventive medication. The SP-C1 tongue cancer cells were treated in vitro using celecoxib medication as a research subject at the following concentrations 5, 10, 25, 50, 75, 100, 125%; and 0 as control group (only DMEM growth medium treatment). Pure experimental testing was carried out for 24 and 48 hours, with observation and calculation of an average number of SP-C1 tongue cancer cells. The data collected were analyzed using the ANOVA test with Newman Keuls paired range test or t-test. Research results indicated that the average number of SP-C1 tongue cancer cells invasion after administration of celecoxib medication based on administration concentration and time statistically yielded significant results. The ANOVA test results were statistically significant, that is, average occurrence of the number of SP-C1 tongue cancer cells due to the use of celecoxib at certain concentrations compared to that without celecoxib was different. At celecoxib of zero (control) concentration was 24.4 with celecoxib concentration starting at 5 up to 125% experienced a decline from its average 11 to become 2.3. The conclusion of the research was that the greater the celecoxib concentration administered, the greater the effect on the impediment of SP-C1 tongue cancer cell invasion.

Differences in dental age of small gestational age children based on the severity of enamel dental defects Kania, Annisa Maya; Oewen, Roosje Rosita; Suwondo, Williyanti
Padjadjaran Journal of Dentistry Vol 22, No 2 (2010): July
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Small Gestational Age (SGA) is the term used for a new born baby with birth-severe below the 10th percentile on the intrauterine Lubchenco curve, due to Intra Uterine Growth Restriction (IUGR) which can cause defects of several organs such as deciduous teeth enamel. Dental age is the indicator for growth and development of the teeth that determine biological age which is more accurate than chronological age and could be determined by calcification or teeth eruption. The purpose of this study was to obtain dental age in SGA children based on the enamel defect severity level of deciduous teeth as compared to the chronological age. This study was a comparative analytic study with given sample size, conducted towards 18 SGA children aged 4-6 years old, and 21 children with Appropriate Gestational Age (AGA) aged 4-6 years old as the control group. Deciduous teeth enamel defect severity level determined by the developmental defect of enamel (DDE) score FDI modified. In order to determine the permanent teeth growth and development, the indicator was based on dental age using the panoramic radiograph with Dermijian method. The t-test was used to compare the permanent teeth dental age difference between SGA and AGA children as well as the permanent teeth dental age difference of SGA children based on the enamel defect severity level of deciduous teeth as seen in their chronological age. The result showed that there was a difference in the permanent teeth dental age between the SGA and AGA children at chronological ages of 4, ,5, and 6 years old (t count = 3,36; 2,35; and 3,49). Based on the average of permanent teeth dental age, the value of AGA children was higher than SGA children. Furthermore, it also showed that the severe score of EDS in the SGA children at the age of 4, 5, and 6 years old, their permanent teeth dental age was lower than the mild EDS (t count = 1,45). The conclusion of this study was the permanent teeth dental age in SGA children was lower than the AGA children as well as difference between the permanent teeth dental age in SGA children based on their deciduous teeth enamel defect severity, and the severe defect refered to slower permanent teeth dental age than the mild defect.

Co-Authors Achmad Syawqie Anak Agung Gede Sugianthara Arlette Suzy Puspa Pertiwi Edi Suanto, Edi Eriska Riyanti Harun Achmad, Harun Henri Hartman, Henri Inne Suherna Sasmita Jakobus Runkat, Jakobus Kania, Annisa Maya Maria Aurora Titiana, Maria Aurora Maureen Martina, Maureen Mieke Hemiawati Satari S. Supriatno, S. Suwondo, Williyanti Syarief Hidayat Unang Supratman Yetty Herdiyati, Yetty

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