Masfufatun ., Masfufatun
Departemen Biokimia, Fakultas Kedokteran, Universitas Wijaya Kusuma Surabaya

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The Potential of Candida Biofilm Protein as Bioreceptor for Candidiasis Immunoassay Masfufatun Masfufatun; Loo Haryanto; Harsono Harsono
Berkala Kedokteran Vol 14, No 1 (2018)
Publisher : Fakultas Kedokteran Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (293.058 KB) | DOI: 10.20527/jbk.v14i1.4539

Abstract

Abstract: Candidiasis or infection that is caused by Candida has become a new list of the therapeutical problems recently. The difficulties in diagnosing are the main cause of the unsatisfactory results from common therapies and diagnosis methods. This has urged researchers to find alternative ways in candidiasis diagnosis such as serology-based detection using antigen or antibody development. The aim of this study was to evaluate the potential of protein derived from Candida albicans biofilm as bioreceptor on candidiasis immunoassay through Dot Blot method. The research method used descriptive method with the following stages: (1) preparation of Candida albicans biofilm (2) extraction of Candida albicans protein through enzymatic and mechanical methods, (3) determination of protein molecular weight with SDS-PAGE (4) production of polyclonal anti- candida and (5) analysis of protein extract as bioreeceptor on dot blot. Profile of biofilm proteins on SDS-PAGE analysis were shown on molecular weight 27,42; 29,89; 38,10; 44,90; 48,75; 52,92; 55,14; 59,86; 70,56; 87,36; 102,54;115,05; 130,14;143,14;181,53 kD. There were differences in the intensity of dots in the control group (44070) and treatment groups (63170.5). It is noticeable that biofilm protein extract of C. albicans can be used for induction of anti-Candida polyclonal antibody production as the potential candidate of bioreceptor in candidiasis immunoassay. Keywords: SDS-PAGE, polyclonal antibody, immunoassay, dot blot, biofilm
The Potency of Protein Extracts from Candida albicansas Bioreceptor on Immunosensor for Diagnosis of Candidiasis ., Masfufatun; Kumala, Noer; Baktir, Afaf
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Abstract

Currently diagnosis of candidiasis still usingthe traditional standard blood culture method. The traditional method were less sensitive andtime consuming. The purpose of this research were to develope the more sensitive immunosensor based method, and to examine the potency of C. albicans protein extract as bioreceptor to  detect  C. albicans and its biofilm in the blood of candidiasis patients.The research methods include: (1) preparation of  digestive gland liquid of snail (Achatina fulica); (2) extraction of protein from C. albicans  through  enzimatis and mechanic methodsand (3) analyzing the protein extract as bioreseptor through immunodot assay.The research results showed that the snail enzymes has protein content 1.35 mg/ml and  specific activity 1.96 unit/mg. The snail enzyme hydrolyzed  the cell wall of C. albicans  with and without sonication, producedplanktonic extracell protein extract (PEP) and biofilmextracell proteinextract (PEB), planktonic intracell protein extract (PIP), and biofilmintracell protein extract (PIB), withprotein content 1.44; 1.29; 1.29 and 1.21 mg/ml respectively. Thebiofilm intracell protein (BIP) showed antigenic property towardantibody anti-Candida (positive control),giving red spot on imunodot assay. Immunodot assay can distinguishnegative control serum (health man) and positive Candidiasis control by using antigen 1 mg/ml and50ml serum. Keywords: C. albicans, candidiasis, biofilm, immunodot assay
Kadar IL-6 dan IL-10 Serum pada Tahapan Inflamasi di Rattus norvegicus yang terinfeksi Candida albicans Masfufatun, Masfufatun; Tania, Putu Oky Ari; Raharjo, Loo Hariyanto; Baktir, Afaf
Jurnal Kedokteran Brawijaya Vol 30, No. 1 (2018)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2018.030.01.4

Abstract

Insiden kandidiasis sangat tinggi di dunia yang disebabkan Candida albicans. Agen antifungi telah banyak dikembangkan, namun resistensi terhadap antifungi masih menjadi suatu permasalahan. Resistensi antifungi ini diakibatkan oleh kemampuan C. albicans dalam membentuk biofilm. Terbentuknya biofilm dari infeksi C. albicans diawali oleh proses inflamasi. Proses inflamasi oleh Candida albicans terjadi pada beberapa tahap yang salah satu indikatornya adalah dilepaskannya sitokin proinflamasi (IL-6) dan anti inflamasi (IL-10). Penelitian ini bertujuan untuk mengetahui kadar IL-6 dan IL-10 pada tiap tahapan inflamasi C. albicans pada intestinal pada tikus putih. Penelitian ini menggunakan 32 ekor tikus wistar (Rattus norvegicus) yang dibagi menjadi 2 kelompok, yaitu kelompok kontrol dan perlakuan. Kelompok perlakuan diinokulasi dengan C. albicans. Variabel yang diukur adalah kadar IL-6 dan IL-10 pada hari ke-7, 14, 21, 28 dan 35 setelah inokulasi C. albicans. pengambilan data sebanyak 5 kali dilakukan untuk mewakili tahapan pembentukan biofilm candida. Respon imun pada setiap tahap pembentukan biofilm ditunjukkan dengan kadar sitokin IL-6 dan IL-10 serum darah dengan metode ELISA. Hasil penelitian menunjukkan bahwa kadar IL-6 dan IL-10 pada hari ke-7 dan 14 sebesar 0pg/mL. Pada hari ke 28 telah terjadi proses inflamasi akut  pelepasan IL-6 dan IL-10 dengan kadar tertinggi masing-masing 31,75±9,99pg/mLdan 757,94±576,73pg/mL. Selanjutnya pada hari ke-35, kadar sitokin IL-6 dan IL-10 masing-masing mulai menurun menjadi 28±11,53pg/mL dan 349,5±188,48pg/mL. Respon imun tubuh mulai terjadi pada tahap awal pembentukan biofilm C. albicans intestinal tikus, sehingga kedepannya dapat dikembangkan terapi imunomodulator terhadap kandidiasis sehingga dapat menghambat pembentukan biofilm C. albicans.
THE POTENCY OF Candida albicans PROTEIN EXTRACT AS BIORESEPTOR ON IMMUNOCENCOR TO DIAGNOSE CANDIDIASIS Masfufatun, Masfufatun; Sudibya, Akhmad; Kumala, Nur
Jurnal Ilmiah Kedokteran Wijaya Kusuma Vol 3, No 2 (2014): edisi Oktober 2014
Publisher : Universitas Wijaya Kusuma Surabaya

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Abstract

So far, diagnosing on candidiasis has still been limited in standard blood culture. The traditional method of this microbiology culture is less sensitive, many patients with candidasis infection have negative blood culture, and need a long time. This case encourage many researchers to use another alternative methode to diagnose. The purpose of this research is to examine the potency of Candida albicans protein extract as bioreseptor on immunocencor to  detect  Candida albicans and its biofilm in the blood of candidiasis patients. The research method which is used is descriptive with the following steps : (1) isolating enzim from the liquid of digestive gland of snail (Achatina fulica); (2) extraction of protein candida albicans  through  enzimatis and mechanic methods and (3) Analyzing the protein extract as bioreseptor through immunodot assay.The research result shows that the snail enzyme has the protein content 1.35 mg/ml and the specific activity 1.96 unit/mg. This snail enzyme can hydrolyze  the cell wall of Candidia albicans  and, with the help oh sonication, produce extract of planktonic extracel protein (PEP) and biofilm (PEB), extract of planktonic intracell protein (PIP), and biofilm (PIB), with each protein content 1.44; 1.29; 1.29 and 1.21 mg/ml. The characeristic of biofilm intracell protein (PIB) is antigenic on antibody anti-candida (positive control) with giving red spot on immunodot assay. Immunodot assay can distinguish negative control serum (health man) and positive Candidiasis control by using antigen 1 ng/nl and 50nl serum.
ISOLASI DAN KARAKTERISASI ENZIM SELULASE masfufatun, masfufatun
Jurnal Ilmiah Kedokteran Wijaya Kusuma 2009: edisi khusus Desember 2009
Publisher : Universitas Wijaya Kusuma Surabaya

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Abstract

Carboxy Methyl Cellulose (CMC) is a derivative of cellulose soluble in water. Therefore, CMC easily hydrolyzed into simple sugars by the enzyme cellulase. Snails are animals soft, easy to breed and utilize cellulose as a source of energy and protein content is high enough. Therefore, snails can be used as a source of cellulase enzyme to hydrolyze carboxy Methyl Cellulose (CMC) This research aims to isolate cellulase enzyme from the snail, Achatina fulica and determine itscharacterization. Glucose levels produced from cellulose enzyme activity were analyzed by using the method Semogy-Nelson. From this research it turns out cellulase enzyme isolated from hepatopankreas snail, Achatina fulica has a specific activity of 2.85 U / mg protein and activity 50oCdan temperature optimum at pH 5.16 and have had kinetic parameters Vm price of 0.23 mg / mLper minute and Km is 0.53 mg / mL. Part enzyme cellulose getting fed at a concentration of 4%.
PENGARUH SUHU DAN WAKTU PENYIMPANAN TERHADAP VITAMIN C DALAM JAMBU BIJI (Psidium Guajava) masfufatun, masfufatun; Widaningsih, Widaningsih; Indahsari, Nur Kumala; Rahayuningsih, Tri
Jurnal Ilmiah Kedokteran Wijaya Kusuma Vol 2, No 1 (2010): Edisi Januari 2010
Publisher : Universitas Wijaya Kusuma Surabaya

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Abstract

The purpose of this study was to determine whether there is influence of temperature and storage time on vitamin C content in guava. This study is an experimental research menggunkan Completely Randomized Design (CRD) with two factors and two repetitions. Of the two factors were obtained eight combinations of treatments. Each - each treatment was repeated twice, in order to obtain 16 replicates. The parameters used were vitamin C content in guava. Guava that has been stored in a specific time and temperature peel and blend until homogeneous to obtain slurry. Furthermore slurry disentrifuge guava and filtered. Filtrate obtained its vitamin C content determined by titration method Iodometri. Data obtained with ANOVA dinalisis. If there is any real difference followed by Least Significant difference test (LSD). Results showed that storage time and temperature affect the levels of vitamin C in guava fruit ripe. The longer the storage time and the higher the temperature the lower the vitamin C content. Vitamin C content in guava stored at room temperature for 10 days decreased by 46.35% and the cold temperature is only 39%.
POTENTIAL EXTRACT OF Moringa Oleifera AS HEPATOPROTECTIVE IN WHITE RATS (Rattus novergicus) INDUCED TOXIC DOSES OF PARACETAMOL Indahsari, Noer Kumala; masfufatun, masfufatun; D.R, Emilia Devi
Jurnal Ilmiah Kedokteran Wijaya Kusuma Vol 5, No 1 (2016): Edisi Maret 2016
Publisher : Universitas Wijaya Kusuma Surabaya

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Abstract

Moringa Oleifera is a plant that contains chemical compounds that are useful, such as flavonoids. The ability of this flavonoid compound that can capture free radicals cause damage and hepatoprotektan hepar. Purpose of study was to determined levels of Moringa leaf extract which can overcome the effects of liver damage caused by toxic doses of paracetamol through MDA, SGOT and SGPT Method used in this laboratory experimental study is a Randomized Post Test Only Control Group Design with the following stages: 1. Moringa Leaf Extraction with Ethanol 96%; Try 2.Preparasi animals, 3. Treatment of Animals Try the extract of leaves of Moringa 3 dose is: 250mg / 200BB rat (dose of A), 500mg / 200BB mice (dose B), 1000mg / 200BB mice (dose C) for 14 days in combination with paracetamol 2 g / 200BB mice, compared to the negative control group (group given just paracetamol 2 g / 200BB rat) and the positive control group (the group who were given regular feed) for 14 days.Results : turned out to be no difference in the reduction in SGOT levels are statistically significant between the negative control group with high-dose treatment group ie the dose C with =0,016 smaller than 0.05, whereas a decrease in ALT levels were significantly decreased in the treatment group high dose is the dose C with =0,009 smaller than 0.05. While MDA group treated with the negative control group experienced an overall decline for the dose A with =0,05, dose B with =0,0011 and dose C with =0,001. Conclusion of this study showed that the extract of Moringa leaves can be potentially as an antioxidant in all doses at once can be as hepatoprotektor at high doses is 1000mg / 200BB Rattus Novergicus.
The Potential of Candida Biofilm Protein as Bioreceptor for Candidiasis Immunoassay Masfufatun, Masfufatun; Haryanto, Loo; Harsono, Harsono
Berkala Kedokteran Vol 14, No 1 (2018)
Publisher : Fakultas Kedokteran Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jbk.v14i1.4539

Abstract

Abstract: Candidiasis or infection that is caused by Candida has become a new list of the therapeutical problems recently. The difficulties in diagnosing are the main cause of the unsatisfactory results from common therapies and diagnosis methods. This has urged researchers to find alternative ways in candidiasis diagnosis such as serology-based detection using antigen or antibody development. The aim of this study was to evaluate the potential of protein derived from Candida albicans biofilm as bioreceptor on candidiasis immunoassay through Dot Blot method. The research method used descriptive method with the following stages: (1) preparation of Candida albicans biofilm (2) extraction of Candida albicans protein through enzymatic and mechanical methods, (3) determination of protein molecular weight with SDS-PAGE (4) production of polyclonal anti- candida and (5) analysis of protein extract as bioreeceptor on dot blot. Profile of biofilm proteins on SDS-PAGE analysis were shown on molecular weight 27,42; 29,89; 38,10; 44,90; 48,75; 52,92; 55,14; 59,86; 70,56; 87,36; 102,54;115,05; 130,14;143,14;181,53 kD. There were differences in the intensity of dots in the control group (44070) and treatment groups (63170.5). It is noticeable that biofilm protein extract of C. albicans can be used for induction of anti-Candida polyclonal antibody production as the potential candidate of bioreceptor in candidiasis immunoassay. Keywords: SDS-PAGE, polyclonal antibody, immunoassay, dot blot, biofilm
THE POTENCY OF PROTEIN EXTRACTS FROMCandida albicans AS BIORECEPTOR ON IMMUNOSENSOR FOR DIAGNOSIS OF CANDIDIASIS Masfufatun -; Noer Kumala; Afaf Baktir
PROSIDING SEMINAR KIMIA PROCEEDING OF INTERNATIONAL CONFERENCE 2015
Publisher : PROSIDING SEMINAR KIMIA

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Abstract

Currently diagnosis of candidiasis still usingthe traditional standard blood culture method. The traditional method were less sensitive andtime consuming. The purpose of this research were to develope the more sensitive immunosensor based method, and to examine the potency of C. albicans protein extract as bioreceptor to detect C. albicans and its biofilm in the blood of candidiasis patients.The research methods include: (1) preparation of digestive gland liquid of snail (Achatina fulica); (2) extraction of protein from C. albicans through enzimatis and mechanic methods and (3) analyzing the protein extract as bioreseptor through immunodot assay.The research results showed that the snail enzymes has protein content 1.35 mg/ml and specific activity 1.96 unit/mg. The snail enzyme hydrolyzed the cell wall of C. albicans with and without sonication, produced planktonic extracell protein extract (PEP) and biofilm extracell proteinextract (BEP), planktonic intracell protein extract (PIP), and biofilm intracell protein extract (BIP), with protein content 1.44; 1.29; 1.29 and 1.21 mg/ml respectively. The biofilm intracell protein (BIP) showed antigenic property toward antibody anti-Candida (positive control),giving red spot on imunodot assay. Immunodot assay can distinguish negative control serum (health man) and positive Candidiasis control by using antigen 1 g/l and 50 l serum.
The Potency of Protein Extracts from Candida albicansas Bioreceptor on Immunosensor for Diagnosis of Candidiasis Masfufatun .; Noer Kumala; Afaf Baktir
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

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Abstract

Currently diagnosis of candidiasis still usingthe traditional standard blood culture method. The traditional method were less sensitive andtime consuming. The purpose of this research were to develope the more sensitive immunosensor based method, and to examine the potency of C. albicans protein extract as bioreceptor to  detect  C. albicans and its biofilm in the blood of candidiasis patients.The research methods include: (1) preparation of  digestive gland liquid of snail (Achatina fulica); (2) extraction of protein from C. albicans  through  enzimatis and mechanic methodsand (3) analyzing the protein extract as bioreseptor through immunodot assay.The research results showed that the snail enzymes has protein content 1.35 mg/ml and  specific activity 1.96 unit/mg. The snail enzyme hydrolyzed  the cell wall of C. albicans  with and without sonication, producedplanktonic extracell protein extract (PEP) and biofilmextracell proteinextract (PEB), planktonic intracell protein extract (PIP), and biofilmintracell protein extract (PIB), withprotein content 1.44; 1.29; 1.29 and 1.21 mg/ml respectively. Thebiofilm intracell protein (BIP) showed antigenic property towardantibody anti-Candida (positive control),giving red spot on imunodot assay. Immunodot assay can distinguishnegative control serum (health man) and positive Candidiasis control by using antigen 1 mg/ml and50ml serum. Keywords: C. albicans, candidiasis, biofilm, immunodot assay