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Yati Supriatin, Yati
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Chemistry Other

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Modification Of Carry-Blair Transport Media For Storage Salmonella typhi Supriatin, Yati; Rahayyu, Muqni
Jurnal Teknologi Laboratorium Vol 5 No 2 (2016): 2016 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (386.493 KB)

Abstract

The aim of this study was to determine transport media modification as alternative media to replace Carry Blair. One type of transport media that often use to carry faeces specimens suspected to contain Salmonella typhi is Carry-Blair media. Studies have been conducted experimentally by storing Salmonella typhi on alternative transport media with Peptone composition, disodium Phosphate, Sodium chloride, Calcium chloride, which is made using a semi-solid and Carry-Blair as a control. Three variety of storage was done (0 hour,6 hours,9 hours) at a temperature 4⁰-8⁰C and then Salmonella typhi was inoculated in Salmonella Shigella Agar using spread plate technique incubated during 24 hours at 37⁰C, counted the number of colonies by the plate count method using the colony counter. The results of ANOVA could be concluded that modification media could be use as alternative media replace Carry-Blair at 6 hours. Based on regression correlation test was assumed that the Salmonella typhi bacteria still life at less than 11 hours 54 minutes.
Efektivitas Variasi Garam Salmiak (NH4Cl) Dan Sentrifugasi Pada Pemeriksaan Basil Tahan Asam Penderita Tuberculosis Siregar, Saadah; Supriatin, Yati; Noor, Lanny
Jurnal Teknologi Laboratorium Vol 6 No 2 (2017): 2017 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (524.857 KB) | DOI: 10.29238/teknolabjournal.v6i2.91

Abstract

According to a report by WHO Indonesia, it is a case number three infected with tuberculosis. Tuberculosis disease is caused by Mycobacterium tuberculosis that belongs to acid-fast bacilli bacteria (BTA). Sputum smear examination microscopically still has many deficiencies caused by mucoprotein in sputum so that BTA cannot be separated from sputum, this is causing a false negative in reading result Microscope. The objective of this research is to know the most effective variation of concentration, speed and time of centrifugation on smear examination in tuberculosis patients. The research method used is experimental method and Design of complete Random Block design. Data were analyzed by statistic test with ANOVA test on SPSS. The result of the research is that at concentration 2% NH4Cl It can increase the number of BTA with optimal centrifugation speed / effective at 5000 rpm for 15 minutes. Suggestion for further research is to use salmaniac salt concentration with a concentration lower than 2%.
Co-Authors Lanny Noor, Lanny Muqni Rahayyu, Muqni Saadah Siregar, Saadah