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Nur Amaliawati, Nur
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Chemistry Other

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Efektivitas Berbagai Konsentrasi Infusa Daun Salam (Eugenia polyantha Wight) terhadap Daya Antibakteri Staphylococcus aureus Secara In Vitro Apriani, Dewi; Amaliawati, Nur; Kurniati, Eni
Jurnal Teknologi Laboratorium Vol 3 No 1 (2014): 2014 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

Staphylococcus aureus is one of the acne-causing bacterias. Chemical compound in the Bay leaves are well-known as an antibacterial agent to inhibit the growth of Staphylococcus aureus. This study aimed to determine the influence of various Bay leaf (Eugenia polyantha Wight) infusing concentration toward the inhibition of Staphylococcus aureus growth. This research is experimental study with post-test and control group design. The sensitivity test methods toward Staphylococcus aureus were divided into five groups of bay leaf infusing concentration of 60%, 70%, 80%, 90%, and 100%. This method are used in 6 times to obtained 30 datas. Then, the datas were analyzed descriptively and analytically by ANOVA using SPSS 16.0 for Windows. The infusion of Bay leaves had inhibition activity against Staphylococcus aureus growth based on ANOVA test which showed a significance value 0.000 <0.05. There was a proof that the infusion of Bay leave have an effect to inhibit the growth of Staphylococcus aureus.
Pengaruh Lama Penyimpanan Makanan Khas Dayak Telu Ikan Furud (Garra sp) Terhadap Angka Lempeng Total (ALT) Putro, Fx Saptono; Amaliawati, Nur; Sherly, Sherly
Jurnal Teknologi Laboratorium Vol 5 No 1 (2016): 2016 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Telu merupakan makanan tradisional yang dibuat dari ikan furud maupun ikan tawar lainnya, dan dikomsumsi oleh masyarakat suku Dayak Lundayeh di daerah Kabupaten Malinau. Proses pembuatan diawali dengan proses pembersihan ikan, pemberian garam dan beras atau singkong rebus, kemudian didiamkan selama kurang lebih satu minggu. Penelitian ini bertujuan mengetahui jumlah bakteri Telu sebagai makanan tradisional Suku Dayak Lundayeh hasil fermentasi ikan Furud (Garra sp), setelah fermentasi 7 hari Ikan Telu Furud difermentasikan lagi selama 1, 2, dan 3 minggu. Terjadinya fermentasi ini dapat menyebabkan perubahan sifat bahan pangan, sebagai akibat dari pemecahan kandungan bahan pangan tersebut. Pemeriksaan Angka Lempeng Total digunakan untuk menentukan jumlah kuman yang terdapat di dalam sampel yang diperiksa. Hasil penelitian pada pendiaman 1 minggu jumlah Angka Lempeng Total 3,9 x 105 CFU/gr, 2 minggu jumlah Angka Lempeng Total 4,7 x 106 CFU/gr, dan 3 minggu jumlah Angka Lempeng Total 7,56 x 107 CFU/gr. Penelitian pada 1 minggu dibawah batas ambang SNI, dan penelitian 2 sampai 3 minggu diatas batas ambang ketentuan SNI yang tidak memenuhi syarat mutu pangan, sehingga tidak layak dikomsumsi.
Manfaat Prebiotik Tepung Pisang Kepok (Musa paradisiaca formatypica) terhadap Pertumbuhan Probiotik Lactobacillus casei secara In Vitro Hardisari, Ratih; Amaliawati, Nur
Jurnal Teknologi Laboratorium Vol 5 No 2 (2016): 2016 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

This background reviewed cases of diarrhea, one of health problems related to the digestive tract and caused by an imbalance in intestinal microflora. Probiotics are microorganisms that can be used to balance the intestinal microflora’s population. Prebiotics are nutrients required for the growth of good bacteria in human’s intestine. Kepok banana (Musa paradisiaca formatypica) that contains inulin and FOS is a natural source of prebiotics. The purpose of this study is to observe the effect of various concentrations of kepok banana flour (Musa paradisiaca formatypica) on the growth of probiotic bacterium Lactobacillus casei. In order to observe the prebiotic activity of Lactobacillus casei, post-test with control group design method is used toward the kepok banana flour. The amounts of kepok banana flour concentration used are varied, which are 2%, 4%, 6%, 8 %, and 10%. As much as 30 data was resulted from this research that was, later, descriptively analyzed with One-Way ANOVA test using SPSS 16.0 for Windows. The research’s finding shows the average numbers of bacterium Lactobacillus casei (the number of bacteria x 107CFU/ml) produced in the media with the addition of MPF’s concentrations of 2%, 4%, 6%, 8%, and 10% are, respectively, 29187,5 ; 42491,67 ; 52225 ; 89879,17 and 155108,33. This research also obtained a significance value of 0.000 of the One-way Anova test. The conclusion of this study is that there is an in vitro effect of adding various concentrations of kepok banana flour on the growth of probiotic bacterium Lactobacillus casei.
Co-Authors Dewi Apriani, Dewi Eni Kurniati, Eni Muhammad Rivai Ratih Hardisari, Ratih Saptono Putro