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Pemanfaatan Sari Ubi Jalar Ungu (Ipomoea batatas poiret) Sebagai Zat Pewarna Pada Pewarnaan Staphylococcus aureus yuniarty, Tuty; Misbach, Siti Rachmi
Jurnal Teknologi Laboratorium Vol 5 No 2 (2016): 2016 (2)

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The sweet potato purple (Ipomoea batatas poiret) is a type of tubers which have a component of starch, consisted of 30-40% amylase and 60-70% amylopectin. In addition, purple sweet potato also contains a lot of antioxidants derived from anthocyanins, which amounted to 110.51 mg/100 g, phenolic compounds contained in anthocyanin pigments which can stain the cell wall is peptidoglycan of gram-positive bacteria, Staphylococcus aureus. The purpose of this research is to determine whether the purple sweet potato essence can be used as an alternative to the staining of Staphylococcus aureus. This study is a Quasi-experimental design, results are compared directly between gram staining results of Staphylococcus aureus on gram staining using purple sweet potato essence as an experimental group and the gram staining using gentian violet as the control group. The results showed the bacteria with coccus form and reddish violet color presented on gram staining of the experimental group and the control group. The conclusion is the essence of purple sweet potato can be used as an alternative to the staining of Staphylococcus aureus.
Uji Daya Hambat Sari Daun Alpukat (Persea americana mill) terhadap Pertumubuhan Escherichia coli Yuniarty, Tuty; Hasjim, Lisfaresliana
Health Information : Jurnal Penelitian Vol 9 No 2 (2017): Digitalisasi Versi Cetak
Publisher : Poltekkes Kemenkes Kendari

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Avocado leaves (Percea americana mill) are a part of avocado plants that have benefits as traditional medicines. Avocado leaves are potentially used as anti-diarrhea based on the content of chemicals contained therein, namely saponins, alkaloids, tannins, flavanoids, polyphenols, quercetin which are used to kill pathogenic bacteria, one of which is Escherichia coli. Escherichia coli is an opportunist germ that is commonly found in the human intestine as a normal flora. The purpose of this study was to determine the inhibition of avocado leaf extract on the growth of Escherichia coli bacteria. This type of research is an Experimental laboratory. The research design used in this study was static group comparison because this study was conducted to see differences in concentrations of 10%, 15%, 25%, 50% and 75% of avocado leaf extract in inhibiting the growth of Escherichia coli bacteria by looking at the clear zone formed. The results showed that at a concentration of 25%, 50% and 75% a clear zone (inhibition zone) was formed, whereas at concentrations of 10% and 15% no clear zone (inhibition zone) was formed. From the results of this study it can be concluded that avocado leaf extract can inhibit the growth of Escherichia coli at concentrations of 25%, 50% and 75%.
Penataan Penjualan Ikan Asin Bebas Formalin sebagai Makanan Khas Masyarakat Kota Kendari Menuju Produk Unggulan Yang Sehat dan Higienis: (Studi Kasus: Pasar Tradisional dan Modern Kota Kendari) Darmayani, Satya; Hasnah, Nur; Yuniarty, Tuty; Yunus, Reni
Journal of Community Development Articles In Press
Publisher : Indonesian Journal Publisher

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Community services are focused on getting healthy and hygienic food sold in the city of Kendari. In the initial stage, the examination is carried out by taking samples of salted fish sold in traditional and modern markets and then tested whether they contain formaldehyde. If the findings of salted fish contain formaldehyde, education and guidance will be given about alternative formalin ingredients that are safe for health. A total of 60 samples of salted fish were taken from 7 traditional markets in Kendari city and 2 modern markets. The formalin content of fish was analyzed qualitatively. The results of this analysis showed 6 samples or about 10% contained formaldehyde. Based on the results of the analysis of 60 samples, it was found positive results in traditional markets and negative results for samples from modern markets. Thus, the Kendari City Traditional Market has stated that salted fish contains formaldehyde and is very dangerous if consumed continuously.
Gambaran Angka Kematian Larva Nyamuk Aedes aegypti dengan Pemberian Kulit Jeruk Purut (Citrus hystrix) Sebagai Larvasida Alami Yuniarty, Tuty; Yunus, Reni
Jurnal Teknologi Kesehatan (Journal of Health Technology) Vol. 12 No. 2 (2016): September

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Dengue Fever (DF) and Dengue Haemorrhagic Fever (DHF) are infectious diseases caused by Dengue Virus and transmitted by the bite of Aedes Aegypti. One way to prevent the spreading of the DHF is by controlling its vectors. The best and most effective way to control the vector is with the eradication of Aedes aegypti larvae, which is called larviciding using temephos (abate powder). However, the application of temephos continually will pollute the environment and increase the resistance of larvae to pesticide so that natural pesticides are needed. One of them is by using Kaffir lime peel (Cytrushystric) which contains limonoids. Limonoid is known as toxic to the larvae of Aedes aegypti. The objective of this research is to find out the mortality rate of larvae with the concentrations of Kaffir lime peel as a natural pesticide. The method of this research is descriptive research, by using 200 Aedes aegypti larvae. Those larvae were divided into 3 concentrations of 3%, 5% and 7% of Kaffir lime peel with twice repetition and 1 % of temephos as a control. Each concentration contained 25 larvae in 100 ml of water. The observations were conducted by counting the dead larvae. The results showed that the average of the mortality rate of larvae at a concentration of 3% was 9 larvae (36%), a concentration of 5% were 21 of larvae (84%) and 7% were 24 of larvae (96%). It can be concluded that the mortality rate of Aedes Aegypti larvae by using Kaffir lime as natural larvacide at the concentration of 7% was the highest out of 3 concentrations with mortality of larvaewere 24 of 25 larvae (96%).