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Isolasi dan Identifikasi Bakteri Termofilik Penghasil Enzim Selulase dari Sumber Air Panas Sila Laury M. Ch. Huwae; Pingkan Aditiawati
ACROPORA: Jurnal Ilmu Kelautan dan Perikanan Papua Vol 3, No 1 (2020)
Publisher : ACROPORA: Jurnal Ilmu Kelautan dan Perikanan Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (675.058 KB) | DOI: 10.31957/acr.v3i1.1214

Abstract

Cellulose is included in the main polysaccharide group that is widely available in nature. Cellulose is a straight-chain glucose polymer that is bound by β-1,4 glucoside bonds. Cellulase is an enzyme complex consisting of several enzymes that work in stages or together in breaking down cellulose into glucose, by hydrolyzing the β-1,4 glucoside bond in cellulose. Cellulase enzymes have been used in various industries. Industrial applications require enzymes that are stable at high temperatures. Various types of thermostable enzymes can be isolated from thermophilic bacteria, cellulase enzymes are no exception. This study aims to isolate and identify thermophilic bacteria that produce cellulase enzymes from Sila hot springs. The results of isolation and screening of cellulase-producing bacteria from hot water samples obtained by 7 isolates that have the ability to degrade cellulose can produce clear zones on LB-CMC agar selective medium, but CR2 isolates showed the largest zone of 1.5 cm. CR2 isolates were Gram-positive bacteria and were identified as having close relations with Bacillus aryabhattai strain B8W22 using the 16S rRNA method.Keywords: Cellulose; Cellulose enzyme; Thermostable enzyme; Thermophilic bacteria
Struktur Komunitas Bulu Babi Di Perairan Pantai Kampung Baru Pulau Banda Kabupaten Maluku Tengah Deli Wakano; Laury Marcia Chara Huwae
BIOSEL (Biology Science and Education): Jurnal Penelitian Science dan Pendidikan Vol 9, No 2 (2020): BIOSEL (Biology Science and Education): Jurnal Penelitian Science dan Pendidikan
Publisher : INSTITUT AGAMA ISLAM NEGERI AMBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (860.988 KB) | DOI: 10.33477/bs.v9i2.1628

Abstract

The waters of Kampung Baru Village are one of the waters in Banda Island, Central Maluku Regency, which has an abundance of marine life. The substrate conditions in these waters are rocky, rocky and sandy, surrounded by seagrass. On the coast of Kampung Baru Village, there are many marine biota, including crustaceans, gastropods, bivalves, echinoderms, sea cucumbers, starfish and others. Sea urchins are one of the most important biota because people in Kampung Baru Village use their gonads for daily consumption. The existence of sea urchins in coral reef ecosystems has a significant effect on the ecological balance. The purpose of this study was to determine the community structure of sea urchins in the coastal areas of Kampung Baru, Central Maluku Regency. It is necessary to do so so that the presence of sea urchins can be known so that the balance is well monitored. The method used is a quadratic linear transect. The results showed that the diversity of sea urchin species on the coast of the village of Kampung Baru, Banda Island, Central Maluku was 0.815, including the low category. The value of dominance is 0.512, which means there is a tendency for species to dominate. The density value is 0.065 and the distribution value is 0.277 which is included in the uniform category. Keywords: Community Structure, Sea Urchins, Kampung Baru, Banda Island
PENDIDIKAN MITIGASI BENCANA GEMPA BUMI KEPADA SISWA KATEKISASI DI SEKTOR CALVARY JEMAAT GPM REHOBOTH Laury Marcia Chara Huwae; Ronald Darlly Hukubun; Wiwien Gaby Hukubun
PAKEM : Jurnal Pengabdian Kepada Masyarakat Vol 2 No 1 (2022): Pakem : Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (685.25 KB) | DOI: 10.30598/pakem.2.1.18-23

Abstract

Disaster mitigation is an effort that can be done to prevent casualties and minimize losses, this is in line with the condition of Maluku which is prone to earthquakes. This PKM aims to provide knowledge and understanding of earthquake disaster mitigation. The service method is presented in the form of providing material, discussion and evaluation. It is hoped that the targeted catechism students (partners) can become “agents of change” in sharing information and knowledge with the general public about the importance of disaster emergency preparedness
Inventarisasi Jenis Burung Di Habitat Hutan Desa Waai Kabupaten Maluku Tengah Veince Benjamin Silahooy; Laury Marcia Chara Huwae; Kresyan Pentury
Biosfer : Jurnal Biologi dan Pendidikan Biologi Vol 5 No 1 (2020): BIOSFER: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP Unpas,

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (248.745 KB) | DOI: 10.23969/biosfer.v5i1.2566

Abstract

Birds are an important component in a forest ecosystem. Birds play a role in pollination, seed dispersers, pest control, and even their droppings provide nutrients for the soil. After the earthquake in Ambon and surrounding areas in September 2019, many people fled to the forest. This research was conducted in Waai village to inventory the species of birds in the forest (HA) and forests that were converted into post-earthquake refuge (HP) using the list of bird species and natural sciences. The results show that 8 species of birds were found at the HA location while 3 species of birds were found at the HP location. The highest species abundance was Passer montanus at both locations. The lowest abundance value on HA is Todirhamphus chloris and Ducula bicolor, while on HP is Leptocoma aspasia. Diversity index analysis shows 1.67 index on HA and 0.86 on HP.
IDENTIFIKASI KARAKTER MORFOLOGI TURPEPEL (Cuora amboinensis) DI SUNGAI WAIMAMOKANG, DESA HALONG PULAU AMBON Veince Benjamin Silahooy; Laury Marcia Chara Huwae
Biofaal Journal Vol 1 No 2 (2020): Biofaal Journal
Publisher : Jurusan Biologi FMIPA Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (333.745 KB) | DOI: 10.30598/biofaal.v1i2pp107-111

Abstract

C. amboinensis dalam Bahasa lokal Maluku disebut Turpepel, sering dilaporkan kemunculannya di hutan sepanjang DAS waimamokang Desa Halong. Penurunan populasi hingga 50-80% dalam 20 tahun terakhir mengakibatkan perlu evualuasi kembali dan hasilnya menempatkan spesies ini masuk dalam kategori konservasi lebih tinggi yaitu Endangered (EN). Analisa data dilakukan secara deskripif dengan melakukan pengukuran langsung pada karapas, plastron, kepala, kaki dan massa dari setiap individu. Deskriptif karakter morfologi dijelaskan secara akurat sesuai dengan fakta yang ditemukan di lapangan. Hasil penelitian ini ditemukan 7 individu Turpepel (C. amboinensis) dimana 6 diantaranya berkelamin jantan dan 1 berkelamin betina. Karapas tersusun atas lempengan yang keras berbentuk segi enam yang simetris vertikal. Plastron dapat digerakan terutama pada bagian yang dekat dengan kepala dan ekor. Seluruh bagian kepala dapat disembunyikan, dan tidak ada perbedaan yang terlalu signifikan antara panjang dan lebar pada kaki depan dan kaki belakang
PENINGKATAN KEBERHASILAN IMPLANTASI TIKUS Rattus norvegicus TERPAPAR ASAP ROKOK PASCA DITERAPI EKSTRAK ETANOL RUMPUT KEBAR (Byophytum petersianum Klotzsch) Jeanny Stevani Rahandity; Adrien Jems Akiles Unitly; La Eddy; Laury Marcia Chara Huwae
Biofaal Journal Vol 2 No 1 (2021): Biofaal Journal
Publisher : Jurusan Biologi FMIPA Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (264.913 KB) | DOI: 10.30598/biofaal.v2i1pp19-27

Abstract

Penelitian ini bertujuan untuk mengetahui peningkatan keberhasilan implantasi tikus Rattus norvegicus terpapar asap rokok pasca diterapi ektrak etanol rumput kebar (Biophytum petersianum Klotzsch). Penelitian mengggunakan metode eksperimental laboratorik dengan rancangan acak lengkap (RAL) yang terdiri dari empat perlakuan yang masing-masing diulang tiga kali, yaitu (-): Kelompok kontrol negatif yaitu tikus yang tidak diberi perlakuan, (+): Kelompok kontrol positif yaitu tikus dipaparkan asap rokok selama 28 hari, (0.067): Kelompok tikus yang dipapar asap rokok selama 28 hari kemudian diberi ekstrak etanol rumput kebar dengan dosis 0.067mg/ekor/hari selama 28 hari, dan (0.135): Kelompok tikus yang dipapar asap rokok selama 28 hari kemudian diberi ekstrak etanol rumput kebar dengan dosis 0.135mg/ekor/hari selama 28 hari. Setelah itu, semua tikus dikawinkan. Pengamatan jumah korpus luteum pada organ ovarium dan titik implantasi pada organ uterus tikus dilakukan pada usia kebuntingan 12 hari. Data yang dianalisis dengan analysis of variance (ANOVA)-SPSS versi 16.0 dilanjutkan dengan uji Duncan dengan selang kepercayaan 95% (α = 0.05) dan metode nonparametric. Hasil penelitian menunjukkan bahwa pemberian ekstrak etanol rumput kebar (Byophytum petersianum Klotzsch) dengan dosis 0.135 mg/ekor/hari mampu meningkatkan keberhasilan implantasi tikus Rattus norvegicus.
Utilization Of Activated Charcoal From Nutmeg Shell Waste As An Adsorbent To Improve The Quality Of Virgin Coconut Oil (VCO) Fermentation Method Hendro Hitijahubessy; Laury Marcia Ch Huwae
Biofaal Journal Vol 2 No 2 (2021): Biofaal Journal
Publisher : Jurusan Biologi FMIPA Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (452.599 KB) | DOI: 10.30598/biofaal.v2i2pp81-86

Abstract

Virgin coconut oil (VCO) is a form of processed coconut that is widely produced by people in Indonesia. VCO is often called pure coconut oil. VCO with good quality is by fermentation method using yeast. The quality of VCO can be measured by 3 types of analysis: water content, free fatty acids and peroxide number. The results of this analysis will be compared with the standards of the Asian and Pacific Coconut Community (APCC). VCO from fermentation method will be combined with the absorption method using nutmeg shell activated charcoal to see if there is a much better quality improvement. The results showed that there was a significant increase in quality with a decrease in the average water content from 0,1665% to 0,1005%. In addition, there was a significant decrease in the average free fatty acid content from 0,4681% to 0,3790% and significant decrease in average peroxide number from 0,1991 meq/kg to 0,0997 meq/kg. The results of the research on three VCO quality standards showed a value that was in accordance with the APCC standard.
SELEKTIFITAS PERTUKARAN ION Mg-K AKIBAT PEMBERIAN LARUTAN NH3 PADA TANAH RENZINA DENGAN BEBERAPA WAKTU INKUBASI Charles Silahooy; Veince Benjamin Silahooy; Laury Marcia Ch Huwae
Biofaal Journal Vol 3 No 1 (2022): Biofaal Journal
Publisher : Jurusan Biologi FMIPA Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v3i1pp12-18

Abstract

The cation exchange on the adsorbtion complex constitutes an important chemical character of soil in determining soil fertility level. To see which ion that more selective to be separated by NH4+ ion complex absorbtion between Mg and K. The expereiment was done at soil laboratory, faculty of agriculture, pattimura university ambon. The expereiment applied completely randomized desighn. With factorial pattern. NH3 solution treatment consist of 3 hour, 7 and 14 days. the result of the expereiment showed that there was an interaction between NH3 solution treatment and incubation time on the selectivity interchange of Mg-K ion, the availability rate of K and Mg, the change of pH and the conductivity of electricity . the addition of NH3 solution increased soil ph, DHL, Exchangable Mg, exchangeable k, Mg2+ and k+ ions in the soil solution and effected selectivity of Mg-k ion interchange.
UJI AKTIVITAS ANTIBAKTERI Vibrio sp. DARI DAUN SERNAI (Wedelia biflora) Hendro Hitijahubessy; Aprianti Samid; Welda Kristiyanti Jalmaf; Nurani Hasanela; Laury Marcia Ch Huwae
Biofaal Journal Vol 3 No 1 (2022): Biofaal Journal
Publisher : Jurusan Biologi FMIPA Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v3i1pp43-50

Abstract

Penyakit Vibriosis pada ikan kakap, udang vaname dan rumput laut sering disebabkan disebabkan oleh bakteri Vibrio sp. Penyakit tersebut dapat dideteksi dengan mengisolasi bakteri dan menanamnya pada media agar selektif Vibrio sp. yaitu Thiosulfate Citrate Bile Sucrose (TCBS) agar. Daun sernai (Wedelia biflora) adalah tumbuhan yang banyak diteliti dan berguna sebagi antimikroba atau antibakteri. Berdasarkan kegunaan sebagai anti bakteri diharapkan dalam penelitian ini bahwa daun sernai dapat menjadi antibakteri Vibrio sp. Efektifitas dan efisiensi dari daun sernai sebagai antibakteri Vibrio sp. dalam penelitian ini dapat diperoleh dengan menentukan rendemen ekstrak, analisis fitokimia dan uji aktifitas ekstrak sebagai antibakteri Vibrio sp dengan metode disc diffusion Kirby-Bauer. Rendemen ekstrak daun sernai yang dihasilkan sebesar 11,7 %, dengan kandungan metabolit sekunder hasil uji fitokimia serbuk daun sernai menunjukan positif adanya senyawa alkaloid, terpenoid, flavonoid, dan saponin. Ekstrak daun sernai dengan konsentrasi 100 % mampu menghambat bakteri dengan respon hambat pertumbuhan bakteri Vibrio sp. yang kuat atau sensitif dan mempunyai respon hambat yang sama dengan tetrasiklin 30 mg.
PENGENALAN LINGKUNGAN PESISIR DAN LAUT BAGI SISWA SMP NEGERI 1 ATAP SATHER, KABUPATEN MALUKU TENGGARA Wiwien Gaby Hukubun; Laury Marcia Chara Huwae
PAKEM : Jurnal Pengabdian Kepada Masyarakat Vol 2 No 2 (2022): Pakem : Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/pakem.2.2.90-95

Abstract

Coastal villages have direct access to coastal ecosystems, mangroves, seagrass beds, and coral reef ecosystems. Socio-ecologically, coastal villages have a linkage and dependence between coastal community activities and the existence of ecosystems/ecology. Knowledge of coastal areas is important for the people living around the area. Community Service is focused on teenagers/youth as part of the community with an educative counseling approach to the students of SMP Negeri Satu Atap Sather which aims to foster a sense of love and care for the coastal area and the ecosystem in it. Service activities went well. More intense and periodic education is needed to instill a caring attitude and a sense of love for the coastal and marine environment, so that coastal and marine resources can be maintained and sustainable